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The map label for this gene is dnaE [H]

Identifier: 158333930

GI number: 158333930

Start: 722491

End: 723831

Strand: Direct

Name: dnaE [H]

Synonym: AM1_0744

Alternate gene names: 158333930

Gene position: 722491-723831 (Clockwise)

Preceding gene: 158333929

Following gene: 158333931

Centisome position: 11.11

GC content: 49.96

Gene sequence:

>1341_bases
GTGGTGAAGATAATTCAACGTCGCTCCCTTGGCCTCCAGTCCGTTTATGACATCGGTCTCGCCCAAGATCATAATTTTGT
GATGGCCAATGGTTGGGTTGCGGCCAACTGCTTTAATAAATCTCACTCCACCGCCTATGGATATGTCACCTATCAAACCG
CCTATTTAAAAGCCAACTACCCCGTTGAGTATATGGCGGCCCTACTCACCGCCAATAGTGGTGATCAGGATAAAGTACAG
AAGTACATTGCCACGGCCCTCAGCATGGGGATTGAAGTGGAACCGCCTGATATCAACCGTTCCGGCGTGGATTTCACCCC
CTTAGACAAAAATATTTTGTTTGGACTGTCTGCGGTTCGTAATTTGGGGCAAGGAGCCATTGAAGCCATTTTGGCAGCTC
GGGATGAGGATGGGGGGTTTGAGTCTTTGGCAGAGCTTTGCGATCGGGTTGATCCACGAGTGGTTAATCGGCGGGCATTA
GAAGCCCTAATCTATAGCGGCGCTCTGGATGCCTTTGACACCAATCGCAATCAGTTAGTCCATGATTTAGAACTCATTCT
GGAATGGGCCCAGTCCCGGGCGAAGGATCGAGCGATAGGTCAGGGCAATCTCTTTGACATCATGGGCGGCGAAGGCGATG
CACCTACCTCTTTAGAAACAGCCCCTAAAGCCGAACCCGTTGAAGATTTTCAAGAATCCGAAAAGCTGAAGCTGGAAAAA
GAAATCCTTGGCTTCTATATTTCCAACCATCCCTTGCGAGAAATTGAGCGGCCCGCTCGGCTGATGGCTCCCATCAGTTT
GGCGAATTTAGAAGAACAGCCCGAAAAAACCACCGTCAGTGCCATTGTGTTACTCACGGAAGTCAAGCCGATTATTACCA
AGAAAGGCGATCGGATGGCGATCGTGCAGATGGAAGACTTGTCGGGGAAGGCAGAAGGCGTCGTCTTTCCTCGAAGCTTT
GAGCGGATTGGTGAGTACATTGAGACGGACCTCCGCCTAATGATTTGGGGGAAAGTAGATCGGCGAGACGATCGCATTCA
GATCATTGTTGAAGATGCCGAGCCGATCGAAGAGGTGAAGCTTGTTATGGTGGAAATTCCGGCTCAAGATGCCGGTAATA
TCCAGCAACAGCAACGGCTCAAGGAAATTTTGCGACAGCAAGCTGGAGAGGAAAACGCCGCCAAAGTATCTGTCATTGCC
AAAATTAAGGGAGATGAAAAAAGTCAGTTGGTTCGTCTGGGGTCCCAGTTCCGAGTCCAAAATGAGCACATGGCGGTTCA
AGCTCTTGCCAGTGCTGGCTTTCGCGCATCCTCTTCGGTGTTGGTTGATCTAAAAACCTGA

Upstream 100 bases:

>100_bases
CCGATCATCCCAACGAACGAGGCGAAATTTTAGGATTAAGATGGTGAGAACCTGACCTGCCCGCATCCTTCCTATGGCGT
CAATCTAGGATCAAAATACT

Downstream 100 bases:

>100_bases
AAAAAATATTTGCGGATAATTTTAATTTCGCAAAAATATGCATAATATAGCTGGTGGACATGAATATTTCACCGCTAGAC
CTAGGCATATGCAAATTTCT

Product: DNA polymerase III subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MVKIIQRRSLGLQSVYDIGLAQDHNFVMANGWVAANCFNKSHSTAYGYVTYQTAYLKANYPVEYMAALLTANSGDQDKVQ
KYIATALSMGIEVEPPDINRSGVDFTPLDKNILFGLSAVRNLGQGAIEAILAARDEDGGFESLAELCDRVDPRVVNRRAL
EALIYSGALDAFDTNRNQLVHDLELILEWAQSRAKDRAIGQGNLFDIMGGEGDAPTSLETAPKAEPVEDFQESEKLKLEK
EILGFYISNHPLREIERPARLMAPISLANLEEQPEKTTVSAIVLLTEVKPIITKKGDRMAIVQMEDLSGKAEGVVFPRSF
ERIGEYIETDLRLMIWGKVDRRDDRIQIIVEDAEPIEEVKLVMVEIPAQDAGNIQQQQRLKEILRQQAGEENAAKVSVIA
KIKGDEKSQLVRLGSQFRVQNEHMAVQALASAGFRASSSVLVDLKT

Sequences:

>Translated_446_residues
MVKIIQRRSLGLQSVYDIGLAQDHNFVMANGWVAANCFNKSHSTAYGYVTYQTAYLKANYPVEYMAALLTANSGDQDKVQ
KYIATALSMGIEVEPPDINRSGVDFTPLDKNILFGLSAVRNLGQGAIEAILAARDEDGGFESLAELCDRVDPRVVNRRAL
EALIYSGALDAFDTNRNQLVHDLELILEWAQSRAKDRAIGQGNLFDIMGGEGDAPTSLETAPKAEPVEDFQESEKLKLEK
EILGFYISNHPLREIERPARLMAPISLANLEEQPEKTTVSAIVLLTEVKPIITKKGDRMAIVQMEDLSGKAEGVVFPRSF
ERIGEYIETDLRLMIWGKVDRRDDRIQIIVEDAEPIEEVKLVMVEIPAQDAGNIQQQQRLKEILRQQAGEENAAKVSVIA
KIKGDEKSQLVRLGSQFRVQNEHMAVQALASAGFRASSSVLVDLKT
>Mature_446_residues
MVKIIQRRSLGLQSVYDIGLAQDHNFVMANGWVAANCFNKSHSTAYGYVTYQTAYLKANYPVEYMAALLTANSGDQDKVQ
KYIATALSMGIEVEPPDINRSGVDFTPLDKNILFGLSAVRNLGQGAIEAILAARDEDGGFESLAELCDRVDPRVVNRRAL
EALIYSGALDAFDTNRNQLVHDLELILEWAQSRAKDRAIGQGNLFDIMGGEGDAPTSLETAPKAEPVEDFQESEKLKLEK
EILGFYISNHPLREIERPARLMAPISLANLEEQPEKTTVSAIVLLTEVKPIITKKGDRMAIVQMEDLSGKAEGVVFPRSF
ERIGEYIETDLRLMIWGKVDRRDDRIQIIVEDAEPIEEVKLVMVEIPAQDAGNIQQQQRLKEILRQQAGEENAAKVSVIA
KIKGDEKSQLVRLGSQFRVQNEHMAVQALASAGFRASSSVLVDLKT

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase [H]

COG id: COG0587

COG function: function code L; DNA polymerase III, alpha subunit

Gene ontology:
GO:0003677: DNA polymerase III alpha subunit
GO:0003824: DNA polymerase III alpha subunit
GO:0003887: DNA polymerase III alpha subunit
GO:0005737: DNA polymerase III alpha subunit
GO:0006260: DNA polymerase III alpha subunit
GO:0008408: DNA polymerase III alpha subunit
GO:0016539: DNA polymerase III alpha subunit
GO:0016740: DNA polymerase III alpha subunit
GO:0016779: DNA polymerase III alpha subunit
GO:0003676: DNA polymerase III alpha subunit, putative

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-C family. DnaE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786381, Length=394, Percent_Identity=32.2335025380711, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR003587
- InterPro:   IPR006141
- InterPro:   IPR004365
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR016195
- InterPro:   IPR004805 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF02811 PHP; PF01336 tRNA_anti [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 49472; Mature: 49472

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50818 INTEIN_C_TER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIIQRRSLGLQSVYDIGLAQDHNFVMANGWVAANCFNKSHSTAYGYVTYQTAYLKANY
CCEEECHHCCCHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCCEEEEEEEEEEEEEECC
PVEYMAALLTANSGDQDKVQKYIATALSMGIEVEPPDINRSGVDFTPLDKNILFGLSAVR
CHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHH
NLGQGAIEAILAARDEDGGFESLAELCDRVDPRVVNRRALEALIYSGALDAFDTNRNQLV
HCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
HDLELILEWAQSRAKDRAIGQGNLFDIMGGEGDAPTSLETAPKAEPVEDFQESEKLKLEK
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EILGFYISNHPLREIERPARLMAPISLANLEEQPEKTTVSAIVLLTEVKPIITKKGDRMA
HHHHHHHCCCCHHHHHCHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHCCHHHHCCCCEEE
IVQMEDLSGKAEGVVFPRSFERIGEYIETDLRLMIWGKVDRRDDRIQIIVEDAEPIEEVK
EEEEECCCCCCCCEECCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCCHHHEE
LVMVEIPAQDAGNIQQQQRLKEILRQQAGEENAAKVSVIAKIKGDEKSQLVRLGSQFRVQ
EEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHCHHHEEC
NEHMAVQALASAGFRASSSVLVDLKT
CHHHHHHHHHHCCCCCCCCEEEEECC
>Mature Secondary Structure
MVKIIQRRSLGLQSVYDIGLAQDHNFVMANGWVAANCFNKSHSTAYGYVTYQTAYLKANY
CCEEECHHCCCHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCCEEEEEEEEEEEEEECC
PVEYMAALLTANSGDQDKVQKYIATALSMGIEVEPPDINRSGVDFTPLDKNILFGLSAVR
CHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHH
NLGQGAIEAILAARDEDGGFESLAELCDRVDPRVVNRRALEALIYSGALDAFDTNRNQLV
HCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
HDLELILEWAQSRAKDRAIGQGNLFDIMGGEGDAPTSLETAPKAEPVEDFQESEKLKLEK
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EILGFYISNHPLREIERPARLMAPISLANLEEQPEKTTVSAIVLLTEVKPIITKKGDRMA
HHHHHHHCCCCHHHHHCHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHCCHHHHCCCCEEE
IVQMEDLSGKAEGVVFPRSFERIGEYIETDLRLMIWGKVDRRDDRIQIIVEDAEPIEEVK
EEEEECCCCCCCCEECCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCCHHHEE
LVMVEIPAQDAGNIQQQQRLKEILRQQAGEENAAKVSVIAKIKGDEKSQLVRLGSQFRVQ
EEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHCHHHEEC
NEHMAVQALASAGFRASSSVLVDLKT
CHHHHHHHHHHCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231; 9689062; 10734038; 11170467 [H]