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The map label for this gene is insB1 [H]

Identifier: 158333928

GI number: 158333928

Start: 720392

End: 721081

Strand: Direct

Name: insB1 [H]

Synonym: AM1_0742

Alternate gene names: 158333928

Gene position: 720392-721081 (Clockwise)

Preceding gene: 158333925

Following gene: 158333929

Centisome position: 11.08

GC content: 45.94

Gene sequence:

>690_bases
ATGAAGTGTCCTGAGTGTGGTGGTCAACATATCCATAAGAATGGGCATCGTCGAGGTAAACAAAATCATATCTGTGTGAC
CTGTGGACGACAGTTTCTTTCAACTTACTCAAAGCGTGGCTACAGTGATTGGACGAAACGACTCTGCCTGAGAATGTATG
TCAATGGAATGGGGTTGAAGGGTATCGAACGAGTCATAGGCGTTGCTCATACCACTGTGATTCATTGGGTTCAACAAGTT
GGAGTTCTATTACCCGATGCTTATGACCCCGACGATATCCCACAGGTCGGAGAATTAGATGAACTCGAAACCTTTGTGGG
CAAAAAACGCAATAAGGTCTGGATTTGGACTGTGGTGGATCATTTTCACCCCGGCATTCTAGGCTGGGTTGTGGGTAACC
ATAGTGCTGAGACCTTTCGACCGTTATGGCAAGCAATCGTCTGTTGGCAATGTTTTTTCTGGGTCAGTGATGGCAATCCC
GTCTATCCAGGCTTTATCCCAGAGGGTGACCAGATTGTTAGCAAGACCTACATGACACGAGTAGAGGGAGAAAATACTCG
TTTACGACATTACTTGGCACGGCTCCATCGCAAGACTTTATGTTATTCAAAGTCGGTGGACATGCTCAAGCATTCAATTC
GACTATTGATCCACTATCTCAAGTTTGGGGAAGTACCTATTCCTGCGTGA

Upstream 100 bases:

>100_bases
TGGTGTCCTGCTGCTATTGGCGTTGCTCAATGACGGGATGAATTAATTGGATTGCGTCAGGATAACCTCATGACTATTTC
AAACATCATGAGGAATCTGT

Downstream 100 bases:

>100_bases
GTCATCCCGTCATTGAGCAACGCCGCTGCTATTTGCTGAGTTAATTTCCCCTCCATCAATCGCGTGGAGGTTCAACCTGA
TTGATGTTCACTTTGGGAAC

Product: IS1 transposase

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKCPECGGQHIHKNGHRRGKQNHICVTCGRQFLSTYSKRGYSDWTKRLCLRMYVNGMGLKGIERVIGVAHTTVIHWVQQV
GVLLPDAYDPDDIPQVGELDELETFVGKKRNKVWIWTVVDHFHPGILGWVVGNHSAETFRPLWQAIVCWQCFFWVSDGNP
VYPGFIPEGDQIVSKTYMTRVEGENTRLRHYLARLHRKTLCYSKSVDMLKHSIRLLIHYLKFGEVPIPA

Sequences:

>Translated_229_residues
MKCPECGGQHIHKNGHRRGKQNHICVTCGRQFLSTYSKRGYSDWTKRLCLRMYVNGMGLKGIERVIGVAHTTVIHWVQQV
GVLLPDAYDPDDIPQVGELDELETFVGKKRNKVWIWTVVDHFHPGILGWVVGNHSAETFRPLWQAIVCWQCFFWVSDGNP
VYPGFIPEGDQIVSKTYMTRVEGENTRLRHYLARLHRKTLCYSKSVDMLKHSIRLLIHYLKFGEVPIPA
>Mature_229_residues
MKCPECGGQHIHKNGHRRGKQNHICVTCGRQFLSTYSKRGYSDWTKRLCLRMYVNGMGLKGIERVIGVAHTTVIHWVQQV
GVLLPDAYDPDDIPQVGELDELETFVGKKRNKVWIWTVVDHFHPGILGWVVGNHSAETFRPLWQAIVCWQCFFWVSDGNP
VYPGFIPEGDQIVSKTYMTRVEGENTRLRHYLARLHRKTLCYSKSVDMLKHSIRLLIHYLKFGEVPIPA

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG3677

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=118, Percent_Identity=31.3559322033898, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788203, Length=118, Percent_Identity=31.3559322033898, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1786203, Length=118, Percent_Identity=31.3559322033898, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1787223, Length=118, Percent_Identity=31.3559322033898, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1786470, Length=130, Percent_Identity=31.5384615384615, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786459, Length=130, Percent_Identity=31.5384615384615, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 26441; Mature: 26441

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCPECGGQHIHKNGHRRGKQNHICVTCGRQFLSTYSKRGYSDWTKRLCLRMYVNGMGLK
CCCCCCCCHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHH
GIERVIGVAHTTVIHWVQQVGVLLPDAYDPDDIPQVGELDELETFVGKKRNKVWIWTVVD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEHH
HFHPGILGWVVGNHSAETFRPLWQAIVCWQCFFWVSDGNPVYPGFIPEGDQIVSKTYMTR
HHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCEECCCCCCCCHHHHHHHHHHH
VEGENTRLRHYLARLHRKTLCYSKSVDMLKHSIRLLIHYLKFGEVPIPA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKCPECGGQHIHKNGHRRGKQNHICVTCGRQFLSTYSKRGYSDWTKRLCLRMYVNGMGLK
CCCCCCCCHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHH
GIERVIGVAHTTVIHWVQQVGVLLPDAYDPDDIPQVGELDELETFVGKKRNKVWIWTVVD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEHH
HFHPGILGWVVGNHSAETFRPLWQAIVCWQCFFWVSDGNPVYPGFIPEGDQIVSKTYMTR
HHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCEECCCCCCCCHHHHHHHHHHH
VEGENTRLRHYLARLHRKTLCYSKSVDMLKHSIRLLIHYLKFGEVPIPA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]