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The map label for this gene is 158333851

Identifier: 158333851

GI number: 158333851

Start: 645741

End: 647207

Strand: Reverse

Name: 158333851

Synonym: AM1_0661

Alternate gene names: NA

Gene position: 647207-645741 (Counterclockwise)

Preceding gene: 158333853

Following gene: 158333850

Centisome position: 9.95

GC content: 44.44

Gene sequence:

>1467_bases
ATGACAGCTATTGCATCAGAGAAATTAAGCAAAAACGCTTCTCTAGTCAAAGACTGGGCCAAAGGTTATGAATCACAGCT
GAATGAATATAGTTACTGGATTGATGAAGTAGAAGGCACCATTCCCCCAGAACTGCAAGGGACGTTGTTCCGGAATGGAG
CTGGCAGCTTAGAAGTCAACGGCCAAAAAATCGGCCATCCCTTTGATGGAGACGGCATGATCTGTGCCATCACCTTTGAG
CAAGGTCGTGCCCACTTTCAAAATCGCTACGTTCGCACAGAAGGCTATCTCAAAGAACAAGCCGCTGGGAAAATCCTATA
CCGAGGGTTTGGCACCCAAAAAGCGGGAGGATGGCTAGCCAATATTTTTAATACCAATTTCAAAAATGCGGCAAACACCA
GCGTGATTTACTGGGGAGATAAATTATGGGCGATGTGGGAAGGCGGCCATCCCCATCAGCTCACTCCTGAGACCCTAGAA
ACCATTGGTCCAGACACTCTAGATGGACTACTCACAGCCGATCAGCCTTTTTCGGCTCACCCCAGAATCATTGATGGCCG
TTTTATTAATTTTGGCGTTAAAGGGATTGCGTCCCAAAGCTTAACCATCTTTGAACTCGATGAGCAAGGCAACCCATTAA
AACAGTCCTCTCATCCTTTATCAGGGTTTGCTTTCTTGCACGACATGTTAGTAACAGAAAATTACTGCATCTTTGTGCAG
CATCCCTTTCAGGTAAAAGGATTGCCTTTTCTGCTGGGTTTCAAAACTATTGAACAATGTTTTGACTTCAATCCAGAGCA
ACCCACTAAAATCATTCTGATTTCTCGCCATGGCAATCATGACCTAGAGATATTAGAAACCGATTCATTCTTTGGATTTC
ACCATGGCAATGCCTGGGAGAAAGAAGGAAAACTCTACTTTGAATCCGTTTGTAGTGATTTCTTCCCCCAGAAACAGCAA
GACGAGTTAGATTTTGAGCAAATTGACTTTGAGTCTTTCCCCACAGGCGAATTATGGGAATTTGAGGTCAATTTATCATC
TAAAAAGGTAGTCCACCGAAAAGTAGTTGAAAGAGGCTGTGAATTTCCCTCCGTGCATCCCCAATGGGTAGGAAAAGAGC
ATCGTTATGTATATATGAGTGTCTGTGACAGTTCAGATAAAAATGGTCCCCTGCAAGCCATTCTTAAGCTCGATAAAGAA
TCCGGTGAACAGCAAATCTGGAGTGTCGCTCCCAGAGCATTTCCGGGTGAACCGATTTTTGTCCCTCGCCCCGATAGCAC
TCAAGAAGATGATGGGTGGTTACTCTCTCTCGTATTTGATGCCGCTACCTATCGTTCTTATTTGGCCATTTTAGATGCCC
AAGATCTGAACACCGTCATTGCAAAATTACACCTTCAGCATCATATTCCTCATGGATTCCACGGTAGCTGGACGCCTAAT
GTCTTCCGTGACTCTTCCAAGAATTAA

Upstream 100 bases:

>100_bases
TGTCATTTAATTCTGGCATGTACAACGCTACTTCAACATCGCTGCGATTTTGGCATAGTTTTTTCACGCTAAGATTATTA
CAAATCTTCGTAAAAATGGT

Downstream 100 bases:

>100_bases
CAACTGTACAGAAAAAAATAAGGATTGAATCGAAGAATTACCAGAAGCAGACTACATTAGAACTAGTCCCCAGCCCACCC
TGATTGCCCAAGGCGAAGCA

Product: lignostilbene-alpha,beta-dioxygenase

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTAIASEKLSKNASLVKDWAKGYESQLNEYSYWIDEVEGTIPPELQGTLFRNGAGSLEVNGQKIGHPFDGDGMICAITFE
QGRAHFQNRYVRTEGYLKEQAAGKILYRGFGTQKAGGWLANIFNTNFKNAANTSVIYWGDKLWAMWEGGHPHQLTPETLE
TIGPDTLDGLLTADQPFSAHPRIIDGRFINFGVKGIASQSLTIFELDEQGNPLKQSSHPLSGFAFLHDMLVTENYCIFVQ
HPFQVKGLPFLLGFKTIEQCFDFNPEQPTKIILISRHGNHDLEILETDSFFGFHHGNAWEKEGKLYFESVCSDFFPQKQQ
DELDFEQIDFESFPTGELWEFEVNLSSKKVVHRKVVERGCEFPSVHPQWVGKEHRYVYMSVCDSSDKNGPLQAILKLDKE
SGEQQIWSVAPRAFPGEPIFVPRPDSTQEDDGWLLSLVFDAATYRSYLAILDAQDLNTVIAKLHLQHHIPHGFHGSWTPN
VFRDSSKN

Sequences:

>Translated_488_residues
MTAIASEKLSKNASLVKDWAKGYESQLNEYSYWIDEVEGTIPPELQGTLFRNGAGSLEVNGQKIGHPFDGDGMICAITFE
QGRAHFQNRYVRTEGYLKEQAAGKILYRGFGTQKAGGWLANIFNTNFKNAANTSVIYWGDKLWAMWEGGHPHQLTPETLE
TIGPDTLDGLLTADQPFSAHPRIIDGRFINFGVKGIASQSLTIFELDEQGNPLKQSSHPLSGFAFLHDMLVTENYCIFVQ
HPFQVKGLPFLLGFKTIEQCFDFNPEQPTKIILISRHGNHDLEILETDSFFGFHHGNAWEKEGKLYFESVCSDFFPQKQQ
DELDFEQIDFESFPTGELWEFEVNLSSKKVVHRKVVERGCEFPSVHPQWVGKEHRYVYMSVCDSSDKNGPLQAILKLDKE
SGEQQIWSVAPRAFPGEPIFVPRPDSTQEDDGWLLSLVFDAATYRSYLAILDAQDLNTVIAKLHLQHHIPHGFHGSWTPN
VFRDSSKN
>Mature_487_residues
TAIASEKLSKNASLVKDWAKGYESQLNEYSYWIDEVEGTIPPELQGTLFRNGAGSLEVNGQKIGHPFDGDGMICAITFEQ
GRAHFQNRYVRTEGYLKEQAAGKILYRGFGTQKAGGWLANIFNTNFKNAANTSVIYWGDKLWAMWEGGHPHQLTPETLET
IGPDTLDGLLTADQPFSAHPRIIDGRFINFGVKGIASQSLTIFELDEQGNPLKQSSHPLSGFAFLHDMLVTENYCIFVQH
PFQVKGLPFLLGFKTIEQCFDFNPEQPTKIILISRHGNHDLEILETDSFFGFHHGNAWEKEGKLYFESVCSDFFPQKQQD
ELDFEQIDFESFPTGELWEFEVNLSSKKVVHRKVVERGCEFPSVHPQWVGKEHRYVYMSVCDSSDKNGPLQAILKLDKES
GEQQIWSVAPRAFPGEPIFVPRPDSTQEDDGWLLSLVFDAATYRSYLAILDAQDLNTVIAKLHLQHHIPHGFHGSWTPNV
FRDSSKN

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI74027270, Length=516, Percent_Identity=25, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI4506591, Length=506, Percent_Identity=26.0869565217391, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71998242, Length=514, Percent_Identity=24.5136186770428, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32564756, Length=512, Percent_Identity=24.21875, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24646669, Length=345, Percent_Identity=24.9275362318841, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 55269; Mature: 55137

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIASEKLSKNASLVKDWAKGYESQLNEYSYWIDEVEGTIPPELQGTLFRNGAGSLEVN
CCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEC
GQKIGHPFDGDGMICAITFEQGRAHFQNRYVRTEGYLKEQAAGKILYRGFGTQKAGGWLA
CCCCCCCCCCCCEEEEEEECCCHHHHHHCEEECCCCHHHHHCCEEEEECCCCCCCCCEEE
NIFNTNFKNAANTSVIYWGDKLWAMWEGGHPHQLTPETLETIGPDTLDGLLTADQPFSAH
EHHCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCHHHHHHCCCCHHHCCEECCCCCCCC
PRIIDGRFINFGVKGIASQSLTIFELDEQGNPLKQSSHPLSGFAFLHDMLVTENYCIFVQ
CEEECCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEE
HPFQVKGLPFLLGFKTIEQCFDFNPEQPTKIILISRHGNHDLEILETDSFFGFHHGNAWE
CCCEECCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEECCCEEEECCCCCCC
KEGKLYFESVCSDFFPQKQQDELDFEQIDFESFPTGELWEFEVNLSSKKVVHRKVVERGC
CCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCC
EFPSVHPQWVGKEHRYVYMSVCDSSDKNGPLQAILKLDKESGEQQIWSVAPRAFPGEPIF
CCCCCCHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEE
VPRPDSTQEDDGWLLSLVFDAATYRSYLAILDAQDLNTVIAKLHLQHHIPHGFHGSWTPN
EECCCCCCCCCCEEEEHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHCCCCCCCCCCCCC
VFRDSSKN
CCCCCCCC
>Mature Secondary Structure 
TAIASEKLSKNASLVKDWAKGYESQLNEYSYWIDEVEGTIPPELQGTLFRNGAGSLEVN
CCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEC
GQKIGHPFDGDGMICAITFEQGRAHFQNRYVRTEGYLKEQAAGKILYRGFGTQKAGGWLA
CCCCCCCCCCCCEEEEEEECCCHHHHHHCEEECCCCHHHHHCCEEEEECCCCCCCCCEEE
NIFNTNFKNAANTSVIYWGDKLWAMWEGGHPHQLTPETLETIGPDTLDGLLTADQPFSAH
EHHCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCHHHHHHCCCCHHHCCEECCCCCCCC
PRIIDGRFINFGVKGIASQSLTIFELDEQGNPLKQSSHPLSGFAFLHDMLVTENYCIFVQ
CEEECCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEE
HPFQVKGLPFLLGFKTIEQCFDFNPEQPTKIILISRHGNHDLEILETDSFFGFHHGNAWE
CCCEECCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEECCCEEEECCCCCCC
KEGKLYFESVCSDFFPQKQQDELDFEQIDFESFPTGELWEFEVNLSSKKVVHRKVVERGC
CCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCC
EFPSVHPQWVGKEHRYVYMSVCDSSDKNGPLQAILKLDKESGEQQIWSVAPRAFPGEPIF
CCCCCCHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEE
VPRPDSTQEDDGWLLSLVFDAATYRSYLAILDAQDLNTVIAKLHLQHHIPHGFHGSWTPN
EECCCCCCCCCCEEEEHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHCCCCCCCCCCCCC
VFRDSSKN
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]