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The map label for this gene is hisS

Identifier: 158333833

GI number: 158333833

Start: 628243

End: 629535

Strand: Reverse

Name: hisS

Synonym: AM1_0642

Alternate gene names: 158333833

Gene position: 629535-628243 (Counterclockwise)

Preceding gene: 158333835

Following gene: 158333832

Centisome position: 9.68

GC content: 50.89

Gene sequence:

>1293_bases
ATGGGATTTATTCAAGTTTCACGAGGGACACGGGACATTCTGCCAGATGAGGTGATCTATTGGCAGTATGTCGAAGCCAC
AGCCCGTCAGCTTCTCCATCAGGCGGCATACCGAGAGCTGCGGACCCCCATCTTTGAGCAAACTAATTTATTTGAACGGG
GTATCGGTGAAGCCACAGACGTCGTCGGCAAGGAAATGTACACTTTCCAAGATCGAGGCGATCGTTCGATTACCCTGCGT
CCAGAAGGGACGGCTGGCGCTGTCCGTTCATTTATCGAGAACAAACTTCATGCCCAGGGAGGCGTTCAGCGTCTGTGGTA
TATCGGACCCATGTTTCGCTATGAAAGACCCGGAGCAGGACGGCAACGCCAATTTCACCAAATCGGAGTAGAAGCGCTGG
GTAGCCAAGATCCACGTGCTGATGCAGAAGTTATTGCTATCGCCTCCCAACTCCTTAAATCGTTAGGCGTTCCTGATTGG
ACCTTATCTCTCAACTCCCTCGGCACGGCTGAAGATCGACAGAAATACCGTGAGGCTTTAGTCACTTACCTTAGTCAGTA
CAAAGACGACCTAGACCCTGACTCCCAGGATCGCCTCCAGCGTAATCCTCTGCGCATCTTAGATAGCAAGGATCCAAAAA
CAAAAGAAATAGCCCAGTCTGCCCCCAACATCTTGGATTACTTGGGCACTGACTCCAAACAGCATTTTGACCGTGTTCAG
CAACTTCTCACGGATCTAGATATTGCCTATAAGCTCAATCCTTGCCTAGTTAGAGGTCTAGATTACTACACCCACACCGC
CTTTGAGTTTGAGTTGGAGGGATTAGGGAATCAAGCCACCGTCTGTGGCGGAGGACGCTACGATCGCCTCGTTTCTGAAC
TGGGGGGCCCCGAAACCCCTGCAGTGGGGTGGGCAATCGGGATGGAGCGACTAATTCTGCTCCTGCAAAATGCTGAGATC
ACCTTAAATCAATCTTTAGATTTTTACTGCGTGGCTCGGGGACCAGAAGCAGAAGCTCAAGCCTTGCTAATTTGCCAAAA
TTTGAGGGAAAACGGCTTTTCCGTGGAGATGGATTTGAGTGGGAGTGCGTTTGGCAAACAGCTCAAACGAGCCAATCGGA
GCGGGGCTTTAGCCTGCTTAATTTTGGGCGATACAGAAGCTTGCGATCGCACCGTGCAACTCAAATGGCTAGCCTCTGGC
GAACAAGAATCCATAGCTCAGGCCGATCTCAGAAACCTCACGTCCCAACTCCAAAGTAAACTGACCGCAGCCAAAGGGAA
TTCCTCCACCTAA

Upstream 100 bases:

>100_bases
ACAACGCTGATTCCTTTTCAACCTCAAATAAATCTGAGGCAGAACTGAGCCAATCGGAGATAATAGGCACCGGGGTTAAG
GCCATAAGGATATTTTTTTC

Downstream 100 bases:

>100_bases
AGCTCCCATTTCTGAACAAGATCAGTTAGACTGTGCTGCATGTTGGAATCCCTTAACAACACCCAGAAAATATGGATCTC
TGAACTGTGGCTGTGTGGAG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MGFIQVSRGTRDILPDEVIYWQYVEATARQLLHQAAYRELRTPIFEQTNLFERGIGEATDVVGKEMYTFQDRGDRSITLR
PEGTAGAVRSFIENKLHAQGGVQRLWYIGPMFRYERPGAGRQRQFHQIGVEALGSQDPRADAEVIAIASQLLKSLGVPDW
TLSLNSLGTAEDRQKYREALVTYLSQYKDDLDPDSQDRLQRNPLRILDSKDPKTKEIAQSAPNILDYLGTDSKQHFDRVQ
QLLTDLDIAYKLNPCLVRGLDYYTHTAFEFELEGLGNQATVCGGGRYDRLVSELGGPETPAVGWAIGMERLILLLQNAEI
TLNQSLDFYCVARGPEAEAQALLICQNLRENGFSVEMDLSGSAFGKQLKRANRSGALACLILGDTEACDRTVQLKWLASG
EQESIAQADLRNLTSQLQSKLTAAKGNSST

Sequences:

>Translated_430_residues
MGFIQVSRGTRDILPDEVIYWQYVEATARQLLHQAAYRELRTPIFEQTNLFERGIGEATDVVGKEMYTFQDRGDRSITLR
PEGTAGAVRSFIENKLHAQGGVQRLWYIGPMFRYERPGAGRQRQFHQIGVEALGSQDPRADAEVIAIASQLLKSLGVPDW
TLSLNSLGTAEDRQKYREALVTYLSQYKDDLDPDSQDRLQRNPLRILDSKDPKTKEIAQSAPNILDYLGTDSKQHFDRVQ
QLLTDLDIAYKLNPCLVRGLDYYTHTAFEFELEGLGNQATVCGGGRYDRLVSELGGPETPAVGWAIGMERLILLLQNAEI
TLNQSLDFYCVARGPEAEAQALLICQNLRENGFSVEMDLSGSAFGKQLKRANRSGALACLILGDTEACDRTVQLKWLASG
EQESIAQADLRNLTSQLQSKLTAAKGNSST
>Mature_429_residues
GFIQVSRGTRDILPDEVIYWQYVEATARQLLHQAAYRELRTPIFEQTNLFERGIGEATDVVGKEMYTFQDRGDRSITLRP
EGTAGAVRSFIENKLHAQGGVQRLWYIGPMFRYERPGAGRQRQFHQIGVEALGSQDPRADAEVIAIASQLLKSLGVPDWT
LSLNSLGTAEDRQKYREALVTYLSQYKDDLDPDSQDRLQRNPLRILDSKDPKTKEIAQSAPNILDYLGTDSKQHFDRVQQ
LLTDLDIAYKLNPCLVRGLDYYTHTAFEFELEGLGNQATVCGGGRYDRLVSELGGPETPAVGWAIGMERLILLLQNAEIT
LNQSLDFYCVARGPEAEAQALLICQNLRENGFSVEMDLSGSAFGKQLKRANRSGALACLILGDTEACDRTVQLKWLASGE
QESIAQADLRNLTSQLQSKLTAAKGNSST

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=460, Percent_Identity=24.7826086956522, Blast_Score=93, Evalue=6e-19,
Organism=Escherichia coli, GI1788861, Length=419, Percent_Identity=48.6873508353222, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6325290, Length=503, Percent_Identity=23.0616302186879, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_ACAM1 (B0CDA0)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001515005.1
- ProteinModelPortal:   B0CDA0
- SMR:   B0CDA0
- GeneID:   5679470
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_0642
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   AMAR329726:AM1_0642-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47929; Mature: 47797

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFIQVSRGTRDILPDEVIYWQYVEATARQLLHQAAYRELRTPIFEQTNLFERGIGEATD
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
VVGKEMYTFQDRGDRSITLRPEGTAGAVRSFIENKLHAQGGVQRLWYIGPMFRYERPGAG
HHHHHHHEECCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHEECCCCCC
RQRQFHQIGVEALGSQDPRADAEVIAIASQLLKSLGVPDWTLSLNSLGTAEDRQKYREAL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHH
VTYLSQYKDDLDPDSQDRLQRNPLRILDSKDPKTKEIAQSAPNILDYLGTDSKQHFDRVQ
HHHHHHHHHCCCCCCHHHHHCCCCEEECCCCCCHHHHHHHCHHHHHHHCCCCHHHHHHHH
QLLTDLDIAYKLNPCLVRGLDYYTHTAFEFELEGLGNQATVCGGGRYDRLVSELGGPETP
HHHHHHHHHEECCHHHHHCCHHHHHCEEEEEECCCCCCEEEECCCHHHHHHHHCCCCCCC
AVGWAIGMERLILLLQNAEITLNQSLDFYCVARGPEAEAQALLICQNLRENGFSVEMDLS
CHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECC
GSAFGKQLKRANRSGALACLILGDTEACDRTVQLKWLASGEQESIAQADLRNLTSQLQSK
CHHHHHHHHHCCCCCCEEEEEECCCHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
LTAAKGNSST
HHHCCCCCCC
>Mature Secondary Structure 
GFIQVSRGTRDILPDEVIYWQYVEATARQLLHQAAYRELRTPIFEQTNLFERGIGEATD
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
VVGKEMYTFQDRGDRSITLRPEGTAGAVRSFIENKLHAQGGVQRLWYIGPMFRYERPGAG
HHHHHHHEECCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHEECCCCCC
RQRQFHQIGVEALGSQDPRADAEVIAIASQLLKSLGVPDWTLSLNSLGTAEDRQKYREAL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHH
VTYLSQYKDDLDPDSQDRLQRNPLRILDSKDPKTKEIAQSAPNILDYLGTDSKQHFDRVQ
HHHHHHHHHCCCCCCHHHHHCCCCEEECCCCCCHHHHHHHCHHHHHHHCCCCHHHHHHHH
QLLTDLDIAYKLNPCLVRGLDYYTHTAFEFELEGLGNQATVCGGGRYDRLVSELGGPETP
HHHHHHHHHEECCHHHHHCCHHHHHCEEEEEECCCCCCEEEECCCHHHHHHHHCCCCCCC
AVGWAIGMERLILLLQNAEITLNQSLDFYCVARGPEAEAQALLICQNLRENGFSVEMDLS
CHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECC
GSAFGKQLKRANRSGALACLILGDTEACDRTVQLKWLASGEQESIAQADLRNLTSQLQSK
CHHHHHHHHHCCCCCCEEEEEECCCHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
LTAAKGNSST
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA