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The map label for this gene is pgk

Identifier: 158333828

GI number: 158333828

Start: 623963

End: 625168

Strand: Direct

Name: pgk

Synonym: AM1_0637

Alternate gene names: 158333828

Gene position: 623963-625168 (Clockwise)

Preceding gene: 158333826

Following gene: 158333834

Centisome position: 9.59

GC content: 47.43

Gene sequence:

>1206_bases
GTGTCCAAGAAAACAATAGCCAATTTAACATCGGCAGATTTATCTGGTAAGCGAGTTTTGGTGCGTGCCGATTTCAATGT
GCCCTTAGATGGCGACGGCAAAATTACAGATGATACCCGCATTCGAGCTGCTCTCCCCACGATTCAAGACCTAACGTCTA
AAGGTGCTAAGGTCATTCTTGCGAGCCACTTTGGACGTCCTAAGGGACAAGTCAATGAGTCCATGCGCCTAACACCCGTT
GCTGCACGTTTGTCTGAATTGCTGGGTCAAGCGGTCACCAAATGTGATGACTGTATTGGAGATGAGGTGGCGGCAAAAGT
CGGCGCATTACAGAATGGACAAGTTGCCCTTCTGGAAAATGTACGTTTCCATTCGGGTGAAGAGGCTAATGACCCTGAGT
TTGCCCGCCAGCTAGCTTCAGTGGCTGACCTGTATGTCAATGATGCTTTTGGTACTGCCCACCGAGCCCATGCTTCTACT
GAAGGAGTCACGCATCACCTGAGCCCTTCTGTGGCGGGTTATTTAATTGAGAAAGAACTGAAGTATCTACAGGCTGCTAT
TGAGGGCCCTCAGCGTCCTTTGGCCGCTATCGTGGGTGGTTCAAAAGTCTCCAGCAAAATTGGTGTGATTGAAACCTTGT
TAGACAAGGTAGACAAGCTACTGATTGGTGGCGGTATGATTTTCACTTTCTATAAAGCTCGTGGTTTAGCTGTGGGTAAG
TCTTTAGTTGAAGAAGACAAGCTAGATTTAGCTCGTACTCTAGAGGCTAAGGCTAAAGAGAAGGGAGTCGCTCTGTTATT
GCCTAGTGATGTTGTGGTTGCAGATAACTTTGCTGCTGATGCCAATGCTCAGACTGTGAGCATCAATAATATTCCTGATG
GCTGGATGGGCTTGGATATTGGTCCCAACTCTGTGAAAGAGTTTCAGGCTGCCCTTTCTGATTGCAAAACTGTGATTTGG
AATGGACCGATGGGTGTGTTTGAATTCGATAAATTTGCAGCAGGAACTGATGCGGTTGCCCATAGCTTGGCTGCAATTAC
CGAGACAGGTGCTGACACCATTATTGGTGGAGGTGACTCGGTTGCGGCAGTTGAAAAAGTGGGTGTGGCTGAAAAAATGA
GCCATATTTCTACCGGAGGTGGTGCAAGTCTAGAGCTTTTGGAAGGTAAAGTCTTACCTGGAATTGCGGCCCTTGATGAA
GCTTAA

Upstream 100 bases:

>100_bases
TTGGGTATAGATTTATCCCAACCATCATCATGCCAATACCCCATGCGCTCTGCTATGGGGGGTAATAAAACGCAATAGAC
GCATTTAGGAGATTTTTACT

Downstream 100 bases:

>100_bases
GACAGTCATCTAAAGCTATAAAACATAGCGAGAAAGGGGGGCTCAAACGAGCCCCCCTTTCTTGTCAAAAACTAGGTCAA
TGAATCTGACTTTTCCCGCT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSKKTIANLTSADLSGKRVLVRADFNVPLDGDGKITDDTRIRAALPTIQDLTSKGAKVILASHFGRPKGQVNESMRLTPV
AARLSELLGQAVTKCDDCIGDEVAAKVGALQNGQVALLENVRFHSGEEANDPEFARQLASVADLYVNDAFGTAHRAHAST
EGVTHHLSPSVAGYLIEKELKYLQAAIEGPQRPLAAIVGGSKVSSKIGVIETLLDKVDKLLIGGGMIFTFYKARGLAVGK
SLVEEDKLDLARTLEAKAKEKGVALLLPSDVVVADNFAADANAQTVSINNIPDGWMGLDIGPNSVKEFQAALSDCKTVIW
NGPMGVFEFDKFAAGTDAVAHSLAAITETGADTIIGGGDSVAAVEKVGVAEKMSHISTGGGASLELLEGKVLPGIAALDE
A

Sequences:

>Translated_401_residues
MSKKTIANLTSADLSGKRVLVRADFNVPLDGDGKITDDTRIRAALPTIQDLTSKGAKVILASHFGRPKGQVNESMRLTPV
AARLSELLGQAVTKCDDCIGDEVAAKVGALQNGQVALLENVRFHSGEEANDPEFARQLASVADLYVNDAFGTAHRAHAST
EGVTHHLSPSVAGYLIEKELKYLQAAIEGPQRPLAAIVGGSKVSSKIGVIETLLDKVDKLLIGGGMIFTFYKARGLAVGK
SLVEEDKLDLARTLEAKAKEKGVALLLPSDVVVADNFAADANAQTVSINNIPDGWMGLDIGPNSVKEFQAALSDCKTVIW
NGPMGVFEFDKFAAGTDAVAHSLAAITETGADTIIGGGDSVAAVEKVGVAEKMSHISTGGGASLELLEGKVLPGIAALDE
A
>Mature_400_residues
SKKTIANLTSADLSGKRVLVRADFNVPLDGDGKITDDTRIRAALPTIQDLTSKGAKVILASHFGRPKGQVNESMRLTPVA
ARLSELLGQAVTKCDDCIGDEVAAKVGALQNGQVALLENVRFHSGEEANDPEFARQLASVADLYVNDAFGTAHRAHASTE
GVTHHLSPSVAGYLIEKELKYLQAAIEGPQRPLAAIVGGSKVSSKIGVIETLLDKVDKLLIGGGMIFTFYKARGLAVGKS
LVEEDKLDLARTLEAKAKEKGVALLLPSDVVVADNFAADANAQTVSINNIPDGWMGLDIGPNSVKEFQAALSDCKTVIWN
GPMGVFEFDKFAAGTDAVAHSLAAITETGADTIIGGGDSVAAVEKVGVAEKMSHISTGGGASLELLEGKVLPGIAALDEA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=417, Percent_Identity=45.3237410071942, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI31543397, Length=420, Percent_Identity=44.5238095238095, Blast_Score=336, Evalue=2e-92,
Organism=Escherichia coli, GI1789294, Length=398, Percent_Identity=45.4773869346734, Blast_Score=320, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17508823, Length=407, Percent_Identity=50.8599508599509, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319857, Length=417, Percent_Identity=49.8800959232614, Blast_Score=381, Evalue=1e-107,
Organism=Drosophila melanogaster, GI17136394, Length=419, Percent_Identity=48.2100238663485, Blast_Score=348, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24581203, Length=413, Percent_Identity=44.5520581113801, Blast_Score=335, Evalue=4e-92,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_ACAM1 (B0CD95)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001515000.1
- ProteinModelPortal:   B0CD95
- SMR:   B0CD95
- GeneID:   5679465
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_0637
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   AMAR329726:AM1_0637-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41862; Mature: 41731

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 40-40 BINDING 122-122 BINDING 155-155 BINDING 206-206 BINDING 297-297 BINDING 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKTIANLTSADLSGKRVLVRADFNVPLDGDGKITDDTRIRAALPTIQDLTSKGAKVIL
CCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHCCCCEEEE
ASHFGRPKGQVNESMRLTPVAARLSELLGQAVTKCDDCIGDEVAAKVGALQNGQVALLEN
EECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEC
VRFHSGEEANDPEFARQLASVADLYVNDAFGTAHRAHASTEGVTHHLSPSVAGYLIEKEL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEECCCHHHHHHHHHHHH
KYLQAAIEGPQRPLAAIVGGSKVSSKIGVIETLLDKVDKLLIGGGMIFTFYKARGLAVGK
HHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
SLVEEDKLDLARTLEAKAKEKGVALLLPSDVVVADNFAADANAQTVSINNIPDGWMGLDI
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCCCCEEEECCCCCCCEECCC
GPNSVKEFQAALSDCKTVIWNGPMGVFEFDKFAAGTDAVAHSLAAITETGADTIIGGGDS
CCHHHHHHHHHHHHCCEEEECCCCCCEEHHHHCCCHHHHHHHHHHHHHCCCCCEECCCCC
VAAVEKVGVAEKMSHISTGGGASLELLEGKVLPGIAALDEA
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHCCCC
>Mature Secondary Structure 
SKKTIANLTSADLSGKRVLVRADFNVPLDGDGKITDDTRIRAALPTIQDLTSKGAKVIL
CCCHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHCCCCEEEE
ASHFGRPKGQVNESMRLTPVAARLSELLGQAVTKCDDCIGDEVAAKVGALQNGQVALLEN
EECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEC
VRFHSGEEANDPEFARQLASVADLYVNDAFGTAHRAHASTEGVTHHLSPSVAGYLIEKEL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEECCCHHHHHHHHHHHH
KYLQAAIEGPQRPLAAIVGGSKVSSKIGVIETLLDKVDKLLIGGGMIFTFYKARGLAVGK
HHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
SLVEEDKLDLARTLEAKAKEKGVALLLPSDVVVADNFAADANAQTVSINNIPDGWMGLDI
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCCCCEEEECCCCCCCEECCC
GPNSVKEFQAALSDCKTVIWNGPMGVFEFDKFAAGTDAVAHSLAAITETGADTIIGGGDS
CCHHHHHHHHHHHHCCEEEECCCCCCEEHHHHCCCHHHHHHHHHHHHHCCCCCEECCCCC
VAAVEKVGVAEKMSHISTGGGASLELLEGKVLPGIAALDEA
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA