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The map label for this gene is birA [H]

Identifier: 158333816

GI number: 158333816

Start: 613526

End: 614221

Strand: Reverse

Name: birA [H]

Synonym: AM1_0625

Alternate gene names: 158333816

Gene position: 614221-613526 (Counterclockwise)

Preceding gene: 158333820

Following gene: 158333815

Centisome position: 9.44

GC content: 50.14

Gene sequence:

>696_bases
GTGAGTTTTGATCAACCCGCGTTTCAGCAGTATCTAACCACCCAAACTTGGGGACGGCCGCTCCACCACTTTGAGGTCCT
TGACTCTACCAACACAAAACTCTGGGAGTTAATTAAGCAAGGGGCGAAACCGGGAACGGCGGTTGTTGCCGCCCAACAGC
GAGCCGGTCGGGGCCAGCGGGGGCGGACTTGGGAGTCTTCTCTGGGGGGACTGTATCTGTCGGTTGCGATCGCACCTTGT
ATTCCTGCCGATCAAACTGCCCAACTCACCGTGGCAACCATTTGGGGCCTGGCTACCCTGCTAAGACAGCATCACATCCC
AGTGTGGCTTAAGTGGCCTAATGATCTACTTGTACTTGGGAAAAAATTTGGGGGCATCCTGATCGAAACTAGTCTCCAAA
ATCAACACGTTCAGACAGCAGTGGTCGGCATTGGCCTGAATTGGCGTAATCCAGTGCCAGATCAAGCCATTAACCTAGCG
ACCATTACAGAAAGGTCCCCCGCCCACCATAGATCTTCGATTCATTCCTTAGAAGAATTAACAGCCCTCACCTTATTGGG
TCTAGAAAATGGCTATCTATTTTGGCAAACCCAAGGCATCCATCCATTACTACCCGATTATCTAGATCTAATTAAAATTT
ATCCTCAAGCCAGTCAACCCATCCGCCAAGATTTGGCAAAACGTCTAGGTTTATGA

Upstream 100 bases:

>100_bases
AATTCCAGGTATGCATGGGGGCAGAATAGACCTTAAACAATGCTAGGCTTACCTATGCTAGCGCGCCGATTCCAGTTTTA
ACGGGCCTAGCCCTCGAACT

Downstream 100 bases:

>100_bases
ACTCATCAACACTCTAGGTATAGTAATAATGAAGAAAGTCGTTTCCTGAAAATTTATCTACCATTAGGAAATAAGTGACT
AGGTTGATTCTTCCTTAGTA

Product: biotin-[acetyl-CoA-carboxylase] ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSFDQPAFQQYLTTQTWGRPLHHFEVLDSTNTKLWELIKQGAKPGTAVVAAQQRAGRGQRGRTWESSLGGLYLSVAIAPC
IPADQTAQLTVATIWGLATLLRQHHIPVWLKWPNDLLVLGKKFGGILIETSLQNQHVQTAVVGIGLNWRNPVPDQAINLA
TITERSPAHHRSSIHSLEELTALTLLGLENGYLFWQTQGIHPLLPDYLDLIKIYPQASQPIRQDLAKRLGL

Sequences:

>Translated_231_residues
MSFDQPAFQQYLTTQTWGRPLHHFEVLDSTNTKLWELIKQGAKPGTAVVAAQQRAGRGQRGRTWESSLGGLYLSVAIAPC
IPADQTAQLTVATIWGLATLLRQHHIPVWLKWPNDLLVLGKKFGGILIETSLQNQHVQTAVVGIGLNWRNPVPDQAINLA
TITERSPAHHRSSIHSLEELTALTLLGLENGYLFWQTQGIHPLLPDYLDLIKIYPQASQPIRQDLAKRLGL
>Mature_230_residues
SFDQPAFQQYLTTQTWGRPLHHFEVLDSTNTKLWELIKQGAKPGTAVVAAQQRAGRGQRGRTWESSLGGLYLSVAIAPCI
PADQTAQLTVATIWGLATLLRQHHIPVWLKWPNDLLVLGKKFGGILIETSLQNQHVQTAVVGIGLNWRNPVPDQAINLAT
ITERSPAHHRSSIHSLEELTALTLLGLENGYLFWQTQGIHPLLPDYLDLIKIYPQASQPIRQDLAKRLGL

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=184, Percent_Identity=30.9782608695652, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 25737; Mature: 25606

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDQPAFQQYLTTQTWGRPLHHFEVLDSTNTKLWELIKQGAKPGTAVVAAQQRAGRGQR
CCCCCHHHHHHHHHHHCCCCCHHHEECCCCCHHHHHHHHHCCCCCCEEEEEHHHCCCCCC
GRTWESSLGGLYLSVAIAPCIPADQTAQLTVATIWGLATLLRQHHIPVWLKWPNDLLVLG
CCCHHHHHCCEEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEE
KKFGGILIETSLQNQHVQTAVVGIGLNWRNPVPDQAINLATITERSPAHHRSSIHSLEEL
CCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCHHHEEEEEECCCCCHHHHHHHHHHHHH
TALTLLGLENGYLFWQTQGIHPLLPDYLDLIKIYPQASQPIRQDLAKRLGL
HHHHEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SFDQPAFQQYLTTQTWGRPLHHFEVLDSTNTKLWELIKQGAKPGTAVVAAQQRAGRGQR
CCCCHHHHHHHHHHHCCCCCHHHEECCCCCHHHHHHHHHCCCCCCEEEEEHHHCCCCCC
GRTWESSLGGLYLSVAIAPCIPADQTAQLTVATIWGLATLLRQHHIPVWLKWPNDLLVLG
CCCHHHHHCCEEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEE
KKFGGILIETSLQNQHVQTAVVGIGLNWRNPVPDQAINLATITERSPAHHRSSIHSLEEL
CCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCHHHEEEEEECCCCCHHHHHHHHHHHHH
TALTLLGLENGYLFWQTQGIHPLLPDYLDLIKIYPQASQPIRQDLAKRLGL
HHHHEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]