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The map label for this gene is ribAB [H]

Identifier: 158333800

GI number: 158333800

Start: 598330

End: 600012

Strand: Reverse

Name: ribAB [H]

Synonym: AM1_0608

Alternate gene names: 158333800

Gene position: 600012-598330 (Counterclockwise)

Preceding gene: 158333803

Following gene: 158333799

Centisome position: 9.23

GC content: 47.3

Gene sequence:

>1683_bases
TTGAATCCCCACGCTACGCCAACAGACTCTTTTGAATTTGACTCCATTGAAGATGCCCTTGCGGAGTTTAAAGCGGGACA
TGCCATTGTCGTAGTGGATGATGAGAACCGGGAAAACGAAGGCGATCTAATTTGTGCAGCTCAATTCGCCACCCCTGACA
TGATTAACTTTATGGCCGTGGAAGCGCGGGGGTTGATTTGCCTAGCCATGACTGGGGAGCGATTAGACGCATTAGAGCTG
CCACTGATGGTTACCAATAACACCGACATGAACCAAACGGCCTTTACCGTCAGTATCGATGCCTCTCCTCATCTAGGCGT
AACAACCGGCATTTCAGCGGAAGACCGAGCCCGTACGATTCAAGTCGCCGTCAATCCAGATTCCTTACCCACAGATTTGC
GACGTCCTGGGCACATTTTCCCTTTGAGAGCCCGCTCTGGTGGAGTGTTACAGCGAGCAGGTCATACTGAATCGGCGATT
GATATGACTCGCTTAGCAGGGTTATATCCAGCTGGCGTAATTTGTGAAATCCAAAATCCCGATGGATCCATGGCTCGCCT
ACCGCAACTCAAAGACTATGCCCATGAACATCAGCTTAAAATCATCAGCATTGCCGATTTAATTAGCTATCGGCTTCAAC
ATGAGCGGTTTGTTCATCGAGAAACTGTTGCTGATATGCCCACTGAGTTTGGCCAGTTTCAGATTTATGCTTACCGCAAT
CAGCTCGATCACAAAGAACATGTCGCCATTGTTAAAGGCGATCCAAAGCAGTTTGGTCAACAGCCCGTGATGGTGCGGAT
GCACTCCGAATGCCTCACCGGAGATGCCATCGGCTCCCTTCGGTGTGACTGTCGTATGCAGCTCCAGGCCGCTCTCAAAA
TGATTGAGAATGTGGGGCAGGGCGTCGTCGTGTATTTAAGACAAGAAGGCCGCGGGATTGGTCTAGTCAATAAACTGAAG
GCCTATTCCTTGCAAGATATGGGTCTCGATACCGTAGAAGCAAATGAAAAGTTAGGATTTCCAGCCGATTTACGAAACTA
CGGTGTAGGGGCTCAAATCCTTAATGATCTGGGAATTCAAAAAATTCGCCTGATTACCAATAACCCCCGTAAGATAGCAG
GGCTCAAAGGCTATGGGTTGGAAGTTGCTGATCGAGTCCCCTTGCTAATCGAGGCCAACACCTACAATACGACCTACTTA
AACACTAAGGCAGAAAAGCTAGGTCATATGCTGTTGCAAACCTACCTGATGACGATTGCTATTCGATGGCAGGATGAACC
CTTAGATGTGACTCAGCGCTACGACCGTCTCGAAAAACTTCGTCATTTGGCAGACAACCAGCATCTTTTACTGCAAGAAG
AGGCCCGTCCTGTAGCAATAGCGCTATTCGAAGGCCCCTCCTTGATTTGTCATTTAGGTTTAGACCAGCCAGATGTTGCC
TCGGCAGATTGGTATCAACAAAAAGGACACCCTTACCGGGAAGCGGTTACTCGTATCTTTGGAGATTTAAAGCAGGATTC
TAATGTTGCTAGTGTTGAATTTTTGATTTCTTCTGGCACCGATCCGCTCAAAAATCTCCAAGTCAAATTAGACCGAAAAA
GCATTAAGGCAGATCAATTACCTTTACCCTTATGTCATGATTTAGAAACACAAACAATCTATAGCGTTGACCTAAGCGAA
TAG

Upstream 100 bases:

>100_bases
TTCGACTGGTTTTGGATAATCAAAGGATTCTGTGCGAACTGGCGTCCCCACCCCTTAGAATTAAGGGGAAGATGTTAAAA
AACGTTACGGTACCGTCACT

Downstream 100 bases:

>100_bases
CTATTTCTATATAGGAACATACCTATATAACCTGAGTTCAGGATAATCTTAAACCTTCATTCTTGCGTGGAAGAATCTAA
AAAATGGTCTTAACCTGAAC

Product: bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II/unknown domain fusion protein

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MNPHATPTDSFEFDSIEDALAEFKAGHAIVVVDDENRENEGDLICAAQFATPDMINFMAVEARGLICLAMTGERLDALEL
PLMVTNNTDMNQTAFTVSIDASPHLGVTTGISAEDRARTIQVAVNPDSLPTDLRRPGHIFPLRARSGGVLQRAGHTESAI
DMTRLAGLYPAGVICEIQNPDGSMARLPQLKDYAHEHQLKIISIADLISYRLQHERFVHRETVADMPTEFGQFQIYAYRN
QLDHKEHVAIVKGDPKQFGQQPVMVRMHSECLTGDAIGSLRCDCRMQLQAALKMIENVGQGVVVYLRQEGRGIGLVNKLK
AYSLQDMGLDTVEANEKLGFPADLRNYGVGAQILNDLGIQKIRLITNNPRKIAGLKGYGLEVADRVPLLIEANTYNTTYL
NTKAEKLGHMLLQTYLMTIAIRWQDEPLDVTQRYDRLEKLRHLADNQHLLLQEEARPVAIALFEGPSLICHLGLDQPDVA
SADWYQQKGHPYREAVTRIFGDLKQDSNVASVEFLISSGTDPLKNLQVKLDRKSIKADQLPLPLCHDLETQTIYSVDLSE

Sequences:

>Translated_560_residues
MNPHATPTDSFEFDSIEDALAEFKAGHAIVVVDDENRENEGDLICAAQFATPDMINFMAVEARGLICLAMTGERLDALEL
PLMVTNNTDMNQTAFTVSIDASPHLGVTTGISAEDRARTIQVAVNPDSLPTDLRRPGHIFPLRARSGGVLQRAGHTESAI
DMTRLAGLYPAGVICEIQNPDGSMARLPQLKDYAHEHQLKIISIADLISYRLQHERFVHRETVADMPTEFGQFQIYAYRN
QLDHKEHVAIVKGDPKQFGQQPVMVRMHSECLTGDAIGSLRCDCRMQLQAALKMIENVGQGVVVYLRQEGRGIGLVNKLK
AYSLQDMGLDTVEANEKLGFPADLRNYGVGAQILNDLGIQKIRLITNNPRKIAGLKGYGLEVADRVPLLIEANTYNTTYL
NTKAEKLGHMLLQTYLMTIAIRWQDEPLDVTQRYDRLEKLRHLADNQHLLLQEEARPVAIALFEGPSLICHLGLDQPDVA
SADWYQQKGHPYREAVTRIFGDLKQDSNVASVEFLISSGTDPLKNLQVKLDRKSIKADQLPLPLCHDLETQTIYSVDLSE
>Mature_560_residues
MNPHATPTDSFEFDSIEDALAEFKAGHAIVVVDDENRENEGDLICAAQFATPDMINFMAVEARGLICLAMTGERLDALEL
PLMVTNNTDMNQTAFTVSIDASPHLGVTTGISAEDRARTIQVAVNPDSLPTDLRRPGHIFPLRARSGGVLQRAGHTESAI
DMTRLAGLYPAGVICEIQNPDGSMARLPQLKDYAHEHQLKIISIADLISYRLQHERFVHRETVADMPTEFGQFQIYAYRN
QLDHKEHVAIVKGDPKQFGQQPVMVRMHSECLTGDAIGSLRCDCRMQLQAALKMIENVGQGVVVYLRQEGRGIGLVNKLK
AYSLQDMGLDTVEANEKLGFPADLRNYGVGAQILNDLGIQKIRLITNNPRKIAGLKGYGLEVADRVPLLIEANTYNTTYL
NTKAEKLGHMLLQTYLMTIAIRWQDEPLDVTQRYDRLEKLRHLADNQHLLLQEEARPVAIALFEGPSLICHLGLDQPDVA
SADWYQQKGHPYREAVTRIFGDLKQDSNVASVEFLISSGTDPLKNLQVKLDRKSIKADQLPLPLCHDLETQTIYSVDLSE

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate [H]

COG id: COG0807

COG function: function code H; GTP cyclohydrolase II

Gene ontology:
GO:0000166: Riboflavin biosynthesis protein ribAB (3,4-dihydroxy-2-butanone 4-phosphate synthase and GTP cyclohydrolase II)
GO:0000287: Riboflavin biosynthesis protein ribAB (3,4-dihydroxy-2-butanone 4-phosphate synthase and GTP cyclohydrolase II)
GO:0003824: Riboflavin biosynthesis protein ribAB (3,4-dihydroxy-2-butanone 4-phosphate synthase and GTP cyclohydrolase II)
GO:0003935: Riboflavin biosynthesis protein ribAB (3,4-dihydroxy-2-butanone 4-phosphate synthase and GTP cyclohydrolase II)
GO:0005525: Riboflavin biosynthesis protein ribAB (3,4-dihydroxy-2-butanone 4-phosphate synthase and GTP cyclohydrolase II)
GO:0008152: Riboflavin biosynthesis protein ribAB (3,4-dihydroxy-2-butanone 4-phosphate synthase and GTP cyclohydrolase II)
GO:0008270: Riboflavin biosynthesis protein ribAB (3,4-dihydroxy-2-butanone 4-phosphate synthase and GTP cyclohydrolase II)
GO:0008686: Riboflavin biosynthesis protein ribAB (3,4-dihydroxy-2-butanone 4-phosphate synthase and GTP cyclohydrolase II)
GO:0009231: Riboflavin biosynthesis protein ribAB (3,4-dihydroxy-2-butanone 4-phosphate synthase and GTP cyclohydrolase II)
GO:0016787: Riboflavin biosynthesis protein ribAB (3,4-dihydroxy-2-butanone 4-phosphate synthase and GTP cyclohydrolase II)
GO:0016829: Riboflavin biosynthesis protein ribAB (3,4-dihydroxy-2-butanone 4-phosphate synthase and GTP cyclohydrolase II)
GO:0030145: Riboflavin biosynthesis protein ribAB (3,4-dihydroxy-2-butanone 4-phosphate synthase and GTP cyclohydrolase II)
GO:0046872: Riboflavin biosynthesis protein ribAB (3,4-dihydroxy-2-butanone 4-phosphate synthase and GTP cyclohydrolase II)

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1789420, Length=204, Percent_Identity=48.0392156862745, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1787533, Length=192, Percent_Identity=50, Blast_Score=198, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6320695, Length=206, Percent_Identity=41.747572815534, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319438, Length=172, Percent_Identity=45.3488372093023, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12; =3.5.4.25 [H]

Molecular weight: Translated: 62379; Mature: 62379

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPHATPTDSFEFDSIEDALAEFKAGHAIVVVDDENRENEGDLICAAQFATPDMINFMAV
CCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCHHHEEEE
EARGLICLAMTGERLDALELPLMVTNNTDMNQTAFTVSIDASPHLGVTTGISAEDRARTI
ECCCEEEEEECCCCCCCEECCEEEECCCCCCCEEEEEEECCCCCCCEEECCCCCCCCEEE
QVAVNPDSLPTDLRRPGHIFPLRARSGGVLQRAGHTESAIDMTRLAGLYPAGVICEIQNP
EEEECCCCCCHHHCCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHCCCCCCEEEEEECC
DGSMARLPQLKDYAHEHQLKIISIADLISYRLQHERFVHRETVADMPTEFGQFQIYAYRN
CCCHHHCCCHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
QLDHKEHVAIVKGDPKQFGQQPVMVRMHSECLTGDAIGSLRCDCRMQLQAALKMIENVGQ
CCCCCCCEEEEECCHHHHCCCCEEEEECCHHCCCCCCCCEECCHHHHHHHHHHHHHHCCC
GVVVYLRQEGRGIGLVNKLKAYSLQDMGLDTVEANEKLGFPADLRNYGVGAQILNDLGIQ
CEEEEEEECCCCCCHHHHHHHCCHHHCCCCEECCCCCCCCCHHHHHCCCHHHHHHHCCCE
KIRLITNNPRKIAGLKGYGLEVADRVPLLIEANTYNTTYLNTKAEKLGHMLLQTYLMTIA
EEEEEECCCCEEECCCCCCCEECCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHEE
IRWQDEPLDVTQRYDRLEKLRHLADNQHLLLQEEARPVAIALFEGPSLICHLGLDQPDVA
EEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCEEEEECCCCCCCC
SADWYQQKGHPYREAVTRIFGDLKQDSNVASVEFLISSGTDPLKNLQVKLDRKSIKADQL
CCCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHCCEEEEEHHHCCCCCC
PLPLCHDLETQTIYSVDLSE
CCCHHCCCCCCEEEEEECCC
>Mature Secondary Structure
MNPHATPTDSFEFDSIEDALAEFKAGHAIVVVDDENRENEGDLICAAQFATPDMINFMAV
CCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCHHHEEEE
EARGLICLAMTGERLDALELPLMVTNNTDMNQTAFTVSIDASPHLGVTTGISAEDRARTI
ECCCEEEEEECCCCCCCEECCEEEECCCCCCCEEEEEEECCCCCCCEEECCCCCCCCEEE
QVAVNPDSLPTDLRRPGHIFPLRARSGGVLQRAGHTESAIDMTRLAGLYPAGVICEIQNP
EEEECCCCCCHHHCCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHCCCCCCEEEEEECC
DGSMARLPQLKDYAHEHQLKIISIADLISYRLQHERFVHRETVADMPTEFGQFQIYAYRN
CCCHHHCCCHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
QLDHKEHVAIVKGDPKQFGQQPVMVRMHSECLTGDAIGSLRCDCRMQLQAALKMIENVGQ
CCCCCCCEEEEECCHHHHCCCCEEEEECCHHCCCCCCCCEECCHHHHHHHHHHHHHHCCC
GVVVYLRQEGRGIGLVNKLKAYSLQDMGLDTVEANEKLGFPADLRNYGVGAQILNDLGIQ
CEEEEEEECCCCCCHHHHHHHCCHHHCCCCEECCCCCCCCCHHHHHCCCHHHHHHHCCCE
KIRLITNNPRKIAGLKGYGLEVADRVPLLIEANTYNTTYLNTKAEKLGHMLLQTYLMTIA
EEEEEECCCCEEECCCCCCCEECCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHEE
IRWQDEPLDVTQRYDRLEKLRHLADNQHLLLQEEARPVAIALFEGPSLICHLGLDQPDVA
EEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCEEEEECCCCCCCC
SADWYQQKGHPYREAVTRIFGDLKQDSNVASVEFLISSGTDPLKNLQVKLDRKSIKADQL
CCCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHCCEEEEEHHHCCCCCC
PLPLCHDLETQTIYSVDLSE
CCCHHCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]