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The map label for this gene is moeB [H]

Identifier: 158333708

GI number: 158333708

Start: 487521

End: 488693

Strand: Direct

Name: moeB [H]

Synonym: AM1_0514

Alternate gene names: 158333708

Gene position: 487521-488693 (Clockwise)

Preceding gene: 158333707

Following gene: 158333712

Centisome position: 7.5

GC content: 50.21

Gene sequence:

>1173_bases
ATGCTTAATCCGAATTTGGACCAGATTCAGCTCAATAAAGAAGAATATGAGCGGTTCTCTCGCCACCTCATTTTGCCGGA
GGTGGGCCTAGAGGGACAAAAACGCTTAAAAGCCGCTAGCATCCTTTGTGTTGGTACCGGAGGCTTAGGGTCACCGTTAT
TACTCTATTTGGCCGCCGCTGGAGTTGGCCGTTTGGGCATTGTCGATGCCGATGTGGTCGATAGCTCTAACCTCCAACGC
CAGGTGATTCACGGTACCTCTTGGGTGGGCAAGCCTAAGATCGAATCCGCTAAAAATCGAATTCATGAGATCAACCCGCT
CTGTCAGGTGGACCTGTATAACACCTGGCTCACCTCAGAGAACGCCCTAGAAATTGCTGAACCTTATGACATTGTTATTG
ACGGTACGGACAATTTCCCGACTCGGTATCTGGTCAACGATGCCTGCGTCTTGTTAGATAAGCCCAATGTGTATGGGTCG
ATCTACCGCTTTGAAGGCCAAGCCACTGTATTTAATTACGAGGGTGGTCCCAACTATCGGGATTTGTATCCAGAACCCCC
ACCTCCTGGACTGGTGCCCTCTTGTGCTGAAGGGGGTGTTCTAGGGATATTGCCAGGTATTATTGGTATTATTCAAGCCA
CCGAAGCGATTAAGATTATTTTGGGCCAGGGCACAACGTTAAGTGGCCGTTTACTGCTCTTTAACTCCCTTGACATGAAG
TTTCGAGAGTTAAAGCTCAGACCCAACCCAGAGCGTCCTGTGATCGAAAAGCTGGTGGATTACCAAGAGTTCTGTGGTAT
TCCTCAAGCCCAAGCAGCCGAAGCGGAGCAAAAAGCCAATATGGAAGAAATGACCGTTCAAGATCTCAAGCAACTGCTCG
ATAGCGGTGCGGATGATTTTGTCCTCTTGGATGTGCGTAATCCCCATGAATATGAGATTGCCCGCATCCCTGGCTCAGTC
TTGATCCCCTTGTCTGAGATTGAGAGTGGCGAAGCCATTCCCAAAGTCAAAGAGCTGCTGAATGGCCACCGCCTGATTGC
TCACTGCAAGATGGGGGGACGCTCGGCTAAGGCCCTGGGCTTCCTCCAAGAAGCAGGGATTTCAGGCACCAATGTGACCG
GCGGAATCACGGCCTGGAGCCGTGAAATCGATTCTTCTGTACCTGAATACTAA

Upstream 100 bases:

>100_bases
TTCGAGCAGAACCAGTAACGGTTGTGGATGATGTGGTCCCGTCAACCCTTGAAACAGATATTTAGAAACCTCATACATCT
TTCCTTTAAGGATGTCCGCC

Downstream 100 bases:

>100_bases
GCCCTGAATTAATAGGTATAGATGAATTTAATTTCAGGTGCGATATGCCCCAACCTATACTGCTAGCGCTGTTGTAGGTT
GGTGGCTAGGATCGCAGCTT

Product: hypothetical protein

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MLNPNLDQIQLNKEEYERFSRHLILPEVGLEGQKRLKAASILCVGTGGLGSPLLLYLAAAGVGRLGIVDADVVDSSNLQR
QVIHGTSWVGKPKIESAKNRIHEINPLCQVDLYNTWLTSENALEIAEPYDIVIDGTDNFPTRYLVNDACVLLDKPNVYGS
IYRFEGQATVFNYEGGPNYRDLYPEPPPPGLVPSCAEGGVLGILPGIIGIIQATEAIKIILGQGTTLSGRLLLFNSLDMK
FRELKLRPNPERPVIEKLVDYQEFCGIPQAQAAEAEQKANMEEMTVQDLKQLLDSGADDFVLLDVRNPHEYEIARIPGSV
LIPLSEIESGEAIPKVKELLNGHRLIAHCKMGGRSAKALGFLQEAGISGTNVTGGITAWSREIDSSVPEY

Sequences:

>Translated_390_residues
MLNPNLDQIQLNKEEYERFSRHLILPEVGLEGQKRLKAASILCVGTGGLGSPLLLYLAAAGVGRLGIVDADVVDSSNLQR
QVIHGTSWVGKPKIESAKNRIHEINPLCQVDLYNTWLTSENALEIAEPYDIVIDGTDNFPTRYLVNDACVLLDKPNVYGS
IYRFEGQATVFNYEGGPNYRDLYPEPPPPGLVPSCAEGGVLGILPGIIGIIQATEAIKIILGQGTTLSGRLLLFNSLDMK
FRELKLRPNPERPVIEKLVDYQEFCGIPQAQAAEAEQKANMEEMTVQDLKQLLDSGADDFVLLDVRNPHEYEIARIPGSV
LIPLSEIESGEAIPKVKELLNGHRLIAHCKMGGRSAKALGFLQEAGISGTNVTGGITAWSREIDSSVPEY
>Mature_390_residues
MLNPNLDQIQLNKEEYERFSRHLILPEVGLEGQKRLKAASILCVGTGGLGSPLLLYLAAAGVGRLGIVDADVVDSSNLQR
QVIHGTSWVGKPKIESAKNRIHEINPLCQVDLYNTWLTSENALEIAEPYDIVIDGTDNFPTRYLVNDACVLLDKPNVYGS
IYRFEGQATVFNYEGGPNYRDLYPEPPPPGLVPSCAEGGVLGILPGIIGIIQATEAIKIILGQGTTLSGRLLLFNSLDMK
FRELKLRPNPERPVIEKLVDYQEFCGIPQAQAAEAEQKANMEEMTVQDLKQLLDSGADDFVLLDVRNPHEYEIARIPGSV
LIPLSEIESGEAIPKVKELLNGHRLIAHCKMGGRSAKALGFLQEAGISGTNVTGGITAWSREIDSSVPEY

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:
GO:0003824: Molybdopterin biosynthesis protein MoeB, putative
GO:0005488: Molybdopterin biosynthesis protein MoeB, putative
GO:0008152: Molybdopterin biosynthesis protein MoeB, putative

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=407, Percent_Identity=43.7346437346437, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI4885649, Length=157, Percent_Identity=27.3885350318471, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787048, Length=242, Percent_Identity=40.495867768595, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87082356, Length=246, Percent_Identity=38.2113821138211, Blast_Score=145, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17540406, Length=400, Percent_Identity=38, Blast_Score=244, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI193203301, Length=169, Percent_Identity=26.6272189349112, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71984476, Length=118, Percent_Identity=35.5932203389831, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=412, Percent_Identity=37.621359223301, Blast_Score=233, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6320598, Length=140, Percent_Identity=30.7142857142857, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582879, Length=401, Percent_Identity=40.6483790523691, Blast_Score=297, Evalue=9e-81,
Organism=Drosophila melanogaster, GI24660640, Length=136, Percent_Identity=30.8823529411765, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 42654; Mature: 42654

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNPNLDQIQLNKEEYERFSRHLILPEVGLEGQKRLKAASILCVGTGGLGSPLLLYLAAA
CCCCCCCEEEECHHHHHHHHHEEECCCCCCCHHHHHCEEEEEEEECCCCCHHHHHHHHHC
GVGRLGIVDADVVDSSNLQRQVIHGTSWVGKPKIESAKNRIHEINPLCQVDLYNTWLTSE
CCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEEEHHEEECCC
NALEIAEPYDIVIDGTDNFPTRYLVNDACVLLDKPNVYGSIYRFEGQATVFNYEGGPNYR
CCEEECCCEEEEEECCCCCCCEEEECCEEEEEECCCCCEEEEEECCCEEEEEECCCCCCC
DLYPEPPPPGLVPSCAEGGVLGILPGIIGIIQATEAIKIILGQGTTLSGRLLLFNSLDMK
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCCCCEEEEEECCCCE
FRELKLRPNPERPVIEKLVDYQEFCGIPQAQAAEAEQKANMEEMTVQDLKQLLDSGADDF
EEEEECCCCCCCHHHHHHHCHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCE
VLLDVRNPHEYEIARIPGSVLIPLSEIESGEAIPKVKELLNGHRLIAHCKMGGRSAKALG
EEEEECCCCCEEEEECCCCEEEEHHHCCCCCCCHHHHHHHCCCEEEEEECCCCCHHHHHH
FLQEAGISGTNVTGGITAWSREIDSSVPEY
HHHHCCCCCCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLNPNLDQIQLNKEEYERFSRHLILPEVGLEGQKRLKAASILCVGTGGLGSPLLLYLAAA
CCCCCCCEEEECHHHHHHHHHEEECCCCCCCHHHHHCEEEEEEEECCCCCHHHHHHHHHC
GVGRLGIVDADVVDSSNLQRQVIHGTSWVGKPKIESAKNRIHEINPLCQVDLYNTWLTSE
CCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEEEHHEEECCC
NALEIAEPYDIVIDGTDNFPTRYLVNDACVLLDKPNVYGSIYRFEGQATVFNYEGGPNYR
CCEEECCCEEEEEECCCCCCCEEEECCEEEEEECCCCCEEEEEECCCEEEEEECCCCCCC
DLYPEPPPPGLVPSCAEGGVLGILPGIIGIIQATEAIKIILGQGTTLSGRLLLFNSLDMK
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCCCCEEEEEECCCCE
FRELKLRPNPERPVIEKLVDYQEFCGIPQAQAAEAEQKANMEEMTVQDLKQLLDSGADDF
EEEEECCCCCCCHHHHHHHCHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCE
VLLDVRNPHEYEIARIPGSVLIPLSEIESGEAIPKVKELLNGHRLIAHCKMGGRSAKALG
EEEEECCCCCEEEEECCCCEEEEHHHCCCCCCCHHHHHHHCCCEEEEEECCCCCHHHHHH
FLQEAGISGTNVTGGITAWSREIDSSVPEY
HHHHCCCCCCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]