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The map label for this gene is pyrE [H]

Identifier: 158333608

GI number: 158333608

Start: 384248

End: 384769

Strand: Direct

Name: pyrE [H]

Synonym: AM1_0409

Alternate gene names: 158333608

Gene position: 384248-384769 (Clockwise)

Preceding gene: 158333599

Following gene: 158333609

Centisome position: 5.91

GC content: 45.21

Gene sequence:

>522_bases
TTGATGAACAAAGCAGATCTGGCAAAAGCAATTTATGAAACATCCCATATCACAGGTGAGTTTTTACTGCGATCGGGACA
AATGAGCCATGAGTATTTTGATAAATATTTATTTGAAGCAAATCCAACTTTGTTGAAGGCCATCGCCGAACATTTAGCCG
CCATGATCCCCACTCCTGTGGATGCCTTAGCCGGCTTGGAAATGGGCGGCATTCCGATTGTGGCAATGCTATCTCAAATC
AGTGGATATCCATCCCTTTTTGTCAGAAAGAAGGCGAAACCGTACGGTACCTGCAAACTAGCGGAGGGAGGAGAAATTGA
AGGAAAACGGCTAGTCATCATTGAAGATGTGGTGACTTCAGGTGGACAGATCAAACTTTCAGCCCAGGACTTACGTTCCC
TAGGCGCTACGGTTACGGACGTCATGTGTGTCATTGATCGTGAAGCCGGTGGACAAGCCAATCTAAATGCTGAAGGTTTA
ACGCTCCATCCCCTATTTACGATGACAGAATTAAAGCAGTAA

Upstream 100 bases:

>100_bases
GAGGTAGGTCTGACATAATCAGGTGTCTTACCTATATAAGCAGTTCCAAGAGGGAGCCTAGCACCTAAACTAATTAAAGT
TAGCTCAATTTCCACGAGTT

Downstream 100 bases:

>100_bases
GCTCAATAGTCCCTTAGCCTCCAATGGCTTACCTTCAAATTTGCTCCTAGAACAAGAACCAGATGCAACCGTTGCTTTCA
ATTATGGATTGAAAAATAGT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MMNKADLAKAIYETSHITGEFLLRSGQMSHEYFDKYLFEANPTLLKAIAEHLAAMIPTPVDALAGLEMGGIPIVAMLSQI
SGYPSLFVRKKAKPYGTCKLAEGGEIEGKRLVIIEDVVTSGGQIKLSAQDLRSLGATVTDVMCVIDREAGGQANLNAEGL
TLHPLFTMTELKQ

Sequences:

>Translated_173_residues
MMNKADLAKAIYETSHITGEFLLRSGQMSHEYFDKYLFEANPTLLKAIAEHLAAMIPTPVDALAGLEMGGIPIVAMLSQI
SGYPSLFVRKKAKPYGTCKLAEGGEIEGKRLVIIEDVVTSGGQIKLSAQDLRSLGATVTDVMCVIDREAGGQANLNAEGL
TLHPLFTMTELKQ
>Mature_173_residues
MMNKADLAKAIYETSHITGEFLLRSGQMSHEYFDKYLFEANPTLLKAIAEHLAAMIPTPVDALAGLEMGGIPIVAMLSQI
SGYPSLFVRKKAKPYGTCKLAEGGEIEGKRLVIIEDVVTSGGQIKLSAQDLRSLGATVTDVMCVIDREAGGQANLNAEGL
TLHPLFTMTELKQ

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:
GO:0016787: Dihydroorotase, multifunctional complex type
GO:0016810: Dihydroorotase, multifunctional complex type
GO:0016812: Dihydroorotase, multifunctional complex type
GO:0046872: Dihydroorotase, multifunctional complex type

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=156, Percent_Identity=34.6153846153846, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17508631, Length=174, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17554964, Length=156, Percent_Identity=41.025641025641, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17933654, Length=156, Percent_Identity=36.5384615384615, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 18669; Mature: 18669

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNKADLAKAIYETSHITGEFLLRSGQMSHEYFDKYLFEANPTLLKAIAEHLAAMIPTPV
CCCHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCH
DALAGLEMGGIPIVAMLSQISGYPSLFVRKKAKPYGTCKLAEGGEIEGKRLVIIEDVVTS
HHHHCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCCCCCEEEEEEHHHCC
GGQIKLSAQDLRSLGATVTDVMCVIDREAGGQANLNAEGLTLHPLFTMTELKQ
CCEEEEEHHHHHHHCCHHHHHHHEEECCCCCCCCCCCCCEEECHHHHHHHHCC
>Mature Secondary Structure
MMNKADLAKAIYETSHITGEFLLRSGQMSHEYFDKYLFEANPTLLKAIAEHLAAMIPTPV
CCCHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCH
DALAGLEMGGIPIVAMLSQISGYPSLFVRKKAKPYGTCKLAEGGEIEGKRLVIIEDVVTS
HHHHCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCCCCCEEEEEEHHHCC
GGQIKLSAQDLRSLGATVTDVMCVIDREAGGQANLNAEGLTLHPLFTMTELKQ
CCEEEEEHHHHHHHCCHHHHHHHEEECCCCCCCCCCCCCEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA