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The map label for this gene is yhdJ [H]

Identifier: 158333607

GI number: 158333607

Start: 383207

End: 384076

Strand: Reverse

Name: yhdJ [H]

Synonym: AM1_0408

Alternate gene names: 158333607

Gene position: 384076-383207 (Counterclockwise)

Preceding gene: 158333614

Following gene: 158333606

Centisome position: 5.91

GC content: 42.87

Gene sequence:

>870_bases
TTGAAACTCGTCAAAAATATTACAAAAACATCACCGAAGGTCGATATTACGATTCAAGGAGATTGCCTCGATATAGCTCC
CAAACTGCTTCAAGGCAGTATCAACTTGTTAATTCTTGACCCACCTTATAACTTGAATAAAGTCTTTAATGGTCGAACGT
TTGCCAAGCAATCTGTTGCAGCCTATACCGAATGGTTAGATTATATTTGTTCTAAGCTCAAACCCCTACTACGTCCTGAT
GCCTCGATATATATCTGTGGTGACTGGTTCTCATCGGCTTCAATTTTCACAGTTGCAACTTCTCATTTTCAGGTCCGGAA
CCGGATCACATGGGAGCGCGAGAAAGGGAGAGGCGCCCATTCAAATTGGAAAAATGCGAGTGAAGATATTTGGTTTTGCA
CAAACTCGAACTCATACACCTTCAATAGTCAGGCTGTGAAGCAACGACGACGGGTACTAGCACCCTATCGTCGTCCAGAT
GGGTCTCCTAAAGATTGGTCTCAAACGGAAAATGGCAACTTTAGAGATACCTTTGCGTCTAATTTATGGACTGATATTTC
GATACCTTTTTGGTCCATGCCAGAAAATACTGATCACCCCACTCAAAAAAGCGAGAAATTAGTTGCAAAGCTCATACTGG
CAAGTAGCAACCCTGGAGATACTGTTCTAGATCCTTTCTTGGGCAGTGGCACAACGTCTGTTGTGGCTAAAAAACTGCAA
AGACAATTTATCGGCATAGAAATTGATGAGGAGTATTGCTTGTTGTCCGAACGGCGTATCGAACTAGCTGACTCCAACCC
AGCTATTCAAGGATTTGCCCAAGAAATCTTCTGGGAACGGAATACAATAATTCCCCAAGGGAACCAGTGA

Upstream 100 bases:

>100_bases
AAGACACCTGATTATGTCAGACCTACCTCAAACGCAAAAGAAAAGAGCTCCTATGAACCGTACATTGACCCTCAGTACGG
CAGACCGTGAGCGATTGAGA

Downstream 100 bases:

>100_bases
TTTTTAACCGAAGTGGGGAAAACCATGTATGTGCCAATTATTGGACATCCAGCTCTACTTCTCAAATCTTCACCGAAGAA
TGATTTAATGCAAAAGTAAT

Product: DNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKLVKNITKTSPKVDITIQGDCLDIAPKLLQGSINLLILDPPYNLNKVFNGRTFAKQSVAAYTEWLDYICSKLKPLLRPD
ASIYICGDWFSSASIFTVATSHFQVRNRITWEREKGRGAHSNWKNASEDIWFCTNSNSYTFNSQAVKQRRRVLAPYRRPD
GSPKDWSQTENGNFRDTFASNLWTDISIPFWSMPENTDHPTQKSEKLVAKLILASSNPGDTVLDPFLGSGTTSVVAKKLQ
RQFIGIEIDEEYCLLSERRIELADSNPAIQGFAQEIFWERNTIIPQGNQ

Sequences:

>Translated_289_residues
MKLVKNITKTSPKVDITIQGDCLDIAPKLLQGSINLLILDPPYNLNKVFNGRTFAKQSVAAYTEWLDYICSKLKPLLRPD
ASIYICGDWFSSASIFTVATSHFQVRNRITWEREKGRGAHSNWKNASEDIWFCTNSNSYTFNSQAVKQRRRVLAPYRRPD
GSPKDWSQTENGNFRDTFASNLWTDISIPFWSMPENTDHPTQKSEKLVAKLILASSNPGDTVLDPFLGSGTTSVVAKKLQ
RQFIGIEIDEEYCLLSERRIELADSNPAIQGFAQEIFWERNTIIPQGNQ
>Mature_289_residues
MKLVKNITKTSPKVDITIQGDCLDIAPKLLQGSINLLILDPPYNLNKVFNGRTFAKQSVAAYTEWLDYICSKLKPLLRPD
ASIYICGDWFSSASIFTVATSHFQVRNRITWEREKGRGAHSNWKNASEDIWFCTNSNSYTFNSQAVKQRRRVLAPYRRPD
GSPKDWSQTENGNFRDTFASNLWTDISIPFWSMPENTDHPTQKSEKLVAKLILASSNPGDTVLDPFLGSGTTSVVAKKLQ
RQFIGIEIDEEYCLLSERRIELADSNPAIQGFAQEIFWERNTIIPQGNQ

Specific function: Methylates the second adenine of the NsiI recognition sequence (5'-ATGCAT-3') [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=264, Percent_Identity=32.9545454545455, Blast_Score=127, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32830; Mature: 32830

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVKNITKTSPKVDITIQGDCLDIAPKLLQGSINLLILDPPYNLNKVFNGRTFAKQSVA
CCHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCHHHCCCCHHHHHHHH
AYTEWLDYICSKLKPLLRPDASIYICGDWFSSASIFTVATSHFQVRNRITWEREKGRGAH
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEHHHHHHHHCCCEECCCCCCCC
SNWKNASEDIWFCTNSNSYTFNSQAVKQRRRVLAPYRRPDGSPKDWSQTENGNFRDTFAS
CCCCCCCCCEEEEECCCCEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
NLWTDISIPFWSMPENTDHPTQKSEKLVAKLILASSNPGDTVLDPFLGSGTTSVVAKKLQ
HCEEECCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
RQFIGIEIDEEYCLLSERRIELADSNPAIQGFAQEIFWERNTIIPQGNQ
HHHCCEEECCHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKLVKNITKTSPKVDITIQGDCLDIAPKLLQGSINLLILDPPYNLNKVFNGRTFAKQSVA
CCHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCHHHCCCCHHHHHHHH
AYTEWLDYICSKLKPLLRPDASIYICGDWFSSASIFTVATSHFQVRNRITWEREKGRGAH
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEHHHHHHHHCCCEECCCCCCCC
SNWKNASEDIWFCTNSNSYTFNSQAVKQRRRVLAPYRRPDGSPKDWSQTENGNFRDTFAS
CCCCCCCCCEEEEECCCCEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
NLWTDISIPFWSMPENTDHPTQKSEKLVAKLILASSNPGDTVLDPFLGSGTTSVVAKKLQ
HCEEECCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
RQFIGIEIDEEYCLLSERRIELADSNPAIQGFAQEIFWERNTIIPQGNQ
HHHCCEEECCHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 1459953; 2835774 [H]