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The map label for this gene is wcaI [H]

Identifier: 158333586

GI number: 158333586

Start: 357699

End: 358985

Strand: Reverse

Name: wcaI [H]

Synonym: AM1_0387

Alternate gene names: 158333586

Gene position: 358985-357699 (Counterclockwise)

Preceding gene: 158333587

Following gene: 158333585

Centisome position: 5.52

GC content: 39.32

Gene sequence:

>1287_bases
TTGATTTACAGTCTGAACTATTTCCCTGAGTTGATTGGAATTGGAAAGTATAATACCGAGATGGCGAATTGGCTGGTAAA
TGAGGGTTATGATGTTGATGTTGTATGTGCCCCCCCTTATTACCCTAGTTGGGAATTAAGTCCAGGGTTTTCTGGCTGGC
GTTACTCGTGTAATCACTATGGCTTGGTCTCGGTCCATCGTTGTCCAGTTTGGTTGCCTAAAAAACTTTCCGGTATTAAT
CGCATATTGCACTTGTTTTCTTTTGCAATTTCAAGTGCAATACAGTTTATTTGGCTGATTTTCAAAAAAAAGATATCTGG
AGTTGAGAGCATCATCTTCTTGGTAGAACCTCCGATCTTTTGTTTACCAGCAGTTTTGATTGCATCAAAAATATTTCAGT
TTAAAGTTTGGCTACATGTACAAGACTTTGAAATTGATGCAGGTTTCTCTCTGGGTTTATTGCCAAGCTTTCCTCGATTA
ACTGTATTAGTGGGATCTATTGAAAACTGGTTAATGCAGAAAGTACAGGTTGTTTCAACCATCAGTGCTCAAATGAGCTT
GCGGCTCTATCAGAAAGGAGTAAAGCTAGATAAGTGTGTATACTTCCCAAACTGGGTAGATTGTAATGCGATCCATCCTA
TAAATGAAACAAATGAGTACCGTGCACAATTAGGTATATCAGAAGACAAGATTGTATGCCTCTATTCTGGTAACTTAGGT
GCTAAGCAAGGAATAAGTATCCTTATAGAAGCTGCTCGAATTCTTGAATGCACTGAAAAGATCGTATTTGTAATTGCTGG
ACAAGGTCCAATGAAAAGGGTGCTGGTTGAGCAAGCCCGTTGTTTGTCAAACATTTATTTCTTAGATTTACAGCCATCAG
AACGTTTCAATGCGTTGTTAAATATGGCTGATATTCATTTGCTTCCTCAAGCATCAGGTGTTGGAGACTTGGTTTTACCT
TCAAAGCTAAAAGCGATGTTAGCCTCTGGAAGGCCTGTTATTGCAACGGCCTATCCTGGTACTCAAGTATTTAATATTGT
ACAAACCTGTGGTGTTGCCGTTCCTCCAGAAGAGGTCCAAGAGTTGACTAAGGCAATAGCTGATTTGGCAAATCATCATC
AAAAAAGACAAAAGTTAGGTAGTGAGGGTCGAAAATATGCTTTATCCCATTGGGACCGAGAGCAGGTATTGAGTCAGTTT
GAGAGCAAATTGCGAAAACTTATTTTCACTGGAAAGCTAGTAAATCAAGATTTAGACTCTGAGGCTCAAAAGACAATTAC
CGCTTAG

Upstream 100 bases:

>100_bases
TAAATTAAATGATCATGTCAGAGAATCTTTTCTGATTCTCTTTTTTTTGTGATTATTTCCCCTTCAATTGTCTTAATTAT
TAATCATGAAGCCACATATT

Downstream 100 bases:

>100_bases
TGTTTATTTGTGAATTCACATCAAATCCAACCATTTACTGGTGGTAAAAATAACTCTGACATAAAGATTTTTTGGTAACG
CCTATTTTTTAGTGATAATA

Product: glycosyl transferase, group 1 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MIYSLNYFPELIGIGKYNTEMANWLVNEGYDVDVVCAPPYYPSWELSPGFSGWRYSCNHYGLVSVHRCPVWLPKKLSGIN
RILHLFSFAISSAIQFIWLIFKKKISGVESIIFLVEPPIFCLPAVLIASKIFQFKVWLHVQDFEIDAGFSLGLLPSFPRL
TVLVGSIENWLMQKVQVVSTISAQMSLRLYQKGVKLDKCVYFPNWVDCNAIHPINETNEYRAQLGISEDKIVCLYSGNLG
AKQGISILIEAARILECTEKIVFVIAGQGPMKRVLVEQARCLSNIYFLDLQPSERFNALLNMADIHLLPQASGVGDLVLP
SKLKAMLASGRPVIATAYPGTQVFNIVQTCGVAVPPEEVQELTKAIADLANHHQKRQKLGSEGRKYALSHWDREQVLSQF
ESKLRKLIFTGKLVNQDLDSEAQKTITA

Sequences:

>Translated_428_residues
MIYSLNYFPELIGIGKYNTEMANWLVNEGYDVDVVCAPPYYPSWELSPGFSGWRYSCNHYGLVSVHRCPVWLPKKLSGIN
RILHLFSFAISSAIQFIWLIFKKKISGVESIIFLVEPPIFCLPAVLIASKIFQFKVWLHVQDFEIDAGFSLGLLPSFPRL
TVLVGSIENWLMQKVQVVSTISAQMSLRLYQKGVKLDKCVYFPNWVDCNAIHPINETNEYRAQLGISEDKIVCLYSGNLG
AKQGISILIEAARILECTEKIVFVIAGQGPMKRVLVEQARCLSNIYFLDLQPSERFNALLNMADIHLLPQASGVGDLVLP
SKLKAMLASGRPVIATAYPGTQVFNIVQTCGVAVPPEEVQELTKAIADLANHHQKRQKLGSEGRKYALSHWDREQVLSQF
ESKLRKLIFTGKLVNQDLDSEAQKTITA
>Mature_428_residues
MIYSLNYFPELIGIGKYNTEMANWLVNEGYDVDVVCAPPYYPSWELSPGFSGWRYSCNHYGLVSVHRCPVWLPKKLSGIN
RILHLFSFAISSAIQFIWLIFKKKISGVESIIFLVEPPIFCLPAVLIASKIFQFKVWLHVQDFEIDAGFSLGLLPSFPRL
TVLVGSIENWLMQKVQVVSTISAQMSLRLYQKGVKLDKCVYFPNWVDCNAIHPINETNEYRAQLGISEDKIVCLYSGNLG
AKQGISILIEAARILECTEKIVFVIAGQGPMKRVLVEQARCLSNIYFLDLQPSERFNALLNMADIHLLPQASGVGDLVLP
SKLKAMLASGRPVIATAYPGTQVFNIVQTCGVAVPPEEVQELTKAIADLANHHQKRQKLGSEGRKYALSHWDREQVLSQF
ESKLRKLIFTGKLVNQDLDSEAQKTITA

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788363, Length=417, Percent_Identity=37.8896882494005, Blast_Score=250, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 48055; Mature: 48055

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYSLNYFPELIGIGKYNTEMANWLVNEGYDVDVVCAPPYYPSWELSPGFSGWRYSCNHY
CEEECCCCHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GLVSVHRCPVWLPKKLSGINRILHLFSFAISSAIQFIWLIFKKKISGVESIIFLVEPPIF
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCHH
CLPAVLIASKIFQFKVWLHVQDFEIDAGFSLGLLPSFPRLTVLVGSIENWLMQKVQVVST
HHHHHHHHHHHHEEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ISAQMSLRLYQKGVKLDKCVYFPNWVDCNAIHPINETNEYRAQLGISEDKIVCLYSGNLG
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCC
AKQGISILIEAARILECTEKIVFVIAGQGPMKRVLVEQARCLSNIYFLDLQPSERFNALL
CHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHH
NMADIHLLPQASGVGDLVLPSKLKAMLASGRPVIATAYPGTQVFNIVQTCGVAVPPEEVQ
HHHHEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHH
ELTKAIADLANHHQKRQKLGSEGRKYALSHWDREQVLSQFESKLRKLIFTGKLVNQDLDS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EAQKTITA
HHHHHCCC
>Mature Secondary Structure
MIYSLNYFPELIGIGKYNTEMANWLVNEGYDVDVVCAPPYYPSWELSPGFSGWRYSCNHY
CEEECCCCHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GLVSVHRCPVWLPKKLSGINRILHLFSFAISSAIQFIWLIFKKKISGVESIIFLVEPPIF
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCHH
CLPAVLIASKIFQFKVWLHVQDFEIDAGFSLGLLPSFPRLTVLVGSIENWLMQKVQVVST
HHHHHHHHHHHHEEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ISAQMSLRLYQKGVKLDKCVYFPNWVDCNAIHPINETNEYRAQLGISEDKIVCLYSGNLG
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCC
AKQGISILIEAARILECTEKIVFVIAGQGPMKRVLVEQARCLSNIYFLDLQPSERFNALL
CHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHH
NMADIHLLPQASGVGDLVLPSKLKAMLASGRPVIATAYPGTQVFNIVQTCGVAVPPEEVQ
HHHHEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHH
ELTKAIADLANHHQKRQKLGSEGRKYALSHWDREQVLSQFESKLRKLIFTGKLVNQDLDS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EAQKTITA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503 [H]