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The map label for this gene is thiF [H]

Identifier: 158333534

GI number: 158333534

Start: 304808

End: 305443

Strand: Direct

Name: thiF [H]

Synonym: AM1_0333

Alternate gene names: 158333534

Gene position: 304808-305443 (Clockwise)

Preceding gene: 158333533

Following gene: 158333535

Centisome position: 4.69

GC content: 50.0

Gene sequence:

>636_bases
ATGACTGAACAATTTTTTCATGAGCAGCTATATCGATCTACCCAACAGATGGCGATGCTCCGAGATTATCCGGTGACCGT
ATGTGGCGCAGGTGCACTAGGGGCCAACTTAGTGGAAACCCTGGCCCGCTCCGGCTACGGAAAACTTAAAGTGATTGACT
GCGATCGCATCGAGACTCGCAACCTTTCTACCCAGCCCTACTATCGATCTGATGTCGGGGCCTATAAAGGCAAGATCCTA
GCCAATATGCTGTATCGAGCTCTGGGAACTACGGTGGACGTGATTCCCAAGCAGCTCACCGCCGCCAATACCCAGTCACT
ATTACGGGGGAGTTCTTTGGTTATTGATACCTTCGATAACAGTGTTTCTCGCCAAGCGGTTAAAGACTATTGTTTAGAGC
AGGATATTCCTTGCCTCCATGCTGGATTGGCTGCGGAGTATGCAGAAGTGGTTTGGGAGCCGGAGTATCAAGTGCCATCG
GCAACCCATGATGATATCTGCGACTATCCCCTAGCGCGGAATTTGGTGTTGTTGACCGTTGCGATCGCAAGTGAAACCAT
CACTCGATTCATCACGGAAGGTCGTCAACAAAGCTGTACGATTACCCTTCGAGACTTTGTGATTCACACCAGTTGA

Upstream 100 bases:

>100_bases
CTCACCGTATTGAGTTCAGGTATTTGTCTCAGCGGACGCGATCTCGATTTTGATCTGGAATCTCTTGTCGGGGTGATTGA
ACAGGAGGAAGAAGATTGGT

Downstream 100 bases:

>100_bases
TAGTTGCAAAATCTCTGTCAGGGATTGTCATGACACAATTAGACTGAAAATCTTCTAGAAATTCTGAGTGCAATAGTGTA
ATCTCTTTTTCTGAACGCTT

Product: ThiF domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTEQFFHEQLYRSTQQMAMLRDYPVTVCGAGALGANLVETLARSGYGKLKVIDCDRIETRNLSTQPYYRSDVGAYKGKIL
ANMLYRALGTTVDVIPKQLTAANTQSLLRGSSLVIDTFDNSVSRQAVKDYCLEQDIPCLHAGLAAEYAEVVWEPEYQVPS
ATHDDICDYPLARNLVLLTVAIASETITRFITEGRQQSCTITLRDFVIHTS

Sequences:

>Translated_211_residues
MTEQFFHEQLYRSTQQMAMLRDYPVTVCGAGALGANLVETLARSGYGKLKVIDCDRIETRNLSTQPYYRSDVGAYKGKIL
ANMLYRALGTTVDVIPKQLTAANTQSLLRGSSLVIDTFDNSVSRQAVKDYCLEQDIPCLHAGLAAEYAEVVWEPEYQVPS
ATHDDICDYPLARNLVLLTVAIASETITRFITEGRQQSCTITLRDFVIHTS
>Mature_210_residues
TEQFFHEQLYRSTQQMAMLRDYPVTVCGAGALGANLVETLARSGYGKLKVIDCDRIETRNLSTQPYYRSDVGAYKGKILA
NMLYRALGTTVDVIPKQLTAANTQSLLRGSSLVIDTFDNSVSRQAVKDYCLEQDIPCLHAGLAAEYAEVVWEPEYQVPSA
THDDICDYPLARNLVLLTVAIASETITRFITEGRQQSCTITLRDFVIHTS

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 23510; Mature: 23379

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQFFHEQLYRSTQQMAMLRDYPVTVCGAGALGANLVETLARSGYGKLKVIDCDRIETR
CCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCC
NLSTQPYYRSDVGAYKGKILANMLYRALGTTVDVIPKQLTAANTQSLLRGSSLVIDTFDN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
SVSRQAVKDYCLEQDIPCLHAGLAAEYAEVVWEPEYQVPSATHDDICDYPLARNLVLLTV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHH
AIASETITRFITEGRQQSCTITLRDFVIHTS
HHHHHHHHHHHHCCCCCCEEEEEEHEEEECC
>Mature Secondary Structure 
TEQFFHEQLYRSTQQMAMLRDYPVTVCGAGALGANLVETLARSGYGKLKVIDCDRIETR
CHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCC
NLSTQPYYRSDVGAYKGKILANMLYRALGTTVDVIPKQLTAANTQSLLRGSSLVIDTFDN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
SVSRQAVKDYCLEQDIPCLHAGLAAEYAEVVWEPEYQVPSATHDDICDYPLARNLVLLTV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHH
AIASETITRFITEGRQQSCTITLRDFVIHTS
HHHHHHHHHHHHCCCCCCEEEEEEHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]