Click here to switch to the map view.

The map label for this gene is nfi [H]

Identifier: 158333530

GI number: 158333530

Start: 300736

End: 301416

Strand: Direct

Name: nfi [H]

Synonym: AM1_0329

Alternate gene names: 158333530

Gene position: 300736-301416 (Clockwise)

Preceding gene: 158333529

Following gene: 158333531

Centisome position: 4.62

GC content: 49.34

Gene sequence:

>681_bases
ATGCCCCACAAACATCACTTTAACCAGATGAAAAATCCTGAATCGGCCTTTGCCCTTCAGGAAAAACTGCGGCACCAAGT
CATCCGAGAGGACGATTTTGGTCCCCTTGAAACGGTGGCAGGAGTGGATGTTGGATTTGAGGATAAGGATCAAATTGCCT
GTGCTGTAGTTGCAGTACTGAGACTATCTGATCTCAAGGTGCAGGAATCTGTGGCGATTCGTCTTCCGGTTACCTTCCCC
TATGTACCGGGATTGCTAGCATTCCGAGAGGTTCCTATCGTCATGGAAGCCTTGGCAAGTCTGAAAACTCGGCCTGGACT
CCTAATCTGTGATGGCAATGGCACCATCCATCCTCGTCGGTGTGGATTTGCTTGCCATTTAGGAATTGTTAGTGGACTGC
CGTCTATCGGAGTAGCCAAAACTCCTTTCATTGGGGAATTTCAACCTCTAGCCGATGATCGTGGGGCATGGCAGCCGATC
ACAGATGGGGGAGAGGTTATTGGGGCAGCCCTGCGGACACAAACAAAGGTTAAACCTATCTTTGTGTCCATTGGCCATCG
CATTAGTTTGCAAAGTGCTATTGATTTGGTGCTCCAATGCGCTCCCGCATATCGTTTACCTGAAACCACTCGCCAAGCCG
ATCAGTTATCCAAGGGCAATGCCCAACCTGTCATCTTATAA

Upstream 100 bases:

>100_bases
GTAACAAGAAGGAGATCCGATTTATTTTAAGTCAGATTAAAGCAGTTAAACCTACCTCCTGAGAGGGGTTGAAAGTCCTA
GCTCTCTGTAGGAGATTGTT

Downstream 100 bases:

>100_bases
ACAATCCGCACCCCAACAAGAAAAGCTTACATACTTGGTTGAGTCCTTACTTTATAGGGAGGGCTGCAGGTTCAAATCCT
GCTTTGCTTCGCGCAAAAAT

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MPHKHHFNQMKNPESAFALQEKLRHQVIREDDFGPLETVAGVDVGFEDKDQIACAVVAVLRLSDLKVQESVAIRLPVTFP
YVPGLLAFREVPIVMEALASLKTRPGLLICDGNGTIHPRRCGFACHLGIVSGLPSIGVAKTPFIGEFQPLADDRGAWQPI
TDGGEVIGAALRTQTKVKPIFVSIGHRISLQSAIDLVLQCAPAYRLPETTRQADQLSKGNAQPVIL

Sequences:

>Translated_226_residues
MPHKHHFNQMKNPESAFALQEKLRHQVIREDDFGPLETVAGVDVGFEDKDQIACAVVAVLRLSDLKVQESVAIRLPVTFP
YVPGLLAFREVPIVMEALASLKTRPGLLICDGNGTIHPRRCGFACHLGIVSGLPSIGVAKTPFIGEFQPLADDRGAWQPI
TDGGEVIGAALRTQTKVKPIFVSIGHRISLQSAIDLVLQCAPAYRLPETTRQADQLSKGNAQPVIL
>Mature_225_residues
PHKHHFNQMKNPESAFALQEKLRHQVIREDDFGPLETVAGVDVGFEDKDQIACAVVAVLRLSDLKVQESVAIRLPVTFPY
VPGLLAFREVPIVMEALASLKTRPGLLICDGNGTIHPRRCGFACHLGIVSGLPSIGVAKTPFIGEFQPLADDRGAWQPIT
DGGEVIGAALRTQTKVKPIFVSIGHRISLQSAIDLVLQCAPAYRLPETTRQADQLSKGNAQPVIL

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=236, Percent_Identity=37.7118644067797, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI257467548, Length=231, Percent_Identity=31.1688311688312, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI257467550, Length=231, Percent_Identity=31.1688311688312, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI87082357, Length=202, Percent_Identity=41.0891089108911, Blast_Score=170, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI71981509, Length=134, Percent_Identity=33.5820895522388, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 24556; Mature: 24425

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHKHHFNQMKNPESAFALQEKLRHQVIREDDFGPLETVAGVDVGFEDKDQIACAVVAVL
CCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHH
RLSDLKVQESVAIRLPVTFPYVPGLLAFREVPIVMEALASLKTRPGLLICDGNGTIHPRR
HHHCCEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEECCCC
CGFACHLGIVSGLPSIGVAKTPFIGEFQPLADDRGAWQPITDGGEVIGAALRTQTKVKPI
CCCEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
FVSIGHRISLQSAIDLVLQCAPAYRLPETTRQADQLSKGNAQPVIL
EEECCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PHKHHFNQMKNPESAFALQEKLRHQVIREDDFGPLETVAGVDVGFEDKDQIACAVVAVL
CCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHH
RLSDLKVQESVAIRLPVTFPYVPGLLAFREVPIVMEALASLKTRPGLLICDGNGTIHPRR
HHHCCEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEECCCC
CGFACHLGIVSGLPSIGVAKTPFIGEFQPLADDRGAWQPITDGGEVIGAALRTQTKVKPI
CCCEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
FVSIGHRISLQSAIDLVLQCAPAYRLPETTRQADQLSKGNAQPVIL
EEECCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA