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The map label for this gene is pucA [H]

Identifier: 158333477

GI number: 158333477

Start: 244827

End: 245969

Strand: Reverse

Name: pucA [H]

Synonym: AM1_0249

Alternate gene names: 158333477

Gene position: 245969-244827 (Counterclockwise)

Preceding gene: 158333479

Following gene: 158333474

Centisome position: 3.78

GC content: 44.27

Gene sequence:

>1143_bases
ATGAAAGAGCTACAAGAAATCATTCCTTTTGTTATTCAAGCCGATCATCAAGATCCGTCAATTATTGTAGCGACTGTGTT
GCAAGCGGAGGAATCAAGCTACTGCAGGTCTGGAACTCGGATTCTATTGACCAAAGCTGATCAAACCATTCGGGCGATTT
GTGAGGATTATATTGATCAGAAAATTATTAACCAAGTTAAACAGCTTGTTCAGATTAATGGCTGTCCCCAAGTGTATCGA
TATCATGTCGATCAACTTCACACACTGGATAAGACAAGCTCTCGAGGCGGAACTACAATTTTACTGGAGCCTCTTAATAG
TCGATCCACTAGAGCTCAGTTTGATTTCATTGCTGAATGTTATGACGAGAACCAATCCGGGGCAATCGCAACTGTCTTTA
CTGTAGAAGGTGATATTCCTGCCCAAGTCGCCTCCCGATTATTTCTCAAGATGGATGGTTCAGTGGCCAATCAAATTGGG
GACCATTTTCTCACCAATCTTATTTTTCAAGATACGGTCAAAATATTGGCAGCGGGGCAAACTCGGACTAGGAGCTATCC
CTTTATTGACGGTTATGTCCAAGCCCTGATTGAAGTCATTATTCCCCCCATCCAGCTCTATATCTTTGGTATTACACCTT
ACACTTCCCCCATTACACTCCTCGCAAAACAGTTGGGGTGGACTGTTGTTATCGTTGAGCATCCATCGCGAGAGATTAAA
CTCAACCATTTCCCAGAGGCAGATCATATTCTGCAAGTCGAGCCTCAGGACTTGCATCAACAAATTCAGCTCTCATCATA
TTCAGCTGCTGTTGTGATGACTGGAGACTTTGAGCAGGATTTGGCAATCGTAAAGTTTCTTTGGTCTAGTGCCATAGGCT
ACTTAGGTGTTCCTGGCACAAAAGCAAGGACTCAACAGCTTATTCAAGGTCTACAGGATGGGGGCTCTCTTACGCCGGAG
CAACTCAATCGGCTCCATGGCCCCATTGGCCTAGATATCGGCGCGGAAACACCTAGTGAAATTGCCTTAGCGATAGTGTC
AGAGATCCAGGCTGTTGTTAAACATCGAACCGGATCATTTTTACGAGAACAGCAAAACTCGGTCTATGATTCTGCTCCTG
ATTTGATTTCCCTAATCCAATAG

Upstream 100 bases:

>100_bases
ATTGATGACTTTTAGAGCAGAAAAGCATACCTCTAACTAAATTTGGAAATAGATTTTTTCCGTTTAGAGCAATCACTTTA
CAGTTTTGCTACCCGTTCGA

Downstream 100 bases:

>100_bases
AGTCAGACCATCATCAGATTTGAGTCTCCTTCAACTCTCAACCGGGAGGTGTCGCGTCAGGGGCTGGTTCAGGGATGGGA
TTAGGTGCAGTAACAGATGG

Product: hypothetical protein

Products: NA

Alternate protein names: XDHase subunit A [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKELQEIIPFVIQADHQDPSIIVATVLQAEESSYCRSGTRILLTKADQTIRAICEDYIDQKIINQVKQLVQINGCPQVYR
YHVDQLHTLDKTSSRGGTTILLEPLNSRSTRAQFDFIAECYDENQSGAIATVFTVEGDIPAQVASRLFLKMDGSVANQIG
DHFLTNLIFQDTVKILAAGQTRTRSYPFIDGYVQALIEVIIPPIQLYIFGITPYTSPITLLAKQLGWTVVIVEHPSREIK
LNHFPEADHILQVEPQDLHQQIQLSSYSAAVVMTGDFEQDLAIVKFLWSSAIGYLGVPGTKARTQQLIQGLQDGGSLTPE
QLNRLHGPIGLDIGAETPSEIALAIVSEIQAVVKHRTGSFLREQQNSVYDSAPDLISLIQ

Sequences:

>Translated_380_residues
MKELQEIIPFVIQADHQDPSIIVATVLQAEESSYCRSGTRILLTKADQTIRAICEDYIDQKIINQVKQLVQINGCPQVYR
YHVDQLHTLDKTSSRGGTTILLEPLNSRSTRAQFDFIAECYDENQSGAIATVFTVEGDIPAQVASRLFLKMDGSVANQIG
DHFLTNLIFQDTVKILAAGQTRTRSYPFIDGYVQALIEVIIPPIQLYIFGITPYTSPITLLAKQLGWTVVIVEHPSREIK
LNHFPEADHILQVEPQDLHQQIQLSSYSAAVVMTGDFEQDLAIVKFLWSSAIGYLGVPGTKARTQQLIQGLQDGGSLTPE
QLNRLHGPIGLDIGAETPSEIALAIVSEIQAVVKHRTGSFLREQQNSVYDSAPDLISLIQ
>Mature_380_residues
MKELQEIIPFVIQADHQDPSIIVATVLQAEESSYCRSGTRILLTKADQTIRAICEDYIDQKIINQVKQLVQINGCPQVYR
YHVDQLHTLDKTSSRGGTTILLEPLNSRSTRAQFDFIAECYDENQSGAIATVFTVEGDIPAQVASRLFLKMDGSVANQIG
DHFLTNLIFQDTVKILAAGQTRTRSYPFIDGYVQALIEVIIPPIQLYIFGITPYTSPITLLAKQLGWTVVIVEHPSREIK
LNHFPEADHILQVEPQDLHQQIQLSSYSAAVVMTGDFEQDLAIVKFLWSSAIGYLGVPGTKARTQQLIQGLQDGGSLTPE
QLNRLHGPIGLDIGAETPSEIALAIVSEIQAVVKHRTGSFLREQQNSVYDSAPDLISLIQ

Specific function: Oxidizes hypoxanthine and xanthine to uric acid. PucA subunit could exert a molybdenum cofactor recruiting function [H]

COG id: COG1975

COG function: function code O; Xanthine and CO dehydrogenases maturation factor, XdhC/CoxF family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789240, Length=269, Percent_Identity=22.6765799256506, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003777 [H]

Pfam domain/function: PF02625 XdhC_CoxI [H]

EC number: =1.17.1.4 [H]

Molecular weight: Translated: 42122; Mature: 42122

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELQEIIPFVIQADHQDPSIIVATVLQAEESSYCRSGTRILLTKADQTIRAICEDYIDQ
CCHHHHHHHHHEECCCCCCCEEEHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
KIINQVKQLVQINGCPQVYRYHVDQLHTLDKTSSRGGTTILLEPLNSRSTRAQFDFIAEC
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
YDENQSGAIATVFTVEGDIPAQVASRLFLKMDGSVANQIGDHFLTNLIFQDTVKILAAGQ
HCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
TRTRSYPFIDGYVQALIEVIIPPIQLYIFGITPYTSPITLLAKQLGWTVVIVEHPSREIK
CCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEE
LNHFPEADHILQVEPQDLHQQIQLSSYSAAVVMTGDFEQDLAIVKFLWSSAIGYLGVPGT
ECCCCCCCCEEEECHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCC
KARTQQLIQGLQDGGSLTPEQLNRLHGPIGLDIGAETPSEIALAIVSEIQAVVKHRTGSF
HHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
LREQQNSVYDSAPDLISLIQ
HHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure
MKELQEIIPFVIQADHQDPSIIVATVLQAEESSYCRSGTRILLTKADQTIRAICEDYIDQ
CCHHHHHHHHHEECCCCCCCEEEHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
KIINQVKQLVQINGCPQVYRYHVDQLHTLDKTSSRGGTTILLEPLNSRSTRAQFDFIAEC
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
YDENQSGAIATVFTVEGDIPAQVASRLFLKMDGSVANQIGDHFLTNLIFQDTVKILAAGQ
HCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
TRTRSYPFIDGYVQALIEVIIPPIQLYIFGITPYTSPITLLAKQLGWTVVIVEHPSREIK
CCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEE
LNHFPEADHILQVEPQDLHQQIQLSSYSAAVVMTGDFEQDLAIVKFLWSSAIGYLGVPGT
ECCCCCCCCEEEECHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCC
KARTQQLIQGLQDGGSLTPEQLNRLHGPIGLDIGAETPSEIALAIVSEIQAVVKHRTGSF
HHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
LREQQNSVYDSAPDLISLIQ
HHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]