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The map label for this gene is merA [H]

Identifier: 158333369

GI number: 158333369

Start: 138414

End: 139961

Strand: Direct

Name: merA [H]

Synonym: AM1_0141

Alternate gene names: 158333369

Gene position: 138414-139961 (Clockwise)

Preceding gene: 158333368

Following gene: 158333370

Centisome position: 2.13

GC content: 51.29

Gene sequence:

>1548_bases
ATGACACAATCTTCGTTCTCTGGTGTGAACATCCCTCCCATGGATGTTCATAATCGCAAGCTAGTGAATAACGTTCATCC
GCCTGATTGGGTGAATCCTACTCCAGTTGAATGTTATGACCTTGTTGTGATTGGCTCAGGAACGGCGGGGTTAGTCACCG
CTAAGGGAGCAGCGGGTTTAGGGGTTGGCCTTAAGGTCGCCCTAATTGAAAAGCATCTGATGGGGGGAGATTGTTTAAAT
GTGGGCTGTGTGCCTTCTAAATGCCTGATTCGCTCCGCCCGAGTCGTTGCTGATATGCGGGATGCTCAACCCTATGGGAT
TCAGCCGCCTGCCCAGATAGATGTCGACTTTCAAGTCGTCATGGAGCGGATGCGCGAGGTTCGCGCCCGGATTAGCCCTG
TAGACTCTGCTGCTGCTGCTGCGAAGTCTGGGGTGGATATGTATTTAGGAGAGGGCAAATTCACAGGCCCCAATACCGTA
TCGGTTGATGGCAGAACTCTGCGGTTTAAAAAAGCTGTAATCACCACAGGGGCAAGGGCGGTTCGCCCTCGGATTGAGGG
AATTGAAGAAGTCGGGTATCTTACCAACGAAACGGTGTTCTCCTTAACGGAGTTACCCCAGCGCCTCGCCGTCATTGGGG
GTGGTCCCATTGGTTGTGAACTGGCCCAATCCTTTCATCGCCTTGGTAGTCAGGTGGTGCTCTTCCATCGAGGGGCTCAT
CTGATGAGTAAAGAAGATGCTGATGCGGCAGAAATTGTCCAGCAAGCCTTTTTAAAAGAAGGCATTAAGCTGGTGTTGGC
TTGTCAGATGAAGCGGGTCGAACGCACGCCTTCAGGCAAAGTGATTCACTTTGTCTGTGATGGTCGAGAAGAATCCGTGG
TGGTTGATGAAATCTTGGCGGGGGCAGGTCGTGCTCCCAATGTCGATGGCTTAAATCTGGAGGCAGTAGGGGTTGAATAT
GACAGTCGCAAGGGCGTTGCTGTCAACGACTACCTGCAAACGACAAATCCCAAAATCTATGCAGCCGGGGATATCTGTAT
GAACTGGAAGTTTACCCATGCTGCCGATGCTGCTGCCCGCATCGTCCTTAAAAACACCTTATTTTCGCCTTTTGGTATTG
GCAAATATAAACTCAGTGATTTGGTGATGCCCTGGGTGACGTTCACGGATCCTGAAATTGCCCATGTTGGTTTGTATGAG
GGAGAAGCCCAGGACCGAGGTATGGATGTCAACACCATAAAAATTCTGTTTGACGATGTGGATCGAGCCCTTGCTGATGG
GGAGGAAGAGGGCTTTGTCAAAATCCACCTTGCTAAAGGATCAGACAAAATCATCGGAGCCACCATCGTTGCCCGTCACG
CAGGCGAAATGATCAGCGAAATTACCACGGCCATGGTTGGCAAAGTGGGATTAGGCAGTATGGCGAGCGTGATTCACCCC
TATCCCACCCAGGCCGCAGCCATCAAACAGGCCGCAGATGCCTACCGTCGCACCTTACTAACGCCCAAAACCATTAAGTT
CTTGGGACTGTTGACCAAGCTGTCCTAG

Upstream 100 bases:

>100_bases
CTTAGAATTTATCCGCATAATGGTTGCGTTGATTTCGACTGATCTGGGACAGAGTTTTTCGTTTCTATCCCCATCTAATC
CTGAGGAGTTTTAGCAGCCC

Downstream 100 bases:

>100_bases
TCGCCAGCTCAAGAACTCCAAAAATATTTGGCGCGTTCTTCTGTCCAGTTACAACTGGGCAGTATGTTTGGATGTTGTTA
TCTGTAACAAGGAAGACCTG

Product: mercuric reductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MTQSSFSGVNIPPMDVHNRKLVNNVHPPDWVNPTPVECYDLVVIGSGTAGLVTAKGAAGLGVGLKVALIEKHLMGGDCLN
VGCVPSKCLIRSARVVADMRDAQPYGIQPPAQIDVDFQVVMERMREVRARISPVDSAAAAAKSGVDMYLGEGKFTGPNTV
SVDGRTLRFKKAVITTGARAVRPRIEGIEEVGYLTNETVFSLTELPQRLAVIGGGPIGCELAQSFHRLGSQVVLFHRGAH
LMSKEDADAAEIVQQAFLKEGIKLVLACQMKRVERTPSGKVIHFVCDGREESVVVDEILAGAGRAPNVDGLNLEAVGVEY
DSRKGVAVNDYLQTTNPKIYAAGDICMNWKFTHAADAAARIVLKNTLFSPFGIGKYKLSDLVMPWVTFTDPEIAHVGLYE
GEAQDRGMDVNTIKILFDDVDRALADGEEEGFVKIHLAKGSDKIIGATIVARHAGEMISEITTAMVGKVGLGSMASVIHP
YPTQAAAIKQAADAYRRTLLTPKTIKFLGLLTKLS

Sequences:

>Translated_515_residues
MTQSSFSGVNIPPMDVHNRKLVNNVHPPDWVNPTPVECYDLVVIGSGTAGLVTAKGAAGLGVGLKVALIEKHLMGGDCLN
VGCVPSKCLIRSARVVADMRDAQPYGIQPPAQIDVDFQVVMERMREVRARISPVDSAAAAAKSGVDMYLGEGKFTGPNTV
SVDGRTLRFKKAVITTGARAVRPRIEGIEEVGYLTNETVFSLTELPQRLAVIGGGPIGCELAQSFHRLGSQVVLFHRGAH
LMSKEDADAAEIVQQAFLKEGIKLVLACQMKRVERTPSGKVIHFVCDGREESVVVDEILAGAGRAPNVDGLNLEAVGVEY
DSRKGVAVNDYLQTTNPKIYAAGDICMNWKFTHAADAAARIVLKNTLFSPFGIGKYKLSDLVMPWVTFTDPEIAHVGLYE
GEAQDRGMDVNTIKILFDDVDRALADGEEEGFVKIHLAKGSDKIIGATIVARHAGEMISEITTAMVGKVGLGSMASVIHP
YPTQAAAIKQAADAYRRTLLTPKTIKFLGLLTKLS
>Mature_514_residues
TQSSFSGVNIPPMDVHNRKLVNNVHPPDWVNPTPVECYDLVVIGSGTAGLVTAKGAAGLGVGLKVALIEKHLMGGDCLNV
GCVPSKCLIRSARVVADMRDAQPYGIQPPAQIDVDFQVVMERMREVRARISPVDSAAAAAKSGVDMYLGEGKFTGPNTVS
VDGRTLRFKKAVITTGARAVRPRIEGIEEVGYLTNETVFSLTELPQRLAVIGGGPIGCELAQSFHRLGSQVVLFHRGAHL
MSKEDADAAEIVQQAFLKEGIKLVLACQMKRVERTPSGKVIHFVCDGREESVVVDEILAGAGRAPNVDGLNLEAVGVEYD
SRKGVAVNDYLQTTNPKIYAAGDICMNWKFTHAADAAARIVLKNTLFSPFGIGKYKLSDLVMPWVTFTDPEIAHVGLYEG
EAQDRGMDVNTIKILFDDVDRALADGEEEGFVKIHLAKGSDKIIGATIVARHAGEMISEITTAMVGKVGLGSMASVIHPY
PTQAAAIKQAADAYRRTLLTPKTIKFLGLLTKLS

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=466, Percent_Identity=31.3304721030043, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI50301238, Length=441, Percent_Identity=29.0249433106576, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI22035672, Length=390, Percent_Identity=28.974358974359, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI291045266, Length=420, Percent_Identity=25.7142857142857, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI33519430, Length=433, Percent_Identity=23.3256351039261, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI33519428, Length=433, Percent_Identity=23.3256351039261, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI33519426, Length=433, Percent_Identity=23.3256351039261, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI148277065, Length=433, Percent_Identity=23.3256351039261, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI148277071, Length=433, Percent_Identity=23.3256351039261, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI291045268, Length=289, Percent_Identity=26.2975778546713, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1786307, Length=478, Percent_Identity=30.3347280334728, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87081717, Length=459, Percent_Identity=26.3616557734205, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789915, Length=413, Percent_Identity=29.0556900726392, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI87082354, Length=464, Percent_Identity=24.7844827586207, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1789065, Length=254, Percent_Identity=28.740157480315, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI32565766, Length=463, Percent_Identity=30.8855291576674, Blast_Score=188, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17557007, Length=471, Percent_Identity=26.7515923566879, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71983429, Length=430, Percent_Identity=27.906976744186, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=430, Percent_Identity=27.906976744186, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=480, Percent_Identity=25.4166666666667, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=30.4166666666667, Blast_Score=176, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6325166, Length=469, Percent_Identity=27.5053304904051, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6325240, Length=479, Percent_Identity=24.0083507306889, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=31.5010570824524, Blast_Score=185, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24640551, Length=484, Percent_Identity=26.2396694214876, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24640549, Length=484, Percent_Identity=26.2396694214876, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24640553, Length=484, Percent_Identity=26.2396694214876, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI17737741, Length=472, Percent_Identity=26.0593220338983, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 55301; Mature: 55169

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSSFSGVNIPPMDVHNRKLVNNVHPPDWVNPTPVECYDLVVIGSGTAGLVTAKGAAGL
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCC
GVGLKVALIEKHLMGGDCLNVGCVPSKCLIRSARVVADMRDAQPYGIQPPAQIDVDFQVV
CCCEEEEEHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHH
MERMREVRARISPVDSAAAAAKSGVDMYLGEGKFTGPNTVSVDGRTLRFKKAVITTGARA
HHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCCCCCEEEECCCEEEEEHHHHHCCCHH
VRPRIEGIEEVGYLTNETVFSLTELPQRLAVIGGGPIGCELAQSFHRLGSQVVLFHRGAH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCEEEEEECCCH
LMSKEDADAAEIVQQAFLKEGIKLVLACQMKRVERTPSGKVIHFVCDGREESVVVDEILA
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHH
GAGRAPNVDGLNLEAVGVEYDSRKGVAVNDYLQTTNPKIYAAGDICMNWKFTHAADAAAR
CCCCCCCCCCCEEEEEEEEECCCCCCEEHHHHHCCCCEEEEECCEEEEEEECCHHHHHHH
IVLKNTLFSPFGIGKYKLSDLVMPWVTFTDPEIAHVGLYEGEAQDRGMDVNTIKILFDDV
HHHHHHCCCCCCCCCEEHHHHHCCCEEECCCCEEEEEEECCCCHHCCCCCEEEEEEHHHH
DRALADGEEEGFVKIHLAKGSDKIIGATIVARHAGEMISEITTAMVGKVGLGSMASVIHP
HHHHHCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCC
YPTQAAAIKQAADAYRRTLLTPKTIKFLGLLTKLS
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TQSSFSGVNIPPMDVHNRKLVNNVHPPDWVNPTPVECYDLVVIGSGTAGLVTAKGAAGL
CCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCC
GVGLKVALIEKHLMGGDCLNVGCVPSKCLIRSARVVADMRDAQPYGIQPPAQIDVDFQVV
CCCEEEEEHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHH
MERMREVRARISPVDSAAAAAKSGVDMYLGEGKFTGPNTVSVDGRTLRFKKAVITTGARA
HHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCCCCCEEEECCCEEEEEHHHHHCCCHH
VRPRIEGIEEVGYLTNETVFSLTELPQRLAVIGGGPIGCELAQSFHRLGSQVVLFHRGAH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCEEEEEECCCH
LMSKEDADAAEIVQQAFLKEGIKLVLACQMKRVERTPSGKVIHFVCDGREESVVVDEILA
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHH
GAGRAPNVDGLNLEAVGVEYDSRKGVAVNDYLQTTNPKIYAAGDICMNWKFTHAADAAAR
CCCCCCCCCCCEEEEEEEEECCCCCCEEHHHHHCCCCEEEEECCEEEEEEECCHHHHHHH
IVLKNTLFSPFGIGKYKLSDLVMPWVTFTDPEIAHVGLYEGEAQDRGMDVNTIKILFDDV
HHHHHHCCCCCCCCCEEHHHHHCCCEEECCCCEEEEEEECCCCHHCCCCCEEEEEEHHHH
DRALADGEEEGFVKIHLAKGSDKIIGATIVARHAGEMISEITTAMVGKVGLGSMASVIHP
HHHHHCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCC
YPTQAAAIKQAADAYRRTLLTPKTIKFLGLLTKLS
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3037534 [H]