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The map label for this gene is rsmF [H]

Identifier: 158333255

GI number: 158333255

Start: 27421

End: 28359

Strand: Reverse

Name: rsmF [H]

Synonym: AM1_0024

Alternate gene names: 158333255

Gene position: 28359-27421 (Counterclockwise)

Preceding gene: 158333258

Following gene: 158333254

Centisome position: 0.44

GC content: 50.59

Gene sequence:

>939_bases
TTGTTGACAAAATTTAGTCTGAAGTTATGGCCTGACCCTCAGCAACAACAAGCATTTATACAGGCATTGACGGAGCCACA
GCCCTATAGCCCTTGTATTGCTTGGATCAAAGGGCGATCAGAAAAAGCCTCCTTTCAGCGGGTTGACGCTCTTCCTTGGC
AGCTTCCCTTTGTGGATCGACTGGCAGTGGGAGAGCGTCCCGGAAAGCATGATTTGCATCACCAGGGAGCCTATTATTGC
CTAGATTTCTCCTCGGTATTTGCCGCAACTCCTCTCACGGTTATTCGGAAAAAGCCTAAAGTCGTGCTCGATCTGTGTGC
AGCTCCGGGAGGGAAAAGCCTTTTAGCTTGGCGATTCCTACAGCCCCACTTACTGGTGAGTAACGAAGTGATTGGTAAAC
GGGTGGGAATGCTCATTAGCAATTTGAAACGATGTCAAATTGCACCTGTGCGGGTGTTGAGCGCGGATCCATCAGTACTA
GCTGAAGCGATTCCTGCCAGTGCTGATGTGGTGTTAGTTGATGCCCCTTGTACGGGGCAATCTTTGTTAGCAAAGGGGGA
GTCTGCGCCCGGTTGTTTTCATCCCGCGTCTATCCAACATAATGTGAAGCGACAAAGACGCATCCTCGCTAATGCCTCCC
GTTTGGTGGTGCCGGGAGGATATTTGGCCTATATGACCTGTGCGTTCTCTGAAGCGGAGAATGAGCAGGTCTGTCGCTGG
TTTACTCGCCGCTTCCCCAACTTCGAACCTCAAACGGTTGATGCCTTGTTGGCTTTTCAATCTCACCTAACGGACCTACC
TTGTTATCGGATATGGCCCCAGTCAGGTTTGGGAGCAGGTGCTTTCACCATGTTATTTCGCAATACCCAAGGGACAGCAT
CCATGGCTCAACCTTTAGGGGCATGGCTGGATGAGAATGCTCGTTGGTCTTCTGAATAG

Upstream 100 bases:

>100_bases
TTAATCACCCAAATCATAAACTGTCGCCTTATACTAAGGGGCAGTTTGGGGATATCTTCTCAGCCCTGGCCTCCATGCTA
AGTGATTGACTGTGTCTAAG

Downstream 100 bases:

>100_bases
ATGTTGCCCTGGATGTATTGAGGGGATTGGGGTGCGATCGCAAACGATTCTCTCTGCCAACCTGGCAACAATATCTCCCG
CAAAATGGTCAGATAGGCCA

Product: nol1/nop2/sun family protein

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MLTKFSLKLWPDPQQQQAFIQALTEPQPYSPCIAWIKGRSEKASFQRVDALPWQLPFVDRLAVGERPGKHDLHHQGAYYC
LDFSSVFAATPLTVIRKKPKVVLDLCAAPGGKSLLAWRFLQPHLLVSNEVIGKRVGMLISNLKRCQIAPVRVLSADPSVL
AEAIPASADVVLVDAPCTGQSLLAKGESAPGCFHPASIQHNVKRQRRILANASRLVVPGGYLAYMTCAFSEAENEQVCRW
FTRRFPNFEPQTVDALLAFQSHLTDLPCYRIWPQSGLGAGAFTMLFRNTQGTASMAQPLGAWLDENARWSSE

Sequences:

>Translated_312_residues
MLTKFSLKLWPDPQQQQAFIQALTEPQPYSPCIAWIKGRSEKASFQRVDALPWQLPFVDRLAVGERPGKHDLHHQGAYYC
LDFSSVFAATPLTVIRKKPKVVLDLCAAPGGKSLLAWRFLQPHLLVSNEVIGKRVGMLISNLKRCQIAPVRVLSADPSVL
AEAIPASADVVLVDAPCTGQSLLAKGESAPGCFHPASIQHNVKRQRRILANASRLVVPGGYLAYMTCAFSEAENEQVCRW
FTRRFPNFEPQTVDALLAFQSHLTDLPCYRIWPQSGLGAGAFTMLFRNTQGTASMAQPLGAWLDENARWSSE
>Mature_312_residues
MLTKFSLKLWPDPQQQQAFIQALTEPQPYSPCIAWIKGRSEKASFQRVDALPWQLPFVDRLAVGERPGKHDLHHQGAYYC
LDFSSVFAATPLTVIRKKPKVVLDLCAAPGGKSLLAWRFLQPHLLVSNEVIGKRVGMLISNLKRCQIAPVRVLSADPSVL
AEAIPASADVVLVDAPCTGQSLLAKGESAPGCFHPASIQHNVKRQRRILANASRLVVPGGYLAYMTCAFSEAENEQVCRW
FTRRFPNFEPQTVDALLAFQSHLTDLPCYRIWPQSGLGAGAFTMLFRNTQGTASMAQPLGAWLDENARWSSE

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI11545785, Length=199, Percent_Identity=30.1507537688442, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI87081985, Length=230, Percent_Identity=30.8695652173913, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17536757, Length=148, Percent_Identity=30.4054054054054, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17509941, Length=242, Percent_Identity=30.5785123966942, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19920494, Length=170, Percent_Identity=30.5882352941176, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI22024126, Length=148, Percent_Identity=30.4054054054054, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 34540; Mature: 34540

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKFSLKLWPDPQQQQAFIQALTEPQPYSPCIAWIKGRSEKASFQRVDALPWQLPFVDR
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCHHH
LAVGERPGKHDLHHQGAYYCLDFSSVFAATPLTVIRKKPKVVLDLCAAPGGKSLLAWRFL
HHHCCCCCCCCCCCCCCEEEEEHHHHHHHCCHHHHHCCCCEEEEEECCCCCCCHHHHHHC
QPHLLVSNEVIGKRVGMLISNLKRCQIAPVRVLSADPSVLAEAIPASADVVLVDAPCTGQ
CCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCEEEEECCCCCH
SLLAKGESAPGCFHPASIQHNVKRQRRILANASRLVVPGGYLAYMTCAFSEAENEQVCRW
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCHHHHHHH
FTRRFPNFEPQTVDALLAFQSHLTDLPCYRIWPQSGLGAGAFTMLFRNTQGTASMAQPLG
HHHHCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHEEEEEECCCCCHHHHHHHH
AWLDENARWSSE
HHHCCCCCCCCC
>Mature Secondary Structure
MLTKFSLKLWPDPQQQQAFIQALTEPQPYSPCIAWIKGRSEKASFQRVDALPWQLPFVDR
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCHHH
LAVGERPGKHDLHHQGAYYCLDFSSVFAATPLTVIRKKPKVVLDLCAAPGGKSLLAWRFL
HHHCCCCCCCCCCCCCCEEEEEHHHHHHHCCHHHHHCCCCEEEEEECCCCCCCHHHHHHC
QPHLLVSNEVIGKRVGMLISNLKRCQIAPVRVLSADPSVLAEAIPASADVVLVDAPCTGQ
CCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCEEEEECCCCCH
SLLAKGESAPGCFHPASIQHNVKRQRRILANASRLVVPGGYLAYMTCAFSEAENEQVCRW
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCHHHHHHH
FTRRFPNFEPQTVDALLAFQSHLTDLPCYRIWPQSGLGAGAFTMLFRNTQGTASMAQPLG
HHHHCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHEEEEEECCCCCHHHHHHHH
AWLDENARWSSE
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA