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The map label for this gene is cca [H]

Identifier: 158319038

GI number: 158319038

Start: 8950380

End: 8951906

Strand: Reverse

Name: cca [H]

Synonym: Franean1_7321

Alternate gene names: 158319038

Gene position: 8951906-8950380 (Counterclockwise)

Preceding gene: 158319049

Following gene: 158319036

Centisome position: 99.66

GC content: 73.35

Gene sequence:

>1527_bases
GTGGCTGTGATCAGTCTTGACAATCCCCGGGACTCGGCGGAGCAGGTGGTGAGCGACCTGCTCCGCGTGCCCGCGCCCGC
CGACGAGCTTGGTCGGATCTTCGCCGACCACGGGCATACCCTCCATCTGGTCGGCGGCTCCGTCCGCGACGCGCTGCTCG
GCCGGGCGGGTGACCCGAGCGACACGAGCGCGACCGGTGGCACGGGCGGGGGCGCGCCGGACCTCGACTTCACCACGGAC
GCCCGCCCCGAGCGCGTCCTGGAGATCACCCGGGGCTGGGCCGAGGCGACCTGGGACGCGGGCATCGCGTTCGGCACCGT
CGGCCTGGCCCGCCGCGGGGTGCGCTTCGAGGTCACGACCTACCGCAGCGAGTCGTACGACCGGACGTCCCGCAACCCTG
CCGTCACCTACGGTGAAGGCCTGCAGGAGGACCTCGTCCGCCGCGACTTCACCGTGAACGCGATGGCGGTCTCCGTCCCC
GGGCACGACTTCGTCGACCTCTTCGGCGGGATGGCCGACCTGGCCCGCGGCGTGCTGCGCACCCCCGGCCCCCCGGAGGC
GTCCTTCGACGACGACCCGCTGCGCATCATGCGGGCCGCCCGGTTCGAGGCGGCCCTCGGCCTGACCCCGGTTCCCGAGC
TGGTCGCGGCCATGCGCTCCCGGGCCAGCCGCATCTCGATCGTCTCCCCGGAGCGGGTGCGCGACGAGCTGCGCAAGCTG
ATCGCGAGCCGCGACCCGGCCGCCGGCCTCGAGCGGCTCGTCGAGGTCGGCGTCGCCGACCTGGTGCTCCCCGAGCTGGG
CGCCATGCGGATGGAGATCGACGAGCACCACCAGCACAAGGACGTCTACGCGCACACGATGACCGTGCTGCGCCAGGCGA
TGGCGCTGGAGGACGACGGCCCCGACGAGATCCTGCGCTGGGCCGCGCTGCTGCACGACATCGGCAAGCCGCGCACCCGC
CGGCACATCGCCGGCGGCGGGGTTTCCTTCCACCACCACGAGGTCGTGGGGCGGGACATGACGCGTCGCCGCCTGGTGGC
CCTGCGGTTCCCGAAGGACGTCGTCGACGCCATCTCCTCGCTCGTCTACCTGCACCTGCGCTTCCACGGGTACGGGTCCG
GAGAGTGGACGGACGCCGCTGTCCGTCGCTACGTCCACGACGCCGGGCCGCAGCTCTCCCGGCTGCACAAGCTCGTCCGC
TCGGACTGCACGACCCGCAACCGGCGCAAGGCGGAGAACCTGGCCCGTGCCTACCGGTCGCTCGAGGAGCGGATCGCCGA
CCTCGCCGCCCGCGAGGAGATCGCGGCGATCCGGCCGGAGCTGTCCGGCGACGACATCATGGCCCTGCTGGGCCTGCCGC
CGTCGCGGCTGGTCGGGCAGGCCCGGGCGTACATGCTCGACGTCCGCTTCGAGCGCGGTCTCCTGGGCCGCGAGGCCGCC
GAGGCCGAGCTGTTCCGCTGGGCCCGGGAGAACGACATCGTCATCCCCGGCGAAACTCCCACCCCGGACGAGGCCCCTGA
CGCCTGA

Upstream 100 bases:

>100_bases
CGGGGGACCATACGACGATGGTCCCGCGTCGAGGGTGGCCGGACGCGTGCCAGATAGGCACGGCCACCAGCAACGGTCCG
CTCGCCCGTAATCTGAACAG

Downstream 100 bases:

>100_bases
TCCTGATGCCAGCCCGGGGTTCGTGCCAGCCCGGGGCTCGCTTCGGCGTCACCGGGCCGGGCCCGGCCCGGTGGCTGAGA
CCGGGCCCGCAGGTCGGCCT

Product: polynucleotide adenylyltransferase/metal dependent phosphohydrolase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MAVISLDNPRDSAEQVVSDLLRVPAPADELGRIFADHGHTLHLVGGSVRDALLGRAGDPSDTSATGGTGGGAPDLDFTTD
ARPERVLEITRGWAEATWDAGIAFGTVGLARRGVRFEVTTYRSESYDRTSRNPAVTYGEGLQEDLVRRDFTVNAMAVSVP
GHDFVDLFGGMADLARGVLRTPGPPEASFDDDPLRIMRAARFEAALGLTPVPELVAAMRSRASRISIVSPERVRDELRKL
IASRDPAAGLERLVEVGVADLVLPELGAMRMEIDEHHQHKDVYAHTMTVLRQAMALEDDGPDEILRWAALLHDIGKPRTR
RHIAGGGVSFHHHEVVGRDMTRRRLVALRFPKDVVDAISSLVYLHLRFHGYGSGEWTDAAVRRYVHDAGPQLSRLHKLVR
SDCTTRNRRKAENLARAYRSLEERIADLAAREEIAAIRPELSGDDIMALLGLPPSRLVGQARAYMLDVRFERGLLGREAA
EAELFRWARENDIVIPGETPTPDEAPDA

Sequences:

>Translated_508_residues
MAVISLDNPRDSAEQVVSDLLRVPAPADELGRIFADHGHTLHLVGGSVRDALLGRAGDPSDTSATGGTGGGAPDLDFTTD
ARPERVLEITRGWAEATWDAGIAFGTVGLARRGVRFEVTTYRSESYDRTSRNPAVTYGEGLQEDLVRRDFTVNAMAVSVP
GHDFVDLFGGMADLARGVLRTPGPPEASFDDDPLRIMRAARFEAALGLTPVPELVAAMRSRASRISIVSPERVRDELRKL
IASRDPAAGLERLVEVGVADLVLPELGAMRMEIDEHHQHKDVYAHTMTVLRQAMALEDDGPDEILRWAALLHDIGKPRTR
RHIAGGGVSFHHHEVVGRDMTRRRLVALRFPKDVVDAISSLVYLHLRFHGYGSGEWTDAAVRRYVHDAGPQLSRLHKLVR
SDCTTRNRRKAENLARAYRSLEERIADLAAREEIAAIRPELSGDDIMALLGLPPSRLVGQARAYMLDVRFERGLLGREAA
EAELFRWARENDIVIPGETPTPDEAPDA
>Mature_507_residues
AVISLDNPRDSAEQVVSDLLRVPAPADELGRIFADHGHTLHLVGGSVRDALLGRAGDPSDTSATGGTGGGAPDLDFTTDA
RPERVLEITRGWAEATWDAGIAFGTVGLARRGVRFEVTTYRSESYDRTSRNPAVTYGEGLQEDLVRRDFTVNAMAVSVPG
HDFVDLFGGMADLARGVLRTPGPPEASFDDDPLRIMRAARFEAALGLTPVPELVAAMRSRASRISIVSPERVRDELRKLI
ASRDPAAGLERLVEVGVADLVLPELGAMRMEIDEHHQHKDVYAHTMTVLRQAMALEDDGPDEILRWAALLHDIGKPRTRR
HIAGGGVSFHHHEVVGRDMTRRRLVALRFPKDVVDAISSLVYLHLRFHGYGSGEWTDAAVRRYVHDAGPQLSRLHKLVRS
DCTTRNRRKAENLARAYRSLEERIADLAAREEIAAIRPELSGDDIMALLGLPPSRLVGQARAYMLDVRFERGLLGREAAE
AELFRWARENDIVIPGETPTPDEAPDA

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=277, Percent_Identity=27.0758122743682, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1789436, Length=341, Percent_Identity=28.7390029325513, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87081691, Length=220, Percent_Identity=31.3636363636364, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321016, Length=236, Percent_Identity=30.0847457627119, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 55891; Mature: 55760

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVISLDNPRDSAEQVVSDLLRVPAPADELGRIFADHGHTLHLVGGSVRDALLGRAGDPS
CEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCC
DTSATGGTGGGAPDLDFTTDARPERVLEITRGWAEATWDAGIAFGTVGLARRGVRFEVTT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEE
YRSESYDRTSRNPAVTYGEGLQEDLVRRDFTVNAMAVSVPGHDFVDLFGGMADLARGVLR
ECCCCCCCCCCCCCEECCCCHHHHHHHCCCEEEEEEEECCCCHHHHHHCCHHHHHHHHHC
TPGPPEASFDDDPLRIMRAARFEAALGLTPVPELVAAMRSRASRISIVSPERVRDELRKL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEECCCHHHHHHHHHHH
IASRDPAAGLERLVEVGVADLVLPELGAMRMEIDEHHQHKDVYAHTMTVLRQAMALEDDG
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PDEILRWAALLHDIGKPRTRRHIAGGGVSFHHHEVVGRDMTRRRLVALRFPKDVVDAISS
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHCHHHHHHHHHEECCHHHHHHHHH
LVYLHLRFHGYGSGEWTDAAVRRYVHDAGPQLSRLHKLVRSDCTTRNRRKAENLARAYRS
HHHHHHEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LEERIADLAAREEIAAIRPELSGDDIMALLGLPPSRLVGQARAYMLDVRFERGLLGREAA
HHHHHHHHHHHHHHHHHCCCCCCCCEEHHHCCCHHHHHHHHHHHHEEHHHHCCCCCCHHH
EAELFRWARENDIVIPGETPTPDEAPDA
HHHHHHHHCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
AVISLDNPRDSAEQVVSDLLRVPAPADELGRIFADHGHTLHLVGGSVRDALLGRAGDPS
EEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCC
DTSATGGTGGGAPDLDFTTDARPERVLEITRGWAEATWDAGIAFGTVGLARRGVRFEVTT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEE
YRSESYDRTSRNPAVTYGEGLQEDLVRRDFTVNAMAVSVPGHDFVDLFGGMADLARGVLR
ECCCCCCCCCCCCCEECCCCHHHHHHHCCCEEEEEEEECCCCHHHHHHCCHHHHHHHHHC
TPGPPEASFDDDPLRIMRAARFEAALGLTPVPELVAAMRSRASRISIVSPERVRDELRKL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEECCCHHHHHHHHHHH
IASRDPAAGLERLVEVGVADLVLPELGAMRMEIDEHHQHKDVYAHTMTVLRQAMALEDDG
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PDEILRWAALLHDIGKPRTRRHIAGGGVSFHHHEVVGRDMTRRRLVALRFPKDVVDAISS
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHCHHHHHHHHHEECCHHHHHHHHH
LVYLHLRFHGYGSGEWTDAAVRRYVHDAGPQLSRLHKLVRSDCTTRNRRKAENLARAYRS
HHHHHHEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LEERIADLAAREEIAAIRPELSGDDIMALLGLPPSRLVGQARAYMLDVRFERGLLGREAA
HHHHHHHHHHHHHHHHHCCCCCCCCEEHHHCCCHHHHHHHHHHHHEEHHHHCCCCCCHHH
EAELFRWARENDIVIPGETPTPDEAPDA
HHHHHHHHCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA