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The map label for this gene is levB [H]

Identifier: 158318763

GI number: 158318763

Start: 8583805

End: 8585850

Strand: Reverse

Name: levB [H]

Synonym: Franean1_7034

Alternate gene names: 158318763

Gene position: 8585850-8583805 (Counterclockwise)

Preceding gene: 158318764

Following gene: 158318762

Centisome position: 95.59

GC content: 62.66

Gene sequence:

>2046_bases
ATGAATTTACGCATCAGACGAAGCGCGGGAAACAAGCATTCCGTGGTCAGGCGCCCCGTTACTCTGACGGCGGTTCTGAC
GGCGGCCACGGCGGCAGCCAGCATATTATTAGGCCTGCCGGCGCCCACTCCGGCGTCGGCAGTCGGTCCGAGTTACCGGG
ACACCTACCACTTCACCGTGCCCGACCACTGGAAGAACGACCCGCAACGTCCGGTGTACGTGAACGGCAAGTTCTATTAC
TACTACCTCTACAACGCCGATTACGACGCCAACCCGACGGCCAACTACGGCACCGAGTGGCGGCTCGCGACCAGCTACGA
CGGCGTCGTGTTCGCCGATCAGGGGGTTGCGGCACCGAAGAAGACGAACGCCAACTATGATCTGTGGTCGGGCTCAACGG
TGGTCGACACCGCCAATACAGCCGGGTTCGGCGCAGGCGCCGTCGTGATGCTCGTGACCCAGATGGACCATCCGACCGAG
GCGCAGAAGCTCAACGCATCCGGTCCGCAGGCCCAGTTCCTCTGGTATTCAACGAACGGCGGCCGCAACTTCACACCGTA
CGGCGAGGCGCCGGTCATCGCGAACGGCGGTCGGGCTGACTTCCGGGATCCGAAGGTCCTGTGGGATGCTGACCGCAACC
GCTGGGTCGCGCTCATCGCCGAAGGCCAGAAGATCGGTTTCTACACCTCGGCCAACCTCAAGGACTGGACCCGGGTCTCC
GAGTACACCAACAGCGGCCTCGGTATTCTGGAGTGCCCCGACCTGTTCAAGATGCGTGCCGATGACGGCACCACCCACTG
GGTAATGGGCATGAGCGCAAACGGTTACCTCACCGGCGCACCCAGCACGTACGCCTACTGGACAGGAAGTTTCGACGGCA
CGACATTCACACCCGACACGGCGGAGCCGCAGTGGGCCGACCACGGATTCGACTGGTACGGTGCGGTCACCTGGGAGGAC
CCGACGGCACCACTCGACAAGCGCTACGCGATCGGATGGATGAACAACTGGTCGTACCCGCACTCGACGCCGACGTGGCC
GAACGACGGCTTCAATGGAACCGACTCGATCACCCGCCAACTGGCACTGAAGAAATACGGATCGACATACAGCCTAACAT
CCCAGCCAGTTGCTGCACTGAACAATATTGCGACACAGACAACGAACCTCGGTACGTTCTCGGTGAACGGCACCGTGCCG
CTCGCCTACAGTGGTACCTCGTACCAGCTCGAGACGACGGTGACCTGGAGCACTGCACAGAACATCGGTCTTGGGCTGCG
CAGGTCGAACGACGGTACCCGCCACGCCGACGTGGGCGTGCACGACACGTACAGTTATCTTAACCGAGGTGGTACCACAA
ATCCGGACACTTCTGGACAGAAGCTTGAAAGTCGCGCTCCGTTCGACATGAGCGCCCAAACCGTGCACCTGCGTATCCTC
GTCGACCGCACCACGATCGAAGTGTTTGTCGACGACGGGCGCTTCGTGCACTCGAGCCAGGTCTTCCCCGACCCCGCCGA
TGCCGGCATCGCCCTGTACTCGCTCGGTGGGACAGCGACCTTCTCCAACGTCACGATCACCGAATTCGGTAGCGTCGTCC
AGAGGCCAGCACGACTGATCGCGGACTTCGAGGGCTCGACCTGGGGCAACGGATGGACCGCGACGGGCTCCTTCGCCTCC
GCCGCGCCGACCGTGGCATCGCTGCCCGGACAGGTTGGCGCCAAGGTCGCCGACACCTACGTCGGCGGCGGTGACCCGGC
GACCGGCACGATCACCTCCCCGCCGTTCACCATCGATCGCAACCATCTCCACTTCTCCATAGCGGGCGGCAACCATCCCC
TTGGCGCGGAACCGGCCACGTCCGTCCAACTGCTCGTCGGCGGACAACCTGTCCTCACGTCAACCGGGGATAATTCGAGT
ACCCTGCGACATGTCGAATGGGATGTTACTGCCTATGCCGGACAGGCCGCACAATTCCAGATCCTTGACGACGCGACTGG
AACTTGGGGACACCTCGTGGTCGACCAGGTCGTACTGAGCGACTGA

Upstream 100 bases:

>100_bases
TCGAATGGCACGACGACGAGTTGGTCGTCGCCACGCCGTTGGCGGACAAGAAAAGCAAGAGCTTTTTGGCGCCAGGTCGA
TCACATTTAAGGAGTTAAAG

Downstream 100 bases:

>100_bases
GCGGCTGAGTCAGCTCGGTGGACCCGGGCCGATGCGTTGTGGTTGCCGGAAACCGGGTTCGGGTCATGCGGCCTGCCGGT
ATTCGTGGATGAGCCCGCCG

Product: levanase

Products: NA

Alternate protein names: 2,6-beta-fructan 6-levanbiohydrolase; Endo-levanase [H]

Number of amino acids: Translated: 681; Mature: 681

Protein sequence:

>681_residues
MNLRIRRSAGNKHSVVRRPVTLTAVLTAATAAASILLGLPAPTPASAVGPSYRDTYHFTVPDHWKNDPQRPVYVNGKFYY
YYLYNADYDANPTANYGTEWRLATSYDGVVFADQGVAAPKKTNANYDLWSGSTVVDTANTAGFGAGAVVMLVTQMDHPTE
AQKLNASGPQAQFLWYSTNGGRNFTPYGEAPVIANGGRADFRDPKVLWDADRNRWVALIAEGQKIGFYTSANLKDWTRVS
EYTNSGLGILECPDLFKMRADDGTTHWVMGMSANGYLTGAPSTYAYWTGSFDGTTFTPDTAEPQWADHGFDWYGAVTWED
PTAPLDKRYAIGWMNNWSYPHSTPTWPNDGFNGTDSITRQLALKKYGSTYSLTSQPVAALNNIATQTTNLGTFSVNGTVP
LAYSGTSYQLETTVTWSTAQNIGLGLRRSNDGTRHADVGVHDTYSYLNRGGTTNPDTSGQKLESRAPFDMSAQTVHLRIL
VDRTTIEVFVDDGRFVHSSQVFPDPADAGIALYSLGGTATFSNVTITEFGSVVQRPARLIADFEGSTWGNGWTATGSFAS
AAPTVASLPGQVGAKVADTYVGGGDPATGTITSPPFTIDRNHLHFSIAGGNHPLGAEPATSVQLLVGGQPVLTSTGDNSS
TLRHVEWDVTAYAGQAAQFQILDDATGTWGHLVVDQVVLSD

Sequences:

>Translated_681_residues
MNLRIRRSAGNKHSVVRRPVTLTAVLTAATAAASILLGLPAPTPASAVGPSYRDTYHFTVPDHWKNDPQRPVYVNGKFYY
YYLYNADYDANPTANYGTEWRLATSYDGVVFADQGVAAPKKTNANYDLWSGSTVVDTANTAGFGAGAVVMLVTQMDHPTE
AQKLNASGPQAQFLWYSTNGGRNFTPYGEAPVIANGGRADFRDPKVLWDADRNRWVALIAEGQKIGFYTSANLKDWTRVS
EYTNSGLGILECPDLFKMRADDGTTHWVMGMSANGYLTGAPSTYAYWTGSFDGTTFTPDTAEPQWADHGFDWYGAVTWED
PTAPLDKRYAIGWMNNWSYPHSTPTWPNDGFNGTDSITRQLALKKYGSTYSLTSQPVAALNNIATQTTNLGTFSVNGTVP
LAYSGTSYQLETTVTWSTAQNIGLGLRRSNDGTRHADVGVHDTYSYLNRGGTTNPDTSGQKLESRAPFDMSAQTVHLRIL
VDRTTIEVFVDDGRFVHSSQVFPDPADAGIALYSLGGTATFSNVTITEFGSVVQRPARLIADFEGSTWGNGWTATGSFAS
AAPTVASLPGQVGAKVADTYVGGGDPATGTITSPPFTIDRNHLHFSIAGGNHPLGAEPATSVQLLVGGQPVLTSTGDNSS
TLRHVEWDVTAYAGQAAQFQILDDATGTWGHLVVDQVVLSD
>Mature_681_residues
MNLRIRRSAGNKHSVVRRPVTLTAVLTAATAAASILLGLPAPTPASAVGPSYRDTYHFTVPDHWKNDPQRPVYVNGKFYY
YYLYNADYDANPTANYGTEWRLATSYDGVVFADQGVAAPKKTNANYDLWSGSTVVDTANTAGFGAGAVVMLVTQMDHPTE
AQKLNASGPQAQFLWYSTNGGRNFTPYGEAPVIANGGRADFRDPKVLWDADRNRWVALIAEGQKIGFYTSANLKDWTRVS
EYTNSGLGILECPDLFKMRADDGTTHWVMGMSANGYLTGAPSTYAYWTGSFDGTTFTPDTAEPQWADHGFDWYGAVTWED
PTAPLDKRYAIGWMNNWSYPHSTPTWPNDGFNGTDSITRQLALKKYGSTYSLTSQPVAALNNIATQTTNLGTFSVNGTVP
LAYSGTSYQLETTVTWSTAQNIGLGLRRSNDGTRHADVGVHDTYSYLNRGGTTNPDTSGQKLESRAPFDMSAQTVHLRIL
VDRTTIEVFVDDGRFVHSSQVFPDPADAGIALYSLGGTATFSNVTITEFGSVVQRPARLIADFEGSTWGNGWTATGSFAS
AAPTVASLPGQVGAKVADTYVGGGDPATGTITSPPFTIDRNHLHFSIAGGNHPLGAEPATSVQLLVGGQPVLTSTGDNSS
TLRHVEWDVTAYAGQAAQFQILDDATGTWGHLVVDQVVLSD

Specific function: Catalyzes the degradation of levan mainly into levanbiose (difructose). Is not active on sucrose [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=510, Percent_Identity=29.0196078431373, Blast_Score=137, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR013189
- InterPro:   IPR013148 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.64 [H]

Molecular weight: Translated: 73395; Mature: 73395

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRIRRSAGNKHSVVRRPVTLTAVLTAATAAASILLGLPAPTPASAVGPSYRDTYHFTV
CCEEEECCCCCCCCEEECCEEEEHHHHHHHHHEEEEEECCCCCCHHHCCCCCCCEEEEEC
PDHWKNDPQRPVYVNGKFYYYYLYNADYDANPTANYGTEWRLATSYDGVVFADQGVAAPK
CHHCCCCCCCCEEECCEEEEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCC
KTNANYDLWSGSTVVDTANTAGFGAGAVVMLVTQMDHPTEAQKLNASGPQAQFLWYSTNG
CCCCCEEEECCCEEEECCCCCCCCCCEEEEEEEECCCCCHHHHCCCCCCCEEEEEEECCC
GRNFTPYGEAPVIANGGRADFRDPKVLWDADRNRWVALIAEGQKIGFYTSANLKDWTRVS
CCCCCCCCCCCEEECCCCCCCCCCCEEEECCCCCEEEEEECCCEEEEEEECCCCHHHHHH
EYTNSGLGILECPDLFKMRADDGTTHWVMGMSANGYLTGAPSTYAYWTGSFDGTTFTPDT
HHHCCCCEEEECCHHHEEECCCCCEEEEEEECCCCEEECCCCEEEEEECCCCCCEECCCC
AEPQWADHGFDWYGAVTWEDPTAPLDKRYAIGWMNNWSYPHSTPTWPNDGFNGTDSITRQ
CCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
LALKKYGSTYSLTSQPVAALNNIATQTTNLGTFSVNGTVPLAYSGTSYQLETTVTWSTAQ
HHHHHCCCEEEECCCCHHHHHHHHHCCCCCEEEEECCCEEEEECCCCEEEEEEEEEECCC
NIGLGLRRSNDGTRHADVGVHDTYSYLNRGGTTNPDTSGQKLESRAPFDMSAQTVHLRIL
CCCEEEEECCCCCEECCCCCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCEEEEEEEE
VDRTTIEVFVDDGRFVHSSQVFPDPADAGIALYSLGGTATFSNVTITEFGSVVQRPARLI
EEEEEEEEEECCCEEEECCCCCCCCCCCCEEEEECCCCEEECCEEHHHHHHHHHHHHHHE
ADFEGSTWGNGWTATGSFASAAPTVASLPGQVGAKVADTYVGGGDPATGTITSPPFTIDR
EECCCCCCCCCEEECCCCCCCCCHHHHCCCHHCCEEEHEECCCCCCCCCCCCCCCEEEEC
NHLHFSIAGGNHPLGAEPATSVQLLVGGQPVLTSTGDNSSTLRHVEWDVTAYAGQAAQFQ
CEEEEEECCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCEEEEEEEEEEEECCCCEEEE
ILDDATGTWGHLVVDQVVLSD
EEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNLRIRRSAGNKHSVVRRPVTLTAVLTAATAAASILLGLPAPTPASAVGPSYRDTYHFTV
CCEEEECCCCCCCCEEECCEEEEHHHHHHHHHEEEEEECCCCCCHHHCCCCCCCEEEEEC
PDHWKNDPQRPVYVNGKFYYYYLYNADYDANPTANYGTEWRLATSYDGVVFADQGVAAPK
CHHCCCCCCCCEEECCEEEEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCC
KTNANYDLWSGSTVVDTANTAGFGAGAVVMLVTQMDHPTEAQKLNASGPQAQFLWYSTNG
CCCCCEEEECCCEEEECCCCCCCCCCEEEEEEEECCCCCHHHHCCCCCCCEEEEEEECCC
GRNFTPYGEAPVIANGGRADFRDPKVLWDADRNRWVALIAEGQKIGFYTSANLKDWTRVS
CCCCCCCCCCCEEECCCCCCCCCCCEEEECCCCCEEEEEECCCEEEEEEECCCCHHHHHH
EYTNSGLGILECPDLFKMRADDGTTHWVMGMSANGYLTGAPSTYAYWTGSFDGTTFTPDT
HHHCCCCEEEECCHHHEEECCCCCEEEEEEECCCCEEECCCCEEEEEECCCCCCEECCCC
AEPQWADHGFDWYGAVTWEDPTAPLDKRYAIGWMNNWSYPHSTPTWPNDGFNGTDSITRQ
CCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
LALKKYGSTYSLTSQPVAALNNIATQTTNLGTFSVNGTVPLAYSGTSYQLETTVTWSTAQ
HHHHHCCCEEEECCCCHHHHHHHHHCCCCCEEEEECCCEEEEECCCCEEEEEEEEEECCC
NIGLGLRRSNDGTRHADVGVHDTYSYLNRGGTTNPDTSGQKLESRAPFDMSAQTVHLRIL
CCCEEEEECCCCCEECCCCCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCEEEEEEEE
VDRTTIEVFVDDGRFVHSSQVFPDPADAGIALYSLGGTATFSNVTITEFGSVVQRPARLI
EEEEEEEEEECCCEEEECCCCCCCCCCCCEEEEECCCCEEECCEEHHHHHHHHHHHHHHE
ADFEGSTWGNGWTATGSFASAAPTVASLPGQVGAKVADTYVGGGDPATGTITSPPFTIDR
EECCCCCCCCCEEECCCCCCCCCHHHHCCCHHCCEEEHEECCCCCCCCCCCCCCCEEEEC
NHLHFSIAGGNHPLGAEPATSVQLLVGGQPVLTSTGDNSSTLRHVEWDVTAYAGQAAQFQ
CEEEEEECCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCEEEEEEEEEEEECCCCEEEE
ILDDATGTWGHLVVDQVVLSD
EEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]