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The map label for this gene is purR [H]

Identifier: 158318756

GI number: 158318756

Start: 8570625

End: 8571686

Strand: Direct

Name: purR [H]

Synonym: Franean1_7027

Alternate gene names: 158318756

Gene position: 8570625-8571686 (Clockwise)

Preceding gene: 158318747

Following gene: 158318757

Centisome position: 95.42

GC content: 66.76

Gene sequence:

>1062_bases
ATGGTCAAGATGGCGGACGTGGCGAAGATCGCGGGCGTCTCCGTGTCCACGGTCTCGCACGTCCTCAACGACACGCGGGC
GGTGCGGGCCGAGACCCGCGCGGCCGTGCTCGCAGCCATCGAACAGACCGGTTACACACAGAACACGATCGCGCGGGCGC
TCGTCACGGCCAGCAGTGGCCTGATCGGCCTATCGATCCCGGTGATCACCAACCCCTACTTCACCGACGTGATCCACGCG
ATCGAGACGAACGTGTCGGCCACCAGCTACACCCTGATACTCGCCGACTCGCGCGAGGACCCCGAGCATGAGCTCCGGGC
CCTCCGGGCGCTCCTTCAACGTCGGATCGACGGGTTGATCATCGTACCGGCCGGCGGTGACAATCAGCTGGCCTTGGAGC
ACCTCGCCGCGAGCTCCGTGCCGACGGTACTCATCGACCGGCTGGCGTCAGCCGACTTCGATCAGGTCGGACCAGACAAC
GAGGAGCCGACCGCTCAGCTCGTCGAGCACCTCGCAGACCTGGGACACCACCGAATTGGCATGGTCGCCGGCCTGAGCGG
TCTCACCACCACCCGCGAGCGACTGGCCGGCTACCGGCGGGGGCTAGACCGACACGGACTGCCTTATGACGAGAAACTAG
TTGCCGGCGGCGGCTCCCAGATCGAGGGTGCCAGGGCAGCCGCCCACACCTTGCTAGCGCTGCCGAATCCGCCGACCGCG
CTGGTCGTCGCCAACAACCTCATGACGATCGGGACCATGCACGCACTACGGGAGACAGGCCGGCGGGTGCCCGACGACAT
CGCGCTTACCTGCTTCGACGACTTCGAATGGGCCGACCTCTTCTCGCCCCGGCTGACCACCATGGCCCAGCCACGCGTTG
AGTTGGGCAACGTCGCGGTGGAGTTGCTGATGCGACGGCTCGCCGACCCCACGGTGCCTCCGCGTACGGTACGACTTGCC
CCCCAGTTCATGCACCGCGACTCCTGTGGCTGCACTCCCGAACAACGAATGGCGACGGGCCAGGACAAGGAGGTCGGCAG
CCCTTCCAGCCCGCCAGTATGA

Upstream 100 bases:

>100_bases
CGGGGTTGCGCCGGAATGTAGCTCGGGTCACAGTAGGAGCGCAAGCGCTTGCGCGAACGTTTGCCCAACTTCTCGTCGGG
ACGTCCGGTAGGGAGATCGC

Downstream 100 bases:

>100_bases
CTCAGGAGGGATCACCTGATACACCGTTGACCCGTCCCTGATTCGCCTATGGATGGTCGTGGCGTGAGTACGCCGGCTTC
CGGCGCTGCCCGCTCTTTGC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MVKMADVAKIAGVSVSTVSHVLNDTRAVRAETRAAVLAAIEQTGYTQNTIARALVTASSGLIGLSIPVITNPYFTDVIHA
IETNVSATSYTLILADSREDPEHELRALRALLQRRIDGLIIVPAGGDNQLALEHLAASSVPTVLIDRLASADFDQVGPDN
EEPTAQLVEHLADLGHHRIGMVAGLSGLTTTRERLAGYRRGLDRHGLPYDEKLVAGGGSQIEGARAAAHTLLALPNPPTA
LVVANNLMTIGTMHALRETGRRVPDDIALTCFDDFEWADLFSPRLTTMAQPRVELGNVAVELLMRRLADPTVPPRTVRLA
PQFMHRDSCGCTPEQRMATGQDKEVGSPSSPPV

Sequences:

>Translated_353_residues
MVKMADVAKIAGVSVSTVSHVLNDTRAVRAETRAAVLAAIEQTGYTQNTIARALVTASSGLIGLSIPVITNPYFTDVIHA
IETNVSATSYTLILADSREDPEHELRALRALLQRRIDGLIIVPAGGDNQLALEHLAASSVPTVLIDRLASADFDQVGPDN
EEPTAQLVEHLADLGHHRIGMVAGLSGLTTTRERLAGYRRGLDRHGLPYDEKLVAGGGSQIEGARAAAHTLLALPNPPTA
LVVANNLMTIGTMHALRETGRRVPDDIALTCFDDFEWADLFSPRLTTMAQPRVELGNVAVELLMRRLADPTVPPRTVRLA
PQFMHRDSCGCTPEQRMATGQDKEVGSPSSPPV
>Mature_353_residues
MVKMADVAKIAGVSVSTVSHVLNDTRAVRAETRAAVLAAIEQTGYTQNTIARALVTASSGLIGLSIPVITNPYFTDVIHA
IETNVSATSYTLILADSREDPEHELRALRALLQRRIDGLIIVPAGGDNQLALEHLAASSVPTVLIDRLASADFDQVGPDN
EEPTAQLVEHLADLGHHRIGMVAGLSGLTTTRERLAGYRRGLDRHGLPYDEKLVAGGGSQIEGARAAAHTLLALPNPPTA
LVVANNLMTIGTMHALRETGRRVPDDIALTCFDDFEWADLFSPRLTTMAQPRVELGNVAVELLMRRLADPTVPPRTVRLA
PQFMHRDSCGCTPEQRMATGQDKEVGSPSSPPV

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=333, Percent_Identity=35.1351351351351, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=32.5373134328358, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1790369, Length=317, Percent_Identity=30.5993690851735, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789068, Length=335, Percent_Identity=31.9402985074627, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1786540, Length=307, Percent_Identity=32.5732899022801, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788474, Length=331, Percent_Identity=29.3051359516616, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789202, Length=334, Percent_Identity=29.6407185628743, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=28.3489096573209, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787906, Length=327, Percent_Identity=29.3577981651376, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI48994940, Length=322, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786268, Length=317, Percent_Identity=25.2365930599369, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790715, Length=327, Percent_Identity=25.3822629969419, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790689, Length=316, Percent_Identity=25, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37855; Mature: 37855

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKMADVAKIAGVSVSTVSHVLNDTRAVRAETRAAVLAAIEQTGYTQNTIARALVTASSG
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
LIGLSIPVITNPYFTDVIHAIETNVSATSYTLILADSREDPEHELRALRALLQRRIDGLI
EEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEE
IVPAGGDNQLALEHLAASSVPTVLIDRLASADFDQVGPDNEEPTAQLVEHLADLGHHRIG
EEECCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHCCHHHH
MVAGLSGLTTTRERLAGYRRGLDRHGLPYDEKLVAGGGSQIEGARAAAHTLLALPNPPTA
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHEEECCCCCEE
LVVANNLMTIGTMHALRETGRRVPDDIALTCFDDFEWADLFSPRLTTMAQPRVELGNVAV
EEEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCHHHHHCCCCHHHHHHHH
ELLMRRLADPTVPPRTVRLAPQFMHRDSCGCTPEQRMATGQDKEVGSPSSPPV
HHHHHHHCCCCCCCCEEECCHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVKMADVAKIAGVSVSTVSHVLNDTRAVRAETRAAVLAAIEQTGYTQNTIARALVTASSG
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
LIGLSIPVITNPYFTDVIHAIETNVSATSYTLILADSREDPEHELRALRALLQRRIDGLI
EEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEE
IVPAGGDNQLALEHLAASSVPTVLIDRLASADFDQVGPDNEEPTAQLVEHLADLGHHRIG
EEECCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHCCHHHH
MVAGLSGLTTTRERLAGYRRGLDRHGLPYDEKLVAGGGSQIEGARAAAHTLLALPNPPTA
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHEEECCCCCEE
LVVANNLMTIGTMHALRETGRRVPDDIALTCFDDFEWADLFSPRLTTMAQPRVELGNVAV
EEEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCHHHHHCCCCHHHHHHHH
ELLMRRLADPTVPPRTVRLAPQFMHRDSCGCTPEQRMATGQDKEVGSPSSPPV
HHHHHHHCCCCCCCCEEECCHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA