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The map label for this gene is emrB [H]

Identifier: 158318697

GI number: 158318697

Start: 8490718

End: 8492364

Strand: Direct

Name: emrB [H]

Synonym: Franean1_6965

Alternate gene names: 158318697

Gene position: 8490718-8492364 (Clockwise)

Preceding gene: 158318688

Following gene: 158318698

Centisome position: 94.53

GC content: 74.86

Gene sequence:

>1647_bases
GTGCGCGCGGAGCGAGACATCATCCAGCCGAGGACGTCCGCTGACGAGGCCGCTGACGAGGCCGCTGACGAGGCGGCGGG
CGAGGCGGCGGACGAAGTCGCGGAGCCGAAGCCGCCGGGCCCCACCGCGGTGGCTCGGCCGTCCGGCGGAGGGGGGCCGC
ACCCCCGCCCGGGCCTGTTGCTGGGCGTGCTCGTCTACTGCGGGCTCGTGATCGCCGTCATCGGGACGCTGGGCACACCA
CTGATCCCGACGATCGCGGCCACCCAGCACGTCTCGCTGGACAGCGCCCAGTGGCTGCTCACCCTCACACTGCTGACCGG
CGCCGCGTCCACTCCGCTGATCGGGCGTCTCGGCGACGGGCCGCACCGGCGGACCGTGCTGCTCGCCGGCCTGGGCGCCG
TCGCGGTCGGCTCCGTTCTCTCCGCCACCGCCAACGGCTTCGCGCAGCTGCTGGTCGGGCGGGGCCTGATGGGCGTCGGG
ATGGGCCTGATGCCGCTGGCGCTGGCGCTCGCCCGTGACCTGCTGCCCCCGCACAAAATGGCTCCGGGGGTCGCCGCCCT
GTCCATCACGGTCGCGACCGGCGCCGGCCTGGGCTACCCACTCAGCGGGCTACTCGCGGACACGTTCGACTACCACGCCG
GCTTCTGGGTCGCCGCCGCGCTGGCGGCTGCCGGGATGGCGGCCGTGCTCGTGGCCGTCCCGGGCCGGGCGAGCGCGCGG
ACCTCCCATGGGCGGGTCGACCTGCGGGGCGCGGTGCTGTTCGCCGCGGCGCTGAGCCCGGTGCTGCTCGCGCTGAGCGA
GGGCGAGTCGTGGGGCTGGCTGTCACCGGCGGTCATCGCACTGCTCGTGGGTGGCATCGGCTGCGGGGTGGCCTGGGCCC
TGGTCGAGCTGCGGACGGACAACCCGCTGATAGAGCTGAAGTACCTGGCGGCGCGGCCGGTGCTCATCGCCGACATGTGC
GCGGCGCTGGCCGGCTTCGGCATGTTCAACGCGATGACGTTGATCAACCGGCTGGCGCAGGCCCCGACCTCGACCGGCTA
CGGCTTCGGCGCGTCACCGGCCGTGCTCGGCCTGGTGATCCTGCCCCTGTCGGCCGGGACGGTGCTGGCCAGCCGGTGGT
CGCGCTGGCTGGGCCCGCGCACCGGCGGTGGGCGGGGCCTGCTGTTGTGCGGCCTGATGGCGGTCGCCCTCGCCCTGTTC
GGCCTGGCCGTCAGCCACGACCACCTCGTCGAGCTGGGCGCGGCCACGTTCCTGTTCGGGGTCGGCATCGGACTGGCCTT
CGCCGCGATGCCCGCCCTGATCATGGGAGCCGTCCCGCCGCACGAGACGGGCAGCGCGACCAGCTTCAACCAGGTGCTGC
GCACGGCTGGCGGCTCGGTGGGAAGCGCGCTCGGCGCGGCGCTGCTCGCCGCCCACACACCGGCCGGTTCGGTCGAGCCG
ACCAACAGCGGGTACACGGTGGCGTTCGTCGTGGCCGGCGGGGTCTGCGCGCTGGCCGCGCTCGCGGCGCTGGCCCTGCC
CGGCACCTCGTCCGCGGCGACCCGCCCGTTGGGGGCGGCGCGCCGGACGGAACTGGAGACACTGGAGGAGGAATCGGCGG
GCTCCACCGCCGCGGGACTCGTCATGGCGGAGGATGTCCGGTCATGA

Upstream 100 bases:

>100_bases
ACACCGAGCGTGACCTGCGGGTACAGGTTGCGCCAGCTCTTGTCTTCGTTCGATGTCTTCTTATGATGACATCAAGCGAT
GACCAGACTGGGGGCATAAC

Downstream 100 bases:

>100_bases
ACACCACTCGGCGGCGCGACGCTGCGCGCAGCCGCCAGGCGCTGCTGGCCGCGGCGGCCGACCTGTTCGCCGAGCGCGGC
TACGCGGGAACCGCGATCCG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MRAERDIIQPRTSADEAADEAADEAAGEAADEVAEPKPPGPTAVARPSGGGGPHPRPGLLLGVLVYCGLVIAVIGTLGTP
LIPTIAATQHVSLDSAQWLLTLTLLTGAASTPLIGRLGDGPHRRTVLLAGLGAVAVGSVLSATANGFAQLLVGRGLMGVG
MGLMPLALALARDLLPPHKMAPGVAALSITVATGAGLGYPLSGLLADTFDYHAGFWVAAALAAAGMAAVLVAVPGRASAR
TSHGRVDLRGAVLFAAALSPVLLALSEGESWGWLSPAVIALLVGGIGCGVAWALVELRTDNPLIELKYLAARPVLIADMC
AALAGFGMFNAMTLINRLAQAPTSTGYGFGASPAVLGLVILPLSAGTVLASRWSRWLGPRTGGGRGLLLCGLMAVALALF
GLAVSHDHLVELGAATFLFGVGIGLAFAAMPALIMGAVPPHETGSATSFNQVLRTAGGSVGSALGAALLAAHTPAGSVEP
TNSGYTVAFVVAGGVCALAALAALALPGTSSAATRPLGAARRTELETLEEESAGSTAAGLVMAEDVRS

Sequences:

>Translated_548_residues
MRAERDIIQPRTSADEAADEAADEAAGEAADEVAEPKPPGPTAVARPSGGGGPHPRPGLLLGVLVYCGLVIAVIGTLGTP
LIPTIAATQHVSLDSAQWLLTLTLLTGAASTPLIGRLGDGPHRRTVLLAGLGAVAVGSVLSATANGFAQLLVGRGLMGVG
MGLMPLALALARDLLPPHKMAPGVAALSITVATGAGLGYPLSGLLADTFDYHAGFWVAAALAAAGMAAVLVAVPGRASAR
TSHGRVDLRGAVLFAAALSPVLLALSEGESWGWLSPAVIALLVGGIGCGVAWALVELRTDNPLIELKYLAARPVLIADMC
AALAGFGMFNAMTLINRLAQAPTSTGYGFGASPAVLGLVILPLSAGTVLASRWSRWLGPRTGGGRGLLLCGLMAVALALF
GLAVSHDHLVELGAATFLFGVGIGLAFAAMPALIMGAVPPHETGSATSFNQVLRTAGGSVGSALGAALLAAHTPAGSVEP
TNSGYTVAFVVAGGVCALAALAALALPGTSSAATRPLGAARRTELETLEEESAGSTAAGLVMAEDVRS
>Mature_548_residues
MRAERDIIQPRTSADEAADEAADEAAGEAADEVAEPKPPGPTAVARPSGGGGPHPRPGLLLGVLVYCGLVIAVIGTLGTP
LIPTIAATQHVSLDSAQWLLTLTLLTGAASTPLIGRLGDGPHRRTVLLAGLGAVAVGSVLSATANGFAQLLVGRGLMGVG
MGLMPLALALARDLLPPHKMAPGVAALSITVATGAGLGYPLSGLLADTFDYHAGFWVAAALAAAGMAAVLVAVPGRASAR
TSHGRVDLRGAVLFAAALSPVLLALSEGESWGWLSPAVIALLVGGIGCGVAWALVELRTDNPLIELKYLAARPVLIADMC
AALAGFGMFNAMTLINRLAQAPTSTGYGFGASPAVLGLVILPLSAGTVLASRWSRWLGPRTGGGRGLLLCGLMAVALALF
GLAVSHDHLVELGAATFLFGVGIGLAFAAMPALIMGAVPPHETGSATSFNQVLRTAGGSVGSALGAALLAAHTPAGSVEP
TNSGYTVAFVVAGGVCALAALAALALPGTSSAATRPLGAARRTELETLEEESAGSTAAGLVMAEDVRS

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=425, Percent_Identity=25.6470588235294, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788710, Length=411, Percent_Identity=22.6277372262774, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790195, Length=439, Percent_Identity=24.8291571753986, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54534; Mature: 54534

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAERDIIQPRTSADEAADEAADEAAGEAADEVAEPKPPGPTAVARPSGGGGPHPRPGLL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCHHHH
LGVLVYCGLVIAVIGTLGTPLIPTIAATQHVSLDSAQWLLTLTLLTGAASTPLIGRLGDG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCC
PHRRTVLLAGLGAVAVGSVLSATANGFAQLLVGRGLMGVGMGLMPLALALARDLLPPHKM
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHCCCCHHC
APGVAALSITVATGAGLGYPLSGLLADTFDYHAGFWVAAALAAAGMAAVLVAVPGRASAR
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHCCCCCCCCC
TSHGRVDLRGAVLFAAALSPVLLALSEGESWGWLSPAVIALLVGGIGCGVAWALVELRTD
CCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
NPLIELKYLAARPVLIADMCAALAGFGMFNAMTLINRLAQAPTSTGYGFGASPAVLGLVI
CCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
LPLSAGTVLASRWSRWLGPRTGGGRGLLLCGLMAVALALFGLAVSHDHLVELGAATFLFG
HHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VGIGLAFAAMPALIMGAVPPHETGSATSFNQVLRTAGGSVGSALGAALLAAHTPAGSVEP
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
TNSGYTVAFVVAGGVCALAALAALALPGTSSAATRPLGAARRTELETLEEESAGSTAAGL
CCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
VMAEDVRS
EEHHHHCC
>Mature Secondary Structure
MRAERDIIQPRTSADEAADEAADEAAGEAADEVAEPKPPGPTAVARPSGGGGPHPRPGLL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCHHHH
LGVLVYCGLVIAVIGTLGTPLIPTIAATQHVSLDSAQWLLTLTLLTGAASTPLIGRLGDG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCC
PHRRTVLLAGLGAVAVGSVLSATANGFAQLLVGRGLMGVGMGLMPLALALARDLLPPHKM
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHCCCCHHC
APGVAALSITVATGAGLGYPLSGLLADTFDYHAGFWVAAALAAAGMAAVLVAVPGRASAR
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHCCCCCCCCC
TSHGRVDLRGAVLFAAALSPVLLALSEGESWGWLSPAVIALLVGGIGCGVAWALVELRTD
CCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
NPLIELKYLAARPVLIADMCAALAGFGMFNAMTLINRLAQAPTSTGYGFGASPAVLGLVI
CCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
LPLSAGTVLASRWSRWLGPRTGGGRGLLLCGLMAVALALFGLAVSHDHLVELGAATFLFG
HHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VGIGLAFAAMPALIMGAVPPHETGSATSFNQVLRTAGGSVGSALGAALLAAHTPAGSVEP
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
TNSGYTVAFVVAGGVCALAALAALALPGTSSAATRPLGAARRTELETLEEESAGSTAAGL
CCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
VMAEDVRS
EEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]