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The map label for this gene is purR [H]

Identifier: 158318659

GI number: 158318659

Start: 8437899

End: 8438936

Strand: Reverse

Name: purR [H]

Synonym: Franean1_6927

Alternate gene names: 158318659

Gene position: 8438936-8437899 (Counterclockwise)

Preceding gene: 158318660

Following gene: 158318658

Centisome position: 93.95

GC content: 74.28

Gene sequence:

>1038_bases
ATGGCCGGGACGGACGTTCCGAGCGTGAAGGACGTCGCGGCGCACGCCGGGGTGTCGCTGGGCACGGTGTCGAACGTGCT
CAACCGGCCGGAGCTGGTCGCCCGGCCCACCCGGGAACGGGTGCTCGCCTCGATCGCCACTCTCGGCTTCGTCCGCAACG
AGTCTGCGCGGCAACTGCGGGCCGGCGGCAGCCGGCTGCTCGCCTACGTGGTGCCCGACACCGCCAATCCCGTCTTCGCC
GACGTCGGCGCCGGTGTCCAGGAGGTCGCGGATCGCGCCGAGCTGGCGGTGTTCCTGTGTAACAGCGGCGAGGAGTCCCG
CCGGCAGGCGACCTACCTGAGCCTGCTCGAACAGCAGCGCGTCGAGGGAGTCCTTCTCACTCCGGTGGGCGAGGTCGACG
AGCCCCTGGTGTCGCTGGCGCGGCGGGGGACACCGGTCGTGCTCGTCGACCGCGCCGGCGGGCCGGGGATCTGTTCGGTG
ACGGTCGATGACGTGGCCGGCGGTGAGCTCGCTGTGTCCCATCTGCTCGACAGTGGCCACACCCGGATCGCCTTCGTCGG
CGGCCCGGGCCAGTGGCGGGTCGCCGACCGGCTCGCCGGCGCCCGCCGCGGCCTGCTGCACGCCGGTGCCGACCCGGACA
GCCTGCTGCGGGTCGACACCCGCGGCGCCGACGTCGCCGCCGGCCGCCGGGCCGGGGAACGACTGGCCGATCTGTCCGGC
AGCGGACGTCCCACGGCTGTGCTCTGCGCCAACGACATGGTCGCCCTCGGCCTGTTGCAGCAGGTGACGCGGCTCGGCCT
GCGCGTCCCCGACGATCTCGCCGTGGCCGGCTACGAGGACATCGAGCTCGCCGCCGTGGCGGCGGTTCCGCTGACCTCCG
TGGCGCGGCCGCGCCAGCTGCTGGGACGTACCGCCGCCGAGCTGCTGCTGGCGGAGACGCACGACCGCGCGGGACATGAG
CACAGCCAGGTCGTGCTGGCACCCGAGCTGGTGGTCCGGGCGTCCACCGGGCGCCGGCCGGCGGACATCGTCTCCTGA

Upstream 100 bases:

>100_bases
TGATCTCCGTGCCGGGTTCGACCACGAGGGCGTCCAGCACCCGTGGGGGAGATCCGGACGACAATCGGGGGAAGGGCCGG
GGACGACCGGAGGGGGCGCG

Downstream 100 bases:

>100_bases
GCACCGCCAAGGGCCCACGCGCCGGCGGGTGGCGTCCGGCGGCCGGGAGCAGTGGCATCGGCGTGCCACCGGCGTGCGCG
GCGGCACGGTCCGCGCTGGC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MAGTDVPSVKDVAAHAGVSLGTVSNVLNRPELVARPTRERVLASIATLGFVRNESARQLRAGGSRLLAYVVPDTANPVFA
DVGAGVQEVADRAELAVFLCNSGEESRRQATYLSLLEQQRVEGVLLTPVGEVDEPLVSLARRGTPVVLVDRAGGPGICSV
TVDDVAGGELAVSHLLDSGHTRIAFVGGPGQWRVADRLAGARRGLLHAGADPDSLLRVDTRGADVAAGRRAGERLADLSG
SGRPTAVLCANDMVALGLLQQVTRLGLRVPDDLAVAGYEDIELAAVAAVPLTSVARPRQLLGRTAAELLLAETHDRAGHE
HSQVVLAPELVVRASTGRRPADIVS

Sequences:

>Translated_345_residues
MAGTDVPSVKDVAAHAGVSLGTVSNVLNRPELVARPTRERVLASIATLGFVRNESARQLRAGGSRLLAYVVPDTANPVFA
DVGAGVQEVADRAELAVFLCNSGEESRRQATYLSLLEQQRVEGVLLTPVGEVDEPLVSLARRGTPVVLVDRAGGPGICSV
TVDDVAGGELAVSHLLDSGHTRIAFVGGPGQWRVADRLAGARRGLLHAGADPDSLLRVDTRGADVAAGRRAGERLADLSG
SGRPTAVLCANDMVALGLLQQVTRLGLRVPDDLAVAGYEDIELAAVAAVPLTSVARPRQLLGRTAAELLLAETHDRAGHE
HSQVVLAPELVVRASTGRRPADIVS
>Mature_344_residues
AGTDVPSVKDVAAHAGVSLGTVSNVLNRPELVARPTRERVLASIATLGFVRNESARQLRAGGSRLLAYVVPDTANPVFAD
VGAGVQEVADRAELAVFLCNSGEESRRQATYLSLLEQQRVEGVLLTPVGEVDEPLVSLARRGTPVVLVDRAGGPGICSVT
VDDVAGGELAVSHLLDSGHTRIAFVGGPGQWRVADRLAGARRGLLHAGADPDSLLRVDTRGADVAAGRRAGERLADLSGS
GRPTAVLCANDMVALGLLQQVTRLGLRVPDDLAVAGYEDIELAAVAAVPLTSVARPRQLLGRTAAELLLAETHDRAGHEH
SQVVLAPELVVRASTGRRPADIVS

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=333, Percent_Identity=30.9309309309309, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1790194, Length=332, Percent_Identity=32.8313253012048, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1787948, Length=340, Percent_Identity=31.1764705882353, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786540, Length=315, Percent_Identity=32.6984126984127, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI48994940, Length=308, Percent_Identity=28.8961038961039, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787906, Length=351, Percent_Identity=29.0598290598291, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788474, Length=340, Percent_Identity=29.7058823529412, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1789202, Length=295, Percent_Identity=29.1525423728814, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790715, Length=326, Percent_Identity=25.1533742331288, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787580, Length=313, Percent_Identity=27.7955271565495, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789068, Length=309, Percent_Identity=27.5080906148867, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789456, Length=321, Percent_Identity=26.4797507788162, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790689, Length=348, Percent_Identity=26.4367816091954, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35957; Mature: 35826

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTDVPSVKDVAAHAGVSLGTVSNVLNRPELVARPTRERVLASIATLGFVRNESARQLR
CCCCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHCCCHHHHHH
AGGSRLLAYVVPDTANPVFADVGAGVQEVADRAELAVFLCNSGEESRRQATYLSLLEQQR
CCCCEEEEEEECCCCCCHHHHHCCCHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHH
VEGVLLTPVGEVDEPLVSLARRGTPVVLVDRAGGPGICSVTVDDVAGGELAVSHLLDSGH
HCEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCC
TRIAFVGGPGQWRVADRLAGARRGLLHAGADPDSLLRVDTRGADVAAGRRAGERLADLSG
EEEEEEECCCCCHHHHHHHHHHHCCEECCCCHHHEEEEECCCCCHHHHHHHHHHHHHCCC
SGRPTAVLCANDMVALGLLQQVTRLGLRVPDDLAVAGYEDIELAAVAAVPLTSVARPRQL
CCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHEEHHHHCCHHHHCCHHHH
LGRTAAELLLAETHDRAGHEHSQVVLAPELVVRASTGRRPADIVS
HHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCCCCCHHCCC
>Mature Secondary Structure 
AGTDVPSVKDVAAHAGVSLGTVSNVLNRPELVARPTRERVLASIATLGFVRNESARQLR
CCCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHCCCHHHHHH
AGGSRLLAYVVPDTANPVFADVGAGVQEVADRAELAVFLCNSGEESRRQATYLSLLEQQR
CCCCEEEEEEECCCCCCHHHHHCCCHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHH
VEGVLLTPVGEVDEPLVSLARRGTPVVLVDRAGGPGICSVTVDDVAGGELAVSHLLDSGH
HCEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCC
TRIAFVGGPGQWRVADRLAGARRGLLHAGADPDSLLRVDTRGADVAAGRRAGERLADLSG
EEEEEEECCCCCHHHHHHHHHHHCCEECCCCHHHEEEEECCCCCHHHHHHHHHHHHHCCC
SGRPTAVLCANDMVALGLLQQVTRLGLRVPDDLAVAGYEDIELAAVAAVPLTSVARPRQL
CCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHEEHHHHCCHHHHCCHHHH
LGRTAAELLLAETHDRAGHEHSQVVLAPELVVRASTGRRPADIVS
HHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA