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The map label for this gene is gadA [H]

Identifier: 158318636

GI number: 158318636

Start: 8411592

End: 8413013

Strand: Direct

Name: gadA [H]

Synonym: Franean1_6904

Alternate gene names: 158318636

Gene position: 8411592-8413013 (Clockwise)

Preceding gene: 158318635

Following gene: 158318637

Centisome position: 93.65

GC content: 70.68

Gene sequence:

>1422_bases
ATGGCCCTACACGGCCGGGCGAGAGGCAGGCGCGGCGACCAGGCCGTGGAGGTACGGCCGCACCTGGTCATCCCGGACGA
GGACGGGCCCGTCCCCCGGTACCGCATGCCGCGCTCCTCGATGTCCGCCGAGACCGCCTACCAGATCGTGCGCGACGAGC
TGATGCTGGACGGCAACGCCCGACTGAACCTGGCCACGTTCGTGACCACCTGGATGGACGAGCACGCCGACCGGCTGATG
ACCGAGTGTGCGGCGAAGAACATGATCGACAAGGACGAGTACCCGCAGACGGCCGAGCTCGAGGCGCGGTGCGTCAACAT
GCTCGCCGACCTGTGGCACGCCCCCGACGCCACCGACGCCGTCGGCTGCTCGACGACCGGGTCCTCCGAGGCGTGCATGC
TCGCCGGGCTGGCGATGCTACGCCGGTGGCGCTCGACCAGGGAGCCCCACCGCGGGGAACAGGGCGGCGGGCAACGGGGC
ACCGGTGCGCGGCCGAACATCGTCATGGGTGCCAACGTGCAGGTGTGCTGGGAGAAGTTCGCCCGCTACTGGGACGTCGA
GCCCCGGCTGATGCCACTCGCGCCCGGGCGGACCCACCTCACCGCCCCGGAGGCCGTCGCCCGCTGCGACGAGAACACGA
TCGGGGTCGTGGCCGTCCTCGGCTCGACGTTCGACGGGACGTACGAGCCGGTGGCCGAGATCGTCGCCGCGTTGGACCAG
CTGGCGGCCTCGGGCGGCCCGGACGTCCCGGTGCACGTCGACGGGGCTTCCGGCGGGTTCATCGCCCCGTTCTGCGACCC
CGACCTGGTCTGGGATTTCCGGCTCGAGCGCGTGGTCTCCATCAACGCCTCCGGGCACAAGTACGGGCTGGTCTATCCCG
GGGTCGGCTGGGCGCTCTGGCGCGACGCCCGGCACCTTCCGGCCGAGCTCGTCTTCGACGTGGACTATCTCGGCGGCTCG
ATGCCGACCTTCGCGTTGAACTTCTCCCGGCCGGGAGCACAGGTCGTCGCGCAGTACTACTCTCTGCTACGACTCGGCCG
GGCCGGTTACCGGCATACCGCCCGCACGTGCCGTGACAATGCGCGTTGGCTGGCGGATGAAATCGCGAAGCTCGGCCCGT
TCGAGCTGATCTCGGACGGTTCGGGGATCCCGGCCTTCGCGTTCACGACGAGGGATGCGGCCGAGTTCAGCGTCTTCGAG
GTCTCCGAGGCGCTGCGCGCCCGTGGCTGGCTGGTGCCCGCATACCGATTCCCGCCTGATCTGGCCGAGCTGGCGGTACT
GCGGATCGTCGTGCGGGCCGAGTTCAGCCGGGATCTGGCGCATCTGCTGGTCGAGGACCTGCACCGGGTGGTCGGACGAC
TCTCCGGCCCCCGGTGGCGGACCGCGGCCGGCGGCGCCGATCTCGCGTCGTTCCACCATTGA

Upstream 100 bases:

>100_bases
AACTTCGATTACGTATCGCGCCCGGAAAGTCACCTGGCGTGATTCCCGATCTCGTCGCAGACTCTGGTCGACGACGGATG
CGGCCGAGGGAGGGACGTCC

Downstream 100 bases:

>100_bases
TCCACCCACCGTCCGAGCCGCTCCGAAACCGACACGACCCGACGCACGGAAAACATTAGGACACACACAGGCCAACACAG
GCCGCGGGGCCGGAGATGCG

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-alpha [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MALHGRARGRRGDQAVEVRPHLVIPDEDGPVPRYRMPRSSMSAETAYQIVRDELMLDGNARLNLATFVTTWMDEHADRLM
TECAAKNMIDKDEYPQTAELEARCVNMLADLWHAPDATDAVGCSTTGSSEACMLAGLAMLRRWRSTREPHRGEQGGGQRG
TGARPNIVMGANVQVCWEKFARYWDVEPRLMPLAPGRTHLTAPEAVARCDENTIGVVAVLGSTFDGTYEPVAEIVAALDQ
LAASGGPDVPVHVDGASGGFIAPFCDPDLVWDFRLERVVSINASGHKYGLVYPGVGWALWRDARHLPAELVFDVDYLGGS
MPTFALNFSRPGAQVVAQYYSLLRLGRAGYRHTARTCRDNARWLADEIAKLGPFELISDGSGIPAFAFTTRDAAEFSVFE
VSEALRARGWLVPAYRFPPDLAELAVLRIVVRAEFSRDLAHLLVEDLHRVVGRLSGPRWRTAAGGADLASFHH

Sequences:

>Translated_473_residues
MALHGRARGRRGDQAVEVRPHLVIPDEDGPVPRYRMPRSSMSAETAYQIVRDELMLDGNARLNLATFVTTWMDEHADRLM
TECAAKNMIDKDEYPQTAELEARCVNMLADLWHAPDATDAVGCSTTGSSEACMLAGLAMLRRWRSTREPHRGEQGGGQRG
TGARPNIVMGANVQVCWEKFARYWDVEPRLMPLAPGRTHLTAPEAVARCDENTIGVVAVLGSTFDGTYEPVAEIVAALDQ
LAASGGPDVPVHVDGASGGFIAPFCDPDLVWDFRLERVVSINASGHKYGLVYPGVGWALWRDARHLPAELVFDVDYLGGS
MPTFALNFSRPGAQVVAQYYSLLRLGRAGYRHTARTCRDNARWLADEIAKLGPFELISDGSGIPAFAFTTRDAAEFSVFE
VSEALRARGWLVPAYRFPPDLAELAVLRIVVRAEFSRDLAHLLVEDLHRVVGRLSGPRWRTAAGGADLASFHH
>Mature_472_residues
ALHGRARGRRGDQAVEVRPHLVIPDEDGPVPRYRMPRSSMSAETAYQIVRDELMLDGNARLNLATFVTTWMDEHADRLMT
ECAAKNMIDKDEYPQTAELEARCVNMLADLWHAPDATDAVGCSTTGSSEACMLAGLAMLRRWRSTREPHRGEQGGGQRGT
GARPNIVMGANVQVCWEKFARYWDVEPRLMPLAPGRTHLTAPEAVARCDENTIGVVAVLGSTFDGTYEPVAEIVAALDQL
AASGGPDVPVHVDGASGGFIAPFCDPDLVWDFRLERVVSINASGHKYGLVYPGVGWALWRDARHLPAELVFDVDYLGGSM
PTFALNFSRPGAQVVAQYYSLLRLGRAGYRHTARTCRDNARWLADEIAKLGPFELISDGSGIPAFAFTTRDAAEFSVFEV
SEALRARGWLVPAYRFPPDLAELAVLRIVVRAEFSRDLAHLLVEDLHRVVGRLSGPRWRTAAGGADLASFHH

Specific function: Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit th

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=390, Percent_Identity=24.6153846153846, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1787769, Length=447, Percent_Identity=50.7829977628635, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1789934, Length=447, Percent_Identity=50.7829977628635, Blast_Score=431, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17557272, Length=326, Percent_Identity=26.3803680981595, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17543922, Length=411, Percent_Identity=24.5742092457421, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25148342, Length=325, Percent_Identity=26.4615384615385, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323905, Length=475, Percent_Identity=40.8421052631579, Blast_Score=345, Evalue=1e-95,
Organism=Saccharomyces cerevisiae, GI6320500, Length=372, Percent_Identity=23.3870967741935, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21355963, Length=379, Percent_Identity=25.3298153034301, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24654344, Length=379, Percent_Identity=25.3298153034301, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR021115 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 51976; Mature: 51845

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALHGRARGRRGDQAVEVRPHLVIPDEDGPVPRYRMPRSSMSAETAYQIVRDELMLDGNA
CCCCCCCCCCCCCCEEEECCEEEECCCCCCCCCCCCCCHHCCHHHHHHHHHHHHEECCCC
RLNLATFVTTWMDEHADRLMTECAAKNMIDKDEYPQTAELEARCVNMLADLWHAPDATDA
CEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
VGCSTTGSSEACMLAGLAMLRRWRSTREPHRGEQGGGQRGTGARPNIVMGANVQVCWEKF
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHH
ARYWDVEPRLMPLAPGRTHLTAPEAVARCDENTIGVVAVLGSTFDGTYEPVAEIVAALDQ
HHHCCCCCCEEECCCCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
LAASGGPDVPVHVDGASGGFIAPFCDPDLVWDFRLERVVSINASGHKYGLVYPGVGWALW
HHHCCCCCCEEEEECCCCCEECCCCCCCCEEHEEEEEEEEECCCCCEEEEEECCCCHHHH
RDARHLPAELVFDVDYLGGSMPTFALNFSRPGAQVVAQYYSLLRLGRAGYRHTARTCRDN
HHHHCCCHHHEEEHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ARWLADEIAKLGPFELISDGSGIPAFAFTTRDAAEFSVFEVSEALRARGWLVPAYRFPPD
HHHHHHHHHHCCCHHEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCCH
LAELAVLRIVVRAEFSRDLAHLLVEDLHRVVGRLSGPRWRTAAGGADLASFHH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHCCC
>Mature Secondary Structure 
ALHGRARGRRGDQAVEVRPHLVIPDEDGPVPRYRMPRSSMSAETAYQIVRDELMLDGNA
CCCCCCCCCCCCCEEEECCEEEECCCCCCCCCCCCCCHHCCHHHHHHHHHHHHEECCCC
RLNLATFVTTWMDEHADRLMTECAAKNMIDKDEYPQTAELEARCVNMLADLWHAPDATDA
CEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
VGCSTTGSSEACMLAGLAMLRRWRSTREPHRGEQGGGQRGTGARPNIVMGANVQVCWEKF
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHH
ARYWDVEPRLMPLAPGRTHLTAPEAVARCDENTIGVVAVLGSTFDGTYEPVAEIVAALDQ
HHHCCCCCCEEECCCCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
LAASGGPDVPVHVDGASGGFIAPFCDPDLVWDFRLERVVSINASGHKYGLVYPGVGWALW
HHHCCCCCCEEEEECCCCCEECCCCCCCCEEHEEEEEEEEECCCCCEEEEEECCCCHHHH
RDARHLPAELVFDVDYLGGSMPTFALNFSRPGAQVVAQYYSLLRLGRAGYRHTARTCRDN
HHHHCCCHHHEEEHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ARWLADEIAKLGPFELISDGSGIPAFAFTTRDAAEFSVFEVSEALRARGWLVPAYRFPPD
HHHHHHHHHHCCCHHEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCCH
LAELAVLRIVVRAEFSRDLAHLLVEDLHRVVGRLSGPRWRTAAGGADLASFHH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]