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The map label for this gene is nifB [H]

Identifier: 158318603

GI number: 158318603

Start: 8375928

End: 8377895

Strand: Reverse

Name: nifB [H]

Synonym: Franean1_6871

Alternate gene names: 158318603

Gene position: 8377895-8375928 (Counterclockwise)

Preceding gene: 158318604

Following gene: 158318602

Centisome position: 93.27

GC content: 71.24

Gene sequence:

>1968_bases
GTGAACAACGAGCCCGTCATGCTTCCGGTGCTGCCCGCCGCCACGGCGGAGCTGCTGACCGGTCGGACGGCCACACCTGG
TCAGAACGGCTCGCCCGCCCAGGGCGCGGCGCCCGCCCCGGGTGGCGGCTGCGCGTCCAAGAAGAGCTGCGGCAGCAGCA
CTCCGGCCACCGATCCGGAGATCGCCGAGAAGATCGCCAACCACCCCTGCTACAGCTCCGAGGCGCACCAGTACTACGCC
CGGATGCACGTGGCGGTGGCGCCCGGCTGCAACATCCAGTGCAACTACTGCAACCGCAAGTACGACTGTGCGAACGAGAG
CCGGCCCGGCGTCACCAGCGACCTGCTCACCCCCGAGGACGCGCTCGCCAAGGTCAAGCTGGTCGCCAGCGAGATCAAGC
AGATGAGCGTGCTGGGCATCGCCGGCCCCGGCGACCCGCTCGCCAACCCGAAGCGCACCTTCCGCACCCTGGAACTGGTC
GCCCGGGACTGCCCCGACATCAAGCTGTGCCTGTCGACGAACGGGCTCACCCTGCCCGACCACGTCGACCGCATCGCCGC
GCTGAACGTCGACCACGTCACGATCACGATCAACATGATCGACCCCGAGGTCGGCGAGCGGATCTACCCCTGGGTCGCTT
ACAAGGGCAAGAAGTACACGGGCCGGGAGGCGTCGAAGATCCTCTCCGAGCGCCAGCTCGAGGGCCTCGCCATGCTGGCC
GAGCGCAAGATCCTCGCCAAGGTCAACTCGGTGATGATCCCGGGGGTCAACGACGAGCACCTCGTCGAGGTCTCGAAGAC
CGTCCGCGGGCTCGGTGCCTTCCTGCACAACGTGATGCCGCTGGTCTCCGCCCCGGAGCACGGCACCGTGTTCGGCCTCG
CCGGCCAGCCGGGCCCGACGCCCCAGCAGCTCAAGGCTCTCCAGGACCGCTGCGAGGGCGAAGACGGCGCCGACATGAAC
ATGATGCGGCACTGCCGGCAGTGCCGTGCGGACGCCGTCGGCCTGCTCGGCGAGGACCGCGGTGACGAGTTCACCCCGGA
TACCTACCAGGGCCGCGAGATCGCCTACGACCTGGAAGGCCGCCTTGCCGCCCACGCCGAGATCGAGCGGTGGCGCCAGG
AGACCCGCGCCGAGCGCGAGACGGTGACCGTCGCCGGACCGGCGACCAAGCCCGACGACGTCGTGCTGGTCGCCGTCGCG
ACCAAGGGCAGCGGCGTGGTCAACCAGCACTTCGGCCACGCCTCCGAGTTCTGGATCTACGAGGCCGGCCGCAGCTGGGC
CCGGCTCGTCCAGACCCGCGACGTGCAGCGCTACTGCGAGGGCCCGACCGAGTGCGGGGACGGCGCCTCGGTGCTCGACA
AGACCATCGAGATGCTCTCCGACTGCTCCGCCGTCCTGTGCAGCAAGATCGGCCCGGGGCCGTACGACGCGCTCGAGGCC
GCCGGGATCGAGCCGGTCGAGGTCTACGAGCTGATCGAGAAGGCCGTCGCCACCGTCGGCGGCCGGCTCACCGCGGCCGC
CCGCGCGGCCGTCCCGGTGAACCCCGACCCCGCCGGCACCGGTTTCGCGGACCTCGACGAGATCGACCTCGACGAGGTGG
AGGCCGAGATGGCCGAGGCCGAGCTGGTCCAGGCCGAACTGGTGGACGTCGACCTGCTCGACCCGGACCTCATGGACGAC
GACCCGTTCGAGTTCGCCGTCACCGTCCGCCCCGGCTCCTGCTCGGGTGGGGGTGGCAAGTCCGGCTCCTGCTCCGGCGG
CGGCCACCGCCACGCCGCCGGTTCCGGCCACCGCCACGACGTCGAGCACGCCCGTACCGACCCGCGCACCGAGAAGTCCC
GCGCGGAGCGGACCACCCCGGAGAAGGCGCGCGCCGACGGTGCCCGCCCCGCCCACACCCGTTCCGTCGGCACCGGCTCC
CACAGCGCCGGCACCGACGTCACAGACCTGGAGGTAGCGCGGTCATGA

Upstream 100 bases:

>100_bases
CCAGCCGGGCCGCCGACGCCACCGCCCATGCCACAAGTTCCGCGGCTGACAGCAGCGCCGCCGTGACCAGCGGGACCACC
GACAAGGAGGAGGGGCTGTC

Downstream 100 bases:

>100_bases
CCCTCGTCCAGAGCACCATCGAACTGACCGAGCGCGCCCGCGCCCAGGTGCGGACCCTGCTCAGCGGCAGCGTGGAGACG
GCCGGCTTCGGCCTGCGCGT

Product: nitrogenase cofactor biosynthesis protein NifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MNNEPVMLPVLPAATAELLTGRTATPGQNGSPAQGAAPAPGGGCASKKSCGSSTPATDPEIAEKIANHPCYSSEAHQYYA
RMHVAVAPGCNIQCNYCNRKYDCANESRPGVTSDLLTPEDALAKVKLVASEIKQMSVLGIAGPGDPLANPKRTFRTLELV
ARDCPDIKLCLSTNGLTLPDHVDRIAALNVDHVTITINMIDPEVGERIYPWVAYKGKKYTGREASKILSERQLEGLAMLA
ERKILAKVNSVMIPGVNDEHLVEVSKTVRGLGAFLHNVMPLVSAPEHGTVFGLAGQPGPTPQQLKALQDRCEGEDGADMN
MMRHCRQCRADAVGLLGEDRGDEFTPDTYQGREIAYDLEGRLAAHAEIERWRQETRAERETVTVAGPATKPDDVVLVAVA
TKGSGVVNQHFGHASEFWIYEAGRSWARLVQTRDVQRYCEGPTECGDGASVLDKTIEMLSDCSAVLCSKIGPGPYDALEA
AGIEPVEVYELIEKAVATVGGRLTAAARAAVPVNPDPAGTGFADLDEIDLDEVEAEMAEAELVQAELVDVDLLDPDLMDD
DPFEFAVTVRPGSCSGGGGKSGSCSGGGHRHAAGSGHRHDVEHARTDPRTEKSRAERTTPEKARADGARPAHTRSVGTGS
HSAGTDVTDLEVARS

Sequences:

>Translated_655_residues
MNNEPVMLPVLPAATAELLTGRTATPGQNGSPAQGAAPAPGGGCASKKSCGSSTPATDPEIAEKIANHPCYSSEAHQYYA
RMHVAVAPGCNIQCNYCNRKYDCANESRPGVTSDLLTPEDALAKVKLVASEIKQMSVLGIAGPGDPLANPKRTFRTLELV
ARDCPDIKLCLSTNGLTLPDHVDRIAALNVDHVTITINMIDPEVGERIYPWVAYKGKKYTGREASKILSERQLEGLAMLA
ERKILAKVNSVMIPGVNDEHLVEVSKTVRGLGAFLHNVMPLVSAPEHGTVFGLAGQPGPTPQQLKALQDRCEGEDGADMN
MMRHCRQCRADAVGLLGEDRGDEFTPDTYQGREIAYDLEGRLAAHAEIERWRQETRAERETVTVAGPATKPDDVVLVAVA
TKGSGVVNQHFGHASEFWIYEAGRSWARLVQTRDVQRYCEGPTECGDGASVLDKTIEMLSDCSAVLCSKIGPGPYDALEA
AGIEPVEVYELIEKAVATVGGRLTAAARAAVPVNPDPAGTGFADLDEIDLDEVEAEMAEAELVQAELVDVDLLDPDLMDD
DPFEFAVTVRPGSCSGGGGKSGSCSGGGHRHAAGSGHRHDVEHARTDPRTEKSRAERTTPEKARADGARPAHTRSVGTGS
HSAGTDVTDLEVARS
>Mature_655_residues
MNNEPVMLPVLPAATAELLTGRTATPGQNGSPAQGAAPAPGGGCASKKSCGSSTPATDPEIAEKIANHPCYSSEAHQYYA
RMHVAVAPGCNIQCNYCNRKYDCANESRPGVTSDLLTPEDALAKVKLVASEIKQMSVLGIAGPGDPLANPKRTFRTLELV
ARDCPDIKLCLSTNGLTLPDHVDRIAALNVDHVTITINMIDPEVGERIYPWVAYKGKKYTGREASKILSERQLEGLAMLA
ERKILAKVNSVMIPGVNDEHLVEVSKTVRGLGAFLHNVMPLVSAPEHGTVFGLAGQPGPTPQQLKALQDRCEGEDGADMN
MMRHCRQCRADAVGLLGEDRGDEFTPDTYQGREIAYDLEGRLAAHAEIERWRQETRAERETVTVAGPATKPDDVVLVAVA
TKGSGVVNQHFGHASEFWIYEAGRSWARLVQTRDVQRYCEGPTECGDGASVLDKTIEMLSDCSAVLCSKIGPGPYDALEA
AGIEPVEVYELIEKAVATVGGRLTAAARAAVPVNPDPAGTGFADLDEIDLDEVEAEMAEAELVQAELVDVDLLDPDLMDD
DPFEFAVTVRPGSCSGGGGKSGSCSGGGHRHAAGSGHRHDVEHARTDPRTEKSRAERTTPEKARADGARPAHTRSVGTGS
HSAGTDVTDLEVARS

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 69731; Mature: 69731

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNEPVMLPVLPAATAELLTGRTATPGQNGSPAQGAAPAPGGGCASKKSCGSSTPATDPE
CCCCCEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
IAEKIANHPCYSSEAHQYYARMHVAVAPGCNIQCNYCNRKYDCANESRPGVTSDLLTPED
HHHHHHCCCCCCCHHHHHHHHEEEEECCCCCEEECCCCCCCCCCCCCCCCCCHHCCCCHH
ALAKVKLVASEIKQMSVLGIAGPGDPLANPKRTFRTLELVARDCPDIKLCLSTNGLTLPD
HHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCH
HVDRIAALNVDHVTITINMIDPEVGERIYPWVAYKGKKYTGREASKILSERQLEGLAMLA
HHHHHHHCCCCEEEEEEEEECHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
ERKILAKVNSVMIPGVNDEHLVEVSKTVRGLGAFLHNVMPLVSAPEHGTVFGLAGQPGPT
HHHHHHHHHCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCC
PQQLKALQDRCEGEDGADMNMMRHCRQCRADAVGLLGEDRGDEFTPDTYQGREIAYDLEG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCC
RLAAHAEIERWRQETRAERETVTVAGPATKPDDVVLVAVATKGSGVVNQHFGHASEFWIY
CHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCEEEEEEECCCCCCHHHHCCCCCCEEEE
EAGRSWARLVQTRDVQRYCEGPTECGDGASVLDKTIEMLSDCSAVLCSKIGPGPYDALEA
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
AGIEPVEVYELIEKAVATVGGRLTAAARAAVPVNPDPAGTGFADLDEIDLDEVEAEMAEA
CCCCHHHHHHHHHHHHHHHCCHHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ELVQAELVDVDLLDPDLMDDDPFEFAVTVRPGSCSGGGGKSGSCSGGGHRHAAGSGHRHD
HHHHHHHHEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VEHARTDPRTEKSRAERTTPEKARADGARPAHTRSVGTGSHSAGTDVTDLEVARS
HHHHCCCCCHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCC
>Mature Secondary Structure
MNNEPVMLPVLPAATAELLTGRTATPGQNGSPAQGAAPAPGGGCASKKSCGSSTPATDPE
CCCCCEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
IAEKIANHPCYSSEAHQYYARMHVAVAPGCNIQCNYCNRKYDCANESRPGVTSDLLTPED
HHHHHHCCCCCCCHHHHHHHHEEEEECCCCCEEECCCCCCCCCCCCCCCCCCHHCCCCHH
ALAKVKLVASEIKQMSVLGIAGPGDPLANPKRTFRTLELVARDCPDIKLCLSTNGLTLPD
HHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCH
HVDRIAALNVDHVTITINMIDPEVGERIYPWVAYKGKKYTGREASKILSERQLEGLAMLA
HHHHHHHCCCCEEEEEEEEECHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
ERKILAKVNSVMIPGVNDEHLVEVSKTVRGLGAFLHNVMPLVSAPEHGTVFGLAGQPGPT
HHHHHHHHHCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCC
PQQLKALQDRCEGEDGADMNMMRHCRQCRADAVGLLGEDRGDEFTPDTYQGREIAYDLEG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCC
RLAAHAEIERWRQETRAERETVTVAGPATKPDDVVLVAVATKGSGVVNQHFGHASEFWIY
CHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCEEEEEEECCCCCCHHHHCCCCCCEEEE
EAGRSWARLVQTRDVQRYCEGPTECGDGASVLDKTIEMLSDCSAVLCSKIGPGPYDALEA
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
AGIEPVEVYELIEKAVATVGGRLTAAARAAVPVNPDPAGTGFADLDEIDLDEVEAEMAEA
CCCCHHHHHHHHHHHHHHHCCHHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ELVQAELVDVDLLDPDLMDDDPFEFAVTVRPGSCSGGGGKSGSCSGGGHRHAAGSGHRHD
HHHHHHHHEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VEHARTDPRTEKSRAERTTPEKARADGARPAHTRSVGTGSHSAGTDVTDLEVARS
HHHHCCCCCHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7642138 [H]