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The map label for this gene is hetN [H]

Identifier: 158318574

GI number: 158318574

Start: 8342970

End: 8343785

Strand: Direct

Name: hetN [H]

Synonym: Franean1_6842

Alternate gene names: 158318574

Gene position: 8342970-8343785 (Clockwise)

Preceding gene: 158318572

Following gene: 158318575

Centisome position: 92.89

GC content: 70.34

Gene sequence:

>816_bases
GTGGGATCGTTGGATGGCCGCGTAGCAATCGTCACCGGCGCCAGTCGCGGTATCGGGTCGCGCATCGCCGCGCGCCTGGC
CGCGGAGGGGGCGGCGGTCGCCCTGGTCGCGCGCACGGTCGACGCCGGTACTTCCCGGCTGCCAGGCTCGATCACCGAAG
TGATCGAGAACATCAAGGCCGGCGGCGGTCACGCCGTCGCCATTCCCGCCGATCTCACCGCTCCCGACGACGTCGAGACG
ATCATCGAACGTGCGGAGGCAGCCGTCGGCCCGGCGGACATTCTGGTCAACAACGCCGGGGTCAATTTCTACGGACCTGC
GCTCGACATCCCGCCGCGCCGTTACGGGCTGATGTTCCAGATGATGGTCCACACCCCGTTCCGGCTCTGCCAGCTCGTGG
TTCCCGGGATGGTCAGCCGCGGGCAGGGCTGGATCGTGAACATCACGTCCAAGCAGGCCCGGCACCCGATCGGCCCGCCC
TACCCGGAGTGGGCCAGCGACGGGTGCGTCCCCTACGGCATGTGCAAGGCCGCGCTGGACCGCCTGACCACCGGCCTGGC
CGCGGAGCTCGAGGGCACCGGGGTGGCGGTGAACGCACTCGGGACGTCCGGGCTGGTCATGACGCCCGGTGTCGCCGCCG
TCTCGCCACACACTCCGGACAACGCCCCGGTGGAACCGGACGAGGCCATGGCCGACGCGGCCCTGCTGCTGTGCAGCGGG
AACCCCGCGGAGCTGACCGGGCGGATCACCTACAGCATGGAATACCTCGGCAGGCCGTTCCCGAACGGGCCATGGGCCCT
GTCCGCCACGTTCTGA

Upstream 100 bases:

>100_bases
CAGGCAGGTAAGTATGTGAGCACCCTGTTAGCATGGCGCCCTACGGATCCGTCCCATGGCGTGCCGGGCCCGGCGCGACG
ACGTCGATCGTGAGGTTTTC

Downstream 100 bases:

>100_bases
CCAGCGACCACAACCTGATCAGAGACCACCACTGATCAGAGACCACCACCACGCTTTCCGACCGGTCCGGCGGCAGGGCT
GCCGCCGGGCCACGAGTGAA

Product: short-chain dehydrogenase/reductase SDR

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MGSLDGRVAIVTGASRGIGSRIAARLAAEGAAVALVARTVDAGTSRLPGSITEVIENIKAGGGHAVAIPADLTAPDDVET
IIERAEAAVGPADILVNNAGVNFYGPALDIPPRRYGLMFQMMVHTPFRLCQLVVPGMVSRGQGWIVNITSKQARHPIGPP
YPEWASDGCVPYGMCKAALDRLTTGLAAELEGTGVAVNALGTSGLVMTPGVAAVSPHTPDNAPVEPDEAMADAALLLCSG
NPAELTGRITYSMEYLGRPFPNGPWALSATF

Sequences:

>Translated_271_residues
MGSLDGRVAIVTGASRGIGSRIAARLAAEGAAVALVARTVDAGTSRLPGSITEVIENIKAGGGHAVAIPADLTAPDDVET
IIERAEAAVGPADILVNNAGVNFYGPALDIPPRRYGLMFQMMVHTPFRLCQLVVPGMVSRGQGWIVNITSKQARHPIGPP
YPEWASDGCVPYGMCKAALDRLTTGLAAELEGTGVAVNALGTSGLVMTPGVAAVSPHTPDNAPVEPDEAMADAALLLCSG
NPAELTGRITYSMEYLGRPFPNGPWALSATF
>Mature_270_residues
GSLDGRVAIVTGASRGIGSRIAARLAAEGAAVALVARTVDAGTSRLPGSITEVIENIKAGGGHAVAIPADLTAPDDVETI
IERAEAAVGPADILVNNAGVNFYGPALDIPPRRYGLMFQMMVHTPFRLCQLVVPGMVSRGQGWIVNITSKQARHPIGPPY
PEWASDGCVPYGMCKAALDRLTTGLAAELEGTGVAVNALGTSGLVMTPGVAAVSPHTPDNAPVEPDEAMADAALLLCSGN
PAELTGRITYSMEYLGRPFPNGPWALSATF

Specific function: May be involved in repressing heterocyst differentiation and may be essential for preventing all vegetative cells from differentiating [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40254986, Length=199, Percent_Identity=31.1557788944724, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI32483357, Length=249, Percent_Identity=28.9156626506024, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI126723191, Length=198, Percent_Identity=30.8080808080808, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI7705925, Length=275, Percent_Identity=29.8181818181818, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI19923817, Length=200, Percent_Identity=26.5, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1787905, Length=255, Percent_Identity=33.3333333333333, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790717, Length=258, Percent_Identity=27.5193798449612, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082100, Length=234, Percent_Identity=29.9145299145299, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787335, Length=198, Percent_Identity=28.7878787878788, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI2367175, Length=229, Percent_Identity=29.2576419213974, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788459, Length=222, Percent_Identity=29.2792792792793, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789208, Length=149, Percent_Identity=31.5436241610738, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789378, Length=258, Percent_Identity=27.1317829457364, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71982365, Length=258, Percent_Identity=29.8449612403101, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17558628, Length=258, Percent_Identity=29.4573643410853, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562910, Length=222, Percent_Identity=32.8828828828829, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17561402, Length=199, Percent_Identity=32.6633165829146, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17538480, Length=275, Percent_Identity=28, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17562906, Length=206, Percent_Identity=31.5533980582524, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17560150, Length=204, Percent_Identity=33.8235294117647, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994604, Length=213, Percent_Identity=31.4553990610329, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17560332, Length=225, Percent_Identity=32.4444444444444, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17562908, Length=211, Percent_Identity=29.3838862559242, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=228, Percent_Identity=30.2631578947368, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=211, Percent_Identity=30.8056872037915, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17551412, Length=264, Percent_Identity=27.6515151515151, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17562990, Length=222, Percent_Identity=30.6306306306306, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17544670, Length=234, Percent_Identity=29.0598290598291, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17555612, Length=205, Percent_Identity=30.7317073170732, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=211, Percent_Identity=30.3317535545024, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17536025, Length=201, Percent_Identity=29.3532338308458, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=211, Percent_Identity=30.3317535545024, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17560676, Length=201, Percent_Identity=28.3582089552239, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=240, Percent_Identity=29.1666666666667, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17565030, Length=222, Percent_Identity=31.0810810810811, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17568967, Length=230, Percent_Identity=30.8695652173913, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538486, Length=206, Percent_Identity=29.6116504854369, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=211, Percent_Identity=31.2796208530806, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=253, Percent_Identity=30.8300395256917, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21357673, Length=259, Percent_Identity=31.2741312741313, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI23397609, Length=218, Percent_Identity=32.1100917431193, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357041, Length=209, Percent_Identity=32.0574162679426, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI28571526, Length=218, Percent_Identity=31.651376146789, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24644339, Length=212, Percent_Identity=27.3584905660377, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 27923; Mature: 27792

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSLDGRVAIVTGASRGIGSRIAARLAAEGAAVALVARTVDAGTSRLPGSITEVIENIKA
CCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEHHCCCCCCCCCHHHHHHHHHHC
GGGHAVAIPADLTAPDDVETIIERAEAAVGPADILVNNAGVNFYGPALDIPPRRYGLMFQ
CCCEEEEECCCCCCCCHHHHHHHHHHHCCCCHHEEEECCCCEEECCCCCCCCHHHHHHHH
MMVHTPFRLCQLVVPGMVSRGQGWIVNITSKQARHPIGPPYPEWASDGCVPYGMCKAALD
HHHHCHHHHHHHHHCCHHCCCCCEEEEECCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RLTTGLAAELEGTGVAVNALGTSGLVMTPGVAAVSPHTPDNAPVEPDEAMADAALLLCSG
HHHHHHHEEECCCCEEEEECCCCCEEECCCCEEECCCCCCCCCCCHHHHHHCEEEEEECC
NPAELTGRITYSMEYLGRPFPNGPWALSATF
CCCCEEEEEEEEHHHHCCCCCCCCEEEEECC
>Mature Secondary Structure 
GSLDGRVAIVTGASRGIGSRIAARLAAEGAAVALVARTVDAGTSRLPGSITEVIENIKA
CCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEHHCCCCCCCCCHHHHHHHHHHC
GGGHAVAIPADLTAPDDVETIIERAEAAVGPADILVNNAGVNFYGPALDIPPRRYGLMFQ
CCCEEEEECCCCCCCCHHHHHHHHHHHCCCCHHEEEECCCCEEECCCCCCCCHHHHHHHH
MMVHTPFRLCQLVVPGMVSRGQGWIVNITSKQARHPIGPPYPEWASDGCVPYGMCKAALD
HHHHCHHHHHHHHHCCHHCCCCCEEEEECCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RLTTGLAAELEGTGVAVNALGTSGLVMTPGVAAVSPHTPDNAPVEPDEAMADAALLLCSG
HHHHHHHEEECCCCEEEEECCCCCEEECCCCEEECCCCCCCCCCCHHHHHHCEEEEEECC
NPAELTGRITYSMEYLGRPFPNGPWALSATF
CCCCEEEEEEEEHHHHCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8157596; 11759840 [H]