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The map label for this gene is ssuD [C]

Identifier: 158318540

GI number: 158318540

Start: 8299585

End: 8300559

Strand: Reverse

Name: ssuD [C]

Synonym: Franean1_6808

Alternate gene names: 158318540

Gene position: 8300559-8299585 (Counterclockwise)

Preceding gene: 158318551

Following gene: 158318537

Centisome position: 92.41

GC content: 68.41

Gene sequence:

>975_bases
GTGTGTTTTGAATCCACCCCGGTGGATCTCCCGAGTCACCTGCTCCCGACAGTTCCTCTCGACGAGGTGAAAATGCGCTT
CATCTACCATTACCCCGAGACCAACGGCACCGACCGCGACATGCTCGACGCCGGCGCGCTGGCCGAGGTCGCGGCAGCAG
CCGAGCGGGCGGGATTCTACGGATTCTGCCTCAGCGAGCACCCGGCCCCGAGTGCCCGATGGCTGGCCTCCGGCGGGCAC
CAGAGCCTCGATCCGTTCGTCGCGCTCGGCCACGTCGCCGCCGTGACCCAGCGGTTGCGGCTGCACACGAACCTTTCCGT
CGCGCCCTACCGGAACCCGTTCCTGCTTGCCAAGGCGGCGGCGACCGTCGACAAGCTCTCCGGTGGACGGATCGTGCTCG
GGCTCGGCACCGGGTACATGAAGACCGAGTTCTTCGCGCTCGGAGTCGACATGGACGAGCGCAACACCCTGTTCGACGAG
ATCCTCGACGTGCTGCCGATGCACTGGAGCGGTGAGCCGTTCAGTTACAAGGGCCTGCACTTCGACGCCCGGAACGTCAT
CGCCCGGCCCCGACCGGTGCAGGACCCGATTCCCATCTGGATCGGCGGTAACTCGAAGCTGAGCCGGCGCCGCATCGCGC
AGCGGGCGCAGGGCTGGATGCCGCTGGTGGGCCCGCCGGAGCTCAGCGCGGCGGTGCGGACGACCTCCATCGGTTCGCTG
GGGGAGCTGGCCGTCATGATCAACGAGATCCGGGACGCCGCCGCGGCCGCCGGGCGGCCGGACACGATCGATGTGCAGTA
CTCCTACAAGGACCGCTCCATCGCGACGCCGACCGTCGAGCCCGATCGCCACCGTGAGGCGATCGCCGAGATCGAGAAGG
CCGGCGTGACCGCCGTGGTGGTGTCGAGCCCATCGGTGTCGTCCGCGGCCACGCTCGAGTTCCTCGAGGCGTTCGGCGCG
ACCTACCTGTCCTGA

Upstream 100 bases:

>100_bases
ACGGCGACCGTCAAGTTCCTCCTCAGCATCTAGGCCGAGCGACCTGAGTAGGCTCGCGCCCGGCCCCGGGCGATCGTGCT
CTCCCATGTCGGGTCAACCG

Downstream 100 bases:

>100_bases
CCGCGCCTGTCCTGACCGCGCCTGGCGAACGGGCCCGTATCGCGCGAGTCAGCGCTTGACGAGTAGATGCGGCGGGGGAG
GGCCGAAACGCCCGCGGACG

Product: luciferase family protein

Products: an aldehyde; SO3; H2O; FMN [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MCFESTPVDLPSHLLPTVPLDEVKMRFIYHYPETNGTDRDMLDAGALAEVAAAAERAGFYGFCLSEHPAPSARWLASGGH
QSLDPFVALGHVAAVTQRLRLHTNLSVAPYRNPFLLAKAAATVDKLSGGRIVLGLGTGYMKTEFFALGVDMDERNTLFDE
ILDVLPMHWSGEPFSYKGLHFDARNVIARPRPVQDPIPIWIGGNSKLSRRRIAQRAQGWMPLVGPPELSAAVRTTSIGSL
GELAVMINEIRDAAAAAGRPDTIDVQYSYKDRSIATPTVEPDRHREAIAEIEKAGVTAVVVSSPSVSSAATLEFLEAFGA
TYLS

Sequences:

>Translated_324_residues
MCFESTPVDLPSHLLPTVPLDEVKMRFIYHYPETNGTDRDMLDAGALAEVAAAAERAGFYGFCLSEHPAPSARWLASGGH
QSLDPFVALGHVAAVTQRLRLHTNLSVAPYRNPFLLAKAAATVDKLSGGRIVLGLGTGYMKTEFFALGVDMDERNTLFDE
ILDVLPMHWSGEPFSYKGLHFDARNVIARPRPVQDPIPIWIGGNSKLSRRRIAQRAQGWMPLVGPPELSAAVRTTSIGSL
GELAVMINEIRDAAAAAGRPDTIDVQYSYKDRSIATPTVEPDRHREAIAEIEKAGVTAVVVSSPSVSSAATLEFLEAFGA
TYLS
>Mature_324_residues
MCFESTPVDLPSHLLPTVPLDEVKMRFIYHYPETNGTDRDMLDAGALAEVAAAAERAGFYGFCLSEHPAPSARWLASGGH
QSLDPFVALGHVAAVTQRLRLHTNLSVAPYRNPFLLAKAAATVDKLSGGRIVLGLGTGYMKTEFFALGVDMDERNTLFDE
ILDVLPMHWSGEPFSYKGLHFDARNVIARPRPVQDPIPIWIGGNSKLSRRRIAQRAQGWMPLVGPPELSAAVRTTSIGSL
GELAVMINEIRDAAAAAGRPDTIDVQYSYKDRSIATPTVEPDRHREAIAEIEKAGVTAVVVSSPSVSSAATLEFLEAFGA
TYLS

Specific function: Involved In Desulfonation Of Aliphatic Sulfonates. Catalyzes The Conversion Of Pentanesulfonic Acid To Sulfite And Pentaldehyde And Is Able To Desulfonate A Wide Range Of Sulfonated Substrates Including C-2 To C-10 Unsubstituted Linear Alkanesulfonates, S

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To M.tuberculosis Rv2161c and Rv3079c [H]

Homologues:

Organism=Escherichia coli, GI1787166, Length=233, Percent_Identity=28.3261802575107, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019921
- InterPro:   IPR011251
- InterPro:   IPR016048 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: 1.14.14.5 [C]

Molecular weight: Translated: 35113; Mature: 35113

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCFESTPVDLPSHLLPTVPLDEVKMRFIYHYPETNGTDRDMLDAGALAEVAAAAERAGFY
CCCCCCCCCCCHHHCCCCCHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCE
GFCLSEHPAPSARWLASGGHQSLDPFVALGHVAAVTQRLRLHTNLSVAPYRNPFLLAKAA
EEECCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEHHHH
ATVDKLSGGRIVLGLGTGYMKTEFFALGVDMDERNTLFDEILDVLPMHWSGEPFSYKGLH
HHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEECCEE
FDARNVIARPRPVQDPIPIWIGGNSKLSRRRIAQRAQGWMPLVGPPELSAAVRTTSIGSL
ECHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCH
GELAVMINEIRDAAAAAGRPDTIDVQYSYKDRSIATPTVEPDRHREAIAEIEKAGVTAVV
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEE
VSSPSVSSAATLEFLEAFGATYLS
EECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCFESTPVDLPSHLLPTVPLDEVKMRFIYHYPETNGTDRDMLDAGALAEVAAAAERAGFY
CCCCCCCCCCCHHHCCCCCHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCE
GFCLSEHPAPSARWLASGGHQSLDPFVALGHVAAVTQRLRLHTNLSVAPYRNPFLLAKAA
EEECCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEHHHH
ATVDKLSGGRIVLGLGTGYMKTEFFALGVDMDERNTLFDEILDVLPMHWSGEPFSYKGLH
HHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEECCEE
FDARNVIARPRPVQDPIPIWIGGNSKLSRRRIAQRAQGWMPLVGPPELSAAVRTTSIGSL
ECHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCH
GELAVMINEIRDAAAAAGRPDTIDVQYSYKDRSIATPTVEPDRHREAIAEIEKAGVTAVV
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEE
VSSPSVSSAATLEFLEAFGATYLS
EECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: an alkanesulfonate; O2; FMNH2 [C]

Specific reaction: an alkanesulfonate + O2 + FMNH2 = an aldehyde + SO3 + H2O + FMN [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]