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The map label for this gene is rfbB [C]

Identifier: 158318391

GI number: 158318391

Start: 8084216

End: 8085295

Strand: Reverse

Name: rfbB [C]

Synonym: Franean1_6656

Alternate gene names: 158318391

Gene position: 8085295-8084216 (Counterclockwise)

Preceding gene: 158318392

Following gene: 158318390

Centisome position: 90.02

GC content: 71.3

Gene sequence:

>1080_bases
ATGAAGATAGCCGTCACGGGAGGATCCGGATTCATCGGCGGTCACGTCGTCGACCGCCTGCTCGACGCCGGCCACAAGGT
GCTGTCGCTCGACCTGAGCACCTGCGGGCGGCCCGACCCGCGTGCCGCCTACCGCGAGATCGACGTTCTCGACCTCGAGG
CGCTCGTCGACGCGTTCACGGGCGTGGACGTCGTCTTTCACATCGCCGGAATGTCGAACGTCGACTTCGCCTTCGCCGAC
CCGGTGCGCACGGTGCGCCTCAACGTGGAGGGAACCGGCAAGGTGTGCGAGGCGGCCCGGCGCACCGGCGTGCGCCGCGT
CGTGTTCGCGAGCACCGTCTGGGTCTACGGCGCCGTGGCCGACGTGCCCGCTCAGCCGCCCGCACAACCGACCGAGCAGC
CGACCGAGCAGCCGTCCGGGCTCGCGGCGGGCAGCGTCCCCGCTCCGGCCGGGCACGATGACCATCCGCGGCCCGCGCCG
CTCACCGAGGACGCCGAGATCGTGCTCGCGCGAGCCGGGCACGTGTACACCTCGACCAAGCTCGCGGCCGAGCTGCTCCT
GCACAGCTACCAGCAGACCTACGGGCTCCCGTTCACGATCCTGCGGTACGGCATCCCCTACGGCCCCGGCATGCGTGACG
AGCTGGTGCTGGCCAGGTTCGTCCACAATGCCGTGCACGGCCGGCCGCTGACCGTCGCCGGCGACGGCCTGCAGTTCCGC
AACTACGTCTACGTCCGTGATCTGGCCGACGCGCACGTGCTCGCGCTCGCCGACTCCGCGGCGAACACCACCCTCGCCCT
GGAGGGCAACGAGCCGGTGAGCGTGCTGGAGATGGCCCAGGCCGTCCAGCGGCACTTCCCCGGGACGGAGATTGAGCACA
TGCCAGCCCGGCCGGGGGACTTCCGCGGCCGGGAGATCTCCGCGCAGCGGGCCGCCGGCCTTCTCGGCTGGCGGCCGACG
ACGCCGTTCCTGGACGGCGTCCGGCAGTACATCGAGTGGTATCAGGCGAACCGGCAGCCACCCGCGGTGACTGCGCCGTC
GATCGGGGATCTCGGCGTCTCCGCGACCTCATCGGAATGA

Upstream 100 bases:

>100_bases
GCCTGCCACTGCATTCCGACATGACCGACGCCGAGGCCGAACATGTCATCTCGGCATTCCGTGCCGTCGTCCACGGAATG
GAACTGGACGGTGCCCGGCG

Downstream 100 bases:

>100_bases
GCGTGTCGAAGATGGACGGGATCGAGGGGATCATGGACGACGTCACGGCGGAGAGCGAGCCCGAGTCTCGGCGCGGGAAC
GTGCTGCTGCTCTCCGGCTC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MKIAVTGGSGFIGGHVVDRLLDAGHKVLSLDLSTCGRPDPRAAYREIDVLDLEALVDAFTGVDVVFHIAGMSNVDFAFAD
PVRTVRLNVEGTGKVCEAARRTGVRRVVFASTVWVYGAVADVPAQPPAQPTEQPTEQPSGLAAGSVPAPAGHDDHPRPAP
LTEDAEIVLARAGHVYTSTKLAAELLLHSYQQTYGLPFTILRYGIPYGPGMRDELVLARFVHNAVHGRPLTVAGDGLQFR
NYVYVRDLADAHVLALADSAANTTLALEGNEPVSVLEMAQAVQRHFPGTEIEHMPARPGDFRGREISAQRAAGLLGWRPT
TPFLDGVRQYIEWYQANRQPPAVTAPSIGDLGVSATSSE

Sequences:

>Translated_359_residues
MKIAVTGGSGFIGGHVVDRLLDAGHKVLSLDLSTCGRPDPRAAYREIDVLDLEALVDAFTGVDVVFHIAGMSNVDFAFAD
PVRTVRLNVEGTGKVCEAARRTGVRRVVFASTVWVYGAVADVPAQPPAQPTEQPTEQPSGLAAGSVPAPAGHDDHPRPAP
LTEDAEIVLARAGHVYTSTKLAAELLLHSYQQTYGLPFTILRYGIPYGPGMRDELVLARFVHNAVHGRPLTVAGDGLQFR
NYVYVRDLADAHVLALADSAANTTLALEGNEPVSVLEMAQAVQRHFPGTEIEHMPARPGDFRGREISAQRAAGLLGWRPT
TPFLDGVRQYIEWYQANRQPPAVTAPSIGDLGVSATSSE
>Mature_359_residues
MKIAVTGGSGFIGGHVVDRLLDAGHKVLSLDLSTCGRPDPRAAYREIDVLDLEALVDAFTGVDVVFHIAGMSNVDFAFAD
PVRTVRLNVEGTGKVCEAARRTGVRRVVFASTVWVYGAVADVPAQPPAQPTEQPTEQPSGLAAGSVPAPAGHDDHPRPAP
LTEDAEIVLARAGHVYTSTKLAAELLLHSYQQTYGLPFTILRYGIPYGPGMRDELVLARFVHNAVHGRPLTVAGDGLQFR
NYVYVRDLADAHVLALADSAANTTLALEGNEPVSVLEMAQAVQRHFPGTEIEHMPARPGDFRGREISAQRAAGLLGWRPT
TPFLDGVRQYIEWYQANRQPPAVTAPSIGDLGVSATSSE

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=359, Percent_Identity=26.1838440111421, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI42516563, Length=350, Percent_Identity=27.7142857142857, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI310132178, Length=268, Percent_Identity=28.3582089552239, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI310113012, Length=268, Percent_Identity=28.3582089552239, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI239745448, Length=268, Percent_Identity=28.3582089552239, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788353, Length=368, Percent_Identity=26.9021739130435, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI48994969, Length=376, Percent_Identity=25.7978723404255, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17539532, Length=342, Percent_Identity=25.7309941520468, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17568069, Length=359, Percent_Identity=23.3983286908078, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI115532424, Length=355, Percent_Identity=25.9154929577465, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6319493, Length=369, Percent_Identity=24.1192411924119, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356223, Length=342, Percent_Identity=26.3157894736842, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19923002, Length=349, Percent_Identity=25.214899713467, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 38466; Mature: 38466

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVTGGSGFIGGHVVDRLLDAGHKVLSLDLSTCGRPDPRAAYREIDVLDLEALVDAFT
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEEHHHCCCCCCHHHHHHCCHHHHHHHHHHHC
GVDVVFHIAGMSNVDFAFADPVRTVRLNVEGTGKVCEAARRTGVRRVVFASTVWVYGAVA
CCEEEHEECCCCCCCEEECCCEEEEEEEECCCCHHHHHHHHCCCEEEHHHHHHHHHHHHH
DVPAQPPAQPTEQPTEQPSGLAAGSVPAPAGHDDHPRPAPLTEDAEIVLARAGHVYTSTK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEHHHH
LAAELLLHSYQQTYGLPFTILRYGIPYGPGMRDELVLARFVHNAVHGRPLTVAGDGLQFR
HHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEEC
NYVYVRDLADAHVLALADSAANTTLALEGNEPVSVLEMAQAVQRHFPGTEIEHMPARPGD
CEEEEEECCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
FRGREISAQRAAGLLGWRPTTPFLDGVRQYIEWYQANRQPPAVTAPSIGDLGVSATSSE
CCCCEECHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIAVTGGSGFIGGHVVDRLLDAGHKVLSLDLSTCGRPDPRAAYREIDVLDLEALVDAFT
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEEHHHCCCCCCHHHHHHCCHHHHHHHHHHHC
GVDVVFHIAGMSNVDFAFADPVRTVRLNVEGTGKVCEAARRTGVRRVVFASTVWVYGAVA
CCEEEHEECCCCCCCEEECCCEEEEEEEECCCCHHHHHHHHCCCEEEHHHHHHHHHHHHH
DVPAQPPAQPTEQPTEQPSGLAAGSVPAPAGHDDHPRPAPLTEDAEIVLARAGHVYTSTK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEHHHH
LAAELLLHSYQQTYGLPFTILRYGIPYGPGMRDELVLARFVHNAVHGRPLTVAGDGLQFR
HHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEEC
NYVYVRDLADAHVLALADSAANTTLALEGNEPVSVLEMAQAVQRHFPGTEIEHMPARPGD
CEEEEEECCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
FRGREISAQRAAGLLGWRPTTPFLDGVRQYIEWYQANRQPPAVTAPSIGDLGVSATSSE
CCCCEECHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]