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The map label for this gene is stp [H]

Identifier: 158318319

GI number: 158318319

Start: 8012495

End: 8014003

Strand: Reverse

Name: stp [H]

Synonym: Franean1_6584

Alternate gene names: 158318319

Gene position: 8014003-8012495 (Counterclockwise)

Preceding gene: 158318320

Following gene: 158318315

Centisome position: 89.22

GC content: 73.03

Gene sequence:

>1509_bases
ATGGCCGAGCCGACCAGTCGCCGCAGGTTCGCCGTTCTCGCGATCTGCTGCATGAGTCTGCTGATCGTGGGGCTGGACGG
CACCATCGTGAACGTGGCCCTGCCCGCACTGCGCCGGGATCTGGGTGCGTCGCCCGCGGGCCTGCAATGGACGATCGACA
GCTACACCCTCGTCCTGGCCAGCCTGCTCATGCTCGGTGGTTCCACCGGCGACCGGCTCGGCCGCCGTCGGATCTTCCAG
ACCGGACTGGCCCTGTTCACCGCAGGCTCGCTGCTGTGCAGCCTCGCGCCCGGGCTCGGCTGGCTGGTGGTCTTCCGAAT
GGTGCAGGCCGTCGGCGGCTCGATGCTGAACCCGGTTGCGATGTCCATCATCACCAACACGTTCACCGACCCGCGGGAAC
GGGCCCGCGCCATCGGTCTCTGGGGCGCGGTGATCGGGATCAGCATGGCACTCGGCCCGGTGCTCGGCGGCATCCTCGTC
GAGACGGTGAGCTGGCGGGCGATCTTCTGGATCAACATCCCGATCGGGCTCGCCGCGCTGGTGCTCACGCACCGCTTCGT
CCCCGAGTCCCGCGCGCCGAGCCCACGCCGCCTGGACCCCGTCGGACAGCTCCTTGTGATCGCCGTCCTCGCGACCCTCA
CCTACTCGATTATCGAGGCGCCCGCGGCAGGCTGGACGTCCGCCGAAACGCTCGTCCTGCTCGCGGTGGCGGTCGCCGCG
GCGGCGCTGCTGGTCGGCTACGAGTCGCGGCGCACCGAGCCGCTGCTGGACGTCCGCTTCTTCCGCAGCGCGCCCTTCTC
CGGCGCGACGGCGATCGCGGTGTGCGCGTTCGGCGCCCTCGCCGGCTTCCTCTTCCTCAACACGATCTACCTGCAGGACG
TCCGTGGCTACTCGGCGCTGCACGCCGGCCTGCTGACCCTGCCGATGGCGCTGGCCACCCTGGTGATCGCGCCCCTGTCC
GGCCGGGCGGTGGGCCGCTTCGGCCCGCGTCCGTCGCTGCTCGCCGCGGGTGTCGCCCTGACCGCCGGCAGCGCGGCGAT
GACGGGCCTGTCCCCACAGACCTCCGTCACCACCCTGATGGTCGCCTACGTCCTCTTCGGGACGGGCTTCGGCCTGGTGA
ACGCGCCGATCACCAACACGGCGGTCTCCGGGATGCCGCTCGAGCAGGCCGGGGTCGCGGCGGCGGTCGCCTCGACGAGC
CGCCAGGTCGGCCAGTCGCTGGGCGTGGCCGTGATCGGCTCCGTCGCGGCAGCGACCGCGACGGTCGGGGCCGTCGACCC
CACCAGCACGGCCGCCTGGTGGGCGGTGGTCGCGGCCTGCGCCGCCGTCCTCGCCCTGGGGTTCGCGACGACGGGCACCT
GGGCCCGGCGCACCGCGGACCAGACCCGGTCCCGCCTCCTGACCACCCTCGCCCCCGGGCAGGCCGCCACCCCGCAGGAG
GGGACCCAGCAGGCTGGGAACCAGCAGGCCGCCCACCCGGTGACCGCCGAACCGGAGCCCAGCCCCTGA

Upstream 100 bases:

>100_bases
CGAGCGCGAGCGCGAGGAAAAGAGACCTGCCGTACGCTGAACAGTCGTCCGCCCGCGCACCTGCGGCCGAGTTGGCGGCG
GCCGGAGAGGTGGGTTGTCG

Downstream 100 bases:

>100_bases
CCCGCCACCCGCCCGGCGCCGCGCCCGCGCTCTGACGATCAGCCCGTGTTGCGGAGGCCGGCGGCTATTCCATTGATGGT
CAGTAGGAGGCCGCGGCGTA

Product: EmrB/QacA family drug resistance transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MAEPTSRRRFAVLAICCMSLLIVGLDGTIVNVALPALRRDLGASPAGLQWTIDSYTLVLASLLMLGGSTGDRLGRRRIFQ
TGLALFTAGSLLCSLAPGLGWLVVFRMVQAVGGSMLNPVAMSIITNTFTDPRERARAIGLWGAVIGISMALGPVLGGILV
ETVSWRAIFWINIPIGLAALVLTHRFVPESRAPSPRRLDPVGQLLVIAVLATLTYSIIEAPAAGWTSAETLVLLAVAVAA
AALLVGYESRRTEPLLDVRFFRSAPFSGATAIAVCAFGALAGFLFLNTIYLQDVRGYSALHAGLLTLPMALATLVIAPLS
GRAVGRFGPRPSLLAAGVALTAGSAAMTGLSPQTSVTTLMVAYVLFGTGFGLVNAPITNTAVSGMPLEQAGVAAAVASTS
RQVGQSLGVAVIGSVAAATATVGAVDPTSTAAWWAVVAACAAVLALGFATTGTWARRTADQTRSRLLTTLAPGQAATPQE
GTQQAGNQQAAHPVTAEPEPSP

Sequences:

>Translated_502_residues
MAEPTSRRRFAVLAICCMSLLIVGLDGTIVNVALPALRRDLGASPAGLQWTIDSYTLVLASLLMLGGSTGDRLGRRRIFQ
TGLALFTAGSLLCSLAPGLGWLVVFRMVQAVGGSMLNPVAMSIITNTFTDPRERARAIGLWGAVIGISMALGPVLGGILV
ETVSWRAIFWINIPIGLAALVLTHRFVPESRAPSPRRLDPVGQLLVIAVLATLTYSIIEAPAAGWTSAETLVLLAVAVAA
AALLVGYESRRTEPLLDVRFFRSAPFSGATAIAVCAFGALAGFLFLNTIYLQDVRGYSALHAGLLTLPMALATLVIAPLS
GRAVGRFGPRPSLLAAGVALTAGSAAMTGLSPQTSVTTLMVAYVLFGTGFGLVNAPITNTAVSGMPLEQAGVAAAVASTS
RQVGQSLGVAVIGSVAAATATVGAVDPTSTAAWWAVVAACAAVLALGFATTGTWARRTADQTRSRLLTTLAPGQAATPQE
GTQQAGNQQAAHPVTAEPEPSP
>Mature_501_residues
AEPTSRRRFAVLAICCMSLLIVGLDGTIVNVALPALRRDLGASPAGLQWTIDSYTLVLASLLMLGGSTGDRLGRRRIFQT
GLALFTAGSLLCSLAPGLGWLVVFRMVQAVGGSMLNPVAMSIITNTFTDPRERARAIGLWGAVIGISMALGPVLGGILVE
TVSWRAIFWINIPIGLAALVLTHRFVPESRAPSPRRLDPVGQLLVIAVLATLTYSIIEAPAAGWTSAETLVLLAVAVAAA
ALLVGYESRRTEPLLDVRFFRSAPFSGATAIAVCAFGALAGFLFLNTIYLQDVRGYSALHAGLLTLPMALATLVIAPLSG
RAVGRFGPRPSLLAAGVALTAGSAAMTGLSPQTSVTTLMVAYVLFGTGFGLVNAPITNTAVSGMPLEQAGVAAAVASTSR
QVGQSLGVAVIGSVAAATATVGAVDPTSTAAWWAVVAACAAVLALGFATTGTWARRTADQTRSRLLTTLAPGQAATPQEG
TQQAGNQQAAHPVTAEPEPSP

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=417, Percent_Identity=32.3741007194245, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1790195, Length=422, Percent_Identity=31.9905213270142, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1789042, Length=422, Percent_Identity=26.5402843601896, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1788710, Length=423, Percent_Identity=27.6595744680851, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788392, Length=418, Percent_Identity=32.7751196172249, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790146, Length=188, Percent_Identity=31.3829787234043, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324954, Length=410, Percent_Identity=24.6341463414634, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323520, Length=404, Percent_Identity=23.019801980198, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=203, Percent_Identity=28.0788177339901, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323735, Length=308, Percent_Identity=24.6753246753247, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=151, Percent_Identity=27.1523178807947, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=164, Percent_Identity=29.2682926829268, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51587; Mature: 51456

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPTSRRRFAVLAICCMSLLIVGLDGTIVNVALPALRRDLGASPAGLQWTIDSYTLVLA
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHH
SLLMLGGSTGDRLGRRRIFQTGLALFTAGSLLCSLAPGLGWLVVFRMVQAVGGSMLNPVA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHH
MSIITNTFTDPRERARAIGLWGAVIGISMALGPVLGGILVETVSWRAIFWINIPIGLAAL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHH
VLTHRFVPESRAPSPRRLDPVGQLLVIAVLATLTYSIIEAPAAGWTSAETLVLLAVAVAA
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
AALLVGYESRRTEPLLDVRFFRSAPFSGATAIAVCAFGALAGFLFLNTIYLQDVRGYSAL
HHHHHCCHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
HAGLLTLPMALATLVIAPLSGRAVGRFGPRPSLLAAGVALTAGSAAMTGLSPQTSVTTLM
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHH
VAYVLFGTGFGLVNAPITNTAVSGMPLEQAGVAAAVASTSRQVGQSLGVAVIGSVAAATA
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVGAVDPTSTAAWWAVVAACAAVLALGFATTGTWARRTADQTRSRLLTTLAPGQAATPQE
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCH
GTQQAGNQQAAHPVTAEPEPSP
HHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AEPTSRRRFAVLAICCMSLLIVGLDGTIVNVALPALRRDLGASPAGLQWTIDSYTLVLA
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHH
SLLMLGGSTGDRLGRRRIFQTGLALFTAGSLLCSLAPGLGWLVVFRMVQAVGGSMLNPVA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHH
MSIITNTFTDPRERARAIGLWGAVIGISMALGPVLGGILVETVSWRAIFWINIPIGLAAL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHH
VLTHRFVPESRAPSPRRLDPVGQLLVIAVLATLTYSIIEAPAAGWTSAETLVLLAVAVAA
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
AALLVGYESRRTEPLLDVRFFRSAPFSGATAIAVCAFGALAGFLFLNTIYLQDVRGYSAL
HHHHHCCHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
HAGLLTLPMALATLVIAPLSGRAVGRFGPRPSLLAAGVALTAGSAAMTGLSPQTSVTTLM
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHH
VAYVLFGTGFGLVNAPITNTAVSGMPLEQAGVAAAVASTSRQVGQSLGVAVIGSVAAATA
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVGAVDPTSTAAWWAVVAACAAVLALGFATTGTWARRTADQTRSRLLTTLAPGQAATPQE
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCH
GTQQAGNQQAAHPVTAEPEPSP
HHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]