Click here to switch to the map view.

The map label for this gene is gsiB [H]

Identifier: 158318094

GI number: 158318094

Start: 7718556

End: 7720115

Strand: Reverse

Name: gsiB [H]

Synonym: Franean1_6358

Alternate gene names: 158318094

Gene position: 7720115-7718556 (Counterclockwise)

Preceding gene: 158318095

Following gene: 158318092

Centisome position: 85.95

GC content: 67.18

Gene sequence:

>1560_bases
GTGATCCCCAAAGTACGATTACTCGTCACCGCTGCCGTCTGTTGCGCAACGCTGGCCCTGGGCGCCTGCGGGGGAGGTGG
CGACACCGGCCCGTCCTCGGGTGCCTCCGGCGAACCGGTTGCCGGTGGCGAGGGAAGAATTCTCACCCTCAGCGATCCGC
GCAGCCTCGACCCGGCGGCTCTCGGCAACGCCTACGCAACCACCGGTGTTGTGGGAAACGCGCTGTACGGAACGCTGATG
ACCGACCCGGGCGGCAAAATACGGTACTCGATGGCCGAGTCCTTCCAGACCACCGACGCCGGGGCGACATTCGAGCTGAA
ACTGCGGTCGGGTCTGGTGTTCTCCGACGGAACCTCACTGGACGCCGAAGCCGTGAAGTTCAACTGGGACAGGCTCAAGA
ACCCGGCCACCGCCGCCATCTCCCGGTCGGAGGCGTCGATGATCGCCTCATCCGACGTGGTCGATGACACCACCTTGAAG
ATCACCATGGCCACGCCGGTGCCGAAGTACGCCCAAGCCGTCCTCACCTCGTCCCTGAACTGGATCGCCTCGCCGACCGC
CCTGGAGAAGGGGCCGCAGGCCTTCGACGCGAACCCGATCGGCGCCGGGCCATTCACCCTGCGGAGCTGGACACGCCAGG
CCGCCATGGAACTGGTCAAGAACCCCCGCTACTGGGACGCCCCCAAGCCCTACCTCGACCGTCTCACCCTCCGCGCGGCC
CTCGACAGCAGCCAGCGCTACAACACGGTGCTCACCGGGGGCGCGGACGCGGCCGTCGAGTCGAGCTGGGTCAACCTCGA
CAAAGCCGAGCAGGCCGGCCTGCCGACGAACCTCATACCGACCGGCGGCGGCATCTTCATGGCGCTGAACACACGCAGGG
CACCCTTCGACGACGTCCGCGCCCGCCAGGCCCTCGCCGCGGCAATCGACAGGGACGCACTCAACCAGGCTGTCTACAGC
GGGACCGGCGAGCCCGTCGACACACTGTTCAGTAAGGACTCTCCTTACTACTCGGACACGCCGCTGGCGACAACGGACCG
TGCACGGGCGCAACGGCTCCTCGACGAGCTGGCCGCCGACGGCAAACCGCTGTCCTTCGTCTTCTCCAGCGTCCCCACGA
CGGATGGCAAGGCGATCGCGGAGAACATCCAGGCCCAGCTCAGCAGCTTCAAGAACGTCACCGTCAAGATCAAGACCATC
GAGGTCGCGGAGCTGGCCGCGCTGCGCACCACCCACGACTTCGACGTGCTCGTCTCGTCGGCCTTCTTCCGGGACCCCGA
ACCGCGGCTGTGGACGACCTTCCACGGGACCTCGGCGGCGAACCTGCCCGGCATCAACGACCCGGCACTCAACGAAAGCC
TCGCGGCCGCGCGCACCGCGACTTCGGAGCCCGAGCGCGAATCCGCCTACGGGACACTGCAGGAACGGCTGGCAGAGCTG
ACCCCGGTGGTCTTCCTCGCGCAGGCGGCACCCAGCGCCTTCTCGAGCAAGAACGTCGGCGGACTCGTACAGTACGGCCT
CGGCTCACTTCAGCCCGAGGAACTCTGGATTCAGCGCTAG

Upstream 100 bases:

>100_bases
AACACCTCATGTCACTGCCGTCCGCCCGGACCCGCGCTCGCCCAGGTCTTTTTGGCTCCGTCCCTTTTCATCTGTCTCGA
ACGTTGACAAGAGGTCCGTT

Downstream 100 bases:

>100_bases
ACAGAGTCCCCCGAGCATCCGCTGACCGGGAGGGGGCCACGTGGAACCACTGCGAAGGACCGAACCGGCGACGGCAGCAG
CCTCACTTCGTCTCGGGATC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MIPKVRLLVTAAVCCATLALGACGGGGDTGPSSGASGEPVAGGEGRILTLSDPRSLDPAALGNAYATTGVVGNALYGTLM
TDPGGKIRYSMAESFQTTDAGATFELKLRSGLVFSDGTSLDAEAVKFNWDRLKNPATAAISRSEASMIASSDVVDDTTLK
ITMATPVPKYAQAVLTSSLNWIASPTALEKGPQAFDANPIGAGPFTLRSWTRQAAMELVKNPRYWDAPKPYLDRLTLRAA
LDSSQRYNTVLTGGADAAVESSWVNLDKAEQAGLPTNLIPTGGGIFMALNTRRAPFDDVRARQALAAAIDRDALNQAVYS
GTGEPVDTLFSKDSPYYSDTPLATTDRARAQRLLDELAADGKPLSFVFSSVPTTDGKAIAENIQAQLSSFKNVTVKIKTI
EVAELAALRTTHDFDVLVSSAFFRDPEPRLWTTFHGTSAANLPGINDPALNESLAAARTATSEPERESAYGTLQERLAEL
TPVVFLAQAAPSAFSSKNVGGLVQYGLGSLQPEELWIQR

Sequences:

>Translated_519_residues
MIPKVRLLVTAAVCCATLALGACGGGGDTGPSSGASGEPVAGGEGRILTLSDPRSLDPAALGNAYATTGVVGNALYGTLM
TDPGGKIRYSMAESFQTTDAGATFELKLRSGLVFSDGTSLDAEAVKFNWDRLKNPATAAISRSEASMIASSDVVDDTTLK
ITMATPVPKYAQAVLTSSLNWIASPTALEKGPQAFDANPIGAGPFTLRSWTRQAAMELVKNPRYWDAPKPYLDRLTLRAA
LDSSQRYNTVLTGGADAAVESSWVNLDKAEQAGLPTNLIPTGGGIFMALNTRRAPFDDVRARQALAAAIDRDALNQAVYS
GTGEPVDTLFSKDSPYYSDTPLATTDRARAQRLLDELAADGKPLSFVFSSVPTTDGKAIAENIQAQLSSFKNVTVKIKTI
EVAELAALRTTHDFDVLVSSAFFRDPEPRLWTTFHGTSAANLPGINDPALNESLAAARTATSEPERESAYGTLQERLAEL
TPVVFLAQAAPSAFSSKNVGGLVQYGLGSLQPEELWIQR
>Mature_519_residues
MIPKVRLLVTAAVCCATLALGACGGGGDTGPSSGASGEPVAGGEGRILTLSDPRSLDPAALGNAYATTGVVGNALYGTLM
TDPGGKIRYSMAESFQTTDAGATFELKLRSGLVFSDGTSLDAEAVKFNWDRLKNPATAAISRSEASMIASSDVVDDTTLK
ITMATPVPKYAQAVLTSSLNWIASPTALEKGPQAFDANPIGAGPFTLRSWTRQAAMELVKNPRYWDAPKPYLDRLTLRAA
LDSSQRYNTVLTGGADAAVESSWVNLDKAEQAGLPTNLIPTGGGIFMALNTRRAPFDDVRARQALAAAIDRDALNQAVYS
GTGEPVDTLFSKDSPYYSDTPLATTDRARAQRLLDELAADGKPLSFVFSSVPTTDGKAIAENIQAQLSSFKNVTVKIKTI
EVAELAALRTTHDFDVLVSSAFFRDPEPRLWTTFHGTSAANLPGINDPALNESLAAARTATSEPERESAYGTLQERLAEL
TPVVFLAQAAPSAFSSKNVGGLVQYGLGSLQPEELWIQR

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=325, Percent_Identity=29.5384615384615, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI87081878, Length=511, Percent_Identity=26.2230919765166, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1789397, Length=395, Percent_Identity=26.8354430379747, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787495, Length=481, Percent_Identity=24.7401247401247, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789966, Length=464, Percent_Identity=25, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787762, Length=475, Percent_Identity=24, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1789887, Length=316, Percent_Identity=25.3164556962025, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787551, Length=491, Percent_Identity=23.4215885947047, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 54862; Mature: 54862

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPKVRLLVTAAVCCATLALGACGGGGDTGPSSGASGEPVAGGEGRILTLSDPRSLDPAA
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHH
LGNAYATTGVVGNALYGTLMTDPGGKIRYSMAESFQTTDAGATFELKLRSGLVFSDGTSL
CCCCHHHHHHHHHHHEEEEEECCCCCEEEHHHHCCCCCCCCCEEEEEECCCEEEECCCCC
DAEAVKFNWDRLKNPATAAISRSEASMIASSDVVDDTTLKITMATPVPKYAQAVLTSSLN
CCHHEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCH
WIASPTALEKGPQAFDANPIGAGPFTLRSWTRQAAMELVKNPRYWDAPKPYLDRLTLRAA
HHCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LDSSQRYNTVLTGGADAAVESSWVNLDKAEQAGLPTNLIPTGGGIFMALNTRRAPFDDVR
HCCCCCCCEEEECCCCHHHHHHHCCCCCHHHCCCCCCEEECCCCEEEEEECCCCCHHHHH
ARQALAAAIDRDALNQAVYSGTGEPVDTLFSKDSPYYSDTPLATTDRARAQRLLDELAAD
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
GKPLSFVFSSVPTTDGKAIAENIQAQLSSFKNVTVKIKTIEVAELAALRTTHDFDVLVSS
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHCCCHHHHHHHH
AFFRDPEPRLWTTFHGTSAANLPGINDPALNESLAAARTATSEPERESAYGTLQERLAEL
HHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
TPVVFLAQAAPSAFSSKNVGGLVQYGLGSLQPEELWIQR
HHHHHEECCCCCCCCCCCCCCHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure
MIPKVRLLVTAAVCCATLALGACGGGGDTGPSSGASGEPVAGGEGRILTLSDPRSLDPAA
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHH
LGNAYATTGVVGNALYGTLMTDPGGKIRYSMAESFQTTDAGATFELKLRSGLVFSDGTSL
CCCCHHHHHHHHHHHEEEEEECCCCCEEEHHHHCCCCCCCCCEEEEEECCCEEEECCCCC
DAEAVKFNWDRLKNPATAAISRSEASMIASSDVVDDTTLKITMATPVPKYAQAVLTSSLN
CCHHEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCH
WIASPTALEKGPQAFDANPIGAGPFTLRSWTRQAAMELVKNPRYWDAPKPYLDRLTLRAA
HHCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LDSSQRYNTVLTGGADAAVESSWVNLDKAEQAGLPTNLIPTGGGIFMALNTRRAPFDDVR
HCCCCCCCEEEECCCCHHHHHHHCCCCCHHHCCCCCCEEECCCCEEEEEECCCCCHHHHH
ARQALAAAIDRDALNQAVYSGTGEPVDTLFSKDSPYYSDTPLATTDRARAQRLLDELAAD
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
GKPLSFVFSSVPTTDGKAIAENIQAQLSSFKNVTVKIKTIEVAELAALRTTHDFDVLVSS
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHCCCHHHHHHHH
AFFRDPEPRLWTTFHGTSAANLPGINDPALNESLAAARTATSEPERESAYGTLQERLAEL
HHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
TPVVFLAQAAPSAFSSKNVGGLVQYGLGSLQPEELWIQR
HHHHHEECCCCCCCCCCCCCCHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA