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The map label for this gene is cysA [H]

Identifier: 158317911

GI number: 158317911

Start: 7500121

End: 7500954

Strand: Reverse

Name: cysA [H]

Synonym: Franean1_6169

Alternate gene names: 158317911

Gene position: 7500954-7500121 (Counterclockwise)

Preceding gene: 158317912

Following gene: 158317910

Centisome position: 83.51

GC content: 67.15

Gene sequence:

>834_bases
ATGAGCCGTGAGAACGCGCTGGTCGACGCGACCTGGGTGGACGCCCACAGCGGCGACCCCAAGGTCGTCCTCGTCGAGGT
CGACGAGGACGTGTCGGCCTACGACAAGGGCCACATCCCCGGCGCAGTGCGGCTTGACTGGAAGTCCGAACTGCAGGACC
CGGTGATCCGTGACTTCGTGAACAAGGAGCAGTTCGAGAAGCTCCTGTCGGAGAAGGGCATCGCCGACGATGACACCGTG
GTGCTCTACGGCGGCAACAACAACTGGTTCGCCGCCTACGCCTACTGGTACTTCGTCCTCTACGGCCACGCCGAGGTCCG
CCTGCTCGACGGCGGCCGCAAGAAGTGGGAGCTGGACGGCCGTGAGCTGACCATCGAGGTCACCCCGCGCGCGGCGACCA
AGTACTCCGCCTCGGAGGCGCGTACCGACATCCGCGCGTTCCGTGACGAGGTCATCACCGCCATCGGTGACAAGAACCTG
GTGGACGTCCGCTCGCCCGACGAGTTCAGCGGCCGCCTGCTCGCCCCGGCGCACCTGCCGCAGGAGCAGTCGCAGCGGGC
CGGCCACGTGCCGACCGCGAAGAACATCCCGTGGGCGAGGACCGCCAACGAGGACGGCACCTTCAAGTCGAACGCCGACC
TGGAGAAGCTGTACTCCGAGGCCGGTGTCGACCTGTCGAAGGACACCATCGCCTACTGCCGGATCGGGGAGCGCTCCGCG
CACACCTGGTTCGCCCTGCACGAGCTGCTGGGCCTGGCGAACGTCAAGAACTACGACGGCTCGTGGACCGAGTACGGCTC
CCTGGTGGGCGTGCCGATCGAGAAGGGTGCCTGA

Upstream 100 bases:

>100_bases
TCGCCTTCGCCGCGGCCTTCCTGAACGCGGCGTTCGGATTCTGCCTCGGATGCCAGACCTACCTCGTCCTACGTTCCGCA
ACTGTGAAGGGAGCACGCAC

Downstream 100 bases:

>100_bases
TCATGTGTGGCGCTACCAGTGGTGGACCCTCGGTGGAAGGAATCGACGTGGCCAAGGAGACCGTGATCCAGGGCGTCGTG
GTGAAGGACGGGTCGCCGGT

Product: rhodanese domain-containing protein

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSRENALVDATWVDAHSGDPKVVLVEVDEDVSAYDKGHIPGAVRLDWKSELQDPVIRDFVNKEQFEKLLSEKGIADDDTV
VLYGGNNNWFAAYAYWYFVLYGHAEVRLLDGGRKKWELDGRELTIEVTPRAATKYSASEARTDIRAFRDEVITAIGDKNL
VDVRSPDEFSGRLLAPAHLPQEQSQRAGHVPTAKNIPWARTANEDGTFKSNADLEKLYSEAGVDLSKDTIAYCRIGERSA
HTWFALHELLGLANVKNYDGSWTEYGSLVGVPIEKGA

Sequences:

>Translated_277_residues
MSRENALVDATWVDAHSGDPKVVLVEVDEDVSAYDKGHIPGAVRLDWKSELQDPVIRDFVNKEQFEKLLSEKGIADDDTV
VLYGGNNNWFAAYAYWYFVLYGHAEVRLLDGGRKKWELDGRELTIEVTPRAATKYSASEARTDIRAFRDEVITAIGDKNL
VDVRSPDEFSGRLLAPAHLPQEQSQRAGHVPTAKNIPWARTANEDGTFKSNADLEKLYSEAGVDLSKDTIAYCRIGERSA
HTWFALHELLGLANVKNYDGSWTEYGSLVGVPIEKGA
>Mature_276_residues
SRENALVDATWVDAHSGDPKVVLVEVDEDVSAYDKGHIPGAVRLDWKSELQDPVIRDFVNKEQFEKLLSEKGIADDDTVV
LYGGNNNWFAAYAYWYFVLYGHAEVRLLDGGRKKWELDGRELTIEVTPRAATKYSASEARTDIRAFRDEVITAIGDKNLV
DVRSPDEFSGRLLAPAHLPQEQSQRAGHVPTAKNIPWARTANEDGTFKSNADLEKLYSEAGVDLSKDTIAYCRIGERSAH
TWFALHELLGLANVKNYDGSWTEYGSLVGVPIEKGA

Specific function: May be a sulfotransferase involved in the formation of thiosulfate [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=239, Percent_Identity=30.5439330543933, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI194473668, Length=278, Percent_Identity=27.3381294964029, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI61835204, Length=278, Percent_Identity=27.3381294964029, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI194473681, Length=278, Percent_Identity=27.3381294964029, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI87082121, Length=288, Percent_Identity=28.125, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI87081967, Length=275, Percent_Identity=28.3636363636364, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17561888, Length=274, Percent_Identity=27.3722627737226, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71997283, Length=278, Percent_Identity=27.6978417266187, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI115534702, Length=247, Percent_Identity=29.5546558704453, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17559150, Length=267, Percent_Identity=24.3445692883895, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324825, Length=280, Percent_Identity=27.5, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 30902; Mature: 30770

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRENALVDATWVDAHSGDPKVVLVEVDEDVSAYDKGHIPGAVRLDWKSELQDPVIRDFV
CCCCCEEEEEEEEECCCCCCEEEEEEECCCCCHHCCCCCCCEEEECCHHHHCCHHHHHHC
NKEQFEKLLSEKGIADDDTVVLYGGNNNWFAAYAYWYFVLYGHAEVRLLDGGRKKWELDG
CHHHHHHHHHHCCCCCCCEEEEECCCCCEEEHHHEEEEHEECCCEEEEECCCCEEEEECC
RELTIEVTPRAATKYSASEARTDIRAFRDEVITAIGDKNLVDVRSPDEFSGRLLAPAHLP
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEECCCCCC
QEQSQRAGHVPTAKNIPWARTANEDGTFKSNADLEKLYSEAGVDLSKDTIAYCRIGERSA
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCC
HTWFALHELLGLANVKNYDGSWTEYGSLVGVPIEKGA
HHHHHHHHHHHHHCCCCCCCCCHHCCCEEECCCCCCC
>Mature Secondary Structure 
SRENALVDATWVDAHSGDPKVVLVEVDEDVSAYDKGHIPGAVRLDWKSELQDPVIRDFV
CCCCEEEEEEEEECCCCCCEEEEEEECCCCCHHCCCCCCCEEEECCHHHHCCHHHHHHC
NKEQFEKLLSEKGIADDDTVVLYGGNNNWFAAYAYWYFVLYGHAEVRLLDGGRKKWELDG
CHHHHHHHHHHCCCCCCCEEEEECCCCCEEEHHHEEEEHEECCCEEEEECCCCEEEEECC
RELTIEVTPRAATKYSASEARTDIRAFRDEVITAIGDKNLVDVRSPDEFSGRLLAPAHLP
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEECCCCCC
QEQSQRAGHVPTAKNIPWARTANEDGTFKSNADLEKLYSEAGVDLSKDTIAYCRIGERSA
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCC
HTWFALHELLGLANVKNYDGSWTEYGSLVGVPIEKGA
HHHHHHHHHHHHHCCCCCCCCCHHCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2294090 [H]