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The map label for this gene is hemL

Identifier: 158317865

GI number: 158317865

Start: 7450604

End: 7451953

Strand: Reverse

Name: hemL

Synonym: Franean1_6123

Alternate gene names: 158317865

Gene position: 7451953-7450604 (Counterclockwise)

Preceding gene: 158317870

Following gene: 158317864

Centisome position: 82.97

GC content: 74.59

Gene sequence:

>1350_bases
ATGGTGGTTCCGTCTACCGCACCCGCATCCGATGATCTCTTCCGGCGGGCCCAGCGGGTCGTCCCCGGCGGGGTGAACTC
GCCGGTGCGTGCCTTCCGCGCCGTCGGGGGCACTCCCCGCTTCATGACCTCCGGGTCCGGCCCGCGGCTCGTCGACGCCG
ACGGCCGCACCTACGTAGACCTTGTGTGCTCCTGGGGTCCGATGATCCTGGGCCACGCCCATCCCGACGTGGTCCAGGCG
ATCACGGCGGCCGCGGCCCGGGGCACCAGCTTCGGTACGCCCACCCCCGGCGAGGTGGAGCTCGCGGAGCTCATCGTCGA
GCGGGTGGCCCCGGTCGAGCAGGTGCGCCTGGTGAACTCCGGCACCGAGGCGACGATGAGCGCGATCCGGCTGGCCCGGG
GGTTCACCGGACGTCCCACGATCGTCAAGTTCGCGGGCTGCTACCACGGGCACGTCGACGCGCTGCTCGCGTCGGCCGGC
TCCGGGGTGGCGACCCTCGGCCTGCCCGACACCCCGGGCGTCACCGGCGCGGCGACCGCCGACACGATCGTCCTGCCCTA
CAACGATCTCGCCCTGGTCGAGGCGACCTTCGCCGAGCGCGGCGACACGATCGCGGCGGTCATCACCGAGTCCGTCGCGG
CGAACATGGGGGTCGTCCCGCCGCTGCCCGGTTTCAACGCCGGCCTGCGGCGCCTGTGCGACGCCCACGGCGCCCTGCTG
GTGTTCGACGAGGTGATGACGGGGTTCCGCGTCTCCCGCACCGGCTGGTGGGGCCTGGAGGGCGCCGTGGAGGGCTGGGC
GCCCGACCTGTTCACCTTCGGCAAGGTGATGGGCGGCGGCCTGCCGGCCGCGGCCTTCGGTGGCCGTGCGGACGTGATGG
CCCGACTGGCCCCCGCCGGCCCCGTCTACCAGGCCGGGACGCTCTCCGGGAACCCGCTCGCGGTGGCCGCCGGGCTCGCC
ACGCTGCGCGCCTGCACCGACGAGGTCTACGCGACGGTCGACGCGCGCGCCGCCGACGTCGCCGGCATCGTCTCCACCGC
CCTGAACGAGCAGGGCGTCCCGCACCTGCTCAACAGCGCTGGGTCGCTGTTCAGCGTGTTCTTCACCGACGCGACGGCGG
TCATCGACTACGCCGGCGCGAAGCGGCAGGCCAGCGAGCGTTACGCCGCCTTCTTCCACTCCATGCTCGACGCGGGGGTC
TACCTGCCGCCGTCGGCCTTCGAGGCGTGGTTCGTCTCGGCGGCGCACGACGACGAGGCGGTCGAGCGGATCGCCGCGGC
GGCCCCGGCGGCTGCCCGGGCCGCCGCGGCGGTGGGCGCGCCGGTCGCGGTCGGCGGGGGGGCCGTGTGA

Upstream 100 bases:

>100_bases
GTGCCGACCGCTCCGGCCCCGCTCTCGCTCTCGGTGCCGGATCCGCGGCAGCACCCGCGCCGGGCGCGGGCTCGCGGCGA
GACCTGCGAGGATCGGGGGC

Downstream 100 bases:

>100_bases
GCGGGCCGGAGGTCACGACCGTGCACCTCGTCCGGCACGGCGAGGTGTTCAACCCGAGCAAGGTGCTCTACGGCCGGCTG
CCCGGCTACCGGCTCTCCGA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MVVPSTAPASDDLFRRAQRVVPGGVNSPVRAFRAVGGTPRFMTSGSGPRLVDADGRTYVDLVCSWGPMILGHAHPDVVQA
ITAAAARGTSFGTPTPGEVELAELIVERVAPVEQVRLVNSGTEATMSAIRLARGFTGRPTIVKFAGCYHGHVDALLASAG
SGVATLGLPDTPGVTGAATADTIVLPYNDLALVEATFAERGDTIAAVITESVAANMGVVPPLPGFNAGLRRLCDAHGALL
VFDEVMTGFRVSRTGWWGLEGAVEGWAPDLFTFGKVMGGGLPAAAFGGRADVMARLAPAGPVYQAGTLSGNPLAVAAGLA
TLRACTDEVYATVDARAADVAGIVSTALNEQGVPHLLNSAGSLFSVFFTDATAVIDYAGAKRQASERYAAFFHSMLDAGV
YLPPSAFEAWFVSAAHDDEAVERIAAAAPAAARAAAAVGAPVAVGGGAV

Sequences:

>Translated_449_residues
MVVPSTAPASDDLFRRAQRVVPGGVNSPVRAFRAVGGTPRFMTSGSGPRLVDADGRTYVDLVCSWGPMILGHAHPDVVQA
ITAAAARGTSFGTPTPGEVELAELIVERVAPVEQVRLVNSGTEATMSAIRLARGFTGRPTIVKFAGCYHGHVDALLASAG
SGVATLGLPDTPGVTGAATADTIVLPYNDLALVEATFAERGDTIAAVITESVAANMGVVPPLPGFNAGLRRLCDAHGALL
VFDEVMTGFRVSRTGWWGLEGAVEGWAPDLFTFGKVMGGGLPAAAFGGRADVMARLAPAGPVYQAGTLSGNPLAVAAGLA
TLRACTDEVYATVDARAADVAGIVSTALNEQGVPHLLNSAGSLFSVFFTDATAVIDYAGAKRQASERYAAFFHSMLDAGV
YLPPSAFEAWFVSAAHDDEAVERIAAAAPAAARAAAAVGAPVAVGGGAV
>Mature_449_residues
MVVPSTAPASDDLFRRAQRVVPGGVNSPVRAFRAVGGTPRFMTSGSGPRLVDADGRTYVDLVCSWGPMILGHAHPDVVQA
ITAAAARGTSFGTPTPGEVELAELIVERVAPVEQVRLVNSGTEATMSAIRLARGFTGRPTIVKFAGCYHGHVDALLASAG
SGVATLGLPDTPGVTGAATADTIVLPYNDLALVEATFAERGDTIAAVITESVAANMGVVPPLPGFNAGLRRLCDAHGALL
VFDEVMTGFRVSRTGWWGLEGAVEGWAPDLFTFGKVMGGGLPAAAFGGRADVMARLAPAGPVYQAGTLSGNPLAVAAGLA
TLRACTDEVYATVDARAADVAGIVSTALNEQGVPHLLNSAGSLFSVFFTDATAVIDYAGAKRQASERYAAFFHSMLDAGV
YLPPSAFEAWFVSAAHDDEAVERIAAAAPAAARAAAAVGAPVAVGGGAV

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=352, Percent_Identity=30.9659090909091, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI24119277, Length=369, Percent_Identity=28.9972899728997, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI37574042, Length=323, Percent_Identity=27.5541795665635, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI226442709, Length=303, Percent_Identity=27.7227722772277, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI13994255, Length=324, Percent_Identity=27.4691358024691, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI226442705, Length=254, Percent_Identity=28.740157480315, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI284507298, Length=237, Percent_Identity=32.0675105485232, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI1786349, Length=420, Percent_Identity=54.047619047619, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1789016, Length=339, Percent_Identity=31.5634218289086, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1789759, Length=302, Percent_Identity=33.4437086092715, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1788044, Length=361, Percent_Identity=31.3019390581717, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI145693181, Length=352, Percent_Identity=29.2613636363636, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787560, Length=402, Percent_Identity=29.8507462686567, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71992977, Length=317, Percent_Identity=27.7602523659306, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI25144271, Length=302, Percent_Identity=26.8211920529801, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144274, Length=145, Percent_Identity=31.7241379310345, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324432, Length=318, Percent_Identity=27.0440251572327, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323470, Length=304, Percent_Identity=26.6447368421053, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI28574759, Length=326, Percent_Identity=30.6748466257669, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI161085790, Length=326, Percent_Identity=30.6748466257669, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI21356575, Length=309, Percent_Identity=27.1844660194175, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21357415, Length=316, Percent_Identity=25.3164556962025, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_FRASN (A8LCD0)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001510373.1
- ProteinModelPortal:   A8LCD0
- GeneID:   5674444
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_6123
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- ProtClustDB:   PRK00062
- BioCyc:   FSP1855:FRANEAN1_6123-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45740; Mature: 45740

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVPSTAPASDDLFRRAQRVVPGGVNSPVRAFRAVGGTPRFMTSGSGPRLVDADGRTYVD
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEECCCCCEEECCCCCEEEE
LVCSWGPMILGHAHPDVVQAITAAAARGTSFGTPTPGEVELAELIVERVAPVEQVRLVNS
EEECCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCC
GTEATMSAIRLARGFTGRPTIVKFAGCYHGHVDALLASAGSGVATLGLPDTPGVTGAATA
CHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
DTIVLPYNDLALVEATFAERGDTIAAVITESVAANMGVVPPLPGFNAGLRRLCDAHGALL
CEEEECCCCHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEE
VFDEVMTGFRVSRTGWWGLEGAVEGWAPDLFTFGKVMGGGLPAAAFGGRADVMARLAPAG
EEHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCHHHHHHHHCCCC
PVYQAGTLSGNPLAVAAGLATLRACTDEVYATVDARAADVAGIVSTALNEQGVPHLLNSA
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHCC
GSLFSVFFTDATAVIDYAGAKRQASERYAAFFHSMLDAGVYLPPSAFEAWFVSAAHDDEA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEEECCCCHHH
VERIAAAAPAAARAAAAVGAPVAVGGGAV
HHHHHHHCCHHHHHHHHHCCCEECCCCCC
>Mature Secondary Structure
MVVPSTAPASDDLFRRAQRVVPGGVNSPVRAFRAVGGTPRFMTSGSGPRLVDADGRTYVD
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEECCCCCEEECCCCCEEEE
LVCSWGPMILGHAHPDVVQAITAAAARGTSFGTPTPGEVELAELIVERVAPVEQVRLVNS
EEECCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCC
GTEATMSAIRLARGFTGRPTIVKFAGCYHGHVDALLASAGSGVATLGLPDTPGVTGAATA
CHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
DTIVLPYNDLALVEATFAERGDTIAAVITESVAANMGVVPPLPGFNAGLRRLCDAHGALL
CEEEECCCCHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEE
VFDEVMTGFRVSRTGWWGLEGAVEGWAPDLFTFGKVMGGGLPAAAFGGRADVMARLAPAG
EEHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCHHHHHHHHCCCC
PVYQAGTLSGNPLAVAAGLATLRACTDEVYATVDARAADVAGIVSTALNEQGVPHLLNSA
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHCC
GSLFSVFFTDATAVIDYAGAKRQASERYAAFFHSMLDAGVYLPPSAFEAWFVSAAHDDEA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEEECCCCHHH
VERIAAAAPAAARAAAAVGAPVAVGGGAV
HHHHHHHCCHHHHHHHHHCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA