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The map label for this gene is cofH [H]

Identifier: 158317853

GI number: 158317853

Start: 7438201

End: 7439379

Strand: Reverse

Name: cofH [H]

Synonym: Franean1_6111

Alternate gene names: 158317853

Gene position: 7439379-7438201 (Counterclockwise)

Preceding gene: 158317854

Following gene: 158317852

Centisome position: 82.83

GC content: 67.35

Gene sequence:

>1179_bases
TTGTCGGTCCGCGGAGCTAGGCTGGATCGCATGGATGCCGGGCTCAAGCGCGAGATCGAAGCCAAGGTCCACGACGGTGC
CCGGCTCAGCCGGGCCGACGGTGAGGCCCTCTACGCGAGCGACGACCTCGCCTGGCTGGGCGGTCTCGCGCACGAGGTGC
GTACCCGAAAGAACGGCGACCAGACCTTCTTCAACGTGAACCGGCACCTCAACCTGACGAACGTCTGCTCGGCCTCGTGC
GCCTACTGCTCGTTCCAACGCAAGCCCGGCGAGTCGGACGCCTACACTATGCGCATCGAGGAGGCCGTCCGGCTGGCGAA
GGATATGGAGCCGGCCGGGATCACCGAGCTGCACATCGTCAACGGCCTGCACCCGACGTTGCCGTGGCGTTACTATCCGC
GGTCGCTGCGCGAGCTGGGGAAGGCGCTGCCCGGCGTCGCGCTCAAGGCGTTCACCGCCACCGAGATCCACTGGTTCGAG
AAGATCAGCGGCCTCTCCGCGGACGAGATCCTGGACGAGCTCATCGACGCGGGCCTGGAGTCGTTGACGGGCGGCGGCGC
GGAGATCTTCGACTGGGAGGTGCGGCAGAAGATCGTCGGCCACGAGACCCACTGGGAGGACTGGTCGCGGATCCACCGTC
TCGCGCACTCCAAGGGCCTGCGCACGCCGTGCACGATGCTGTACGGCCATGTCGAGGAGCCGCGGCACCGGGTCGACCAC
GTGCTGCGCCTGCGTGAGCTGCAGGACGAGACGGGCGGTTTCGCGGTGTTCATCCCGCTGCGCTTCCAGCACGACTCGGT
CGGCGATCCCCGCAACCGCCTGATGAACCAGCCGATGGCGACCGGCGCGGAGGCTCTCAAGACGTTCGCGGTGTCGCGGC
TGCTGTTCGACAACGTCGATCACGTCAAGTGCTTCTGGGTGATGCACGGGCTGACCACCGCCCAGCTGTCCCTGAACTTC
GGCGTCGACGACCTCGACGGCTCGGTCGTCGAGTACAAGATCACTCACGACGCGGACGGCTTCGGAACGCCGAACACGAT
GACCCGGGAGGATCTTCTATCCGTGATCCGTGACGCGGGCTTCCGGCCGGTCGAGCGGGACACCCGCTACCGGGTCGTGC
GCAGGTACGACGGTCCGGACACCACCCGGCGGGACAACCCCGTCTCGATCGACGCCTGA

Upstream 100 bases:

>100_bases
CGCCCGCTTCCCGAGGGTGACTGCGCATGGTCCGGGCAGCGGCCGCGGTCGGGCCTGCGGCGGGGATCACGGCACCGGTG
GCGGACCGGGACGTCATGGA

Downstream 100 bases:

>100_bases
CCCGGCGATGGCGCTCGAGGAGAACCCGGCCCGGTCGGGCGGCGGATCGCGGCCCGACAGCCCGGTCGTGGCCGGCGGGC
CGGCGACGCCCGGCCCGGCG

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSVRGARLDRMDAGLKREIEAKVHDGARLSRADGEALYASDDLAWLGGLAHEVRTRKNGDQTFFNVNRHLNLTNVCSASC
AYCSFQRKPGESDAYTMRIEEAVRLAKDMEPAGITELHIVNGLHPTLPWRYYPRSLRELGKALPGVALKAFTATEIHWFE
KISGLSADEILDELIDAGLESLTGGGAEIFDWEVRQKIVGHETHWEDWSRIHRLAHSKGLRTPCTMLYGHVEEPRHRVDH
VLRLRELQDETGGFAVFIPLRFQHDSVGDPRNRLMNQPMATGAEALKTFAVSRLLFDNVDHVKCFWVMHGLTTAQLSLNF
GVDDLDGSVVEYKITHDADGFGTPNTMTREDLLSVIRDAGFRPVERDTRYRVVRRYDGPDTTRRDNPVSIDA

Sequences:

>Translated_392_residues
MSVRGARLDRMDAGLKREIEAKVHDGARLSRADGEALYASDDLAWLGGLAHEVRTRKNGDQTFFNVNRHLNLTNVCSASC
AYCSFQRKPGESDAYTMRIEEAVRLAKDMEPAGITELHIVNGLHPTLPWRYYPRSLRELGKALPGVALKAFTATEIHWFE
KISGLSADEILDELIDAGLESLTGGGAEIFDWEVRQKIVGHETHWEDWSRIHRLAHSKGLRTPCTMLYGHVEEPRHRVDH
VLRLRELQDETGGFAVFIPLRFQHDSVGDPRNRLMNQPMATGAEALKTFAVSRLLFDNVDHVKCFWVMHGLTTAQLSLNF
GVDDLDGSVVEYKITHDADGFGTPNTMTREDLLSVIRDAGFRPVERDTRYRVVRRYDGPDTTRRDNPVSIDA
>Mature_391_residues
SVRGARLDRMDAGLKREIEAKVHDGARLSRADGEALYASDDLAWLGGLAHEVRTRKNGDQTFFNVNRHLNLTNVCSASCA
YCSFQRKPGESDAYTMRIEEAVRLAKDMEPAGITELHIVNGLHPTLPWRYYPRSLRELGKALPGVALKAFTATEIHWFEK
ISGLSADEILDELIDAGLESLTGGGAEIFDWEVRQKIVGHETHWEDWSRIHRLAHSKGLRTPCTMLYGHVEEPRHRVDHV
LRLRELQDETGGFAVFIPLRFQHDSVGDPRNRLMNQPMATGAEALKTFAVSRLLFDNVDHVKCFWVMHGLTTAQLSLNFG
VDDLDGSVVEYKITHDADGFGTPNTMTREDLLSVIRDAGFRPVERDTRYRVVRRYDGPDTTRRDNPVSIDA

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 44274; Mature: 44143

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRGARLDRMDAGLKREIEAKVHDGARLSRADGEALYASDDLAWLGGLAHEVRTRKNGD
CCCCCCCHHHHHCCHHHHHHHHHCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCC
QTFFNVNRHLNLTNVCSASCAYCSFQRKPGESDAYTMRIEEAVRLAKDMEPAGITELHIV
CEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCEEEEEE
NGLHPTLPWRYYPRSLRELGKALPGVALKAFTATEIHWFEKISGLSADEILDELIDAGLE
CCCCCCCCCHHCCHHHHHHHHHCCCHHEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SLTGGGAEIFDWEVRQKIVGHETHWEDWSRIHRLAHSKGLRTPCTMLYGHVEEPRHRVDH
HHCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHH
VLRLRELQDETGGFAVFIPLRFQHDSVGDPRNRLMNQPMATGAEALKTFAVSRLLFDNVD
HHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
HVKCFWVMHGLTTAQLSLNFGVDDLDGSVVEYKITHDADGFGTPNTMTREDLLSVIRDAG
CEEEEEHHHCCEEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCC
FRPVERDTRYRVVRRYDGPDTTRRDNPVSIDA
CCCCCCCHHEEHEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVRGARLDRMDAGLKREIEAKVHDGARLSRADGEALYASDDLAWLGGLAHEVRTRKNGD
CCCCCCHHHHHCCHHHHHHHHHCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCC
QTFFNVNRHLNLTNVCSASCAYCSFQRKPGESDAYTMRIEEAVRLAKDMEPAGITELHIV
CEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCEEEEEE
NGLHPTLPWRYYPRSLRELGKALPGVALKAFTATEIHWFEKISGLSADEILDELIDAGLE
CCCCCCCCCHHCCHHHHHHHHHCCCHHEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SLTGGGAEIFDWEVRQKIVGHETHWEDWSRIHRLAHSKGLRTPCTMLYGHVEEPRHRVDH
HHCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHH
VLRLRELQDETGGFAVFIPLRFQHDSVGDPRNRLMNQPMATGAEALKTFAVSRLLFDNVD
HHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
HVKCFWVMHGLTTAQLSLNFGVDDLDGSVVEYKITHDADGFGTPNTMTREDLLSVIRDAG
CEEEEEHHHCCEEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCC
FRPVERDTRYRVVRRYDGPDTTRRDNPVSIDA
CCCCCCCHHEEHEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]