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The map label for this gene is nuoI

Identifier: 158317828

GI number: 158317828

Start: 7408642

End: 7409277

Strand: Reverse

Name: nuoI

Synonym: Franean1_6086

Alternate gene names: 158317828

Gene position: 7409277-7408642 (Counterclockwise)

Preceding gene: 158317829

Following gene: 158317827

Centisome position: 82.49

GC content: 69.81

Gene sequence:

>636_bases
ATGGGGATCCTCGACCCCTACAAGGGTTTCGGCGTCACCTTCTCGACGATGTTCAAGAAGCCGACGACCGAGCAGTACCC
GGAGCAGAAGAAGGAGACGGCACCCCGCTTCCACGGCCGTCACCAGCTCAACCGGCATCCGGACGGGCTGGAGAAGTGCG
TCGGCTGCGAGCTGTGCGCGTGGGCCTGCCCGGCCGACGCGATCTACGTCGAGGGCGCGGACAACACCGACGAGCAGCGT
TTCTCGCCGGGTGAGCGGTACGGCCGCGTCTACCAGATCAACTACCTGCGCTGCATCCTGTGCGGGCTGTGCATCGAGGC
GTGCCCCACCCGGGCGCTGACGATGTCGAACGACTACGAGCTGGCCGACGACAGCCGCGACGACCTGATCTTCACCAAGG
AGCAGCTGCTCGCCCCGCTTCGGGCCGGGATGGAGTCCCCGCCGCACCCGATGCGCCTCGGCGAGAGCGAGACCGACTAC
TACACCCGCGATCCCGACGCCCCGCTGCCCTGGCAGGTCGGCGGCTCGCCGGCCGACGAAGCTGACGAGGCCGGCGAGGC
CGGCGAGGCCGGCGAGGCCGAAAGGGCGGCGGACAAGGTGCCCGCCCATGGCGCGGGTTCGGAGCGGCCCCGATGA

Upstream 100 bases:

>100_bases
CACTGCAGCCGGCAGCGGCGCGGCCGGCTCCGCCGGCGGCGACAAGGGAAACACCACCGTCATCCCCGCGGGCTCCGGCC
CGCGACAGGAGAGCTGAACC

Downstream 100 bases:

>100_bases
ATCCGCAGATTCTCGCCCAGGCGGCGGAGATCAGCAGCACGTCGAACGGCGAGGCCTGGACGTTCTGGCTGCTCGCGCCG
GTGGCGCTGCTGGCGGCGCT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MGILDPYKGFGVTFSTMFKKPTTEQYPEQKKETAPRFHGRHQLNRHPDGLEKCVGCELCAWACPADAIYVEGADNTDEQR
FSPGERYGRVYQINYLRCILCGLCIEACPTRALTMSNDYELADDSRDDLIFTKEQLLAPLRAGMESPPHPMRLGESETDY
YTRDPDAPLPWQVGGSPADEADEAGEAGEAGEAERAADKVPAHGAGSERPR

Sequences:

>Translated_211_residues
MGILDPYKGFGVTFSTMFKKPTTEQYPEQKKETAPRFHGRHQLNRHPDGLEKCVGCELCAWACPADAIYVEGADNTDEQR
FSPGERYGRVYQINYLRCILCGLCIEACPTRALTMSNDYELADDSRDDLIFTKEQLLAPLRAGMESPPHPMRLGESETDY
YTRDPDAPLPWQVGGSPADEADEAGEAGEAGEAERAADKVPAHGAGSERPR
>Mature_210_residues
GILDPYKGFGVTFSTMFKKPTTEQYPEQKKETAPRFHGRHQLNRHPDGLEKCVGCELCAWACPADAIYVEGADNTDEQRF
SPGERYGRVYQINYLRCILCGLCIEACPTRALTMSNDYELADDSRDDLIFTKEQLLAPLRAGMESPPHPMRLGESETDYY
TRDPDAPLPWQVGGSPADEADEAGEAGEAGEAERAADKVPAHGAGSERPR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=131, Percent_Identity=38.9312977099237, Blast_Score=113, Evalue=1e-25,
Organism=Escherichia coli, GI1788617, Length=144, Percent_Identity=40.2777777777778, Blast_Score=111, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17555194, Length=131, Percent_Identity=38.9312977099237, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17864306, Length=130, Percent_Identity=40, Blast_Score=114, Evalue=4e-26,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_FRASN (A8LC93)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001510336.1
- ProteinModelPortal:   A8LC93
- SMR:   A8LC93
- GeneID:   5674407
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_6086
- HOGENOM:   HBG724359
- OMA:   DAPLPWQ
- ProtClustDB:   PRK05888
- BioCyc:   FSP1855:FRANEAN1_6086-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 23359; Mature: 23228

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGILDPYKGFGVTFSTMFKKPTTEQYPEQKKETAPRFHGRHQLNRHPDGLEKCVGCELCA
CCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCHHCCCHHCCCCCCHHHHHHCCHHHH
WACPADAIYVEGADNTDEQRFSPGERYGRVYQINYLRCILCGLCIEACPTRALTMSNDYE
HCCCCCEEEEECCCCCCHHHCCCHHHCCCEEEEHHHHHHHHHHHHHHCCCCEEECCCCCC
LADDSRDDLIFTKEQLLAPLRAGMESPPHPMRLGESETDYYTRDPDAPLPWQVGGSPADE
CCCCCCCCEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCH
ADEAGEAGEAGEAERAADKVPAHGAGSERPR
HHHCCCCCCCCCHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
GILDPYKGFGVTFSTMFKKPTTEQYPEQKKETAPRFHGRHQLNRHPDGLEKCVGCELCA
CCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCHHCCCHHCCCCCCHHHHHHCCHHHH
WACPADAIYVEGADNTDEQRFSPGERYGRVYQINYLRCILCGLCIEACPTRALTMSNDYE
HCCCCCEEEEECCCCCCHHHCCCHHHCCCEEEEHHHHHHHHHHHHHHCCCCEEECCCCCC
LADDSRDDLIFTKEQLLAPLRAGMESPPHPMRLGESETDYYTRDPDAPLPWQVGGSPADE
CCCCCCCCEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCH
ADEAGEAGEAGEAERAADKVPAHGAGSERPR
HHHCCCCCCCCCHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA