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The map label for this gene is pyrE [H]

Identifier: 158317813

GI number: 158317813

Start: 7389341

End: 7389868

Strand: Reverse

Name: pyrE [H]

Synonym: Franean1_6071

Alternate gene names: 158317813

Gene position: 7389868-7389341 (Counterclockwise)

Preceding gene: 158317814

Following gene: 158317812

Centisome position: 82.27

GC content: 72.16

Gene sequence:

>528_bases
GTGGCCGACATCGAAGCGCTGGCCGCCCGGGTGCGGGCGGCGTCTCACCTGACCGGGACGTTCCTCCTCCGGTCGGGCCG
TACGAGCACCGAGTACTTCGACAAGTACCTGTTCGAGGCCGAGCCGGCGCTGCTCGCCGACATCGTCGAGGCGATGGCGG
CGCATCTCCCGCCGAACACCGATCTCCTCGCCGGCCTGGAGCTGGGCGGCGTTCCGCTGGCCACGCTGCTGTCGGCACGC
ACCGGCCTGCCGGCCAGGTTCGTCCGCAAGAAGGCGAAGGAGTACGGAACCCGGCGCATAGCCGAGGGCGGCGAGGTCGA
GGGTCGGCGGATCGTCCTGGTCGAGGACGTCGTGACCAGCGGCGGGGCCGTGCTCGACGCCACCCGGGTGCTGCGCGAGC
AGGGCGCGGTCGTCGAGGACGTCCTGTGCGTCATCGATCGCCAGGAGGGTGGCCGGGAGCGGCTCGCGGAGATCGGCCTG
ACGCTGCTCCCCACTCTCACCCGCGCCGATCTCGACGCGGCTCGCTAG

Upstream 100 bases:

>100_bases
CTACACCGGCGCCTACTGCCGCACCGCTGCCCGCCGCATCGCCTCCATCGTCGGCCGAAGCCTGCTGGCGGCGCAGTGGC
GGGCCGTAGAGTCGGCAGAC

Downstream 100 bases:

>100_bases
CACCCGGACAGCCTGACGTCGGACACGCGGGACCGGGATGACTTCGGACGTGGCCGGAGTGTTGTTCGTCTATGGGACGC
TGCGCTTCGACGTGGTCGTC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MADIEALAARVRAASHLTGTFLLRSGRTSTEYFDKYLFEAEPALLADIVEAMAAHLPPNTDLLAGLELGGVPLATLLSAR
TGLPARFVRKKAKEYGTRRIAEGGEVEGRRIVLVEDVVTSGGAVLDATRVLREQGAVVEDVLCVIDRQEGGRERLAEIGL
TLLPTLTRADLDAAR

Sequences:

>Translated_175_residues
MADIEALAARVRAASHLTGTFLLRSGRTSTEYFDKYLFEAEPALLADIVEAMAAHLPPNTDLLAGLELGGVPLATLLSAR
TGLPARFVRKKAKEYGTRRIAEGGEVEGRRIVLVEDVVTSGGAVLDATRVLREQGAVVEDVLCVIDRQEGGRERLAEIGL
TLLPTLTRADLDAAR
>Mature_174_residues
ADIEALAARVRAASHLTGTFLLRSGRTSTEYFDKYLFEAEPALLADIVEAMAAHLPPNTDLLAGLELGGVPLATLLSART
GLPARFVRKKAKEYGTRRIAEGGEVEGRRIVLVEDVVTSGGAVLDATRVLREQGAVVEDVLCVIDRQEGGRERLAEIGLT
LLPTLTRADLDAAR

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=156, Percent_Identity=34.6153846153846, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1790073, Length=152, Percent_Identity=34.2105263157895, Blast_Score=71, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=158, Percent_Identity=37.3417721518987, Blast_Score=102, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17508631, Length=153, Percent_Identity=35.9477124183007, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323927, Length=144, Percent_Identity=31.9444444444444, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323530, Length=144, Percent_Identity=30.5555555555556, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17933654, Length=152, Percent_Identity=33.5526315789474, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 18778; Mature: 18647

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIEALAARVRAASHLTGTFLLRSGRTSTEYFDKYLFEAEPALLADIVEAMAAHLPPNT
CCCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
DLLAGLELGGVPLATLLSARTGLPARFVRKKAKEYGTRRIAEGGEVEGRRIVLVEDVVTS
CCEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHC
GGAVLDATRVLREQGAVVEDVLCVIDRQEGGRERLAEIGLTLLPTLTRADLDAAR
CCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ADIEALAARVRAASHLTGTFLLRSGRTSTEYFDKYLFEAEPALLADIVEAMAAHLPPNT
CCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
DLLAGLELGGVPLATLLSARTGLPARFVRKKAKEYGTRRIAEGGEVEGRRIVLVEDVVTS
CCEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHC
GGAVLDATRVLREQGAVVEDVLCVIDRQEGGRERLAEIGLTLLPTLTRADLDAAR
CCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA