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The map label for this gene is tuf [H]

Identifier: 158317793

GI number: 158317793

Start: 7362625

End: 7363818

Strand: Reverse

Name: tuf [H]

Synonym: Franean1_6051

Alternate gene names: 158317793

Gene position: 7363818-7362625 (Counterclockwise)

Preceding gene: 158317794

Following gene: 158317792

Centisome position: 81.98

GC content: 65.91

Gene sequence:

>1194_bases
GTGGCGAAGCAGAAGTTCGAGCGGACCAAGCCGCACGTCAACATCGGGACCATCGGTCACATCGACCATGGCAAGACGAC
GCTGACGGCCGCGATCACGAAGGTCCTGCACGACGCGCACCCGGACCTCAACCCGTTCACGCCGTTCGACCAGATCGACA
AGGCGCCGGAGGAGAAGGCCCGCGGGATCACGATCTCGATCGCGCACGTCGAGTACCAGACCGACGCGCGGCACTACGCG
CACGTCGACTGCCCCGGGCACGCCGACTACATCAAGAACATGATCACGGGTGCCGCGCAGATGGACGGCGCGATCCTGGT
GGTCTCGGCGACCGACGGCCCCATGCCGCAGACGAAGGAGCACGTGCTCCTGGCCCGCCAGGTCGGCGTGCCGTACATCG
TCGTGGCGCTGAACAAGGCCGACATGGTCGACGACGAGGAGATCCTCGAGCTCGTCGAGCTCGAGGTCCGGGAGCTCCTG
AACACCTACGAGTTCCCCGGCGACGACGTCCCGGTCATCCGCGTCTCCGCGCTCAAGGCGCTGGAGGGCGACAAGGAGTG
GGGCGAGAAGCTCCTCGAGCTCATGGCGGCGGTGGACGCGTCCATCCCCGAGCCTGAGCGTGACATCGACCGGCCGTTCC
TCATGCCGATCGAGGACGTGTTCACGATCACGGGCCGTGGCACGGTCGTCACGGGTCGTATCGAGCGTGGAATCGTCAAG
GTCAACGAGACCGTGGAGATCGTCGGCATCAAGCCGGAGACCACCACGACGACCGTGACCGGCGTCGAGATGTTCCGCAA
GCTGCTCGACGAGGGCCAGGCCGGTGACAACGTCGGCCTCCTCCTCCGTGGCATCAAGCGCGAGGACGTCGAGCGCGGCC
AGGTCATCGTGAAGCCGAAGTCGATCACCCCCCACACGGTGTTCGAGGCGCGCGTCTACATCCTCAACAAGGACGAGGGT
GGCCGGCACACGCCGTTCTTCAAGAACTACCGGCCGCAGTTCTACTTCCGCACCACCGACGTGACCGGCGTCGTGACCCT
CCCCGAGGGCACCGAGATGGTCATGCCGGGCGACAACACCGAGATGACGGTCGAGCTCATCCAGCCGATCGCCATGGAGG
AGGGCCTGCGGTTCGCCATCCGTGAGGGTGGCCGGACCGTCGGTGCGGGTCAGGTTCTGAAGGTCATCAAGTAG

Upstream 100 bases:

>100_bases
ATCGCGAAGGCGAGGGGCGAGTAGCAGGCCCGCCGGGCCGGGCTGCCGGCACCACTGACGACAACACAGCACGACACCAC
CCGTCCTTGGAGGGACACCA

Downstream 100 bases:

>100_bases
TGGCGGCGGGCGGCGGGTGCCCCACGGGGGCCCGCCGCCCGCTGTTGCTGGCCGGCCACGCCGGGGCGTAACGCTCCGCG
TCGCGACCAGGATCGGAAGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAKQKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLHDAHPDLNPFTPFDQIDKAPEEKARGITISIAHVEYQTDARHYA
HVDCPGHADYIKNMITGAAQMDGAILVVSATDGPMPQTKEHVLLARQVGVPYIVVALNKADMVDDEEILELVELEVRELL
NTYEFPGDDVPVIRVSALKALEGDKEWGEKLLELMAAVDASIPEPERDIDRPFLMPIEDVFTITGRGTVVTGRIERGIVK
VNETVEIVGIKPETTTTTVTGVEMFRKLLDEGQAGDNVGLLLRGIKREDVERGQVIVKPKSITPHTVFEARVYILNKDEG
GRHTPFFKNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTEMTVELIQPIAMEEGLRFAIREGGRTVGAGQVLKVIK

Sequences:

>Translated_397_residues
MAKQKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLHDAHPDLNPFTPFDQIDKAPEEKARGITISIAHVEYQTDARHYA
HVDCPGHADYIKNMITGAAQMDGAILVVSATDGPMPQTKEHVLLARQVGVPYIVVALNKADMVDDEEILELVELEVRELL
NTYEFPGDDVPVIRVSALKALEGDKEWGEKLLELMAAVDASIPEPERDIDRPFLMPIEDVFTITGRGTVVTGRIERGIVK
VNETVEIVGIKPETTTTTVTGVEMFRKLLDEGQAGDNVGLLLRGIKREDVERGQVIVKPKSITPHTVFEARVYILNKDEG
GRHTPFFKNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTEMTVELIQPIAMEEGLRFAIREGGRTVGAGQVLKVIK
>Mature_396_residues
AKQKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLHDAHPDLNPFTPFDQIDKAPEEKARGITISIAHVEYQTDARHYAH
VDCPGHADYIKNMITGAAQMDGAILVVSATDGPMPQTKEHVLLARQVGVPYIVVALNKADMVDDEEILELVELEVRELLN
TYEFPGDDVPVIRVSALKALEGDKEWGEKLLELMAAVDASIPEPERDIDRPFLMPIEDVFTITGRGTVVTGRIERGIVKV
NETVEIVGIKPETTTTTVTGVEMFRKLLDEGQAGDNVGLLLRGIKREDVERGQVIVKPKSITPHTVFEARVYILNKDEGG
RHTPFFKNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTEMTVELIQPIAMEEGLRFAIREGGRTVGAGQVLKVIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=397, Percent_Identity=56.1712846347607, Blast_Score=450, Evalue=1e-127,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=29.5454545454545, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=29.5454545454545, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI4503471, Length=451, Percent_Identity=29.490022172949, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4503475, Length=355, Percent_Identity=32.112676056338, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI54607086, Length=335, Percent_Identity=28.6567164179104, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI194018522, Length=437, Percent_Identity=25.858123569794, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI194018520, Length=437, Percent_Identity=25.858123569794, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI194097354, Length=437, Percent_Identity=25.858123569794, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI46094014, Length=447, Percent_Identity=25.2796420581655, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI4503507, Length=289, Percent_Identity=26.2975778546713, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI82546879, Length=437, Percent_Identity=24.0274599542334, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI53729339, Length=226, Percent_Identity=31.858407079646, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI53729337, Length=226, Percent_Identity=31.858407079646, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI45593140, Length=428, Percent_Identity=21.9626168224299, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790412, Length=397, Percent_Identity=76.0705289672544, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=76.2626262626263, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI2367247, Length=338, Percent_Identity=32.5443786982249, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789108, Length=335, Percent_Identity=29.5522388059701, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI48994988, Length=282, Percent_Identity=32.9787234042553, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17556456, Length=398, Percent_Identity=50.7537688442211, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25141371, Length=375, Percent_Identity=46.4, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=449, Percent_Identity=30.5122494432071, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=449, Percent_Identity=30.5122494432071, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=29.4930875576037, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=442, Percent_Identity=29.6380090497738, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI115532065, Length=439, Percent_Identity=29.6127562642369, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32566629, Length=455, Percent_Identity=26.1538461538462, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=275, Percent_Identity=29.0909090909091, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=36.734693877551, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=296, Percent_Identity=26.0135135135135, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=238, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17565438, Length=434, Percent_Identity=24.1935483870968, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=62.8140703517588, Blast_Score=500, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6325337, Length=350, Percent_Identity=32.5714285714286, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=350, Percent_Identity=32.5714285714286, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=353, Percent_Identity=25.2124645892351, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=343, Percent_Identity=28.8629737609329, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323320, Length=240, Percent_Identity=28.75, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320863, Length=289, Percent_Identity=25.6055363321799, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI281363316, Length=397, Percent_Identity=57.1788413098237, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI17864358, Length=397, Percent_Identity=57.1788413098237, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI19921738, Length=390, Percent_Identity=50.5128205128205, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=29.1111111111111, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=29.1111111111111, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=29.1111111111111, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=29.1111111111111, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24652838, Length=450, Percent_Identity=28.8888888888889, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI17137572, Length=450, Percent_Identity=28.8888888888889, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI45550900, Length=450, Percent_Identity=28.8888888888889, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=31.3725490196078, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI17137380, Length=339, Percent_Identity=28.9085545722714, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24647048, Length=305, Percent_Identity=24.9180327868852, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24647046, Length=305, Percent_Identity=24.9180327868852, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28572034, Length=166, Percent_Identity=36.144578313253, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43959; Mature: 43828

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLHDAHPDLNPFTPFDQIDKAPEEKA
CCCCHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHH
RGITISIAHVEYQTDARHYAHVDCPGHADYIKNMITGAAQMDGAILVVSATDGPMPQTKE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHH
HVLLARQVGVPYIVVALNKADMVDDEEILELVELEVRELLNTYEFPGDDVPVIRVSALKA
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
LEGDKEWGEKLLELMAAVDASIPEPERDIDRPFLMPIEDVFTITGRGTVVTGRIERGIVK
HCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHEEEEECCCEEEEEECCCCEEE
VNETVEIVGIKPETTTTTVTGVEMFRKLLDEGQAGDNVGLLLRGIKREDVERGQVIVKPK
ECCEEEEEEECCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHEECCCHHCCCCCEEEEECC
SITPHTVFEARVYILNKDEGGRHTPFFKNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNT
CCCCCEEEEEEEEEEECCCCCCCCCCHHCCCCEEEEEECCCEEEEECCCCCEEEECCCCC
EMTVELIQPIAMEEGLRFAIREGGRTVGAGQVLKVIK
EEHHHHHHHHHHHCCCEEEECCCCCEECHHHHHHHCC
>Mature Secondary Structure 
AKQKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLHDAHPDLNPFTPFDQIDKAPEEKA
CCCHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHH
RGITISIAHVEYQTDARHYAHVDCPGHADYIKNMITGAAQMDGAILVVSATDGPMPQTKE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHH
HVLLARQVGVPYIVVALNKADMVDDEEILELVELEVRELLNTYEFPGDDVPVIRVSALKA
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
LEGDKEWGEKLLELMAAVDASIPEPERDIDRPFLMPIEDVFTITGRGTVVTGRIERGIVK
HCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHEEEEECCCEEEEEECCCCEEE
VNETVEIVGIKPETTTTTVTGVEMFRKLLDEGQAGDNVGLLLRGIKREDVERGQVIVKPK
ECCEEEEEEECCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHEECCCHHCCCCCEEEEECC
SITPHTVFEARVYILNKDEGGRHTPFFKNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNT
CCCCCEEEEEEEEEEECCCCCCCCCCHHCCCCEEEEEECCCEEEEECCCCCEEEECCCCC
EMTVELIQPIAMEEGLRFAIREGGRTVGAGQVLKVIK
EEHHHHHHHHHHHCCCEEEECCCCCEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA