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The map label for this gene is rpoA [H]

Identifier: 158317764

GI number: 158317764

Start: 7344818

End: 7345945

Strand: Reverse

Name: rpoA [H]

Synonym: Franean1_6022

Alternate gene names: 158317764

Gene position: 7345945-7344818 (Counterclockwise)

Preceding gene: 158317765

Following gene: 158317763

Centisome position: 81.78

GC content: 67.29

Gene sequence:

>1128_bases
ATGCCCCCGTGCCGGCTCCTCTTCAGGGGCTTCCACGGCGTCATATGGCGGTCGCCGTGTTCGGAAGGAAGTTCTCTCAT
GCTGATCGCTCAGCGTCCCTCGCTCGCCGAGGACCCGATCTCCGAGTTCCGGTCGCGCTTCGTGATCGAGCCGCTCGAGC
CGGGCTTCGGCTACACCCTCGGCAACTCGCTGCGCCGCACCCTGCTGTCCTCCATCCCGGGCGCGGCCGTGACGAGTATC
CGGGTGGACGGCGTCCTCCACGAGTTCTCCACCGTTCCCGGGGTCAAGGAGGACGTGACCGACCTGATCCTGAACCTCAA
GGAACTGGTCGTCAGCTCCGACAACGACGAGCCGACCGTGATGTACCTGCGCAAGCAGGGCCCCGGTGAGGTCACCGCGG
CCGACATCGCCCCCCCGGCCGGCGTCGAGGTGCACAACCCCGACCTGCACCTGGCCACCCTCAACGACAAGGGCAAGCTC
GAGATCGAGCTGACCGTCGAGCGGGGCCGTGGCTATGTCAGCGCCGCCCAGAACAAGCAGCCGGGCCAGGAGATCGGTCG
CATTCCGATCGACTCCATCTACTCCCCGGTGCTGAAGGTCACCTACAAGGTCGAGGCGACCCGTGTGGAGCAGCGCACGG
ACTTCGACCGGCTCATCGTCGACGTGGAGACGAAGCAGTCGATCTCCCCACGGGACGCGATGGCCAGCGCCGGCAAGACC
CTCGTCGGCCTGTTCGGGCTGGCCCAGGAGCTCAACGCCGAGGCGGAGGGCGTCGACATCGGCCCGTCCGCGGCGGACGC
TGCCCTGGCCGCCGACCTGGCGCTGCCGATCGAGGAGATGGACCTGACCGTCCGCTCGTACAACTGCCTCAAGCGCGAGG
GCATCCACACCATCGGTGAGCTGGTGTCCCGCAGCGAGGCGGACCTGCTCGACATCCGCAACTTCGGGCAGAAGTCGATC
GACGAGGTCAAGACCAAGCTGGGTGCCATGGGCCTGCAGCTCAAGGACTCCCCGCCCGGGTTCGACCCGCGCCAGGCCGT
CGACACGTACGGCACCGACACGTACAACCCGTCGTTCTCCGACCCGTCCGATGACGGTCGCGAGTTCGTCGAGACCGAAC
AGTACTGA

Upstream 100 bases:

>100_bases
GCCCGGGGCCTGGGCATGGTGGCGGCACACGGGGATCCAAGGGGATCACGGTGGGCCGCGGAGGCCCAGGCGTACTGTTG
TGTGGTGCGGGGGCGGACCG

Downstream 100 bases:

>100_bases
TCTGACCGTGTCCCCGGGCGCCCGGTTAGCCGGGCGCCGGGGCACACGTCCACCGGGGGCCTAGCCGTGCTTCCCGGCGG
CGCCTGGAGAGCAGCGAAAG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MPPCRLLFRGFHGVIWRSPCSEGSSLMLIAQRPSLAEDPISEFRSRFVIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSI
RVDGVLHEFSTVPGVKEDVTDLILNLKELVVSSDNDEPTVMYLRKQGPGEVTAADIAPPAGVEVHNPDLHLATLNDKGKL
EIELTVERGRGYVSAAQNKQPGQEIGRIPIDSIYSPVLKVTYKVEATRVEQRTDFDRLIVDVETKQSISPRDAMASAGKT
LVGLFGLAQELNAEAEGVDIGPSAADAALAADLALPIEEMDLTVRSYNCLKREGIHTIGELVSRSEADLLDIRNFGQKSI
DEVKTKLGAMGLQLKDSPPGFDPRQAVDTYGTDTYNPSFSDPSDDGREFVETEQY

Sequences:

>Translated_375_residues
MPPCRLLFRGFHGVIWRSPCSEGSSLMLIAQRPSLAEDPISEFRSRFVIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSI
RVDGVLHEFSTVPGVKEDVTDLILNLKELVVSSDNDEPTVMYLRKQGPGEVTAADIAPPAGVEVHNPDLHLATLNDKGKL
EIELTVERGRGYVSAAQNKQPGQEIGRIPIDSIYSPVLKVTYKVEATRVEQRTDFDRLIVDVETKQSISPRDAMASAGKT
LVGLFGLAQELNAEAEGVDIGPSAADAALAADLALPIEEMDLTVRSYNCLKREGIHTIGELVSRSEADLLDIRNFGQKSI
DEVKTKLGAMGLQLKDSPPGFDPRQAVDTYGTDTYNPSFSDPSDDGREFVETEQY
>Mature_374_residues
PPCRLLFRGFHGVIWRSPCSEGSSLMLIAQRPSLAEDPISEFRSRFVIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSIR
VDGVLHEFSTVPGVKEDVTDLILNLKELVVSSDNDEPTVMYLRKQGPGEVTAADIAPPAGVEVHNPDLHLATLNDKGKLE
IELTVERGRGYVSAAQNKQPGQEIGRIPIDSIYSPVLKVTYKVEATRVEQRTDFDRLIVDVETKQSISPRDAMASAGKTL
VGLFGLAQELNAEAEGVDIGPSAADAALAADLALPIEEMDLTVRSYNCLKREGIHTIGELVSRSEADLLDIRNFGQKSID
EVKTKLGAMGLQLKDSPPGFDPRQAVDTYGTDTYNPSFSDPSDDGREFVETEQY

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=321, Percent_Identity=41.7445482866044, Blast_Score=239, Evalue=2e-64,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 40841; Mature: 40709

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPCRLLFRGFHGVIWRSPCSEGSSLMLIAQRPSLAEDPISEFRSRFVIEPLEPGFGYTL
CCHHHHHHHHHCCEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHCEECCCCCCCCCCHH
GNSLRRTLLSSIPGAAVTSIRVDGVLHEFSTVPGVKEDVTDLILNLKELVVSSDNDEPTV
HHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
MYLRKQGPGEVTAADIAPPAGVEVHNPDLHLATLNDKGKLEIELTVERGRGYVSAAQNKQ
EEEEECCCCCEEEECCCCCCCCEEECCCEEEEEECCCCEEEEEEEEECCCCEEEECCCCC
PGQEIGRIPIDSIYSPVLKVTYKVEATRVEQRTDFDRLIVDVETKQSISPRDAMASAGKT
CHHHHCCCCHHHHHHHHHHEEEEEHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCHH
LVGLFGLAQELNAEAEGVDIGPSAADAALAADLALPIEEMDLTVRSYNCLKREGIHTIGE
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHEEHHHHHHHHHHHHHHHHH
LVSRSEADLLDIRNFGQKSIDEVKTKLGAMGLQLKDSPPGFDPRQAVDTYGTDTYNPSFS
HHCCCCCHHHHHHHCCHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHCCCCCCCCCCC
DPSDDGREFVETEQY
CCCCHHHHHHHCCCC
>Mature Secondary Structure 
PPCRLLFRGFHGVIWRSPCSEGSSLMLIAQRPSLAEDPISEFRSRFVIEPLEPGFGYTL
CHHHHHHHHHCCEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHCEECCCCCCCCCCHH
GNSLRRTLLSSIPGAAVTSIRVDGVLHEFSTVPGVKEDVTDLILNLKELVVSSDNDEPTV
HHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
MYLRKQGPGEVTAADIAPPAGVEVHNPDLHLATLNDKGKLEIELTVERGRGYVSAAQNKQ
EEEEECCCCCEEEECCCCCCCCEEECCCEEEEEECCCCEEEEEEEEECCCCEEEECCCCC
PGQEIGRIPIDSIYSPVLKVTYKVEATRVEQRTDFDRLIVDVETKQSISPRDAMASAGKT
CHHHHCCCCHHHHHHHHHHEEEEEHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCHH
LVGLFGLAQELNAEAEGVDIGPSAADAALAADLALPIEEMDLTVRSYNCLKREGIHTIGE
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHEEHHHHHHHHHHHHHHHHH
LVSRSEADLLDIRNFGQKSIDEVKTKLGAMGLQLKDSPPGFDPRQAVDTYGTDTYNPSFS
HHCCCCCHHHHHHHCCHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHCCCCCCCCCCC
DPSDDGREFVETEQY
CCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA