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The map label for this gene is dacD [H]

Identifier: 158317707

GI number: 158317707

Start: 7268303

End: 7269700

Strand: Direct

Name: dacD [H]

Synonym: Franean1_5965

Alternate gene names: 158317707

Gene position: 7268303-7269700 (Clockwise)

Preceding gene: 158317706

Following gene: 158317711

Centisome position: 80.92

GC content: 76.9

Gene sequence:

>1398_bases
GTGCCGCCGCCGGCCGGCATCACCGCCGAGGCGTGGCTCGTCGCGGACGCCGGCAGCGGCGCGATCCTCGCGGCGCACAA
CGCGCACCTCCCGGACCTGCCGGCGAGCACGATGAAGATGCTCACCGCGCTGACCGTGCTCCCCGGGCTCCCGCCAGACC
GTGAGGTGACTATCAGCGACCAGGCCGCCCAGGTGGACGGCACCCGGGTCGGGCTCGTCCCCGGCGTCCGCTACACCGTG
CGCGACCTCGCGACGGCGATGCTGATCGCGAGCGGCAACGACGCCACGGTCGCGCTGGTGGAGGCGGCCGGCGGCCGGGA
GGCCGTACTGGACCGGATGAACCGGCTCGCGGCCTCGCTCGGCGCGTCGGAGACCGTCGCGGGCGATCCCACCGGCCTCG
ACGCCCCCGGTCAGCACACGACGGCCCACGACCTGGCGGTGATCGGGCGGGCAACGCTCAACGACCCCATCGTGCGTGGC
TACCTGACCATCCCGCGTGCCTCGCTGCCGGGCCGCGGCGACGAACGCTTCGAGATCGCGAACCACAACACCCTCATCGG
CAGCTACGAGGGCACCATCGGCATCAAGAACGGCTACACGGTCGCCGCCGGGGCGACGTTCGTCGGCGCGGCGACCAGGA
ACGACCGGACGCTCATCGTCTCGCTACTGCGCACGGCGCCGGCCTACGGGACGGACGCGCGGGCGCTGCTCGACTGGGGC
TTCGCGCACGCGGCCGACGTCCGCCCGGTGGGTCGGCTCGCCGGGCCCTCCGACGTGACACCAGGCGACCCGATCACCGC
CGGTGGGGCCGTGGCCCCCGGCACCGCACCGGGTGGCACCGGCGAGCGCGCCGACAGCCGCGGCGCCGGACAGGACGGCG
GCAGCCCGCCCGGGCGGGCCACCACCGGCGACGCCGGAGGCGTGGGTGCCGTCACCTGGACGGCCCTCGCCGTCACGGTC
GTCGCGACGGCCCTCACCGTGTTCGGGCGGGTCCGCCGCCGGCGCGGCGTGGCCGGGAGGGCCACCGGGCGCACCACCCG
CTCCGGCAGCGGCAGCGCGGGCCGGTGGGATGCAGCGGGCCTCGGGCAGTCCGGCGGACGGCGCGGACGTTCCACGGCCC
CAGGAGGAGTTCCGGGGAGAAGAGGCCACCGAAGAGGCACCAACAGGACGCGGCGGGACGGCGCGGCGGCGCGGGCCCGC
CGGGAGCGGCAGAGCGTCGTCGAGGAGTACCGGGCGACCCGCTCCGCCGCGTCGGCTCCCCGGCCGCGCACCGGCGCCGA
CGGCCAGCGCCATCTCGTTGGTCTGCCCTCGCTGCACCCCGAACCGGGGCGGAGCCGGACCGACTCGTCCCGCACCGACA
CCCCACCCTTCGGCACGGCCCGCTCGCCCCGCCCCTGA

Upstream 100 bases:

>100_bases
CACGTCCGCCGGTACTACCAGCACAGCAGGCACAACTGGCACAGCGGGCGCCACCGACCAGGCGGACACCGCGGAGGCCA
CAGGCGCCGCGGAGACCGCT

Downstream 100 bases:

>100_bases
GCCCGCCCGGTCCCCGAGCTCCCCCAGCTCTGAGGCCGCCTCGCTCTGAGGCCGCCTGACCCCTGAGGCGTGGCGCGCCG
GTCAGCGCCCGACCGGCGCC

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6b; PBP-6b [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MPPPAGITAEAWLVADAGSGAILAAHNAHLPDLPASTMKMLTALTVLPGLPPDREVTISDQAAQVDGTRVGLVPGVRYTV
RDLATAMLIASGNDATVALVEAAGGREAVLDRMNRLAASLGASETVAGDPTGLDAPGQHTTAHDLAVIGRATLNDPIVRG
YLTIPRASLPGRGDERFEIANHNTLIGSYEGTIGIKNGYTVAAGATFVGAATRNDRTLIVSLLRTAPAYGTDARALLDWG
FAHAADVRPVGRLAGPSDVTPGDPITAGGAVAPGTAPGGTGERADSRGAGQDGGSPPGRATTGDAGGVGAVTWTALAVTV
VATALTVFGRVRRRRGVAGRATGRTTRSGSGSAGRWDAAGLGQSGGRRGRSTAPGGVPGRRGHRRGTNRTRRDGAAARAR
RERQSVVEEYRATRSAASAPRPRTGADGQRHLVGLPSLHPEPGRSRTDSSRTDTPPFGTARSPRP

Sequences:

>Translated_465_residues
MPPPAGITAEAWLVADAGSGAILAAHNAHLPDLPASTMKMLTALTVLPGLPPDREVTISDQAAQVDGTRVGLVPGVRYTV
RDLATAMLIASGNDATVALVEAAGGREAVLDRMNRLAASLGASETVAGDPTGLDAPGQHTTAHDLAVIGRATLNDPIVRG
YLTIPRASLPGRGDERFEIANHNTLIGSYEGTIGIKNGYTVAAGATFVGAATRNDRTLIVSLLRTAPAYGTDARALLDWG
FAHAADVRPVGRLAGPSDVTPGDPITAGGAVAPGTAPGGTGERADSRGAGQDGGSPPGRATTGDAGGVGAVTWTALAVTV
VATALTVFGRVRRRRGVAGRATGRTTRSGSGSAGRWDAAGLGQSGGRRGRSTAPGGVPGRRGHRRGTNRTRRDGAAARAR
RERQSVVEEYRATRSAASAPRPRTGADGQRHLVGLPSLHPEPGRSRTDSSRTDTPPFGTARSPRP
>Mature_464_residues
PPPAGITAEAWLVADAGSGAILAAHNAHLPDLPASTMKMLTALTVLPGLPPDREVTISDQAAQVDGTRVGLVPGVRYTVR
DLATAMLIASGNDATVALVEAAGGREAVLDRMNRLAASLGASETVAGDPTGLDAPGQHTTAHDLAVIGRATLNDPIVRGY
LTIPRASLPGRGDERFEIANHNTLIGSYEGTIGIKNGYTVAAGATFVGAATRNDRTLIVSLLRTAPAYGTDARALLDWGF
AHAADVRPVGRLAGPSDVTPGDPITAGGAVAPGTAPGGTGERADSRGAGQDGGSPPGRATTGDAGGVGAVTWTALAVTVV
ATALTVFGRVRRRRGVAGRATGRTTRSGSGSAGRWDAAGLGQSGGRRGRSTAPGGVPGRRGHRRGTNRTRRDGAAARARR
ERQSVVEEYRATRSAASAPRPRTGADGQRHLVGLPSLHPEPGRSRTDSSRTDTPPFGTARSPRP

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=280, Percent_Identity=33.2142857142857, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787062, Length=264, Percent_Identity=31.8181818181818, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1786851, Length=274, Percent_Identity=31.021897810219, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47386; Mature: 47255

Theoretical pI: Translated: 11.70; Mature: 11.70

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPPAGITAEAWLVADAGSGAILAAHNAHLPDLPASTMKMLTALTVLPGLPPDREVTISD
CCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECC
QAAQVDGTRVGLVPGVRYTVRDLATAMLIASGNDATVALVEAAGGREAVLDRMNRLAASL
CHHHCCCCEEECCCCCHHHHHHHHHHHEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHC
GASETVAGDPTGLDAPGQHTTAHDLAVIGRATLNDPIVRGYLTIPRASLPGRGDERFEIA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCEEEEC
NHNTLIGSYEGTIGIKNGYTVAAGATFVGAATRNDRTLIVSLLRTAPAYGTDARALLDWG
CCCEEEEECCCEEEECCCEEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHC
FAHAADVRPVGRLAGPSDVTPGDPITAGGAVAPGTAPGGTGERADSRGAGQDGGSPPGRA
CHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TTGDAGGVGAVTWTALAVTVVATALTVFGRVRRRRGVAGRATGRTTRSGSGSAGRWDAAG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
LGQSGGRRGRSTAPGGVPGRRGHRRGTNRTRRDGAAARARRERQSVVEEYRATRSAASAP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPRTGADGQRHLVGLPSLHPEPGRSRTDSSRTDTPPFGTARSPRP
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PPPAGITAEAWLVADAGSGAILAAHNAHLPDLPASTMKMLTALTVLPGLPPDREVTISD
CCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECC
QAAQVDGTRVGLVPGVRYTVRDLATAMLIASGNDATVALVEAAGGREAVLDRMNRLAASL
CHHHCCCCEEECCCCCHHHHHHHHHHHEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHC
GASETVAGDPTGLDAPGQHTTAHDLAVIGRATLNDPIVRGYLTIPRASLPGRGDERFEIA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCEEEEC
NHNTLIGSYEGTIGIKNGYTVAAGATFVGAATRNDRTLIVSLLRTAPAYGTDARALLDWG
CCCEEEEECCCEEEECCCEEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHC
FAHAADVRPVGRLAGPSDVTPGDPITAGGAVAPGTAPGGTGERADSRGAGQDGGSPPGRA
CHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TTGDAGGVGAVTWTALAVTVVATALTVFGRVRRRRGVAGRATGRTTRSGSGSAGRWDAAG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
LGQSGGRRGRSTAPGGVPGRRGHRRGTNRTRRDGAAARARRERQSVVEEYRATRSAASAP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPRTGADGQRHLVGLPSLHPEPGRSRTDSSRTDTPPFGTARSPRP
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8955390; 9097040; 9278503 [H]