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The map label for this gene is dhbE [H]

Identifier: 158317683

GI number: 158317683

Start: 7238440

End: 7240176

Strand: Reverse

Name: dhbE [H]

Synonym: Franean1_5941

Alternate gene names: 158317683

Gene position: 7240176-7238440 (Counterclockwise)

Preceding gene: 158317684

Following gene: 158317682

Centisome position: 80.61

GC content: 75.13

Gene sequence:

>1737_bases
GTGACCGCCCCGGCGCCGGCGCCGGTGGACGAGGGCTGGACGCGCTGGCCGGCGGAGCTCGAGCGGCGCTACCGGGACGC
GGGGTACTGGGGCGACCAGACCCTGGGTGACCTGCTGCGGGAGTGGGCGCGCCGCTTCGGGCCGGCGACGGCGCTCGTCT
CGGGACCCGACCGGATCAGCTACGCCGAGCTGGACGCGGCCGTGGACGATCTCGCCGCCGGGCTGGCCACCATCGGCATC
GGTCCCGGTGACCACGTGGTGGTGCACCTGCCCAACCGCGCCGAGTTCGTGACCGTGCTGTTCGCGCTGCTGCGCCGGGG
CGCGATCGGCGTCCTCTCGCTGCCCGCCCACCGCAGGGTCGAGATCGAGCACCTCGCGCGGCTCTCGGGCGCCGTCGGCT
ATGTGATCGCCGACCGGCACGAGGGCTTCGACTACCGGGAGCTGGCCCGGGAGGTCACCAAGGCGGTTCCCGCCGTGCGC
CACGTCCTGGTGGCCGGTGAGCCCGGCCCGTTCACCGCGCTGTCGGCGCTCGCCGCGGCCGGGCGCGCCGCCCGGTCCGG
CGGCGGCACACGGACCGGGGCGGCCGGCGGTGCTGTCTCCGTCACCGCGGCCGGCGCCGCTACCGACGACGCCGGTAGCG
GCGGGGCGGGCCCGGATCTCGGCGCCGAGCGACCGGATCCGGGCGGTATCGCGGTGCTGCTGATCTCCGGCGGCACCACC
GGCAAGCCGAAGCTGATCCCGCGCACCCATCGCGACTACGCCTACAACGCCCGCGCCAGCGCGGACGTCTGCGGGCTGAC
GGCGGACGACGTGTACCTCGTCGCGCTGCCCGCCGCCCACAACTTCCCGCTGGCCTGCCCGGGGCTGCTCGGCGCGTTCG
GCGTCGGGGCGACGGTCGTGATGGCTCCCTCCCCGAGTCCGGACGTCGCCTTCGACCTGGTCGCCCGCGAGCGGGTCACC
GTCACCGCGCTCGTCCCCCCGCTGGCCCGGCTGTGGGTCGAGGCGGCCGGGTGGGAGGGGCCGGACACGACGAGCCTGCG
GCTGGTCCAGGTGGGCGGCGCCAAGCTCGACGAGGGCCTGGCGCGGCGGATCACGCCGACCCTCGGGGCGAAGGTCCAGC
AGGTCTTCGGCATGGCCGAGGGCCTGCTGAACTACACCCGCCTCGACGACCCGGACGAGCTCGTGTTCACCACGCAGGGC
CGGCCGCTGGCCGCGGCCGACGAGGTCCGGGTGCTCGACGGGTCCGGTGCCGGGGTGGTCCCGGGCGAGGTGGGCGAGCT
GTGGACCCGCGGGCCGTACACGATCCGCGGGTACTACCGGGCCGCCGAGCACAACTGCGCCGTCTTCGACGGCGACGGGT
ACTTCCGCACCGGCGACCTCGTCCGCCAGCTCCCGACCGGCCACCTCGTGGTCGAGGGCCGGGTCAAGGACGTGATCAAC
CGGGGTGGCGAGAACGTGTCCGCGGGTGAGCTGGAGGAGCACCTGCTGACCCATCCGGCGATCGGGCAGGTGGCGGTCGT
CGGCATGCCGGACCCGGACGTGGGCGAGAGCGTCTGCGCGGTGGTGGTCCTCGCGCCGGGCGAGGCCCTCCGGCTCAAGC
AGGTCAAGAGCTACCTGCAGGAGCGCGGCCTGGCCCGGTTCATGCTCCCCGACCGGCTCGAGGTCGTCGGCGAGTTCCCG
CTCACCGCCGTCGGAAAGATCGACAAGCGGGAGCTGCGGAGCTGGCTGGACCCCTGA

Upstream 100 bases:

>100_bases
TCGCCGCGATCGCACTGGAGATCGCGGCGATGTCGGACGACGAGGTCGCGGCCGAGCTGGTCGACCCGGACACGCTCCCC
GCCGACTCCGGCGGTGCGTC

Downstream 100 bases:

>100_bases
GCCTTCCGCTCTGACATCGACGGCGCTCGCCCCTGCCTCCCGGCGGGCGCCGTCGGAACATCGCCGACCATCGCATTTTT
CTACCCGTCTCCCGCACCGC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MTAPAPAPVDEGWTRWPAELERRYRDAGYWGDQTLGDLLREWARRFGPATALVSGPDRISYAELDAAVDDLAAGLATIGI
GPGDHVVVHLPNRAEFVTVLFALLRRGAIGVLSLPAHRRVEIEHLARLSGAVGYVIADRHEGFDYRELAREVTKAVPAVR
HVLVAGEPGPFTALSALAAAGRAARSGGGTRTGAAGGAVSVTAAGAATDDAGSGGAGPDLGAERPDPGGIAVLLISGGTT
GKPKLIPRTHRDYAYNARASADVCGLTADDVYLVALPAAHNFPLACPGLLGAFGVGATVVMAPSPSPDVAFDLVARERVT
VTALVPPLARLWVEAAGWEGPDTTSLRLVQVGGAKLDEGLARRITPTLGAKVQQVFGMAEGLLNYTRLDDPDELVFTTQG
RPLAAADEVRVLDGSGAGVVPGEVGELWTRGPYTIRGYYRAAEHNCAVFDGDGYFRTGDLVRQLPTGHLVVEGRVKDVIN
RGGENVSAGELEEHLLTHPAIGQVAVVGMPDPDVGESVCAVVVLAPGEALRLKQVKSYLQERGLARFMLPDRLEVVGEFP
LTAVGKIDKRELRSWLDP

Sequences:

>Translated_578_residues
MTAPAPAPVDEGWTRWPAELERRYRDAGYWGDQTLGDLLREWARRFGPATALVSGPDRISYAELDAAVDDLAAGLATIGI
GPGDHVVVHLPNRAEFVTVLFALLRRGAIGVLSLPAHRRVEIEHLARLSGAVGYVIADRHEGFDYRELAREVTKAVPAVR
HVLVAGEPGPFTALSALAAAGRAARSGGGTRTGAAGGAVSVTAAGAATDDAGSGGAGPDLGAERPDPGGIAVLLISGGTT
GKPKLIPRTHRDYAYNARASADVCGLTADDVYLVALPAAHNFPLACPGLLGAFGVGATVVMAPSPSPDVAFDLVARERVT
VTALVPPLARLWVEAAGWEGPDTTSLRLVQVGGAKLDEGLARRITPTLGAKVQQVFGMAEGLLNYTRLDDPDELVFTTQG
RPLAAADEVRVLDGSGAGVVPGEVGELWTRGPYTIRGYYRAAEHNCAVFDGDGYFRTGDLVRQLPTGHLVVEGRVKDVIN
RGGENVSAGELEEHLLTHPAIGQVAVVGMPDPDVGESVCAVVVLAPGEALRLKQVKSYLQERGLARFMLPDRLEVVGEFP
LTAVGKIDKRELRSWLDP
>Mature_577_residues
TAPAPAPVDEGWTRWPAELERRYRDAGYWGDQTLGDLLREWARRFGPATALVSGPDRISYAELDAAVDDLAAGLATIGIG
PGDHVVVHLPNRAEFVTVLFALLRRGAIGVLSLPAHRRVEIEHLARLSGAVGYVIADRHEGFDYRELAREVTKAVPAVRH
VLVAGEPGPFTALSALAAAGRAARSGGGTRTGAAGGAVSVTAAGAATDDAGSGGAGPDLGAERPDPGGIAVLLISGGTTG
KPKLIPRTHRDYAYNARASADVCGLTADDVYLVALPAAHNFPLACPGLLGAFGVGATVVMAPSPSPDVAFDLVARERVTV
TALVPPLARLWVEAAGWEGPDTTSLRLVQVGGAKLDEGLARRITPTLGAKVQQVFGMAEGLLNYTRLDDPDELVFTTQGR
PLAAADEVRVLDGSGAGVVPGEVGELWTRGPYTIRGYYRAAEHNCAVFDGDGYFRTGDLVRQLPTGHLVVEGRVKDVINR
GGENVSAGELEEHLLTHPAIGQVAVVGMPDPDVGESVCAVVVLAPGEALRLKQVKSYLQERGLARFMLPDRLEVVGEFPL
TAVGKIDKRELRSWLDP

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=561, Percent_Identity=24.9554367201426, Blast_Score=143, Evalue=5e-34,
Organism=Homo sapiens, GI187761345, Length=382, Percent_Identity=31.413612565445, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI187761343, Length=382, Percent_Identity=31.413612565445, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI38505220, Length=361, Percent_Identity=26.3157894736842, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI115511026, Length=531, Percent_Identity=24.2937853107345, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI13325057, Length=368, Percent_Identity=30.4347826086957, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI58082049, Length=584, Percent_Identity=23.458904109589, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI157311624, Length=585, Percent_Identity=23.2478632478632, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI157311622, Length=585, Percent_Identity=23.2478632478632, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI28416953, Length=583, Percent_Identity=23.8421955403087, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI42544132, Length=367, Percent_Identity=23.9782016348774, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI122937307, Length=239, Percent_Identity=29.2887029288703, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI8923896, Length=125, Percent_Identity=32.8, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI253970489, Length=125, Percent_Identity=32.8, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1786810, Length=568, Percent_Identity=42.7816901408451, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI145693145, Length=347, Percent_Identity=32.5648414985591, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI221142682, Length=553, Percent_Identity=26.9439421338156, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1788107, Length=575, Percent_Identity=26.0869565217391, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI1789201, Length=377, Percent_Identity=26.2599469496021, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790505, Length=549, Percent_Identity=24.5901639344262, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1786529, Length=567, Percent_Identity=23.8095238095238, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=561, Percent_Identity=27.4509803921569, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71994703, Length=564, Percent_Identity=25.531914893617, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71994694, Length=564, Percent_Identity=25.531914893617, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71994690, Length=564, Percent_Identity=25.531914893617, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17531443, Length=372, Percent_Identity=28.2258064516129, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=534, Percent_Identity=24.3445692883895, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=364, Percent_Identity=27.4725274725275, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559526, Length=361, Percent_Identity=27.7008310249307, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=368, Percent_Identity=27.7173913043478, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=395, Percent_Identity=25.0632911392405, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32563687, Length=357, Percent_Identity=25.7703081232493, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=356, Percent_Identity=26.685393258427, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71982997, Length=546, Percent_Identity=25.4578754578755, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71983001, Length=546, Percent_Identity=25.4578754578755, Blast_Score=79, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=344, Percent_Identity=29.3604651162791, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6319264, Length=560, Percent_Identity=23.3928571428571, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24581924, Length=562, Percent_Identity=25.6227758007117, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI18859661, Length=349, Percent_Identity=28.3667621776504, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21355181, Length=562, Percent_Identity=22.4199288256228, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI281365686, Length=331, Percent_Identity=23.5649546827795, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922652, Length=351, Percent_Identity=25.3561253561254, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21358303, Length=264, Percent_Identity=23.1060606060606, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356441, Length=324, Percent_Identity=25.6172839506173, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21356947, Length=521, Percent_Identity=21.4971209213052, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648253, Length=357, Percent_Identity=23.5294117647059, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24667959, Length=127, Percent_Identity=35.4330708661417, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24667955, Length=127, Percent_Identity=35.4330708661417, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI62472339, Length=127, Percent_Identity=35.4330708661417, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24656500, Length=567, Percent_Identity=22.5749559082892, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648260, Length=353, Percent_Identity=22.9461756373938, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648255, Length=350, Percent_Identity=24, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.-

Molecular weight: Translated: 60765; Mature: 60633

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPAPAPVDEGWTRWPAELERRYRDAGYWGDQTLGDLLREWARRFGPATALVSGPDRIS
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCC
YAELDAAVDDLAAGLATIGIGPGDHVVVHLPNRAEFVTVLFALLRRGAIGVLSLPAHRRV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCC
EIEHLARLSGAVGYVIADRHEGFDYRELAREVTKAVPAVRHVLVAGEPGPFTALSALAAA
CHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHH
GRAARSGGGTRTGAAGGAVSVTAAGAATDDAGSGGAGPDLGAERPDPGGIAVLLISGGTT
HHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
GKPKLIPRTHRDYAYNARASADVCGLTADDVYLVALPAAHNFPLACPGLLGAFGVGATVV
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCEEEE
MAPSPSPDVAFDLVARERVTVTALVPPLARLWVEAAGWEGPDTTSLRLVQVGGAKLDEGL
ECCCCCCCCCCEEHHCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHH
ARRITPTLGAKVQQVFGMAEGLLNYTRLDDPDELVFTTQGRPLAAADEVRVLDGSGAGVV
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCC
PGEVGELWTRGPYTIRGYYRAAEHNCAVFDGDGYFRTGDLVRQLPTGHLVVEGRVKDVIN
CCCHHHHHCCCCEEEEEEEEECCCCEEEECCCCCEECCHHHHHCCCCCEEEECHHHHHHH
RGGENVSAGELEEHLLTHPAIGQVAVVGMPDPDVGESVCAVVVLAPGEALRLKQVKSYLQ
CCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHH
ERGLARFMLPDRLEVVGEFPLTAVGKIDKRELRSWLDP
HCCCEEECCCHHHHHHHCCCCHHHCCCCHHHHHHHCCC
>Mature Secondary Structure 
TAPAPAPVDEGWTRWPAELERRYRDAGYWGDQTLGDLLREWARRFGPATALVSGPDRIS
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCC
YAELDAAVDDLAAGLATIGIGPGDHVVVHLPNRAEFVTVLFALLRRGAIGVLSLPAHRRV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCC
EIEHLARLSGAVGYVIADRHEGFDYRELAREVTKAVPAVRHVLVAGEPGPFTALSALAAA
CHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHH
GRAARSGGGTRTGAAGGAVSVTAAGAATDDAGSGGAGPDLGAERPDPGGIAVLLISGGTT
HHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
GKPKLIPRTHRDYAYNARASADVCGLTADDVYLVALPAAHNFPLACPGLLGAFGVGATVV
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCEEEE
MAPSPSPDVAFDLVARERVTVTALVPPLARLWVEAAGWEGPDTTSLRLVQVGGAKLDEGL
ECCCCCCCCCCEEHHCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHH
ARRITPTLGAKVQQVFGMAEGLLNYTRLDDPDELVFTTQGRPLAAADEVRVLDGSGAGVV
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCC
PGEVGELWTRGPYTIRGYYRAAEHNCAVFDGDGYFRTGDLVRQLPTGHLVVEGRVKDVIN
CCCHHHHHCCCCEEEEEEEEECCCCEEEECCCCCEECCHHHHHCCCCCEEEECHHHHHHH
RGGENVSAGELEEHLLTHPAIGQVAVVGMPDPDVGESVCAVVVLAPGEALRLKQVKSYLQ
CCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHH
ERGLARFMLPDRLEVVGEFPLTAVGKIDKRELRSWLDP
HCCCEEECCCHHHHHHHCCCCHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]