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The map label for this gene is cofE [H]

Identifier: 158317613

GI number: 158317613

Start: 7122275

End: 7123855

Strand: Direct

Name: cofE [H]

Synonym: Franean1_5869

Alternate gene names: 158317613

Gene position: 7122275-7123855 (Clockwise)

Preceding gene: 158317612

Following gene: 158317614

Centisome position: 79.29

GC content: 74.95

Gene sequence:

>1581_bases
GTGTCTGACCTGTCCCCGGTGGGGACATCCATGACGAAGGACGGACCGGAACCCGCGGAGGCGTCGTTCCCGGGCGTCGC
CGCACCCGAGGCGGAAGGTCCGCCCCCGGTCGGTGGCGAAGCCCCGGCCGGGGAACCTCCCGCGGCGGAGGACGCGCTGC
CGGCCGAGCAGTCCGAGAAGTTCGAGAACTTCGAGAAGTCCGACGAGCAGGTCGGCACGGGCGCGGCGGAGCAGGTCGGC
GAGGAGCCGCTGGCGGAGCGGGAGCTGCGGATTCTGCCGCTGGGCGGGATCGGCGAGGTGCGGCCGGGCGACGACCTCGC
CGCGCTGATCGCCGTGGCCGCGGCCACGGGCGCCGGGCCCGGAGGCGGCCCCGGGCTGCGTGACGGTGACGTCCTCGTCG
TGACGTCCAAGATCGTGTCGAAGGCCGAGGACCGGCTGATCCGGATCGAAGGCGACAGAGAGGCCACGCGCCAGGCCGCC
ATCGACGCCGAGTCCGTGCGTGAGGTCGCGCGCCGCGGCCCGACCCGCATCGTCGAGACGCACCACGGCCTCGTGCTGGC
GAGCGCGGGGGTGGACGCGTCCAACATCGCGAAGGACTCGATCGCGCTGCTGCCGGTCGACCCCGATACCAGTGCCCGCA
CGCTGCGGGACGGCCTGCGCGACCGGCTCGGCGTCGACGTCGCGGTGATCATCAGCGACACGGCCGGGCGGCCCTGGCGG
CGCGGGCTGACCGACATGGCGATCGGCGTCGCCGGGATGGCGGCGCTGCGCAGCCACATCGGCGACGTCGACTCCTACGG
CAACGAGCTCGGGATGACCGAGATCGCCGAGGCCGACGAGCTGGCCGGCGCGGCGGACCTGGTGAAGGGCAAACTCGGCG
CCACGCCGGTGGCGATCGTCCGCGGGTTCGCGCGCCGGCCCGACGACGGGAGCGGGTCACGCGCGCTGCTGCGGCCCTGG
GACGAGGACATGTTCCAGCTCGGCACGGTCGAGGCACGCCGCTCGGCCCCGTTCGCGCGGCGCACGGTGCGGGAGTTCGC
CGACCTCCCGGTCGACCCGGCGGCGGTGCACCAGGCGATCGCCGCGGCGATCACCGCGCCCGCCCCGCACCACACCACAC
CATGGCGGTTCGTGCTGGTCGAGGCGCGCCGGCACGCCCTGCTCGACGCGATGGCGATGGCCTGGACGGACGACCTGCGC
CGCGACGGGTTCGACGAGGACTCGGTGAGCAGGCGGCTGCGCCGCGGCGACGTGCTGCGCCGGGCGCCGGTGCTGATCGT
CCCGATGATGGTGACCGACGGCGCGCATCCCTACCCGGACGAGCGTCGGGTGCGGGCCGAGGAGCGGATGTTCACCGTGG
CGCTCGGCGCCGCCGTGCAGAACCTGCTGGTCGCCCTCGCGGCCGAGGGCCTGGGCTCGTGCTGGGTGTCGTCCGCGCTG
TTCTGCGGGGAGGTCGTGACCGACACGCTGGACCTGCCGCGCACCTGGACGCCGATGGGCGTCGTGGGTGTGGGGCACCC
GGCCGCACCGGCGCACCCGCGGCCGGCCCGTGACATCGCGGCGTTCGTCGTGGAGCGCTGA

Upstream 100 bases:

>100_bases
CCCGACGCCGTACCCGGAGCTACCGTGAAAGCCGTACCGCTTCTCATGACCGATCTGGAGGCCACTGTGGCGATCGTGGA
GGCGGCGCTGGAGCTCGCCG

Downstream 100 bases:

>100_bases
GCCGGGGCCGGCGTCGCGGTACGGCCGCGCCGACGTCCGCCGGCGCGGAGCGGCCGGGGCCATGGTCAACGGCGCGGGGG
TCTTCCGCTCGCCGGCCGGA

Product: F420-0--gamma-glutamyl ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSDLSPVGTSMTKDGPEPAEASFPGVAAPEAEGPPPVGGEAPAGEPPAAEDALPAEQSEKFENFEKSDEQVGTGAAEQVG
EEPLAERELRILPLGGIGEVRPGDDLAALIAVAAATGAGPGGGPGLRDGDVLVVTSKIVSKAEDRLIRIEGDREATRQAA
IDAESVREVARRGPTRIVETHHGLVLASAGVDASNIAKDSIALLPVDPDTSARTLRDGLRDRLGVDVAVIISDTAGRPWR
RGLTDMAIGVAGMAALRSHIGDVDSYGNELGMTEIAEADELAGAADLVKGKLGATPVAIVRGFARRPDDGSGSRALLRPW
DEDMFQLGTVEARRSAPFARRTVREFADLPVDPAAVHQAIAAAITAPAPHHTTPWRFVLVEARRHALLDAMAMAWTDDLR
RDGFDEDSVSRRLRRGDVLRRAPVLIVPMMVTDGAHPYPDERRVRAEERMFTVALGAAVQNLLVALAAEGLGSCWVSSAL
FCGEVVTDTLDLPRTWTPMGVVGVGHPAAPAHPRPARDIAAFVVER

Sequences:

>Translated_526_residues
MSDLSPVGTSMTKDGPEPAEASFPGVAAPEAEGPPPVGGEAPAGEPPAAEDALPAEQSEKFENFEKSDEQVGTGAAEQVG
EEPLAERELRILPLGGIGEVRPGDDLAALIAVAAATGAGPGGGPGLRDGDVLVVTSKIVSKAEDRLIRIEGDREATRQAA
IDAESVREVARRGPTRIVETHHGLVLASAGVDASNIAKDSIALLPVDPDTSARTLRDGLRDRLGVDVAVIISDTAGRPWR
RGLTDMAIGVAGMAALRSHIGDVDSYGNELGMTEIAEADELAGAADLVKGKLGATPVAIVRGFARRPDDGSGSRALLRPW
DEDMFQLGTVEARRSAPFARRTVREFADLPVDPAAVHQAIAAAITAPAPHHTTPWRFVLVEARRHALLDAMAMAWTDDLR
RDGFDEDSVSRRLRRGDVLRRAPVLIVPMMVTDGAHPYPDERRVRAEERMFTVALGAAVQNLLVALAAEGLGSCWVSSAL
FCGEVVTDTLDLPRTWTPMGVVGVGHPAAPAHPRPARDIAAFVVER
>Mature_525_residues
SDLSPVGTSMTKDGPEPAEASFPGVAAPEAEGPPPVGGEAPAGEPPAAEDALPAEQSEKFENFEKSDEQVGTGAAEQVGE
EPLAERELRILPLGGIGEVRPGDDLAALIAVAAATGAGPGGGPGLRDGDVLVVTSKIVSKAEDRLIRIEGDREATRQAAI
DAESVREVARRGPTRIVETHHGLVLASAGVDASNIAKDSIALLPVDPDTSARTLRDGLRDRLGVDVAVIISDTAGRPWRR
GLTDMAIGVAGMAALRSHIGDVDSYGNELGMTEIAEADELAGAADLVKGKLGATPVAIVRGFARRPDDGSGSRALLRPWD
EDMFQLGTVEARRSAPFARRTVREFADLPVDPAAVHQAIAAAITAPAPHHTTPWRFVLVEARRHALLDAMAMAWTDDLRR
DGFDEDSVSRRLRRGDVLRRAPVLIVPMMVTDGAHPYPDERRVRAEERMFTVALGAAVQNLLVALAAEGLGSCWVSSALF
CGEVVTDTLDLPRTWTPMGVVGVGHPAAPAHPRPARDIAAFVVER

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225
- InterPro:   IPR019943
- InterPro:   IPR000415 [H]

Pfam domain/function: PF01996 F420_ligase; PF00881 Nitroreductase [H]

EC number: NA

Molecular weight: Translated: 55439; Mature: 55308

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLSPVGTSMTKDGPEPAEASFPGVAAPEAEGPPPVGGEAPAGEPPAAEDALPAEQSEK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
FENFEKSDEQVGTGAAEQVGEEPLAERELRILPLGGIGEVRPGDDLAALIAVAAATGAGP
HHHHHHHHHHHCCCHHHHHCCCCHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
GGGPGLRDGDVLVVTSKIVSKAEDRLIRIEGDREATRQAAIDAESVREVARRGPTRIVET
CCCCCCCCCCEEEEEHHHHHHHHCCEEEEECCHHHHHHHHCCHHHHHHHHHCCCCEEEEC
HHGLVLASAGVDASNIAKDSIALLPVDPDTSARTLRDGLRDRLGVDVAVIISDTAGRPWR
CCCEEEEECCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
RGLTDMAIGVAGMAALRSHIGDVDSYGNELGMTEIAEADELAGAADLVKGKLGATPVAIV
HCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCHHHHHHCCCCCCHHHHH
RGFARRPDDGSGSRALLRPWDEDMFQLGTVEARRSAPFARRTVREFADLPVDPAAVHQAI
HHHHCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHH
AAAITAPAPHHTTPWRFVLVEARRHALLDAMAMAWTDDLRRDGFDEDSVSRRLRRGDVLR
HHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RAPVLIVPMMVTDGAHPYPDERRVRAEERMFTVALGAAVQNLLVALAAEGLGSCWVSSAL
HCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FCGEVVTDTLDLPRTWTPMGVVGVGHPAAPAHPRPARDIAAFVVER
HHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SDLSPVGTSMTKDGPEPAEASFPGVAAPEAEGPPPVGGEAPAGEPPAAEDALPAEQSEK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
FENFEKSDEQVGTGAAEQVGEEPLAERELRILPLGGIGEVRPGDDLAALIAVAAATGAGP
HHHHHHHHHHHCCCHHHHHCCCCHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
GGGPGLRDGDVLVVTSKIVSKAEDRLIRIEGDREATRQAAIDAESVREVARRGPTRIVET
CCCCCCCCCCEEEEEHHHHHHHHCCEEEEECCHHHHHHHHCCHHHHHHHHHCCCCEEEEC
HHGLVLASAGVDASNIAKDSIALLPVDPDTSARTLRDGLRDRLGVDVAVIISDTAGRPWR
CCCEEEEECCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
RGLTDMAIGVAGMAALRSHIGDVDSYGNELGMTEIAEADELAGAADLVKGKLGATPVAIV
HCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCHHHHHHCCCCCCHHHHH
RGFARRPDDGSGSRALLRPWDEDMFQLGTVEARRSAPFARRTVREFADLPVDPAAVHQAI
HHHHCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHH
AAAITAPAPHHTTPWRFVLVEARRHALLDAMAMAWTDDLRRDGFDEDSVSRRLRRGDVLR
HHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RAPVLIVPMMVTDGAHPYPDERRVRAEERMFTVALGAAVQNLLVALAAEGLGSCWVSSAL
HCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FCGEVVTDTLDLPRTWTPMGVVGVGHPAAPAHPRPARDIAAFVVER
HHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]