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The map label for this gene is pbpF [H]

Identifier: 158317597

GI number: 158317597

Start: 7100804

End: 7103263

Strand: Direct

Name: pbpF [H]

Synonym: Franean1_5853

Alternate gene names: 158317597

Gene position: 7100804-7103263 (Clockwise)

Preceding gene: 158317596

Following gene: 158317598

Centisome position: 79.06

GC content: 72.07

Gene sequence:

>2460_bases
ATGGCTGGCCGGCCGCGGGGTGGTGTCACCCACCCGGACGAGACAGGTGGTCCCGCCGGGCGGTCCCGCGGTGGGCCGAA
CGGGCCCGGGCGGCTCGGGCGGTCGGTGCCGCGTCCCCCGGGGAGCCGTTCACGCGGCACGTCGGCGCCGCTCGGCAGCG
CCGACGACCTGCCGTTCGGGCCGCCGACGGGCGGAATTCGCCGCCCAGTCGACCGGCGGCGGCACCTCACCCGGGCGGGG
CTCATCGCGACCGCCATGGGCGTCGCGGTCGGCCTCATGGCGTTGCCGTTCCTGAGCGCCGCGGGGCTGTTCGCGAAGGC
GTCCGCCGACCACTTCCTGGAACTTCCGGCGGACCTCGTCATCCCGCCGCCGCCACAGAGCTCCAAGATCCTCGCGGCGG
ACGGATCCGTGATCGCCACGCTCACCGGTACAGAGAACCGGGAGGTCGTCACCGGCGACCAGATCCCGAAGATCATGCGG
GAGGCGATCGTCTCGATCGAGGACGCCCGCTTCTACTCTCACAACGGTGTCGACCCCCAGGGGGTGTTGCGGGCCGCGGC
CCGCAACAGCGAGGCCGGCTCCACCACCCAGGGCGGCTCCACACTGACCCAGCAGTACGTGAAGAACGTGCTGCTGCAGG
ACGCCACCACTCCCGAGGAGCGCGACGCGGTCGCCGGCGACTCGCTGGACCGCAAGCTGCGCGAGCTCCGCTACGCGATC
GCGATCGAGCAGCGGTTCAGCAAGGACGAGATCCTCACCCGGTACCTGAACATCGCCTACTTCGGCGACGGCGCGTACGG
CGTGGGGACGGCGGCCGAGCACTACTTCGGCATTCCCATCTCCGAGGTCAGCCTCGAGCAGGCCGCCCTGCTCGCCGGGC
TCGTCCAGAGCCCGTCGCGCTACGACCCGGTGAACCACCCGGAGGCCGCACTCGAGCGCAGGAACATGGTGCTCACCCGG
ATGGCCGACGAGCACTACGTCAGCCGCACCCAGGCGGACGCCGCCAGGCAGATGCCGATCGACGTCATCCGCGACCAGCC
GACCACGCAGGACTCCTGTGAGATCTCGGTGGCGCCGTTCTTCTGCGACTACATCCGCACCCGGCTGCGCGCCAACCCGG
CGCTGGGCGCCACCGCCGAGGAGCGTGACCGGCGCATCCACGAGGGTGGCCTGGTCATCAAGACCACGCTGAACCCGCAG
GTGCAGCAGGCGGCGCAGATGGCGGTCAACTCGACCATCCCGCCGGACAACCGGGTGTCCGCGACCGAGGTCGTCATCCA
GCCGGGCACCGGCGCGATCCTCGGGATGGCGGTCAACCGCGTCTACGGGACCAACGAGGCGGCGAACCAGACGAAGGTCT
CGCTGCCGATGGAGCCCACGTTCCAGCCCGGGTCGACCTTCAAGACGTTCACCGTGGCGGCCGCACTCGAGCAGGGGTAC
GGCGTGGGGACGGCGTGGTACTCCCCCGCCTGCTACTCGACCGACGCTTTCCCGCTCGACCGCGGCGAGGGGGACTGCCC
CAAGGGCTACCAGAACGCCGACCCGGCCGAAGCCGGCATCTACCGGATGGACGACGCGACCTGGGATTCGGTCAACACCT
ACTTCATCCAGCTCGAGGAGGAGGTCGGGGTGCCGGCGGTCGTCGAGATGGCGACCAGGCTCGGCGTCTCCCCCGACCAC
CTCAAGGACATCCCGCCGACCAAGGGCGACGTCACCATCGGCGGTGAGTACGTCAGCCCGATGGACATGGCGGTCGCCTA
CTCCACGCTGGCGGCCGGCGGCGTCCGGTGCGAGCCGCGCTTCGCCACCTCGGTGGTCGACGCCGGTGGCCAGCAGATCG
ACGTGGGCAACACGCCGAAGTGCGAGCGGGTGCTGTCCCAGGGCGTCGCGGACACCACGACGAGCATCCTGGCCGGGGTG
CTCACCAAGGGCACCGGCGGGAACGCCCGGTTCGACCACCCCGCGGCGGGCAAGACCGGCACCAACGACGGCTTCTCCAG
CGCCTGGTTCGTCGGCTACACCCCGCAGATCGCGGCGGCGGTGGCCGTTGGCGACCCGCACGGCGCGGTCGCGCACCCGC
TGCGCAACGTCGTCGCGGCCGGCCGCACCTGGCCGCACGTGTTCGGCGGCGACCTGCCGGCGATCATCTGGGGCAGTTCG
ATGCGGGGCGCGCTGGCCGGGCAGCCCGTGCAGCCGCTGCCCGGCGCGGACCGCGACGTGGCCCGGGGAACCAGGGGCGG
CCGACTCATGAACACCCCACCACCGGCACCCACCCCGACGCTGCCGAGCCTGGAAGATCTGCTGCCAGGCGCGGGCACCG
GCCAGACCCCGGGGTTCGGGCAGGGCGGCGGTGGCGGTGGTCAGGGCCCGGGGCAGGTCGTCGTGCCGCAGACCAACCAG
GGCACCGGCCAGGGGCAACAACAGCAGGGCCAACCCGGCCGCAACAACCAGGGCCGCTAG

Upstream 100 bases:

>100_bases
GGGGGTGACAGCGACGGTACAGGGACAGGAGTCGGCATCCCCGGTCCGCGGTGACGCCGCGTCCGGTCGTGCCGGACGCA
GGAGAGGCCCGCAGGTCCGC

Downstream 100 bases:

>100_bases
GCGTCCCACGGCCCGGGGCCCGAGCGGAATCCCGCCGACAGGTCCCGGGCCTGGTGACCCCCAGGTGGCCCGTCCGGGAA
AACCGGTGGCGGCGGGCGGG

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 819; Mature: 818

Protein sequence:

>819_residues
MAGRPRGGVTHPDETGGPAGRSRGGPNGPGRLGRSVPRPPGSRSRGTSAPLGSADDLPFGPPTGGIRRPVDRRRHLTRAG
LIATAMGVAVGLMALPFLSAAGLFAKASADHFLELPADLVIPPPPQSSKILAADGSVIATLTGTENREVVTGDQIPKIMR
EAIVSIEDARFYSHNGVDPQGVLRAAARNSEAGSTTQGGSTLTQQYVKNVLLQDATTPEERDAVAGDSLDRKLRELRYAI
AIEQRFSKDEILTRYLNIAYFGDGAYGVGTAAEHYFGIPISEVSLEQAALLAGLVQSPSRYDPVNHPEAALERRNMVLTR
MADEHYVSRTQADAARQMPIDVIRDQPTTQDSCEISVAPFFCDYIRTRLRANPALGATAEERDRRIHEGGLVIKTTLNPQ
VQQAAQMAVNSTIPPDNRVSATEVVIQPGTGAILGMAVNRVYGTNEAANQTKVSLPMEPTFQPGSTFKTFTVAAALEQGY
GVGTAWYSPACYSTDAFPLDRGEGDCPKGYQNADPAEAGIYRMDDATWDSVNTYFIQLEEEVGVPAVVEMATRLGVSPDH
LKDIPPTKGDVTIGGEYVSPMDMAVAYSTLAAGGVRCEPRFATSVVDAGGQQIDVGNTPKCERVLSQGVADTTTSILAGV
LTKGTGGNARFDHPAAGKTGTNDGFSSAWFVGYTPQIAAAVAVGDPHGAVAHPLRNVVAAGRTWPHVFGGDLPAIIWGSS
MRGALAGQPVQPLPGADRDVARGTRGGRLMNTPPPAPTPTLPSLEDLLPGAGTGQTPGFGQGGGGGGQGPGQVVVPQTNQ
GTGQGQQQQGQPGRNNQGR

Sequences:

>Translated_819_residues
MAGRPRGGVTHPDETGGPAGRSRGGPNGPGRLGRSVPRPPGSRSRGTSAPLGSADDLPFGPPTGGIRRPVDRRRHLTRAG
LIATAMGVAVGLMALPFLSAAGLFAKASADHFLELPADLVIPPPPQSSKILAADGSVIATLTGTENREVVTGDQIPKIMR
EAIVSIEDARFYSHNGVDPQGVLRAAARNSEAGSTTQGGSTLTQQYVKNVLLQDATTPEERDAVAGDSLDRKLRELRYAI
AIEQRFSKDEILTRYLNIAYFGDGAYGVGTAAEHYFGIPISEVSLEQAALLAGLVQSPSRYDPVNHPEAALERRNMVLTR
MADEHYVSRTQADAARQMPIDVIRDQPTTQDSCEISVAPFFCDYIRTRLRANPALGATAEERDRRIHEGGLVIKTTLNPQ
VQQAAQMAVNSTIPPDNRVSATEVVIQPGTGAILGMAVNRVYGTNEAANQTKVSLPMEPTFQPGSTFKTFTVAAALEQGY
GVGTAWYSPACYSTDAFPLDRGEGDCPKGYQNADPAEAGIYRMDDATWDSVNTYFIQLEEEVGVPAVVEMATRLGVSPDH
LKDIPPTKGDVTIGGEYVSPMDMAVAYSTLAAGGVRCEPRFATSVVDAGGQQIDVGNTPKCERVLSQGVADTTTSILAGV
LTKGTGGNARFDHPAAGKTGTNDGFSSAWFVGYTPQIAAAVAVGDPHGAVAHPLRNVVAAGRTWPHVFGGDLPAIIWGSS
MRGALAGQPVQPLPGADRDVARGTRGGRLMNTPPPAPTPTLPSLEDLLPGAGTGQTPGFGQGGGGGGQGPGQVVVPQTNQ
GTGQGQQQQGQPGRNNQGR
>Mature_818_residues
AGRPRGGVTHPDETGGPAGRSRGGPNGPGRLGRSVPRPPGSRSRGTSAPLGSADDLPFGPPTGGIRRPVDRRRHLTRAGL
IATAMGVAVGLMALPFLSAAGLFAKASADHFLELPADLVIPPPPQSSKILAADGSVIATLTGTENREVVTGDQIPKIMRE
AIVSIEDARFYSHNGVDPQGVLRAAARNSEAGSTTQGGSTLTQQYVKNVLLQDATTPEERDAVAGDSLDRKLRELRYAIA
IEQRFSKDEILTRYLNIAYFGDGAYGVGTAAEHYFGIPISEVSLEQAALLAGLVQSPSRYDPVNHPEAALERRNMVLTRM
ADEHYVSRTQADAARQMPIDVIRDQPTTQDSCEISVAPFFCDYIRTRLRANPALGATAEERDRRIHEGGLVIKTTLNPQV
QQAAQMAVNSTIPPDNRVSATEVVIQPGTGAILGMAVNRVYGTNEAANQTKVSLPMEPTFQPGSTFKTFTVAAALEQGYG
VGTAWYSPACYSTDAFPLDRGEGDCPKGYQNADPAEAGIYRMDDATWDSVNTYFIQLEEEVGVPAVVEMATRLGVSPDHL
KDIPPTKGDVTIGGEYVSPMDMAVAYSTLAAGGVRCEPRFATSVVDAGGQQIDVGNTPKCERVLSQGVADTTTSILAGVL
TKGTGGNARFDHPAAGKTGTNDGFSSAWFVGYTPQIAAAVAVGDPHGAVAHPLRNVVAAGRTWPHVFGGDLPAIIWGSSM
RGALAGQPVQPLPGADRDVARGTRGGRLMNTPPPAPTPTLPSLEDLLPGAGTGQTPGFGQGGGGGGQGPGQVVVPQTNQG
TGQGQQQQGQPGRNNQGR

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=291, Percent_Identity=40.2061855670103, Blast_Score=189, Evalue=7e-49,
Organism=Escherichia coli, GI1786343, Length=554, Percent_Identity=31.0469314079422, Blast_Score=175, Evalue=1e-44,
Organism=Escherichia coli, GI1788867, Length=616, Percent_Identity=28.2467532467532, Blast_Score=157, Evalue=3e-39,
Organism=Escherichia coli, GI1789601, Length=188, Percent_Identity=35.6382978723404, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 85873; Mature: 85742

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRPRGGVTHPDETGGPAGRSRGGPNGPGRLGRSVPRPPGSRSRGTSAPLGSADDLPFG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PPTGGIRRPVDRRRHLTRAGLIATAMGVAVGLMALPFLSAAGLFAKASADHFLELPADLV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEE
IPPPPQSSKILAADGSVIATLTGTENREVVTGDQIPKIMREAIVSIEDARFYSHNGVDPQ
ECCCCCCCEEEEECCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHEECCCCCCHH
GVLRAAARNSEAGSTTQGGSTLTQQYVKNVLLQDATTPEERDAVAGDSLDRKLRELRYAI
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHH
AIEQRFSKDEILTRYLNIAYFGDGAYGVGTAAEHYFGIPISEVSLEQAALLAGLVQSPSR
HHHHHCCHHHHHHHHHEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
YDPVNHPEAALERRNMVLTRMADEHYVSRTQADAARQMPIDVIRDQPTTQDSCEISVAPF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEEHHH
FCDYIRTRLRANPALGATAEERDRRIHEGGLVIKTTLNPQVQQAAQMAVNSTIPPDNRVS
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCC
ATEVVIQPGTGAILGMAVNRVYGTNEAANQTKVSLPMEPTFQPGSTFKTFTVAAALEQGY
EEEEEEECCCCEEHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
GVGTAWYSPACYSTDAFPLDRGEGDCPKGYQNADPAEAGIYRMDDATWDSVNTYFIQLEE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEEECCCCCCCCEEEEEEEEC
EVGVPAVVEMATRLGVSPDHLKDIPPTKGDVTIGGEYVSPMDMAVAYSTLAAGGVRCEPR
CCCCHHHHHHHHHCCCCHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCH
FATSVVDAGGQQIDVGNTPKCERVLSQGVADTTTSILAGVLTKGTGGNARFDHPAAGKTG
HHHHHHHCCCCEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
TNDGFSSAWFVGYTPQIAAAVAVGDPHGAVAHPLRNVVAAGRTWPHVFGGDLPAIIWGSS
CCCCCCCCEEEECCHHHEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHCCCCCEEEECCC
MRGALAGQPVQPLPGADRDVARGTRGGRLMNTPPPAPTPTLPSLEDLLPGAGTGQTPGFG
CCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCC
QGGGGGGQGPGQVVVPQTNQGTGQGQQQQGQPGRNNQGR
CCCCCCCCCCCCEEEECCCCCCCCCCHHCCCCCCCCCCC
>Mature Secondary Structure 
AGRPRGGVTHPDETGGPAGRSRGGPNGPGRLGRSVPRPPGSRSRGTSAPLGSADDLPFG
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PPTGGIRRPVDRRRHLTRAGLIATAMGVAVGLMALPFLSAAGLFAKASADHFLELPADLV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEE
IPPPPQSSKILAADGSVIATLTGTENREVVTGDQIPKIMREAIVSIEDARFYSHNGVDPQ
ECCCCCCCEEEEECCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHEECCCCCCHH
GVLRAAARNSEAGSTTQGGSTLTQQYVKNVLLQDATTPEERDAVAGDSLDRKLRELRYAI
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHH
AIEQRFSKDEILTRYLNIAYFGDGAYGVGTAAEHYFGIPISEVSLEQAALLAGLVQSPSR
HHHHHCCHHHHHHHHHEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
YDPVNHPEAALERRNMVLTRMADEHYVSRTQADAARQMPIDVIRDQPTTQDSCEISVAPF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEEHHH
FCDYIRTRLRANPALGATAEERDRRIHEGGLVIKTTLNPQVQQAAQMAVNSTIPPDNRVS
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCC
ATEVVIQPGTGAILGMAVNRVYGTNEAANQTKVSLPMEPTFQPGSTFKTFTVAAALEQGY
EEEEEEECCCCEEHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
GVGTAWYSPACYSTDAFPLDRGEGDCPKGYQNADPAEAGIYRMDDATWDSVNTYFIQLEE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEEECCCCCCCCEEEEEEEEC
EVGVPAVVEMATRLGVSPDHLKDIPPTKGDVTIGGEYVSPMDMAVAYSTLAAGGVRCEPR
CCCCHHHHHHHHHCCCCHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCH
FATSVVDAGGQQIDVGNTPKCERVLSQGVADTTTSILAGVLTKGTGGNARFDHPAAGKTG
HHHHHHHCCCCEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
TNDGFSSAWFVGYTPQIAAAVAVGDPHGAVAHPLRNVVAAGRTWPHVFGGDLPAIIWGSS
CCCCCCCCEEEECCHHHEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHCCCCCEEEECCC
MRGALAGQPVQPLPGADRDVARGTRGGRLMNTPPPAPTPTLPSLEDLLPGAGTGQTPGFG
CCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCC
QGGGGGGQGPGQVVVPQTNQGTGQGQQQQGQPGRNNQGR
CCCCCCCCCCCCEEEECCCCCCCCCCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]