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The map label for this gene is gspE [C]

Identifier: 158317570

GI number: 158317570

Start: 7066441

End: 7067757

Strand: Reverse

Name: gspE [C]

Synonym: Franean1_5826

Alternate gene names: 158317570

Gene position: 7067757-7066441 (Counterclockwise)

Preceding gene: 158317572

Following gene: 158317569

Centisome position: 78.69

GC content: 69.17

Gene sequence:

>1317_bases
GTGAATATGGAAGAGGAGCGGCGATTCTCCGTGGCGGCAGCACGCGGGAACTGGCCGGACGCGACCTCTCACGTGGAAGG
AGAGGTCCGTGAACTGATCCGGCGGCGTTCCCTCGACCCGGAGCGGGAACCCGTCCTCATTCGTCAACTTGTGGACGCGG
TTCTCGTCGACTACGAGGAACGTGCGCTCACCAGTGATCTCCCACCCGTCGCCGACCGGGATGCGACCGCTCGTTCGGTT
TATGACGCCGTCGCCGGTTTCGGGCCGCTTCAACGCCATCTCGACGATCCGGCCGTGGAGGAGATCTGGATCAACGAGCC
GGGCCGGGTGTTCGTCGCGCGGCACGGGCGCAGCGAGCTGACCACCACGATTCTCACCCAGGACCAGGTGCAGGACCTGG
TGGAACGCATGTTGAAGTCGTCCGGGCGCCGGGTCGACCTCTCCACCCCGTTCGTCGACGCCATGCTTCCCGATGGCTCC
CGCCTGCACGTCGTCATCCCGGACGTCACCCGGGTGCACTGGTCGGTGAACATCCGGAAGTTCGTTCTGTCCGCCGGCAG
CCTCGACGAGCTCGTCGCCCTGGGGACGATCACACCGAACGCCGCGATGTTCCTGGAGGCGTCGGTCATCTCCGGCCTCA
ACGTCATCGTCGCCGGCGGCACCCAGGCCGGGAAGACGACCATGCTCAACTGCCTCGGCTCCGCCATCCCCGCCCGGGAA
CGGGTGATCAGCGTCGAGGAGGTCTTCGAGCTCCGGCTGCGCGCGCCCGACTGGGTGGCCATGCAGACCCGGCAGGCGAA
CCTGGAGGGCACCGGGGAGATCCGGCTGCGCCGGCTCATCAAGGAGGCGCTGCGGATGCGCCCGGACCGGATCCTGGTCG
GCGAGGTGCGCCAGGAGGAGGCGCTCGACCTGCTCATCGCCCTCAACTCCGGCCTGCCCGGAATGTGTAGCCTGCACGCC
AACTCCGCCCGCGAGGCGGTGACCAAGCTGTGCACCCTGCCGCTGCTGGCCGGCGAGAACGTCACCCACTCCTTCGTGGT
CCCCACCGTCGCCTCCAGCGTGGACCTGGTCGTTCACCTGTCGAAGGAGGCGGACGGTCGGCGGCGGGTCACCGAGATCG
CCGCGCTGCCGGGCCGCACCGAGGGCGACACGGTGGAGGTCGCCCAGATCTTCCGGACCCGCGGCAACATGCTCGTGCGC
GCCGACGGGTTCCCGCCCCATCCCGAGCGGTACCTGCGTGCCGGCTTCGACCTGCCGGCGCTGCTCGCTCCCAGCGAGCA
CGAGCGGGCGTACGCGCAGATCGCGGTCGAGCGCTGA

Upstream 100 bases:

>100_bases
GCGAACGTTGTTGCTTGCGTACCTGCTCGGAAGGGTTGATGTCGAGTGACCCGCTTCCTACGCTTCGTAGCTGATGTCCG
AAGCGTGGGGGGCTGGCCGC

Downstream 100 bases:

>100_bases
TGGGCATCTTCCTCGGCCTGCTGTTCGGCCTCGGCCTCTTCCTGATCGTGGCGAGTGGGCGTCCGCGGGCCGGTGGCGCG
CGCGCCGAGGCCTCCTGGGC

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MNMEEERRFSVAAARGNWPDATSHVEGEVRELIRRRSLDPEREPVLIRQLVDAVLVDYEERALTSDLPPVADRDATARSV
YDAVAGFGPLQRHLDDPAVEEIWINEPGRVFVARHGRSELTTTILTQDQVQDLVERMLKSSGRRVDLSTPFVDAMLPDGS
RLHVVIPDVTRVHWSVNIRKFVLSAGSLDELVALGTITPNAAMFLEASVISGLNVIVAGGTQAGKTTMLNCLGSAIPARE
RVISVEEVFELRLRAPDWVAMQTRQANLEGTGEIRLRRLIKEALRMRPDRILVGEVRQEEALDLLIALNSGLPGMCSLHA
NSAREAVTKLCTLPLLAGENVTHSFVVPTVASSVDLVVHLSKEADGRRRVTEIAALPGRTEGDTVEVAQIFRTRGNMLVR
ADGFPPHPERYLRAGFDLPALLAPSEHERAYAQIAVER

Sequences:

>Translated_438_residues
MNMEEERRFSVAAARGNWPDATSHVEGEVRELIRRRSLDPEREPVLIRQLVDAVLVDYEERALTSDLPPVADRDATARSV
YDAVAGFGPLQRHLDDPAVEEIWINEPGRVFVARHGRSELTTTILTQDQVQDLVERMLKSSGRRVDLSTPFVDAMLPDGS
RLHVVIPDVTRVHWSVNIRKFVLSAGSLDELVALGTITPNAAMFLEASVISGLNVIVAGGTQAGKTTMLNCLGSAIPARE
RVISVEEVFELRLRAPDWVAMQTRQANLEGTGEIRLRRLIKEALRMRPDRILVGEVRQEEALDLLIALNSGLPGMCSLHA
NSAREAVTKLCTLPLLAGENVTHSFVVPTVASSVDLVVHLSKEADGRRRVTEIAALPGRTEGDTVEVAQIFRTRGNMLVR
ADGFPPHPERYLRAGFDLPALLAPSEHERAYAQIAVER
>Mature_438_residues
MNMEEERRFSVAAARGNWPDATSHVEGEVRELIRRRSLDPEREPVLIRQLVDAVLVDYEERALTSDLPPVADRDATARSV
YDAVAGFGPLQRHLDDPAVEEIWINEPGRVFVARHGRSELTTTILTQDQVQDLVERMLKSSGRRVDLSTPFVDAMLPDGS
RLHVVIPDVTRVHWSVNIRKFVLSAGSLDELVALGTITPNAAMFLEASVISGLNVIVAGGTQAGKTTMLNCLGSAIPARE
RVISVEEVFELRLRAPDWVAMQTRQANLEGTGEIRLRRLIKEALRMRPDRILVGEVRQEEALDLLIALNSGLPGMCSLHA
NSAREAVTKLCTLPLLAGENVTHSFVVPTVASSVDLVVHLSKEADGRRRVTEIAALPGRTEGDTVEVAQIFRTRGNMLVR
ADGFPPHPERYLRAGFDLPALLAPSEHERAYAQIAVER

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 48343; Mature: 48343

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMEEERRFSVAAARGNWPDATSHVEGEVRELIRRRSLDPEREPVLIRQLVDAVLVDYEE
CCCCHHHHEEEEEECCCCCCCCHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHH
RALTSDLPPVADRDATARSVYDAVAGFGPLQRHLDDPAVEEIWINEPGRVFVARHGRSEL
HHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCHHHEEECCCCEEEEEECCCCHH
TTTILTQDQVQDLVERMLKSSGRRVDLSTPFVDAMLPDGSRLHVVIPDVTRVHWSVNIRK
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHCCCCCCEEEEEECCCEEEEEEEEHHH
FVLSAGSLDELVALGTITPNAAMFLEASVISGLNVIVAGGTQAGKTTMLNCLGSAIPARE
HHCCCCCHHHHHEEECCCCCHHHEEEHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCHHH
RVISVEEVFELRLRAPDWVAMQTRQANLEGTGEIRLRRLIKEALRMRPDRILVGEVRQEE
HHCCHHHHHHHHCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHH
ALDLLIALNSGLPGMCSLHANSAREAVTKLCTLPLLAGENVTHSFVVPTVASSVDLVVHL
HHEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHCCEEEEEEE
SKEADGRRRVTEIAALPGRTEGDTVEVAQIFRTRGNMLVRADGFPPHPERYLRAGFDLPA
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCCCCCCE
LLAPSEHERAYAQIAVER
ECCCCHHHHHHHEEEECC
>Mature Secondary Structure
MNMEEERRFSVAAARGNWPDATSHVEGEVRELIRRRSLDPEREPVLIRQLVDAVLVDYEE
CCCCHHHHEEEEEECCCCCCCCHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHH
RALTSDLPPVADRDATARSVYDAVAGFGPLQRHLDDPAVEEIWINEPGRVFVARHGRSEL
HHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCHHHEEECCCCEEEEEECCCCHH
TTTILTQDQVQDLVERMLKSSGRRVDLSTPFVDAMLPDGSRLHVVIPDVTRVHWSVNIRK
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHCCCCCCEEEEEECCCEEEEEEEEHHH
FVLSAGSLDELVALGTITPNAAMFLEASVISGLNVIVAGGTQAGKTTMLNCLGSAIPARE
HHCCCCCHHHHHEEECCCCCHHHEEEHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCHHH
RVISVEEVFELRLRAPDWVAMQTRQANLEGTGEIRLRRLIKEALRMRPDRILVGEVRQEE
HHCCHHHHHHHHCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHH
ALDLLIALNSGLPGMCSLHANSAREAVTKLCTLPLLAGENVTHSFVVPTVASSVDLVVHL
HHEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHCCEEEEEEE
SKEADGRRRVTEIAALPGRTEGDTVEVAQIFRTRGNMLVRADGFPPHPERYLRAGFDLPA
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCCCCCCE
LLAPSEHERAYAQIAVER
ECCCCHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]