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The map label for this gene is ispB [C]

Identifier: 158317462

GI number: 158317462

Start: 6934911

End: 6935927

Strand: Reverse

Name: ispB [C]

Synonym: Franean1_5714

Alternate gene names: 158317462

Gene position: 6935927-6934911 (Counterclockwise)

Preceding gene: 158317463

Following gene: 158317461

Centisome position: 77.22

GC content: 74.63

Gene sequence:

>1017_bases
ATGACCACAACGGTTCCGGAAGCGATTGAGCGCGGGCGCGCCCTGGCGGTGCCCGCGCTGCGCGCCACGGTGGACCGGCT
CCACCCCCGGCTGCGCCACGTCGTCGCCTACCACCGCGGCTGGGTGGACGCGGCCGGTGAGCCGGTGCCCGGCAACGGCG
GCAAGCTCGTCCGGCCCGCGCTCGCCCTGCTCTCCGCCGAGGCGGCCGGCGCCCCGGCCGAGGTCGGCCTGCCGGCCGCG
GTCGCGGTCGAGCTGGTGCACGACTTCTCGCTGCTGCACGACGACCTGATGGACGGCGACCGCGAGCGCCGCCACCGGGC
CACCGCCTGGACGGTCTTCGGCGCCGACGGAGCCATCCTCGCCGGCGACGCACTGCTCGGCCTCGCCACCCAGGTCCTGC
TCGAGGTCGAGGGAGAGCCCGGCCGCCGCGCCGCGGTGATCCTCGGCGCCGGCGTCCAGGACCTGGTCCGCGGGCAGGCC
GAGGACCTCCTCTTCGAGAGCCGCTCGGACGTCTCGGTGGCCGAGACGCTGCACATGGAGGACGGCAAGACCGGTGCCCT
GCTGGCCTGCTCGGCCTCCCTCGGCGCGGTCCTCGCCGGCGCGCCCGACGAGGTGGTCGACGGCCTCGCCGAGTACGGCT
CGCGGCTGGGTACCGCCTTCCAGCTCATCGACGACCTGCTCGGCATCTGGGGCGACCCGGCGGTGACCGGCAAGCCCGTA
CTCAACGACCTGCGCTCGCGGAAGAAGTCGGTGCCGGTGGTCGTCGCGCTCGAGGCGGGCGGAGACGACGCCGACGAGCT
GCGGGCCTTCCTCACCGAGGAGGGTGAGGCCTCCGAGGACGACCTGCACCGCATCGCGGCCCTGGTCGAGCGGGCCGGTG
GCCGCGACTGGACCACGGCCGAGGCGGACCGCCAGCTCAAGGCGTGTGAAAACGCGCTGCGTTCGATCTCGATCCCGGCG
TCGGCCGAGGCGGAGCTGCTGGCGATGGCGCGGTTCGTGACCGAACGAGATCGCTGA

Upstream 100 bases:

>100_bases
CCGCGGCGACAGCGGCTGAACCGGCGGCGCCACACACAGCGACGCCACACACAGCGACGGCCCAGACAGAGACGTCTAAG
TCCACGGGGGGATTCCCGGT

Downstream 100 bases:

>100_bases
GGGGGAGCGAGGTGGCAGTACCCGCCCGCCGGTCGCCCGGCGGTCATCCGCACGACCAGTACATATACGACGAGGGGAGT
CGTCGATGAGCCTGACCTCG

Product: polyprenyl synthetase

Products: NA

Alternate protein names: GGPP synthase; GGPPSase; (2E,6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Farnesyltranstransferase; Geranylgeranyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MTTTVPEAIERGRALAVPALRATVDRLHPRLRHVVAYHRGWVDAAGEPVPGNGGKLVRPALALLSAEAAGAPAEVGLPAA
VAVELVHDFSLLHDDLMDGDRERRHRATAWTVFGADGAILAGDALLGLATQVLLEVEGEPGRRAAVILGAGVQDLVRGQA
EDLLFESRSDVSVAETLHMEDGKTGALLACSASLGAVLAGAPDEVVDGLAEYGSRLGTAFQLIDDLLGIWGDPAVTGKPV
LNDLRSRKKSVPVVVALEAGGDDADELRAFLTEEGEASEDDLHRIAALVERAGGRDWTTAEADRQLKACENALRSISIPA
SAEAELLAMARFVTERDR

Sequences:

>Translated_338_residues
MTTTVPEAIERGRALAVPALRATVDRLHPRLRHVVAYHRGWVDAAGEPVPGNGGKLVRPALALLSAEAAGAPAEVGLPAA
VAVELVHDFSLLHDDLMDGDRERRHRATAWTVFGADGAILAGDALLGLATQVLLEVEGEPGRRAAVILGAGVQDLVRGQA
EDLLFESRSDVSVAETLHMEDGKTGALLACSASLGAVLAGAPDEVVDGLAEYGSRLGTAFQLIDDLLGIWGDPAVTGKPV
LNDLRSRKKSVPVVVALEAGGDDADELRAFLTEEGEASEDDLHRIAALVERAGGRDWTTAEADRQLKACENALRSISIPA
SAEAELLAMARFVTERDR
>Mature_337_residues
TTTVPEAIERGRALAVPALRATVDRLHPRLRHVVAYHRGWVDAAGEPVPGNGGKLVRPALALLSAEAAGAPAEVGLPAAV
AVELVHDFSLLHDDLMDGDRERRHRATAWTVFGADGAILAGDALLGLATQVLLEVEGEPGRRAAVILGAGVQDLVRGQAE
DLLFESRSDVSVAETLHMEDGKTGALLACSASLGAVLAGAPDEVVDGLAEYGSRLGTAFQLIDDLLGIWGDPAVTGKPVL
NDLRSRKKSVPVVVALEAGGDDADELRAFLTEEGEASEDDLHRIAALVERAGGRDWTTAEADRQLKACENALRSISIPAS
AEAELLAMARFVTERDR

Specific function: Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate which is a precursor of the ether-linked lipids [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=297, Percent_Identity=30.6397306397306, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1789578, Length=297, Percent_Identity=28.956228956229, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786623, Length=243, Percent_Identity=33.7448559670782, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17505681, Length=237, Percent_Identity=31.2236286919831, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24651612, Length=269, Percent_Identity=27.5092936802974, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.1; =2.5.1.29; =2.5.1.10 [H]

Molecular weight: Translated: 35565; Mature: 35433

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTVPEAIERGRALAVPALRATVDRLHPRLRHVVAYHRGWVDAAGEPVPGNGGKLVRPA
CCCCCHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
LALLSAEAAGAPAEVGLPAAVAVELVHDFSLLHDDLMDGDRERRHRATAWTVFGADGAIL
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEEEECCCCCEE
AGDALLGLATQVLLEVEGEPGRRAAVILGAGVQDLVRGQAEDLLFESRSDVSVAETLHME
EHHHHHHHHHHHHHEECCCCCCEEEEEEECCHHHHHCCHHHHHHHCCCCCCHHHHHHCCC
DGKTGALLACSASLGAVLAGAPDEVVDGLAEYGSRLGTAFQLIDDLLGIWGDPAVTGKPV
CCCCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
LNDLRSRKKSVPVVVALEAGGDDADELRAFLTEEGEASEDDLHRIAALVERAGGRDWTTA
HHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCH
EADRQLKACENALRSISIPASAEAELLAMARFVTERDR
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTTVPEAIERGRALAVPALRATVDRLHPRLRHVVAYHRGWVDAAGEPVPGNGGKLVRPA
CCCCHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
LALLSAEAAGAPAEVGLPAAVAVELVHDFSLLHDDLMDGDRERRHRATAWTVFGADGAIL
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEEEECCCCCEE
AGDALLGLATQVLLEVEGEPGRRAAVILGAGVQDLVRGQAEDLLFESRSDVSVAETLHME
EHHHHHHHHHHHHHEECCCCCCEEEEEEECCHHHHHCCHHHHHHHCCCCCCHHHHHHCCC
DGKTGALLACSASLGAVLAGAPDEVVDGLAEYGSRLGTAFQLIDDLLGIWGDPAVTGKPV
CCCCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
LNDLRSRKKSVPVVVALEAGGDDADELRAFLTEEGEASEDDLHRIAALVERAGGRDWTTA
HHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCH
EADRQLKACENALRSISIPASAEAELLAMARFVTERDR
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]