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The map label for this gene is ptrB [C]

Identifier: 158317302

GI number: 158317302

Start: 6720875

End: 6722932

Strand: Direct

Name: ptrB [C]

Synonym: Franean1_5550

Alternate gene names: 158317302

Gene position: 6720875-6722932 (Clockwise)

Preceding gene: 158317301

Following gene: 158317303

Centisome position: 74.83

GC content: 67.25

Gene sequence:

>2058_bases
ATGGCCGACGATGATCCGCACTGCTGGCTTGAGGACGTCGCCGGGGAGGCCGCCCTCGCCTGGGTGAGGGAACACAACGC
CGAGACGTTCGCAGAGCTCACCCAGTCGGCCGAGTTCGCCGGGCTGCGCGCGGAGATCCGCGAGGTGCTCGACTCGGATG
ACCAGATTCCCTACCCGGTCACGAGGGGCGAGTACCTCTACAACTTCTGGCGCGACGCGGGTCATCCGCGTGGCCTGTGG
CGACGCACCTCGCTGGCGTCGTATCGCGATGCCAGCCCGGACTGGGAAGTCGTGCTCGACGTCGACGCGCTCGCCGCCCG
CGAGGGCGAGAACTGGGTGTGGCACGGCGCGCGGGTGCTGCGACCCGAATACCGGCTGGCTTTGGTCGAGCTGTCCCGTG
GCGGCGCGGACGCCTCCGTCATCCGTGAGTTCGACCTGGTCACGAAGTCCTTCGTGGAGGACGGCTTCTTCCTGCCCGAA
GCCAAAAGCTCGGTCGGTTGGCTCGATGAAGACCGGATCTATGTCGGTACCGATTTCGGCGAAGGATCGTTGACCACTTC
CGGCTACCCCCGCGTGATCCGAGAATGGCGGCGCGGCACTCCGCTCGACGATGCCGTCACAGTCTTCGAAGGCGAGGTGG
ATGACGTCTCGGTCGACGCCTACCACGACCCGACCGAGGGTTTCGTGCGCGACTTCGTCGACCGAAGTATCGACTTCTAC
CACACCGAGACGTTCCTGCGTATCCCGACGGGGCTGGTCCGCATCGACGTCCCGGACGACGCGCACACCTCGGTTCACCG
GGAGTGGCTGCTCATCACCACCCGGTCGGAGTGGGCTGTCGACGAGAAAGTCTATCCGGCGGGCGCCCTGCTCGCCGCCG
ACTTCGAAGCATTTCTCGCCGGACGACGAGAGCTCGAGCTCCTCTTCGAGCCGGACAAGCACACCTCGCTCGCGTACCAC
GCGTGGACTCGCAATCATCTCATCGTCGCCACTTTGCGGGACGTGAAAAGCCAGTTCACCGTGCTCACGCCGACAGCCTC
GGGCTGGACGAGGGCGCCGCTGGCCGACCTCCCGGACACCGGCTCCGCCTACATCAGCGACACCAGCCCCGACGTCGACG
ACGAATACTACCTCGGTGCCAGCGGCTACACCCAGCCAGCGACCTTGTACCGCGGCGAGATCGGCGTGGAGCCCGAGATC
CTCAAGCAGGCACCGGCGTTCTTCCCGACCGACGGCCGCACCGTCAGCCAGTATTTCGCCGTGTCCGACGACGGCACCCG
CGTCCCCTATTTCGTCGTCGGTACCGGAAAACCCGGGCCGACGCTGCTCTACGGGTACGGCGGCTTCGAGGTCTCGCTTA
CCCCCAGCTACAGCGGTACTGTCGGCCGGGCCTGGCTGGCGCGCGGCGGCACCTATGTCGTCGCCAACATCCGCGGTGGC
GGCGAATACGGCCCGGACTGGCATCAGTCAGCCATCCGCGAGAACCGGCTGCGTGCCTACGAGGATTTCGCCGCCGTGGC
CGGCGACCTCGTCGGCCGGGGGATCACGACGCCGGCCCAGCTCGGCATCGAGGGCGGTTCCAACGGCGGGCTGCTCATGG
GCGTCATGCTCACCCGCTACCCGGAGCTGTTCGGAGCCGTCGTCTGCTCGGTGCCGCTGCTTGACATGCGCCGCTACCAC
CAGCTGCTGGCCGGAGCCTCGTGGATGGCCGAGTACGGCGACCCTGACGATCCAGCCGACTGGGCGTTCATCAAGGAATA
CTCGCCGTACCAGAATGTCCGGCCCGGCCGTCGTTACCCGCCGACGTTCATCACCACGTCAACCCGCGACGACCGCGTCC
ACCCCGGGCACGCCCGCAAGATGGTCGCGCGGCTGCGCGAACTCGGCTACGCCATCCGCTACTACGAGAACATCGAAGGC
GGTCACAGCGGCGCCGCCGACAACGAGCAGCTTGCCCACAAATCAGCCCTGATCTACGAGTTCCTCTGGCGCACCCTCGG
CACCGGTGCGCTGTCAGACGGAGACCGTCCTGCCGCCGGGGCCGGTCGGACCGAGTGA

Upstream 100 bases:

>100_bases
CGACGGCGGCGGCCATGTCGGCAGCAGGAAGCCGAGCCCCTGATGATCGATAATCTTCTTGCCGACCGAACTGTCCCCCG
TGATCAGGCAGACTTGAGTG

Downstream 100 bases:

>100_bases
CCATCGAAGAAGATCTGCGTGCCGCGGTGGACGCCCGGTTCGCCGCCGCCGGTCCGGCGCCCTGGCCGGATCCGCATTGG
GACCAGGGACCGGCCGAGGA

Product: prolyl oligopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 685; Mature: 684

Protein sequence:

>685_residues
MADDDPHCWLEDVAGEAALAWVREHNAETFAELTQSAEFAGLRAEIREVLDSDDQIPYPVTRGEYLYNFWRDAGHPRGLW
RRTSLASYRDASPDWEVVLDVDALAAREGENWVWHGARVLRPEYRLALVELSRGGADASVIREFDLVTKSFVEDGFFLPE
AKSSVGWLDEDRIYVGTDFGEGSLTTSGYPRVIREWRRGTPLDDAVTVFEGEVDDVSVDAYHDPTEGFVRDFVDRSIDFY
HTETFLRIPTGLVRIDVPDDAHTSVHREWLLITTRSEWAVDEKVYPAGALLAADFEAFLAGRRELELLFEPDKHTSLAYH
AWTRNHLIVATLRDVKSQFTVLTPTASGWTRAPLADLPDTGSAYISDTSPDVDDEYYLGASGYTQPATLYRGEIGVEPEI
LKQAPAFFPTDGRTVSQYFAVSDDGTRVPYFVVGTGKPGPTLLYGYGGFEVSLTPSYSGTVGRAWLARGGTYVVANIRGG
GEYGPDWHQSAIRENRLRAYEDFAAVAGDLVGRGITTPAQLGIEGGSNGGLLMGVMLTRYPELFGAVVCSVPLLDMRRYH
QLLAGASWMAEYGDPDDPADWAFIKEYSPYQNVRPGRRYPPTFITTSTRDDRVHPGHARKMVARLRELGYAIRYYENIEG
GHSGAADNEQLAHKSALIYEFLWRTLGTGALSDGDRPAAGAGRTE

Sequences:

>Translated_685_residues
MADDDPHCWLEDVAGEAALAWVREHNAETFAELTQSAEFAGLRAEIREVLDSDDQIPYPVTRGEYLYNFWRDAGHPRGLW
RRTSLASYRDASPDWEVVLDVDALAAREGENWVWHGARVLRPEYRLALVELSRGGADASVIREFDLVTKSFVEDGFFLPE
AKSSVGWLDEDRIYVGTDFGEGSLTTSGYPRVIREWRRGTPLDDAVTVFEGEVDDVSVDAYHDPTEGFVRDFVDRSIDFY
HTETFLRIPTGLVRIDVPDDAHTSVHREWLLITTRSEWAVDEKVYPAGALLAADFEAFLAGRRELELLFEPDKHTSLAYH
AWTRNHLIVATLRDVKSQFTVLTPTASGWTRAPLADLPDTGSAYISDTSPDVDDEYYLGASGYTQPATLYRGEIGVEPEI
LKQAPAFFPTDGRTVSQYFAVSDDGTRVPYFVVGTGKPGPTLLYGYGGFEVSLTPSYSGTVGRAWLARGGTYVVANIRGG
GEYGPDWHQSAIRENRLRAYEDFAAVAGDLVGRGITTPAQLGIEGGSNGGLLMGVMLTRYPELFGAVVCSVPLLDMRRYH
QLLAGASWMAEYGDPDDPADWAFIKEYSPYQNVRPGRRYPPTFITTSTRDDRVHPGHARKMVARLRELGYAIRYYENIEG
GHSGAADNEQLAHKSALIYEFLWRTLGTGALSDGDRPAAGAGRTE
>Mature_684_residues
ADDDPHCWLEDVAGEAALAWVREHNAETFAELTQSAEFAGLRAEIREVLDSDDQIPYPVTRGEYLYNFWRDAGHPRGLWR
RTSLASYRDASPDWEVVLDVDALAAREGENWVWHGARVLRPEYRLALVELSRGGADASVIREFDLVTKSFVEDGFFLPEA
KSSVGWLDEDRIYVGTDFGEGSLTTSGYPRVIREWRRGTPLDDAVTVFEGEVDDVSVDAYHDPTEGFVRDFVDRSIDFYH
TETFLRIPTGLVRIDVPDDAHTSVHREWLLITTRSEWAVDEKVYPAGALLAADFEAFLAGRRELELLFEPDKHTSLAYHA
WTRNHLIVATLRDVKSQFTVLTPTASGWTRAPLADLPDTGSAYISDTSPDVDDEYYLGASGYTQPATLYRGEIGVEPEIL
KQAPAFFPTDGRTVSQYFAVSDDGTRVPYFVVGTGKPGPTLLYGYGGFEVSLTPSYSGTVGRAWLARGGTYVVANIRGGG
EYGPDWHQSAIRENRLRAYEDFAAVAGDLVGRGITTPAQLGIEGGSNGGLLMGVMLTRYPELFGAVVCSVPLLDMRRYHQ
LLAGASWMAEYGDPDDPADWAFIKEYSPYQNVRPGRRYPPTFITTSTRDDRVHPGHARKMVARLRELGYAIRYYENIEGG
HSGAADNEQLAHKSALIYEFLWRTLGTGALSDGDRPAAGAGRTE

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=367, Percent_Identity=32.1525885558583, Blast_Score=192, Evalue=9e-49,
Organism=Homo sapiens, GI284172420, Length=203, Percent_Identity=30.5418719211823, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI284172413, Length=203, Percent_Identity=30.5418719211823, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI70778815, Length=203, Percent_Identity=30.5418719211823, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI284172438, Length=203, Percent_Identity=30.5418719211823, Blast_Score=87, Evalue=7e-17,
Organism=Homo sapiens, GI284172431, Length=203, Percent_Identity=30.5418719211823, Blast_Score=87, Evalue=7e-17,
Organism=Homo sapiens, GI108860686, Length=203, Percent_Identity=30.5418719211823, Blast_Score=87, Evalue=7e-17,
Organism=Homo sapiens, GI108860692, Length=199, Percent_Identity=30.1507537688442, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1788150, Length=317, Percent_Identity=30.9148264984227, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI221510989, Length=325, Percent_Identity=33.5384615384615, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24583414, Length=410, Percent_Identity=30.7317073170732, Blast_Score=186, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 76135; Mature: 76004

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDDPHCWLEDVAGEAALAWVREHNAETFAELTQSAEFAGLRAEIREVLDSDDQIPYPV
CCCCCCCHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TRGEYLYNFWRDAGHPRGLWRRTSLASYRDASPDWEVVLDVDALAAREGENWVWHGARVL
CCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEEHHHHHCCCCCEEEECEEEE
RPEYRLALVELSRGGADASVIREFDLVTKSFVEDGFFLPEAKSSVGWLDEDRIYVGTDFG
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCC
EGSLTTSGYPRVIREWRRGTPLDDAVTVFEGEVDDVSVDAYHDPTEGFVRDFVDRSIDFY
CCCCCCCCCHHHHHHHHCCCCCCHHEEEEECCCCCEEEECCCCCHHHHHHHHHHCCCEEE
HTETFLRIPTGLVRIDVPDDAHTSVHREWLLITTRSEWAVDEKVYPAGALLAADFEAFLA
ECCEEEECCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHC
GRRELELLFEPDKHTSLAYHAWTRNHLIVATLRDVKSQFTVLTPTASGWTRAPLADLPDT
CCCEEEEEECCCCCCEEEEEEECCCCEEEEEHHHHHCCEEEEECCCCCCCCCCCCCCCCC
GSAYISDTSPDVDDEYYLGASGYTQPATLYRGEIGVEPEILKQAPAFFPTDGRTVSQYFA
CCEEECCCCCCCCCCEEECCCCCCCCCCEECCCCCCCHHHHHHCCCCCCCCCCEEEEEEE
VSDDGTRVPYFVVGTGKPGPTLLYGYGGFEVSLTPSYSGTVGRAWLARGGTYVVANIRGG
ECCCCCCCCEEEEECCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHCCCEEEEEEECCC
GEYGPDWHQSAIRENRLRAYEDFAAVAGDLVGRGITTPAQLGIEGGSNGGLLMGVMLTRY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEHHHHHHH
PELFGAVVCSVPLLDMRRYHQLLAGASWMAEYGDPDDPADWAFIKEYSPYQNVRPGRRYP
HHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCC
PTFITTSTRDDRVHPGHARKMVARLRELGYAIRYYENIEGGHSGAADNEQLAHKSALIYE
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHEEHHHCCCCCCCCCCCCHHHHHHHHHHHH
FLWRTLGTGALSDGDRPAAGAGRTE
HHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADDDPHCWLEDVAGEAALAWVREHNAETFAELTQSAEFAGLRAEIREVLDSDDQIPYPV
CCCCCCHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TRGEYLYNFWRDAGHPRGLWRRTSLASYRDASPDWEVVLDVDALAAREGENWVWHGARVL
CCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEEHHHHHCCCCCEEEECEEEE
RPEYRLALVELSRGGADASVIREFDLVTKSFVEDGFFLPEAKSSVGWLDEDRIYVGTDFG
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCC
EGSLTTSGYPRVIREWRRGTPLDDAVTVFEGEVDDVSVDAYHDPTEGFVRDFVDRSIDFY
CCCCCCCCCHHHHHHHHCCCCCCHHEEEEECCCCCEEEECCCCCHHHHHHHHHHCCCEEE
HTETFLRIPTGLVRIDVPDDAHTSVHREWLLITTRSEWAVDEKVYPAGALLAADFEAFLA
ECCEEEECCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHC
GRRELELLFEPDKHTSLAYHAWTRNHLIVATLRDVKSQFTVLTPTASGWTRAPLADLPDT
CCCEEEEEECCCCCCEEEEEEECCCCEEEEEHHHHHCCEEEEECCCCCCCCCCCCCCCCC
GSAYISDTSPDVDDEYYLGASGYTQPATLYRGEIGVEPEILKQAPAFFPTDGRTVSQYFA
CCEEECCCCCCCCCCEEECCCCCCCCCCEECCCCCCCHHHHHHCCCCCCCCCCEEEEEEE
VSDDGTRVPYFVVGTGKPGPTLLYGYGGFEVSLTPSYSGTVGRAWLARGGTYVVANIRGG
ECCCCCCCCEEEEECCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHCCCEEEEEEECCC
GEYGPDWHQSAIRENRLRAYEDFAAVAGDLVGRGITTPAQLGIEGGSNGGLLMGVMLTRY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEHHHHHHH
PELFGAVVCSVPLLDMRRYHQLLAGASWMAEYGDPDDPADWAFIKEYSPYQNVRPGRRYP
HHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCC
PTFITTSTRDDRVHPGHARKMVARLRELGYAIRYYENIEGGHSGAADNEQLAHKSALIYE
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHEEHHHCCCCCCCCCCCCHHHHHHHHHHHH
FLWRTLGTGALSDGDRPAAGAGRTE
HHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.47 {tosyl-Lys-methyl} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]