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The map label for this gene is scoF [H]

Identifier: 158317202

GI number: 158317202

Start: 6593028

End: 6593312

Strand: Direct

Name: scoF [H]

Synonym: Franean1_5449

Alternate gene names: 158317202

Gene position: 6593028-6593312 (Clockwise)

Preceding gene: 158317200

Following gene: 158317204

Centisome position: 73.4

GC content: 62.81

Gene sequence:

>285_bases
GTGATCAGAAGTTGGAGATCACCGCGCAGATTCGGGACACCACGTGGGTGTACCGCAGCAAGCGAGCGAGGAGCGACCGT
GTCAGAAGGTACTGTCAAGTGGTTCAACGCCGAGAAGGGCTTCGGCTTCATCTCAGTGGACGGCGGAGGGCCCGACGTCT
TCGCCCACTTCTCGTCCATCGTGGGTGACGGCTACAAGTCGCTCGAAGAGGGGCAGCGCGTGTCCTTCGACATCGAGCAG
GGACAGAAGGGGCCGCAGGCCGCCAAGGTGCAGCCCCTCAGCTAG

Upstream 100 bases:

>100_bases
CGCGATCGGCCGCAGGCGACACACTCGCGATCAAGGTGTACGGTGGGTCCGTTGCTCTTCTATTTGGGTGCCGCCGGTCA
CCTTCAGGATGGCATCACCG

Downstream 100 bases:

>100_bases
CACAAGTAGATAGAAGCCAGCTCGTCTTGGGGCTAGAACCTCAGCTTTTGTAGGGGTCCGACAAGCTCGGTCGCGTAGCT
GCGTGGCAACCGGCCCACGT

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 94; Mature: 94

Protein sequence:

>94_residues
MIRSWRSPRRFGTPRGCTAASERGATVSEGTVKWFNAEKGFGFISVDGGGPDVFAHFSSIVGDGYKSLEEGQRVSFDIEQ
GQKGPQAAKVQPLS

Sequences:

>Translated_94_residues
MIRSWRSPRRFGTPRGCTAASERGATVSEGTVKWFNAEKGFGFISVDGGGPDVFAHFSSIVGDGYKSLEEGQRVSFDIEQ
GQKGPQAAKVQPLS
>Mature_94_residues
MIRSWRSPRRFGTPRGCTAASERGATVSEGTVKWFNAEKGFGFISVDGGGPDVFAHFSSIVGDGYKSLEEGQRVSFDIEQ
GQKGPQAAKVQPLS

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=67.1875, Blast_Score=91, Evalue=2e-20,
Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=55.2238805970149, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=57.8125, Blast_Score=83, Evalue=3e-18,
Organism=Escherichia coli, GI1787839, Length=61, Percent_Identity=63.9344262295082, Blast_Score=82, Evalue=5e-18,
Organism=Escherichia coli, GI1786841, Length=65, Percent_Identity=58.4615384615385, Blast_Score=81, Evalue=2e-17,
Organism=Escherichia coli, GI1788126, Length=65, Percent_Identity=56.9230769230769, Blast_Score=79, Evalue=4e-17,
Organism=Escherichia coli, GI1787834, Length=64, Percent_Identity=56.25, Blast_Score=74, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 10109; Mature: 10109

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRSWRSPRRFGTPRGCTAASERGATVSEGTVKWFNAEKGFGFISVDGGGPDVFAHFSSI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHH
VGDGYKSLEEGQRVSFDIEQGQKGPQAAKVQPLS
HCCCHHHHHCCCEEEEECCCCCCCCCCCEECCCC
>Mature Secondary Structure
MIRSWRSPRRFGTPRGCTAASERGATVSEGTVKWFNAEKGFGFISVDGGGPDVFAHFSSI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHH
VGDGYKSLEEGQRVSFDIEQGQKGPQAAKVQPLS
HCCCHHHHHCCCEEEEECCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]