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The map label for this gene is tgs1 [H]

Identifier: 158317164

GI number: 158317164

Start: 6527763

End: 6529334

Strand: Direct

Name: tgs1 [H]

Synonym: Franean1_5411

Alternate gene names: 158317164

Gene position: 6527763-6529334 (Clockwise)

Preceding gene: 158317163

Following gene: 158317166

Centisome position: 72.68

GC content: 70.8

Gene sequence:

>1572_bases
ATGACCGCTCGGCCGCTTACTCCGATGGACGCGCTCATACTCGGATACCAGCGAGCATTTCCGAAGACGCCGCTCGCGGT
CGGTTGCCTGTTGGTCGCCGACGGCCCCGTGCCAGGAGTCCAGGCGTTGCGCGAGCTCGTCGCGGACCGGGCACACGACT
TTCCTCCACTGGCCCACCGGATCGCCTCGGCCGGGCGTGGCCGGCCGGTCTGGGTGGCGGACGCCGATTTCGACCCGGCC
CGGCACGTTCACGAGTACCGGCTCCCCGCGGAGTCGGGGCTCGCCGGACTGCGCGAGGCGGTGGGGCGGCTGTCAGCGGT
CGAGATCTCGTTGGACGCGCCGCCGTGGCAGCTCTGGTTGCTCCACGGCCTCCGCCGCAACGGCTTCACCCTGCTGTACC
GCGCCAGCCACGTGTGGACAGACGGGACGGCACTGAACCTGGTGCTGGAAAAGCTGTTCGGCCTGTCCGACCCAGGATCG
GAGCGCGCACCTCTGCGGGTGTCGCCGGATCGCCGGCCCGGCCCCCGAACCGTCTGTCGGGCCGCCACTCACTCACTCGG
ATGGCTTACCCGGACGTCCACGATCGGTCCGCTCTCGGCGGCACCCACGGGATACCCGCACCACACCTGGCTGGAGGTGG
ACCTGTCCAGGCTACGGGCGATCAGCCGCGCCTACGAGGTCACCGTGAACGACATCTTCCTGGCCGCGCTCACCGGCGCT
CTGCGTGCCTGGCCGCGCCCCGGCTCCGATCGGCCCGGCCAGCGCCGGGGCCAGATGCACGCCGCGATGCCGGTCAGTAC
CCGCCGGGCAGCCCAGCGGGACCACATGAGCAACTACCTGACCACCGTACGGATCGCGCTGCCTTACGGTGAGTCGTTGA
TCCACCGGCGTGTGGAGGCGATCCACCGGCAGACCGTCCGACACAAGCGGGGCGGGACTCCGGGCGTCGCGGAACATCTC
TTCCTCTGGGCGATTCCCGAACCGTTGCGACCGGCCGTGCTGTCCACCGGGATCATGTCCCACGTCTTCGCGCTGACCGC
ATCCAACCCGGGCGGCCTGACCGGCCCGCTGGAAATCCTCGGCCGACCAGTCACCGCCGCCGTGCCCACCCCTCCGCTCC
CCGCAGGCCAACGCCTGGCAGTCCTGCTCGGCGGGCTGGACGGGCAGGCGTGCATCGGTTTCACGATGGACGGGTCGGTG
CGGGACGGGGCGCGACTGCCGGAACTCGTCGAGGCCGAACTGGACGCGCTCGAGGCAGCGGCCGGCCTCCGCCACGGCCC
GGCCCGGAGCCCGGCCGAGACCACGCATTCGGCACGGCCGCATCCGGTCGCCATGACCGGTATCGGCACCAGCGCTATCG
AGGCGGCGGGTCTGGTGGGGCGCGGTGCTTATCACCTTCGTCGCTGGCTCACACGGGGCCAGCGGACCGACACGCCTGGC
ACGGAACTGGTTCAGGGCACCAGCGACGCGGCAGGTCGAACGCCCTACAGTCAACTCTGCTCCCGACGGCTCCAGCAACC
CCGGCCAGCCGGTGCTTCGCCAGCAGAGGAGAGGCGAGTACTACATTCCTAG

Upstream 100 bases:

>100_bases
CAGCATGGCGTTCCGGACGTAACCGGTTTTCCAGTCCATCCAGGGAGCCAGTTGTGACAATTGCGCGGCGACTCGTCGAT
GTTAACCGGGGCGCCAAGCT

Downstream 100 bases:

>100_bases
ATCTTGGCTGGTTGGTTCGGGTTCAGGTGCGTAGAGCGAGGGGGATCGGGTCGCCGCGGCGCCGGTAGTGGCTGAGGAGC
GCTCGGGTCTGGTGGGCCAT

Product: hypothetical protein

Products: NA

Alternate protein names: TGS1; Probable triacylglycerol synthase tgs1 [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MTARPLTPMDALILGYQRAFPKTPLAVGCLLVADGPVPGVQALRELVADRAHDFPPLAHRIASAGRGRPVWVADADFDPA
RHVHEYRLPAESGLAGLREAVGRLSAVEISLDAPPWQLWLLHGLRRNGFTLLYRASHVWTDGTALNLVLEKLFGLSDPGS
ERAPLRVSPDRRPGPRTVCRAATHSLGWLTRTSTIGPLSAAPTGYPHHTWLEVDLSRLRAISRAYEVTVNDIFLAALTGA
LRAWPRPGSDRPGQRRGQMHAAMPVSTRRAAQRDHMSNYLTTVRIALPYGESLIHRRVEAIHRQTVRHKRGGTPGVAEHL
FLWAIPEPLRPAVLSTGIMSHVFALTASNPGGLTGPLEILGRPVTAAVPTPPLPAGQRLAVLLGGLDGQACIGFTMDGSV
RDGARLPELVEAELDALEAAAGLRHGPARSPAETTHSARPHPVAMTGIGTSAIEAAGLVGRGAYHLRRWLTRGQRTDTPG
TELVQGTSDAAGRTPYSQLCSRRLQQPRPAGASPAEERRVLHS

Sequences:

>Translated_523_residues
MTARPLTPMDALILGYQRAFPKTPLAVGCLLVADGPVPGVQALRELVADRAHDFPPLAHRIASAGRGRPVWVADADFDPA
RHVHEYRLPAESGLAGLREAVGRLSAVEISLDAPPWQLWLLHGLRRNGFTLLYRASHVWTDGTALNLVLEKLFGLSDPGS
ERAPLRVSPDRRPGPRTVCRAATHSLGWLTRTSTIGPLSAAPTGYPHHTWLEVDLSRLRAISRAYEVTVNDIFLAALTGA
LRAWPRPGSDRPGQRRGQMHAAMPVSTRRAAQRDHMSNYLTTVRIALPYGESLIHRRVEAIHRQTVRHKRGGTPGVAEHL
FLWAIPEPLRPAVLSTGIMSHVFALTASNPGGLTGPLEILGRPVTAAVPTPPLPAGQRLAVLLGGLDGQACIGFTMDGSV
RDGARLPELVEAELDALEAAAGLRHGPARSPAETTHSARPHPVAMTGIGTSAIEAAGLVGRGAYHLRRWLTRGQRTDTPG
TELVQGTSDAAGRTPYSQLCSRRLQQPRPAGASPAEERRVLHS
>Mature_522_residues
TARPLTPMDALILGYQRAFPKTPLAVGCLLVADGPVPGVQALRELVADRAHDFPPLAHRIASAGRGRPVWVADADFDPAR
HVHEYRLPAESGLAGLREAVGRLSAVEISLDAPPWQLWLLHGLRRNGFTLLYRASHVWTDGTALNLVLEKLFGLSDPGSE
RAPLRVSPDRRPGPRTVCRAATHSLGWLTRTSTIGPLSAAPTGYPHHTWLEVDLSRLRAISRAYEVTVNDIFLAALTGAL
RAWPRPGSDRPGQRRGQMHAAMPVSTRRAAQRDHMSNYLTTVRIALPYGESLIHRRVEAIHRQTVRHKRGGTPGVAEHLF
LWAIPEPLRPAVLSTGIMSHVFALTASNPGGLTGPLEILGRPVTAAVPTPPLPAGQRLAVLLGGLDGQACIGFTMDGSVR
DGARLPELVEAELDALEAAAGLRHGPARSPAETTHSARPHPVAMTGIGTSAIEAAGLVGRGAYHLRRWLTRGQRTDTPGT
ELVQGTSDAAGRTPYSQLCSRRLQQPRPAGASPAEERRVLHS

Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Prefers C(26:0)-CoA over C(18:1)-CoA. TG synthesis activity increases in M.tuberculosis upon oxygen depletion and NO

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 56310; Mature: 56179

Theoretical pI: Translated: 10.92; Mature: 10.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARPLTPMDALILGYQRAFPKTPLAVGCLLVADGPVPGVQALRELVADRAHDFPPLAHR
CCCCCCCHHHHHHHHHHHHCCCCCHHEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHH
IASAGRGRPVWVADADFDPARHVHEYRLPAESGLAGLREAVGRLSAVEISLDAPPWQLWL
HHHCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHH
LHGLRRNGFTLLYRASHVWTDGTALNLVLEKLFGLSDPGSERAPLRVSPDRRPGPRTVCR
HHHHCCCCEEEEEEECEEECCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHH
AATHSLGWLTRTSTIGPLSAAPTGYPHHTWLEVDLSRLRAISRAYEVTVNDIFLAALTGA
HHHHHHHHEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRAWPRPGSDRPGQRRGQMHAAMPVSTRRAAQRDHMSNYLTTVRIALPYGESLIHRRVEA
HHCCCCCCCCCCCHHHCCEEECCCCHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHH
IHRQTVRHKRGGTPGVAEHLFLWAIPEPLRPAVLSTGIMSHVFALTASNPGGLTGPLEIL
HHHHHHHHHCCCCCCHHHHEEEEECCCCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHH
GRPVTAAVPTPPLPAGQRLAVLLGGLDGQACIGFTMDGSVRDGARLPELVEAELDALEAA
CCCCEECCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
AGLRHGPARSPAETTHSARPHPVAMTGIGTSAIEAAGLVGRGAYHLRRWLTRGQRTDTPG
HHCCCCCCCCCHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
TELVQGTSDAAGRTPYSQLCSRRLQQPRPAGASPAEERRVLHS
HHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
TARPLTPMDALILGYQRAFPKTPLAVGCLLVADGPVPGVQALRELVADRAHDFPPLAHR
CCCCCCHHHHHHHHHHHHCCCCCHHEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHH
IASAGRGRPVWVADADFDPARHVHEYRLPAESGLAGLREAVGRLSAVEISLDAPPWQLWL
HHHCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHH
LHGLRRNGFTLLYRASHVWTDGTALNLVLEKLFGLSDPGSERAPLRVSPDRRPGPRTVCR
HHHHCCCCEEEEEEECEEECCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHH
AATHSLGWLTRTSTIGPLSAAPTGYPHHTWLEVDLSRLRAISRAYEVTVNDIFLAALTGA
HHHHHHHHEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRAWPRPGSDRPGQRRGQMHAAMPVSTRRAAQRDHMSNYLTTVRIALPYGESLIHRRVEA
HHCCCCCCCCCCCHHHCCEEECCCCHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHH
IHRQTVRHKRGGTPGVAEHLFLWAIPEPLRPAVLSTGIMSHVFALTASNPGGLTGPLEIL
HHHHHHHHHCCCCCCHHHHEEEEECCCCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHH
GRPVTAAVPTPPLPAGQRLAVLLGGLDGQACIGFTMDGSVRDGARLPELVEAELDALEAA
CCCCEECCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
AGLRHGPARSPAETTHSARPHPVAMTGIGTSAIEAAGLVGRGAYHLRRWLTRGQRTDTPG
HHCCCCCCCCCHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
TELVQGTSDAAGRTPYSQLCSRRLQQPRPAGASPAEERRVLHS
HHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]