Click here to switch to the map view.

The map label for this gene is gshB [H]

Identifier: 158317159

GI number: 158317159

Start: 6521158

End: 6522129

Strand: Reverse

Name: gshB [H]

Synonym: Franean1_5406

Alternate gene names: 158317159

Gene position: 6522129-6521158 (Counterclockwise)

Preceding gene: 158317160

Following gene: 158317158

Centisome position: 72.61

GC content: 70.78

Gene sequence:

>972_bases
GTGAAAGTCGTGTTCGTCACCGATCCGGTGGAGGCGCTCGACCCGTCCATCGACACCAGCGTCGGCCTCATGCACGCGGC
GCAGGACCGTGACCTGGAGGTGTGGGTCACCGAAGCGCTCCTGCTCGAAGCCGTGAGCGGTCGGGCCCGAGCCCTGGCCC
GCCGGCTGCGGCTCGCTCCGTCCCGACCAGACGGCGGTCACCGGTGGACGGTCGCCGACACCTGGTTCACCGCCAGCGAG
CCGCGGCACGTCTGGCTCGACGACGCGGCCGCCGTCTTCATGCGCACCGACCCGCCGCTCGACCGGACGTACCTCACCGC
GACCCTGATCCTGGACCTCGTCGATCCGGCCCGGACCGTCATGGTCAACGATCCGCGCGGCTTGCGGATCTGCGGCGAGC
ACATGCTGCCGCTGCAGTTCGCGGACCTGGCACCACCGACCATCGTCACTGCGGACCCGCGTGCCATCCGGTCGTTCCTC
GCCGAGCACGGTGTAGTCGTGGTCAAGCCGGTGGACGGCTTCGGCGGTCGCGGCGTCCTGCGGCTGCACCGCCGTGATCC
CAATCTCGCGTCGCTGCTGGAGACGGCCACGCACGACGGCCGCCGAGCCGTTGTCGCGCAGCCGTTCCTCCGGGAGGTCT
CGGCCGGCAACAAGCGCATCTTCGTGGTGGCGGGGGAACCGGTGGGCGCGGTCTGCCGCTACCCGGCGGACGGCGACTTC
CGGATCGGCCAGCCGACCGCCGAGGCGCCACTCACCGACCGCGACCGCGAGATCTGCGCCCGCCTGGCACCCAGCCTGCG
GCGGCACGGCATCCACCTGGCCGGCCTGGACGTGATCGGGCCGCACCTCATCGAAGTGAACGTGACCAGCGTCGGAGCGC
TGCGAAAGGCCGACGCGCTGCTCGGCTGGAGCCTCTGCGCCGACCTCGTCGACAGCGTTCTGAAAACGCACGACCAAAGG
GATTTCACATGA

Upstream 100 bases:

>100_bases
TCGTCAGCCCCCTCGGTGCCCCTCCGCAGTTCGCCGGCTTCGCCACCGCCGACCTCGACGTCCGCTGAGCGCGCACAGAG
AACCTCGGGGGAGGGACGTC

Downstream 100 bases:

>100_bases
CCACCGCCATCATCTGCACGGCGATTCTGAGCGCGATGGTGTTCCTGCTCGGATTCAACGTCTCGCGCGCACGCGGCCTG
GCCTCCAAGGCCGGCGGCTC

Product: glutathione synthase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKVVFVTDPVEALDPSIDTSVGLMHAAQDRDLEVWVTEALLLEAVSGRARALARRLRLAPSRPDGGHRWTVADTWFTASE
PRHVWLDDAAAVFMRTDPPLDRTYLTATLILDLVDPARTVMVNDPRGLRICGEHMLPLQFADLAPPTIVTADPRAIRSFL
AEHGVVVVKPVDGFGGRGVLRLHRRDPNLASLLETATHDGRRAVVAQPFLREVSAGNKRIFVVAGEPVGAVCRYPADGDF
RIGQPTAEAPLTDRDREICARLAPSLRRHGIHLAGLDVIGPHLIEVNVTSVGALRKADALLGWSLCADLVDSVLKTHDQR
DFT

Sequences:

>Translated_323_residues
MKVVFVTDPVEALDPSIDTSVGLMHAAQDRDLEVWVTEALLLEAVSGRARALARRLRLAPSRPDGGHRWTVADTWFTASE
PRHVWLDDAAAVFMRTDPPLDRTYLTATLILDLVDPARTVMVNDPRGLRICGEHMLPLQFADLAPPTIVTADPRAIRSFL
AEHGVVVVKPVDGFGGRGVLRLHRRDPNLASLLETATHDGRRAVVAQPFLREVSAGNKRIFVVAGEPVGAVCRYPADGDF
RIGQPTAEAPLTDRDREICARLAPSLRRHGIHLAGLDVIGPHLIEVNVTSVGALRKADALLGWSLCADLVDSVLKTHDQR
DFT
>Mature_323_residues
MKVVFVTDPVEALDPSIDTSVGLMHAAQDRDLEVWVTEALLLEAVSGRARALARRLRLAPSRPDGGHRWTVADTWFTASE
PRHVWLDDAAAVFMRTDPPLDRTYLTATLILDLVDPARTVMVNDPRGLRICGEHMLPLQFADLAPPTIVTADPRAIRSFL
AEHGVVVVKPVDGFGGRGVLRLHRRDPNLASLLETATHDGRRAVVAQPFLREVSAGNKRIFVVAGEPVGAVCRYPADGDF
RIGQPTAEAPLTDRDREICARLAPSLRRHGIHLAGLDVIGPHLIEVNVTSVGALRKADALLGWSLCADLVDSVLKTHDQR
DFT

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=318, Percent_Identity=33.3333333333333, Blast_Score=188, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35308; Mature: 35308

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVFVTDPVEALDPSIDTSVGLMHAAQDRDLEVWVTEALLLEAVSGRARALARRLRLAP
CEEEEEECCHHHHCCCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHCCHHHHHHHHHHCCC
SRPDGGHRWTVADTWFTASEPRHVWLDDAAAVFMRTDPPLDRTYLTATLILDLVDPARTV
CCCCCCCEEEEEEEEEECCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEE
MVNDPRGLRICGEHMLPLQFADLAPPTIVTADPRAIRSFLAEHGVVVVKPVDGFGGRGVL
EEECCCCCEEECCCCCCEEECCCCCCEEEECCHHHHHHHHHHCCEEEEEECCCCCCCCEE
RLHRRDPNLASLLETATHDGRRAVVAQPFLREVSAGNKRIFVVAGEPVGAVCRYPADGDF
EEECCCCCHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCCCE
RIGQPTAEAPLTDRDREICARLAPSLRRHGIHLAGLDVIGPHLIEVNVTSVGALRKADAL
ECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHCCCEEEEEEECCCCHHHHHHHH
LGWSLCADLVDSVLKTHDQRDFT
HHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVVFVTDPVEALDPSIDTSVGLMHAAQDRDLEVWVTEALLLEAVSGRARALARRLRLAP
CEEEEEECCHHHHCCCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHCCHHHHHHHHHHCCC
SRPDGGHRWTVADTWFTASEPRHVWLDDAAAVFMRTDPPLDRTYLTATLILDLVDPARTV
CCCCCCCEEEEEEEEEECCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEE
MVNDPRGLRICGEHMLPLQFADLAPPTIVTADPRAIRSFLAEHGVVVVKPVDGFGGRGVL
EEECCCCCEEECCCCCCEEECCCCCCEEEECCHHHHHHHHHHCCEEEEEECCCCCCCCEE
RLHRRDPNLASLLETATHDGRRAVVAQPFLREVSAGNKRIFVVAGEPVGAVCRYPADGDF
EEECCCCCHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCCCE
RIGQPTAEAPLTDRDREICARLAPSLRRHGIHLAGLDVIGPHLIEVNVTSVGALRKADAL
ECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHCCCEEEEEEECCCCHHHHHHHH
LGWSLCADLVDSVLKTHDQRDFT
HHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308172 [H]