Click here to switch to the map view.

The map label for this gene is murD [H]

Identifier: 158316857

GI number: 158316857

Start: 6104726

End: 6106306

Strand: Reverse

Name: murD [H]

Synonym: Franean1_5100

Alternate gene names: 158316857

Gene position: 6106306-6104726 (Counterclockwise)

Preceding gene: 158316858

Following gene: 158316856

Centisome position: 67.98

GC content: 77.67

Gene sequence:

>1581_bases
ATGAGTACTTCCGCCACACCGGTGCTCGCGGGCGCGGTGCTCGCCGGCGCTCAGGTCACGGTCGTCGGAGTGGGCGTCTC
CGGCGCCGCGGCGGCCGGTGCGCTGGCGGCCCGCGGCGCCCGGGTGGTCGTGGTGGACGCGGCCGACGGCCCGGGCCAGC
GCGCCGCCGCCGAGCGGCTGCGGGCGGAGGGAATCGAGGTCCGGCTCGGCGGCCTGCCGGCCGAGGCCGGGGCGGCGTCG
CTCGTCGTGACCTCGCCCGGTGTCCCACCGCACACGCCGCTGCTGCGGGCGGCGGAGGCCGCCGGGGTACCGGTTTGGGG
CGAGGTGGAGCTCGCCTGGCGGTGGCGCGGGCTGTCCCGGTGGCTCGCGGTCACCGGCACGAACGGCAAGACCACCACGA
CCGAGATGCTCGGCGCGATGCTCGTCGCCGGGGGGCGGCGCGCCACCACAGCCGGCAACATCGGCACGCCCATCGTGGCG
GCCGTCGAGACCCGGCCGCCCTACGACGCGCTCGCCGTCGAGCTGTCCAGCTTCCAGCTGCACTACACGTCCACCGTCGC
CCCGCGCGCGGCGGTGATCCTCAACGTCGCGCCGGACCATCTCGACTGGCACGGCGGAGCCGACGCCTACGCGGCGGCGA
AGGCGCGGATCTGGGCGGCCCCGCACACCGTCGCCGTCGGCAACGTGGACGACCCCGTGAGCCTGGAGCTGCTGTCGCGG
GCGCCGGGCCGGCGGGTCACCTTCGGGCTGGACCCGAACGCCACCCCACGGCCCGACCTGACCGTCGTGAACGGGTACCT
GGTCGACCGGGTCTTCTCCGGCGGGCGGCTGATCGCCGTGGCCGATCTCGGCGCGGCCGGCCGCTCACCCGGGCGGCGCC
CGCCCGCCACCGGCATCACCGGCGGCGGGCCGGCCGGCGGCAGGGTCGGCGGCGCCGTGGCGGGTGGCGCCCGGGCCGGC
GCGGCCGGGACCGGCGACGGGCCGGGCCCGTTCGGCGGCGGCCAGTTCCGGGACGACCGCTTCCCCGCCTTCGCCACCGG
CGCTGAGCTGGGATCGCACAACATCGCCAACGCGCTGGCCGCCGCCGCGCTGGCCCGCGCCGACGGGGTGGACCCGGCGG
CCATCGGCGCGGCGCTCGCGTCGTTCCGGCCCGGCGCGCACCGCAACGTCACCGTCGGCCGGCGCGGCGGCGTCCGCTAC
GTCGACGACAGCAAGGCCACCAACCCGCATGCCGCCGCCGCGTCGCTGCGCGCCTACCCGTCGGTGGTGTGGATCGCGGG
CGGTCTGAACAAGGGCCTTGAGTTCGACGATCTCGTTGTCTCGGCCCGTCGCGGGCTGCGCGCGGTGGTCCTGATCGGCC
AGTGCGCCGACGAGGTGGCCGCGGCTCTCGCCCGACACGCCCCCGATGTCCCCGTGGAACGGGCCGCGGGCATGGACGAT
GCGGTGGACGCCGCCGCGAAGCTCGCGGCACCGGGCGACACCGTGCTGCTGGCGCCCGCGGCGGCGTCGATGGACATGTT
CCGTGACTACGCGGAGCGCGGCGACCTGTTCGCCGCGGCCGTGCGAGCGCGGGGAGGCTGA

Upstream 100 bases:

>100_bases
CGACGGTGATCATCCGGTTCTGGATCGTCTCCGGCCTGGCCGTAGCGTTCGGCCTGGGCCTGTTCTACGCCGAGTACCTT
TCCCACGGGGGCGGTTGACC

Downstream 100 bases:

>100_bases
CCGCCGGCGCTCTCCCTCCCAGCGCGGCACCGGAGAGGGGGAGCGTGAGTGAGCGCATGACGGGTGCGCGAACCACCGGA
ACGGGGACGGCCGTCGCCGG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSTSATPVLAGAVLAGAQVTVVGVGVSGAAAAGALAARGARVVVVDAADGPGQRAAAERLRAEGIEVRLGGLPAEAGAAS
LVVTSPGVPPHTPLLRAAEAAGVPVWGEVELAWRWRGLSRWLAVTGTNGKTTTTEMLGAMLVAGGRRATTAGNIGTPIVA
AVETRPPYDALAVELSSFQLHYTSTVAPRAAVILNVAPDHLDWHGGADAYAAAKARIWAAPHTVAVGNVDDPVSLELLSR
APGRRVTFGLDPNATPRPDLTVVNGYLVDRVFSGGRLIAVADLGAAGRSPGRRPPATGITGGGPAGGRVGGAVAGGARAG
AAGTGDGPGPFGGGQFRDDRFPAFATGAELGSHNIANALAAAALARADGVDPAAIGAALASFRPGAHRNVTVGRRGGVRY
VDDSKATNPHAAAASLRAYPSVVWIAGGLNKGLEFDDLVVSARRGLRAVVLIGQCADEVAAALARHAPDVPVERAAGMDD
AVDAAAKLAAPGDTVLLAPAAASMDMFRDYAERGDLFAAAVRARGG

Sequences:

>Translated_526_residues
MSTSATPVLAGAVLAGAQVTVVGVGVSGAAAAGALAARGARVVVVDAADGPGQRAAAERLRAEGIEVRLGGLPAEAGAAS
LVVTSPGVPPHTPLLRAAEAAGVPVWGEVELAWRWRGLSRWLAVTGTNGKTTTTEMLGAMLVAGGRRATTAGNIGTPIVA
AVETRPPYDALAVELSSFQLHYTSTVAPRAAVILNVAPDHLDWHGGADAYAAAKARIWAAPHTVAVGNVDDPVSLELLSR
APGRRVTFGLDPNATPRPDLTVVNGYLVDRVFSGGRLIAVADLGAAGRSPGRRPPATGITGGGPAGGRVGGAVAGGARAG
AAGTGDGPGPFGGGQFRDDRFPAFATGAELGSHNIANALAAAALARADGVDPAAIGAALASFRPGAHRNVTVGRRGGVRY
VDDSKATNPHAAAASLRAYPSVVWIAGGLNKGLEFDDLVVSARRGLRAVVLIGQCADEVAAALARHAPDVPVERAAGMDD
AVDAAAKLAAPGDTVLLAPAAASMDMFRDYAERGDLFAAAVRARGG
>Mature_525_residues
STSATPVLAGAVLAGAQVTVVGVGVSGAAAAGALAARGARVVVVDAADGPGQRAAAERLRAEGIEVRLGGLPAEAGAASL
VVTSPGVPPHTPLLRAAEAAGVPVWGEVELAWRWRGLSRWLAVTGTNGKTTTTEMLGAMLVAGGRRATTAGNIGTPIVAA
VETRPPYDALAVELSSFQLHYTSTVAPRAAVILNVAPDHLDWHGGADAYAAAKARIWAAPHTVAVGNVDDPVSLELLSRA
PGRRVTFGLDPNATPRPDLTVVNGYLVDRVFSGGRLIAVADLGAAGRSPGRRPPATGITGGGPAGGRVGGAVAGGARAGA
AGTGDGPGPFGGGQFRDDRFPAFATGAELGSHNIANALAAAALARADGVDPAAIGAALASFRPGAHRNVTVGRRGGVRYV
DDSKATNPHAAAASLRAYPSVVWIAGGLNKGLEFDDLVVSARRGLRAVVLIGQCADEVAAALARHAPDVPVERAAGMDDA
VDAAAKLAAPGDTVLLAPAAASMDMFRDYAERGDLFAAAVRARGG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=238, Percent_Identity=36.1344537815126, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 52542; Mature: 52411

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSATPVLAGAVLAGAQVTVVGVGVSGAAAAGALAARGARVVVVDAADGPGQRAAAERL
CCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
RAEGIEVRLGGLPAEAGAASLVVTSPGVPPHTPLLRAAEAAGVPVWGEVELAWRWRGLSR
HHCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCEECEEEEEEEECCCEE
WLAVTGTNGKTTTTEMLGAMLVAGGRRATTAGNIGTPIVAAVETRPPYDALAVELSSFQL
EEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHEEEEEEEEE
HYTSTVAPRAAVILNVAPDHLDWHGGADAYAAAKARIWAAPHTVAVGNVDDPVSLELLSR
EEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHEEEEECCCEEEECCCCCCCHHHHHHC
APGRRVTFGLDPNATPRPDLTVVNGYLVDRVFSGGRLIAVADLGAAGRSPGRRPPATGIT
CCCCEEEEECCCCCCCCCCEEEECHHEEEHHCCCCCEEEEEECCCCCCCCCCCCCCCCCC
GGGPAGGRVGGAVAGGARAGAAGTGDGPGPFGGGQFRDDRFPAFATGAELGSHNIANALA
CCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHCCCHHHHHHH
AAALARADGVDPAAIGAALASFRPGAHRNVTVGRRGGVRYVDDSKATNPHAAAASLRAYP
HHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHHCCC
SVVWIAGGLNKGLEFDDLVVSARRGLRAVVLIGQCADEVAAALARHAPDVPVERAAGMDD
CEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCCHHHHCCCCH
AVDAAAKLAAPGDTVLLAPAAASMDMFRDYAERGDLFAAAVRARGG
HHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCEEEEHHHCCCC
>Mature Secondary Structure 
STSATPVLAGAVLAGAQVTVVGVGVSGAAAAGALAARGARVVVVDAADGPGQRAAAERL
CCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
RAEGIEVRLGGLPAEAGAASLVVTSPGVPPHTPLLRAAEAAGVPVWGEVELAWRWRGLSR
HHCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCEECEEEEEEEECCCEE
WLAVTGTNGKTTTTEMLGAMLVAGGRRATTAGNIGTPIVAAVETRPPYDALAVELSSFQL
EEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHEEEEEEEEE
HYTSTVAPRAAVILNVAPDHLDWHGGADAYAAAKARIWAAPHTVAVGNVDDPVSLELLSR
EEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHEEEEECCCEEEECCCCCCCHHHHHHC
APGRRVTFGLDPNATPRPDLTVVNGYLVDRVFSGGRLIAVADLGAAGRSPGRRPPATGIT
CCCCEEEEECCCCCCCCCCEEEECHHEEEHHCCCCCEEEEEECCCCCCCCCCCCCCCCCC
GGGPAGGRVGGAVAGGARAGAAGTGDGPGPFGGGQFRDDRFPAFATGAELGSHNIANALA
CCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHCCCHHHHHHH
AAALARADGVDPAAIGAALASFRPGAHRNVTVGRRGGVRYVDDSKATNPHAAAASLRAYP
HHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHHCCC
SVVWIAGGLNKGLEFDDLVVSARRGLRAVVLIGQCADEVAAALARHAPDVPVERAAGMDD
CEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCCHHHHCCCCH
AVDAAAKLAAPGDTVLLAPAAASMDMFRDYAERGDLFAAAVRARGG
HHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCEEEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA