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The map label for this gene is yciL [C]

Identifier: 158316819

GI number: 158316819

Start: 6060068

End: 6060847

Strand: Reverse

Name: yciL [C]

Synonym: Franean1_5061

Alternate gene names: 158316819

Gene position: 6060847-6060068 (Counterclockwise)

Preceding gene: 158316820

Following gene: 158316818

Centisome position: 67.48

GC content: 73.33

Gene sequence:

>780_bases
ATGAACGGCACCGAATCCGAGTCCCCCGACACCCGCCGCCGGGACCCGGCGTCCGAGGGCATCCGCCTGCAGAAGGTGCT
GGCCTCCGCCGGCATCGGCTCTCGCCGGCACAACGAGGAACTGATCGACTCCGGTCGCGTCCGGGTCGACGGCGAGGTGG
TCCGCGAGCAGGGCCGTCGGGTCGACCCGGAGACGGCGGTGATCGAGGTCGACGGCGAGCGCGTCGTCACCCGCACCGGG
CTGGTGCACCTGGCGCTGAACAAGCCGCGCGGTGTGCTGTCGGCGATGTCCGACGACCGCGGCCGGCCGACCATCGCCGA
CTTTCTCACCGAGTTCGGCTCCGTCGGCCTGTTCCACGTCGGCCGGCTGGACGGCGACAGCGAGGGCCTGCTGCTCGTCA
CCAACGACGGCGATCTCGCCCACCGGCTCACCCACCCGTCCTTCGGCGTGCCGAAGACCTACCTGGTCGAGATCAGCGGC
CCGGTCCGCCGCGACGTCCCGCGCCGGCTGCGCTCGGGGGTGACCCTCGAGGACGGCCCCGTCTCGGTCGACGCCGCCCG
GATCGTCGACGTCGCCGCGTCCCGGGTCATGATGGAGCTCACCCTGCACGAGGGCCGCAAGCACGTGGTCCGCCGCATGA
TGGAGGCCGTGGGGCACCCGGTGCACCGCCTCGTCCGGGTCTCGTTCGGCCCGGTGACGCTCGGCACGCTGCGGGCCGGC
CGCGCCCGCCATCTCACCCGGCACGAGGTGGGGGCGCTCTACAAGGCGACCGGGCTGTGA

Upstream 100 bases:

>100_bases
ACTACTTCCTGGAACGCATGGGCCTGCGTGACCTGGACGAACTGCCCCCGCTCGCACCCCTGCTGCCGGGTGTGGACGAT
GTCGATGACATCGTGGCGTC

Downstream 100 bases:

>100_bases
GTCCCGGCGAGCCCCCCACGGCGGCCGGGCGGTTCTCGCCGCCCTACGTCCGCCCGGGGGTCGAGGCGGCCGCAGGGGGC
TCGTCCGCGTGGGATCCGGC

Product: pseudouridine synthase

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MNGTESESPDTRRRDPASEGIRLQKVLASAGIGSRRHNEELIDSGRVRVDGEVVREQGRRVDPETAVIEVDGERVVTRTG
LVHLALNKPRGVLSAMSDDRGRPTIADFLTEFGSVGLFHVGRLDGDSEGLLLVTNDGDLAHRLTHPSFGVPKTYLVEISG
PVRRDVPRRLRSGVTLEDGPVSVDAARIVDVAASRVMMELTLHEGRKHVVRRMMEAVGHPVHRLVRVSFGPVTLGTLRAG
RARHLTRHEVGALYKATGL

Sequences:

>Translated_259_residues
MNGTESESPDTRRRDPASEGIRLQKVLASAGIGSRRHNEELIDSGRVRVDGEVVREQGRRVDPETAVIEVDGERVVTRTG
LVHLALNKPRGVLSAMSDDRGRPTIADFLTEFGSVGLFHVGRLDGDSEGLLLVTNDGDLAHRLTHPSFGVPKTYLVEISG
PVRRDVPRRLRSGVTLEDGPVSVDAARIVDVAASRVMMELTLHEGRKHVVRRMMEAVGHPVHRLVRVSFGPVTLGTLRAG
RARHLTRHEVGALYKATGL
>Mature_259_residues
MNGTESESPDTRRRDPASEGIRLQKVLASAGIGSRRHNEELIDSGRVRVDGEVVREQGRRVDPETAVIEVDGERVVTRTG
LVHLALNKPRGVLSAMSDDRGRPTIADFLTEFGSVGLFHVGRLDGDSEGLLLVTNDGDLAHRLTHPSFGVPKTYLVEISG
PVRRDVPRRLRSGVTLEDGPVSVDAARIVDVAASRVMMELTLHEGRKHVVRRMMEAVGHPVHRLVRVSFGPVTLGTLRAG
RARHLTRHEVGALYKATGL

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=218, Percent_Identity=42.2018348623853, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=36.7521367521368, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=38.2022471910112, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790453, Length=237, Percent_Identity=32.0675105485232, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 28309; Mature: 28309

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGTESESPDTRRRDPASEGIRLQKVLASAGIGSRRHNEELIDSGRVRVDGEVVREQGRR
CCCCCCCCCCCCCCCCHHHCHHHHHHHHHCCCCCCCCCHHHHHCCCEEECHHHHHHCCCC
VDPETAVIEVDGERVVTRTGLVHLALNKPRGVLSAMSDDRGRPTIADFLTEFGSVGLFHV
CCCCEEEEEECCCEEEEECCEEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHCCCCEEEE
GRLDGDSEGLLLVTNDGDLAHRLTHPSFGVPKTYLVEISGPVRRDVPRRLRSGVTLEDGP
ECCCCCCCCEEEEECCCCHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCC
VSVDAARIVDVAASRVMMELTLHEGRKHVVRRMMEAVGHPVHRLVRVSFGPVTLGTLRAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEHHHCCC
RARHLTRHEVGALYKATGL
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNGTESESPDTRRRDPASEGIRLQKVLASAGIGSRRHNEELIDSGRVRVDGEVVREQGRR
CCCCCCCCCCCCCCCCHHHCHHHHHHHHHCCCCCCCCCHHHHHCCCEEECHHHHHHCCCC
VDPETAVIEVDGERVVTRTGLVHLALNKPRGVLSAMSDDRGRPTIADFLTEFGSVGLFHV
CCCCEEEEEECCCEEEEECCEEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHCCCCEEEE
GRLDGDSEGLLLVTNDGDLAHRLTHPSFGVPKTYLVEISGPVRRDVPRRLRSGVTLEDGP
ECCCCCCCCEEEEECCCCHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCC
VSVDAARIVDVAASRVMMELTLHEGRKHVVRRMMEAVGHPVHRLVRVSFGPVTLGTLRAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEHHHCCC
RARHLTRHEVGALYKATGL
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]