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The map label for this gene is gsiB [H]

Identifier: 158316615

GI number: 158316615

Start: 5817523

End: 5819100

Strand: Reverse

Name: gsiB [H]

Synonym: Franean1_4852

Alternate gene names: 158316615

Gene position: 5819100-5817523 (Counterclockwise)

Preceding gene: 158316620

Following gene: 158316609

Centisome position: 64.79

GC content: 68.88

Gene sequence:

>1578_bases
ATGATCCGTACGAGGCGATTACTCGCCACCGCCGCCGTGTGCGCCGTGGCGATGTTCCTGGGGGCGTGCGGTGGTGGCAG
CGATGGCGACGGCGCCACGGGCGTCGCCGCCACGGGTGCCACCGGGGACCCGGTGGCGGGCGGCAAGGGACGGATCCTCA
TGCTGGCCGACACCAGCTCCATGGACCCGGCCAAGCTCGGCAACGCCTACGCGGCGACGGCTGTCCTGGGCAACGCCCTG
TACGGGACGCTGATGATCGACGACGACAAGGGCCAGGTCGTCTACAAGATGGCCGAGTCGTTCACGACCAGCGACAACGG
CTCCACCTTCGAGCTCCGGCTGCGGCCGAGGCTGGTCTTCTCCGACGGCAGCCCGCTCGACGCCGCCGCCGTGAAGTTCA
ACTGGGACCGCCTCAAGGACCCCGCGAACGCGTCGAGCTACCGCGGGGACGCCATGATGATCGCCTCCACCGAGGTGGTG
GACGACCGCACCCTGAAGGTCTCGACGGTCGACCCGATGCCGAACTTCGCCGACGTCGTCCTCACCACGGCGATGAACTG
GATCGCCTCGCCCGCGGCCCTGCAACGCGGCGCGCAGGCGTTCGAGCAGAACCCCGTCGGCGCCGGTCCGTTCAAGCTTG
AGCACTGGACCCGGCAGGCCGACATCGACCTGGTGAAGAACCCGCGCTACTGGGACGCGCCCCGGCCCTACCTCGACGCC
CTCACGATCCGCGCGGCCGTCGAGTCCAGGCAGCGTTACAACACCCTGCTCACCGGAGGCGCGGACGTCGCCATCGAGTC
GGACTGGATCAACCTCCACAAAGCCGCGGAGAGCGGCCAGCCGAGCATCAGCAGGCCGGTCAACGGCGGCATCTTCATGG
CGCTCAACATGCGCCGGGCGCCGTTCGACGACATCCGCGCCCGCCAGGCGGTGGCCGCGGCGCTCGACCTGGAGGCGCTG
AACCTCGCGGTCTACGAGGGCGACGGGCAGCCCGCCGAGACGCTGTTCAACGACGCCTCACCCTTCTACTCCCAGACGCC
CCTGAGGCGCGCGGACCGGGCGAGGGCGCAGCGGCTGTTCGACGAGCTCGCCGCCGAGGGCAAGCCGGTGTCCTTCACGT
TCACCAGCTTCCCCAGCTCCGAGAGCCGGGCGATCGCCGAGAACGTTCAGGCCCAGCTCAGCAGCTTCAAGAACGTCAAG
GTGCAGGTCAAGGTCGTCCAGCTCGCCGAGTACGGCAAGATGAACGCGACGCACGACTTCGACACGTTCATCACGTCCGC
GTTCTTCCAGGACCCAGAACCGAAGCTGTACACGGCCTTCCGTGGCGGCAGGGCCACCAATGTGCCCGGGGTCGACGACC
CCCAGCTCAACGAGGCCCTCGGCACCGGCCGGACCGCGTTGGCGGAGGTGGACCGCAAGGCCGCCTACGAAACGGTCCAG
CAGCGGTTGACCGCACTGACCCCCGGGATCTTCCTCCAGCGGGTCGCGCTCAGCGCCGTCTCCGGCAAGAACGTGGGCGG
TCTCGCGCAGTACGGGCTTGGCTCGTTGCTGCCCGAGGAACTGTGGATCAAGAAGTAG

Upstream 100 bases:

>100_bases
CACACGCGGCCAATCGATTCGGCGGTGGCTGCCCTCACACCGCTCCGGCAGCGACCCCGCTTCCGCGGGCCGGACATCTG
GCACTGGAGAGAGGACGACG

Downstream 100 bases:

>100_bases
GGCCTGAGCGGTAGCCCCCTGATCCAGGGCGGGGAGCCGGCAGCCGCCACGGCTGCCGGCTCGTGCCGTGGGTCAGGCGG
GTTCGGGTGCGGTCAGCGCC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MIRTRRLLATAAVCAVAMFLGACGGGSDGDGATGVAATGATGDPVAGGKGRILMLADTSSMDPAKLGNAYAATAVLGNAL
YGTLMIDDDKGQVVYKMAESFTTSDNGSTFELRLRPRLVFSDGSPLDAAAVKFNWDRLKDPANASSYRGDAMMIASTEVV
DDRTLKVSTVDPMPNFADVVLTTAMNWIASPAALQRGAQAFEQNPVGAGPFKLEHWTRQADIDLVKNPRYWDAPRPYLDA
LTIRAAVESRQRYNTLLTGGADVAIESDWINLHKAAESGQPSISRPVNGGIFMALNMRRAPFDDIRARQAVAAALDLEAL
NLAVYEGDGQPAETLFNDASPFYSQTPLRRADRARAQRLFDELAAEGKPVSFTFTSFPSSESRAIAENVQAQLSSFKNVK
VQVKVVQLAEYGKMNATHDFDTFITSAFFQDPEPKLYTAFRGGRATNVPGVDDPQLNEALGTGRTALAEVDRKAAYETVQ
QRLTALTPGIFLQRVALSAVSGKNVGGLAQYGLGSLLPEELWIKK

Sequences:

>Translated_525_residues
MIRTRRLLATAAVCAVAMFLGACGGGSDGDGATGVAATGATGDPVAGGKGRILMLADTSSMDPAKLGNAYAATAVLGNAL
YGTLMIDDDKGQVVYKMAESFTTSDNGSTFELRLRPRLVFSDGSPLDAAAVKFNWDRLKDPANASSYRGDAMMIASTEVV
DDRTLKVSTVDPMPNFADVVLTTAMNWIASPAALQRGAQAFEQNPVGAGPFKLEHWTRQADIDLVKNPRYWDAPRPYLDA
LTIRAAVESRQRYNTLLTGGADVAIESDWINLHKAAESGQPSISRPVNGGIFMALNMRRAPFDDIRARQAVAAALDLEAL
NLAVYEGDGQPAETLFNDASPFYSQTPLRRADRARAQRLFDELAAEGKPVSFTFTSFPSSESRAIAENVQAQLSSFKNVK
VQVKVVQLAEYGKMNATHDFDTFITSAFFQDPEPKLYTAFRGGRATNVPGVDDPQLNEALGTGRTALAEVDRKAAYETVQ
QRLTALTPGIFLQRVALSAVSGKNVGGLAQYGLGSLLPEELWIKK
>Mature_525_residues
MIRTRRLLATAAVCAVAMFLGACGGGSDGDGATGVAATGATGDPVAGGKGRILMLADTSSMDPAKLGNAYAATAVLGNAL
YGTLMIDDDKGQVVYKMAESFTTSDNGSTFELRLRPRLVFSDGSPLDAAAVKFNWDRLKDPANASSYRGDAMMIASTEVV
DDRTLKVSTVDPMPNFADVVLTTAMNWIASPAALQRGAQAFEQNPVGAGPFKLEHWTRQADIDLVKNPRYWDAPRPYLDA
LTIRAAVESRQRYNTLLTGGADVAIESDWINLHKAAESGQPSISRPVNGGIFMALNMRRAPFDDIRARQAVAAALDLEAL
NLAVYEGDGQPAETLFNDASPFYSQTPLRRADRARAQRLFDELAAEGKPVSFTFTSFPSSESRAIAENVQAQLSSFKNVK
VQVKVVQLAEYGKMNATHDFDTFITSAFFQDPEPKLYTAFRGGRATNVPGVDDPQLNEALGTGRTALAEVDRKAAYETVQ
QRLTALTPGIFLQRVALSAVSGKNVGGLAQYGLGSLLPEELWIKK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=338, Percent_Identity=29.585798816568, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1789397, Length=302, Percent_Identity=28.476821192053, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1789966, Length=385, Percent_Identity=25.4545454545455, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1787495, Length=472, Percent_Identity=21.6101694915254, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1787762, Length=519, Percent_Identity=26.5895953757225, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081878, Length=463, Percent_Identity=22.0302375809935, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1789887, Length=480, Percent_Identity=21.6666666666667, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56393; Mature: 56393

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTRRLLATAAVCAVAMFLGACGGGSDGDGATGVAATGATGDPVAGGKGRILMLADTSS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEECCCC
MDPAKLGNAYAATAVLGNALYGTLMIDDDKGQVVYKMAESFTTSDNGSTFELRLRPRLVF
CCHHHHCCHHHHHHHHHHHEEEEEEEECCCCCEEHEEHHHHCCCCCCCEEEEEEEEEEEE
SDGSPLDAAAVKFNWDRLKDPANASSYRGDAMMIASTEVVDDRTLKVSTVDPMPNFADVV
CCCCCCCCEEEEEEHHHCCCCCCCCCCCCCEEEEEEEEEECCCEEEEEECCCCCCHHHHH
LTTAMNWIASPAALQRGAQAFEQNPVGAGPFKLEHWTRQADIDLVKNPRYWDAPRPYLDA
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEHHHHCCCCCCEEECCCCCCCCCCCHHHH
LTIRAAVESRQRYNTLLTGGADVAIESDWINLHKAAESGQPSISRPVNGGIFMALNMRRA
HHHHHHHHHHHHHHHHEECCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
PFDDIRARQAVAAALDLEALNLAVYEGDGQPAETLFNDASPFYSQTPLRRADRARAQRLF
CHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
DELAAEGKPVSFTFTSFPSSESRAIAENVQAQLSSFKNVKVQVKVVQLAEYGKMNATHDF
HHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEHHCCCCCCCCCH
DTFITSAFFQDPEPKLYTAFRGGRATNVPGVDDPQLNEALGTGRTALAEVDRKAAYETVQ
HHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
QRLTALTPGIFLQRVALSAVSGKNVGGLAQYGLGSLLPEELWIKK
HHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHCHHHCCCHHHCCCC
>Mature Secondary Structure
MIRTRRLLATAAVCAVAMFLGACGGGSDGDGATGVAATGATGDPVAGGKGRILMLADTSS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEECCCC
MDPAKLGNAYAATAVLGNALYGTLMIDDDKGQVVYKMAESFTTSDNGSTFELRLRPRLVF
CCHHHHCCHHHHHHHHHHHEEEEEEEECCCCCEEHEEHHHHCCCCCCCEEEEEEEEEEEE
SDGSPLDAAAVKFNWDRLKDPANASSYRGDAMMIASTEVVDDRTLKVSTVDPMPNFADVV
CCCCCCCCEEEEEEHHHCCCCCCCCCCCCCEEEEEEEEEECCCEEEEEECCCCCCHHHHH
LTTAMNWIASPAALQRGAQAFEQNPVGAGPFKLEHWTRQADIDLVKNPRYWDAPRPYLDA
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEHHHHCCCCCCEEECCCCCCCCCCCHHHH
LTIRAAVESRQRYNTLLTGGADVAIESDWINLHKAAESGQPSISRPVNGGIFMALNMRRA
HHHHHHHHHHHHHHHHEECCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
PFDDIRARQAVAAALDLEALNLAVYEGDGQPAETLFNDASPFYSQTPLRRADRARAQRLF
CHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
DELAAEGKPVSFTFTSFPSSESRAIAENVQAQLSSFKNVKVQVKVVQLAEYGKMNATHDF
HHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEHHCCCCCCCCCH
DTFITSAFFQDPEPKLYTAFRGGRATNVPGVDDPQLNEALGTGRTALAEVDRKAAYETVQ
HHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
QRLTALTPGIFLQRVALSAVSGKNVGGLAQYGLGSLLPEELWIKK
HHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHCHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA