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The map label for this gene is mhpA [H]

Identifier: 158316598

GI number: 158316598

Start: 5775501

End: 5777294

Strand: Reverse

Name: mhpA [H]

Synonym: Franean1_4834

Alternate gene names: 158316598

Gene position: 5777294-5775501 (Counterclockwise)

Preceding gene: 158316599

Following gene: 158316597

Centisome position: 64.32

GC content: 73.75

Gene sequence:

>1794_bases
GTGACCGCGCCCGAGCCGGCCGAGGTCGAGGCCGAGGTCGAGGCCGAGGCTGAGGCCATGGCCGACGTCGTCGTCGTCGG
CTACGGGCCGGTCGGCCAGGTAACGGCGATTCTGCTGGCGCGGCGCGGCTGGCGGGTCACAGTCCTGGAGCGATGGCCCC
GGCCGTACCCGATGCCGCGCGCGGTCTCCTTCGACGGCGAGTCGGCCCGCATCCTCGCCGCGGCCGGCGTCGGCCCGGCG
ATGACCGAGTTCGGTGAGCCGTCCCGCGACTACACCTGGCGCAACGCCGCGGGTGACGTGCTGCTGCACGTCGACGTCCC
CGAGCGCGGGCGTTCCGGCTGGCCGGACTCGACGTCGATGTACCAGCCGGCACTGGAGGAGGCGCTCGCGGAGCGGGGCT
CCCGACTGCCGGGCCTGCGGGTGCTGCGCGGGCACCGGGTGGTCGCGCTGACCGAACGGGACGGCCACGTCGAGCTGACG
GCCGTCCTCGACGGCTCGCCGCCGGACCGCACCCTACGCCTCCGCGCCCGTTGGGTGGTGGGCTGCGACGGCGCGAACAG
CTTCGTGCGGACGAGCCTGGGCGTGGAGACCACCGACTTCGCCTACTTCAACGACTGGCTGACCTGTGACGTGGTCCTGC
GCGAACCGGCGGTGCACAGGCCCAACAACCTCCAGATCTGCGACCCGACCCGGCCCCGCACGGCCGTGTCCGCCGGGCCG
GGGCACCGGCGGTGGGAGTTCATGCGGCTACCCGGGGAACCGGCCGAGGAGTTCGGCCGGACGGAGTCGGCGTGGCGGCT
GCTCGGTCTGTTCGGCCTGCACCCGGGCAACGCCACACTGGAACGGCACGCCGTCTACACGTTCCAGGCCAGGTACGTCG
ACCGCTGGCGGGTGGGGCGGGTGCTGCTCGCCGGGGACGCCGCGCACCTGATGCCGCCGTTCGCCGGGCAGGGCATGTGC
TCGGGCTTCCGCGACGCGGCGAATCTCTCCTGGAAGCTCGACCTGGTGCTCGCCGGAACCGCCCCGGCTGAGCTGCTGGA
CACCTACACCCTGGAGCGGCGGGCGCACGTCCAGCACGCGATCGGCATGTCGATGGACCTCGGGCGGGTGATCTGCGAGA
CCGACCCGGCCGCGGCCCGGGACCGGGACGAGGTCATGATCGCGGTGCGTGCCCGGGGGCTGCGGGAGGACCGCGCGCAG
TCCGCCGTCGAGCCGCTGACCGCCGGGTTCCTGCGCGGCGGGGCCGCCGCACACGGCGGTGGCCCCCAGCGTCCCCCGCA
GCCTTCCCGCCCGGGGCGTCCGGGGCCGCCGTCGGTTCCGGTGGGTGACCTCGTGCCGCAGGGACGAGTTCGGGTCGGTG
ACCGCACCGGGCTGTTCGACGAGTTCGTCGAGCCCGGGTTCGTCCTGCTGGCCACCTGGCCGGCCGGCGGGCATCTCCAT
CTCCGCCCCGAGACGGACGCCGCTTTTGCGCTTCTGGGCGGACGGGTGGTCAATGTTGTCCCCGCCACCGATGAGGAGCC
TCCTGCCGTCGTGGCCGGGAGCGCGGACGCGAGCGCAAGAATCACCGTCGTCGACGTCGACGACGTCTATCTCCGCTATC
TCGCCCTCGCCGGCGCCGATGCCGTTCTGGTGCGGCCGGACTTCTACCTCTTCGGCCGTGTCCCGGCGGCGGCACCGCCG
GCCGCGGACACCCGGGCCGGCGGTACAGCGGTACAGCGGTCGGATTCCGTCGAGGAACTCGTCGGTGACCTGCTGGCCGC
GCTCGACGCCCCGATTCCCGTCGGGAAGCATTAG

Upstream 100 bases:

>100_bases
CCATGCGGGTGCTCACCGACCCGGCCGAGCACGCCCGCGTGCTCGCCGACATCCTCCGCCGCCCGCCGGCACCCGCCACC
GCGCCCACCGGCGACGCCCG

Downstream 100 bases:

>100_bases
CTGTTCCGTCCTGGACGGGCCGGGCTCGTCGGGGAAGGGTGCGCCATGGGCTCCCGGGCCGGCAGGAAACGTCCCCGCCA
GGAGCATGCGCGGAGCCGTA

Product: 3-(3-hydroxyphenyl)propionate hydroxylase

Products: tetrachlorohydroquinone; NADP+; chloride

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MTAPEPAEVEAEVEAEAEAMADVVVVGYGPVGQVTAILLARRGWRVTVLERWPRPYPMPRAVSFDGESARILAAAGVGPA
MTEFGEPSRDYTWRNAAGDVLLHVDVPERGRSGWPDSTSMYQPALEEALAERGSRLPGLRVLRGHRVVALTERDGHVELT
AVLDGSPPDRTLRLRARWVVGCDGANSFVRTSLGVETTDFAYFNDWLTCDVVLREPAVHRPNNLQICDPTRPRTAVSAGP
GHRRWEFMRLPGEPAEEFGRTESAWRLLGLFGLHPGNATLERHAVYTFQARYVDRWRVGRVLLAGDAAHLMPPFAGQGMC
SGFRDAANLSWKLDLVLAGTAPAELLDTYTLERRAHVQHAIGMSMDLGRVICETDPAAARDRDEVMIAVRARGLREDRAQ
SAVEPLTAGFLRGGAAAHGGGPQRPPQPSRPGRPGPPSVPVGDLVPQGRVRVGDRTGLFDEFVEPGFVLLATWPAGGHLH
LRPETDAAFALLGGRVVNVVPATDEEPPAVVAGSADASARITVVDVDDVYLRYLALAGADAVLVRPDFYLFGRVPAAAPP
AADTRAGGTAVQRSDSVEELVGDLLAALDAPIPVGKH

Sequences:

>Translated_597_residues
MTAPEPAEVEAEVEAEAEAMADVVVVGYGPVGQVTAILLARRGWRVTVLERWPRPYPMPRAVSFDGESARILAAAGVGPA
MTEFGEPSRDYTWRNAAGDVLLHVDVPERGRSGWPDSTSMYQPALEEALAERGSRLPGLRVLRGHRVVALTERDGHVELT
AVLDGSPPDRTLRLRARWVVGCDGANSFVRTSLGVETTDFAYFNDWLTCDVVLREPAVHRPNNLQICDPTRPRTAVSAGP
GHRRWEFMRLPGEPAEEFGRTESAWRLLGLFGLHPGNATLERHAVYTFQARYVDRWRVGRVLLAGDAAHLMPPFAGQGMC
SGFRDAANLSWKLDLVLAGTAPAELLDTYTLERRAHVQHAIGMSMDLGRVICETDPAAARDRDEVMIAVRARGLREDRAQ
SAVEPLTAGFLRGGAAAHGGGPQRPPQPSRPGRPGPPSVPVGDLVPQGRVRVGDRTGLFDEFVEPGFVLLATWPAGGHLH
LRPETDAAFALLGGRVVNVVPATDEEPPAVVAGSADASARITVVDVDDVYLRYLALAGADAVLVRPDFYLFGRVPAAAPP
AADTRAGGTAVQRSDSVEELVGDLLAALDAPIPVGKH
>Mature_596_residues
TAPEPAEVEAEVEAEAEAMADVVVVGYGPVGQVTAILLARRGWRVTVLERWPRPYPMPRAVSFDGESARILAAAGVGPAM
TEFGEPSRDYTWRNAAGDVLLHVDVPERGRSGWPDSTSMYQPALEEALAERGSRLPGLRVLRGHRVVALTERDGHVELTA
VLDGSPPDRTLRLRARWVVGCDGANSFVRTSLGVETTDFAYFNDWLTCDVVLREPAVHRPNNLQICDPTRPRTAVSAGPG
HRRWEFMRLPGEPAEEFGRTESAWRLLGLFGLHPGNATLERHAVYTFQARYVDRWRVGRVLLAGDAAHLMPPFAGQGMCS
GFRDAANLSWKLDLVLAGTAPAELLDTYTLERRAHVQHAIGMSMDLGRVICETDPAAARDRDEVMIAVRARGLREDRAQS
AVEPLTAGFLRGGAAAHGGGPQRPPQPSRPGRPGPPSVPVGDLVPQGRVRVGDRTGLFDEFVEPGFVLLATWPAGGHLHL
RPETDAAFALLGGRVVNVVPATDEEPPAVVAGSADASARITVVDVDDVYLRYLALAGADAVLVRPDFYLFGRVPAAAPPA
ADTRAGGTAVQRSDSVEELVGDLLAALDAPIPVGKH

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=546, Percent_Identity=29.6703296703297, Blast_Score=213, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.50

Molecular weight: Translated: 64249; Mature: 64118

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPEPAEVEAEVEAEAEAMADVVVVGYGPVGQVTAILLARRGWRVTVLERWPRPYPMPR
CCCCCCCCCEEHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
AVSFDGESARILAAAGVGPAMTEFGEPSRDYTWRNAAGDVLLHVDVPERGRSGWPDSTSM
EEECCCCCCEEEEECCCCHHHHHCCCCCCCCEECCCCCCEEEEEECCCCCCCCCCCCCHH
YQPALEEALAERGSRLPGLRVLRGHRVVALTERDGHVELTAVLDGSPPDRTLRLRARWVV
HHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCEEEEEEECCCCCCCEEEEEEEEEE
GCDGANSFVRTSLGVETTDFAYFNDWLTCDVVLREPAVHRPNNLQICDPTRPRTAVSAGP
EECCCCHHHHHHCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCEEECCCCCCCEECCCC
GHRRWEFMRLPGEPAEEFGRTESAWRLLGLFGLHPGNATLERHAVYTFQARYVDRWRVGR
CCCCEEEEECCCCCHHHHCCCHHHHHHHHHEECCCCCCCEEEEEEEEEEEHHHCCCCCCE
VLLAGDAAHLMPPFAGQGMCSGFRDAANLSWKLDLVLAGTAPAELLDTYTLERRAHVQHA
EEEECCHHHHCCCCCCCCHHCCCHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHH
IGMSMDLGRVICETDPAAARDRDEVMIAVRARGLREDRAQSAVEPLTAGFLRGGAAAHGG
HCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
GPQRPPQPSRPGRPGPPSVPVGDLVPQGRVRVGDRTGLFDEFVEPGFVLLATWPAGGHLH
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEECCCCCCHHHHCCCCEEEEEECCCCCEEE
LRPETDAAFALLGGRVVNVVPATDEEPPAVVAGSADASARITVVDVDDVYLRYLALAGAD
ECCCCCCHHEECCCEEEEEEECCCCCCCEEEECCCCCCEEEEEEECHHHHHHHHHHCCCC
AVLVRPDFYLFGRVPAAAPPAADTRAGGTAVQRSDSVEELVGDLLAALDAPIPVGKH
EEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TAPEPAEVEAEVEAEAEAMADVVVVGYGPVGQVTAILLARRGWRVTVLERWPRPYPMPR
CCCCCCCCEEHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
AVSFDGESARILAAAGVGPAMTEFGEPSRDYTWRNAAGDVLLHVDVPERGRSGWPDSTSM
EEECCCCCCEEEEECCCCHHHHHCCCCCCCCEECCCCCCEEEEEECCCCCCCCCCCCCHH
YQPALEEALAERGSRLPGLRVLRGHRVVALTERDGHVELTAVLDGSPPDRTLRLRARWVV
HHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCEEEEEEECCCCCCCEEEEEEEEEE
GCDGANSFVRTSLGVETTDFAYFNDWLTCDVVLREPAVHRPNNLQICDPTRPRTAVSAGP
EECCCCHHHHHHCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCEEECCCCCCCEECCCC
GHRRWEFMRLPGEPAEEFGRTESAWRLLGLFGLHPGNATLERHAVYTFQARYVDRWRVGR
CCCCEEEEECCCCCHHHHCCCHHHHHHHHHEECCCCCCCEEEEEEEEEEEHHHCCCCCCE
VLLAGDAAHLMPPFAGQGMCSGFRDAANLSWKLDLVLAGTAPAELLDTYTLERRAHVQHA
EEEECCHHHHCCCCCCCCHHCCCHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHH
IGMSMDLGRVICETDPAAARDRDEVMIAVRARGLREDRAQSAVEPLTAGFLRGGAAAHGG
HCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
GPQRPPQPSRPGRPGPPSVPVGDLVPQGRVRVGDRTGLFDEFVEPGFVLLATWPAGGHLH
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEECCCCCCHHHHCCCCEEEEEECCCCCEEE
LRPETDAAFALLGGRVVNVVPATDEEPPAVVAGSADASARITVVDVDDVYLRYLALAGAD
ECCCCCCHHEECCCEEEEEEECCCCCCCEEEECCCCCCEEEEEEECHHHHHHHHHHCCCC
AVLVRPDFYLFGRVPAAAPPAADTRAGGTAVQRSDSVEELVGDLLAALDAPIPVGKH
EEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pentachlorophenol; NADPH; H+; O2

Specific reaction: pentachlorophenol + NADPH + H+ + O2 = tetrachlorohydroquinone + NADP+ + chloride

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10537203 [H]