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The map label for this gene is pur8 [H]

Identifier: 158316416

GI number: 158316416

Start: 5549349

End: 5549828

Strand: Reverse

Name: pur8 [H]

Synonym: Franean1_4650

Alternate gene names: 158316416

Gene position: 5549828-5549349 (Counterclockwise)

Preceding gene: 158316417

Following gene: 158316413

Centisome position: 61.79

GC content: 65.0

Gene sequence:

>480_bases
GTGCCGGACCAGGAGCCCGTGAACACGCCGGAGCAGGCCTCGAGAGACAGCCGTCGATGGTGGATACTGCTGGTGGTCTC
CGCGGCACAGTTCATGGTCGGGCTGGACGCCACGGTCGTCAATGTCATGATGCCCGAGCTGCAGGACTCGCTCGGACTGT
CGGCGACCGGCCTGCAGTGGGTGATGAGTATCTATGTGCTGCTCTTCGGCGGGCTGATGCTGCTCGGCGGGCGGTTGACC
GACGTGATCGGTCGGCGCCGGATACTACTCGCCGGGCTGGCCGAGAGTGTTGCGTGTCAGGGCGTAGAGCAGCGACCACA
CCATGACCATCCAGCCTGCCCGACACGGGCTCCGCCCCGCAGGCAAACACGCAGGTCACCGCTCGAATCCGGGTTCTGGA
GCCCCACAAGGTCGGATAGGCCTCCCGGTAGACGAGTTCGTTCTTCAGGGTGCCGAAGAACGATTCCGCTGCGGAGTTGA

Upstream 100 bases:

>100_bases
TCAGCCTGCGCGGAATGTACGAGCGGGTCGCCACGGCGGTCTGACGGCCGGGTGGCACGAGGAGAATGATGTGAGTATCG
AGGGTCAGGGCGCGGCGCAG

Downstream 100 bases:

>100_bases
TGCTCCTATAGATTTCAAGCGGCCTGAGGCGACGGATCAGAAGCCGCGGCGCGGCTGTACTCGACCACGTTCGATGGCGG
TAGAAGGCGATAGAGTTCCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MPDQEPVNTPEQASRDSRRWWILLVVSAAQFMVGLDATVVNVMMPELQDSLGLSATGLQWVMSIYVLLFGGLMLLGGRLT
DVIGRRRILLAGLAESVACQGVEQRPHHDHPACPTRAPPRRQTRRSPLESGFWSPTRSDRPPGRRVRSSGCRRTIPLRS

Sequences:

>Translated_159_residues
MPDQEPVNTPEQASRDSRRWWILLVVSAAQFMVGLDATVVNVMMPELQDSLGLSATGLQWVMSIYVLLFGGLMLLGGRLT
DVIGRRRILLAGLAESVACQGVEQRPHHDHPACPTRAPPRRQTRRSPLESGFWSPTRSDRPPGRRVRSSGCRRTIPLRS
>Mature_158_residues
PDQEPVNTPEQASRDSRRWWILLVVSAAQFMVGLDATVVNVMMPELQDSLGLSATGLQWVMSIYVLLFGGLMLLGGRLTD
VIGRRRILLAGLAESVACQGVEQRPHHDHPACPTRAPPRRQTRRSPLESGFWSPTRSDRPPGRRVRSSGCRRTIPLRS

Specific function: May be involved in active puromycin efflux energized by a proton-dependent electrochemical gradient. In addition, it could be implicated in secreting N-acetylpuromycin, the last intermediate of the puromycin biosynthesis pathway, to the environment [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 17721; Mature: 17590

Theoretical pI: Translated: 11.73; Mature: 11.73

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDQEPVNTPEQASRDSRRWWILLVVSAAQFMVGLDATVVNVMMPELQDSLGLSATGLQW
CCCCCCCCCCHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VMSIYVLLFGGLMLLGGRLTDVIGRRRILLAGLAESVACQGVEQRPHHDHPACPTRAPPR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
RQTRRSPLESGFWSPTRSDRPPGRRVRSSGCRRTIPLRS
HHHHCCCHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PDQEPVNTPEQASRDSRRWWILLVVSAAQFMVGLDATVVNVMMPELQDSLGLSATGLQW
CCCCCCCCCHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VMSIYVLLFGGLMLLGGRLTDVIGRRRILLAGLAESVACQGVEQRPHHDHPACPTRAPPR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
RQTRRSPLESGFWSPTRSDRPPGRRVRSSGCRRTIPLRS
HHHHCCCHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7916693 [H]